(Circular) (Six-base) MAPSORT of: pFN21AE0669.seq Check: 6554 from: 1 to: 5452 pFN21AE0669 5452 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3262 278 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2795 1937 Size: 858 4594 Acc65I G'GTAC_C Cuts at: 2773 2773 Size: 5452 AclI AA'CG_TT Cuts at: 1563 3579 3952 1563 Size: 2016 373 3063 Fragments arranged by size: 3063 2016 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2578 3515 4899 5167 1632 Size: 946 937 1384 268 1917 Fragments arranged by size: 1917 1384 946 937 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5255 AflIII A'CryG_T Cuts at: 1203 4351 5328 1203 Size: 3148 977 1327 Fragments arranged by size: 3148 1327 977 AgeI A'CCGG_T Cuts at: 4283 4283 Size: 5452 AhdI GACnn_n'nnGTC Cuts at: 4181 4181 Size: 5452 AleI CACnn'nnGTG Cuts at: 1166 2276 1166 Size: 1110 4342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2294 2432 1184 Size: 1110 138 4204 Fragments arranged by size: 4204 1110 138 AlwNI CAG_nnn'CTG Cuts at: 2383 2440 3195 4767 2383 Size: 57 755 1572 3068 Fragments arranged by size: 3068 1572 755 57 ApaI G_GGCC'C Cuts at: 2658 2716 2658 Size: 58 5394 ApaLI G'TGCA_C Cuts at: 3509 4665 3509 Size: 1156 4296 ApoI r'AATT_y Cuts at: 1493 2761 2987 3139 5248 1493 Size: 1268 226 152 2109 1697 Fragments arranged by size: 2109 1697 1268 226 152 AseI AT'TA_AT Cuts at: 160 1032 4006 160 Size: 872 2974 1606 Fragments arranged by size: 2974 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 AvaI C'yCGr_G Cuts at: 1870 1943 2262 2606 2777 1870 Size: 73 319 344 171 4545 Fragments arranged by size: 4545 344 319 171 73 BamHI G'GATC_C Cuts at: 2782 3149 2782 Size: 367 5085 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2644 2773 4228 618 Size: 325 217 627 857 129 1455 1842 Fragments arranged by size: 1842 1455 857 627 325 217 129 BanII G_rGCy'C Cuts at: 1391 2175 2613 2658 2716 2771 1391 Size: 784 438 45 58 55 4072 Fragments arranged by size: 4072 784 438 58 55 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2141 2175 3643 3677 1903 Size: 34 204 34 1468 34 3678 Fragments arranged by size: 3678 1468 204 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4554 3344 Size: 1210 4242 BclI T'GATC_A Cuts at: 1548 1548 Size: 5452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2533 4063 136 Size: 107 122 71 631 605 861 1530 1525 Fragments arranged by size: 1530 1525 861 631 605 122 107 71 BglII A'GATC_T Cuts at: 5447 5447 Size: 5452 BlpI GC'TnA_GC Cuts at: 2732 2869 2732 Size: 137 5315 Bme1580I G_kGCm'C Cuts at: 1790 2545 2658 2716 3513 4669 1790 Size: 755 113 58 797 1156 2573 Fragments arranged by size: 2573 1156 797 755 113 58 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5452 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2428 2471 2532 Size: 416 53 83 399 24 1011 43 61 Cuts at: 2532 3183 4141 442 Size: 651 958 1753 Fragments arranged by size: 1753 1011 958 651 416 399 83 61 53 43 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 2393 2596 4112 1239 Size: 974 180 203 1516 2579 Fragments arranged by size: 2579 1516 974 203 180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3577 4442 4740 3577 Size: 865 298 4289 Fragments arranged by size: 4289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4115 882 Size: 617 2616 2219 Fragments arranged by size: 2616 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 2396 3243 493 Size: 711 1074 118 847 2702 Fragments arranged by size: 2702 1074 847 711 118 BsaBI GATnn'nnATC Cuts at: 5446 5446 Size: 5452 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2049 2181 3259 3641 Size: 53 83 186 936 516 132 1078 382 Cuts at: 3641 4276 275 Size: 635 1451 Fragments arranged by size: 1451 1078 936 635 516 382 186 132 83 53 BsaWI w'CCGG_w Cuts at: 3885 4283 4557 4704 5115 3885 Size: 398 274 147 411 4222 Fragments arranged by size: 4222 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5227 Fragments arranged by size: 5227 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 1186 Size: 1057 4395 BseYI C'CCAG_C Cuts at: 2313 2421 2427 2500 2512 4655 5151 2313 Size: 108 6 73 12 2143 496 2614 Fragments arranged by size: 2614 2143 496 108 73 12 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2185 2669 1163 Size: 573 449 484 3946 Fragments arranged by size: 3946 573 484 449 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2902 3663 4691 1540 Size: 275 186 901 761 1028 2301 Fragments arranged by size: 2301 1028 901 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2582 3513 3598 4669 2582 Size: 931 85 1071 3365 Fragments arranged by size: 3365 1071 931 85 BsmI GAATG_Cn' Cuts at: 2963 3056 2963 Size: 93 5359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2545 2582 2613 2658 2716 2771 Size: 399 385 370 37 31 45 58 55 Cuts at: 2771 3513 3598 4669 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 385 370 85 58 55 45 37 31 BspEI T'CCGG_A Cuts at: 5115 5115 Size: 5452 BspHI T'CATG_A Cuts at: 3341 5071 3341 Size: 1730 3722 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2358 2807 844 Size: 1514 449 3489 Fragments arranged by size: 3489 1514 449 BsrBI CCG'CTC Cuts at: 1394 1823 3339 1394 Size: 429 1516 3507 Fragments arranged by size: 3507 1516 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3947 4121 65 Size: 1183 2699 174 1396 Fragments arranged by size: 2699 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 2532 4096 4283 1700 Size: 110 299 423 1564 187 2869 Fragments arranged by size: 2869 1564 423 299 187 110 BsrGI T'GTAC_A Cuts at: 96 2076 96 Size: 1980 3472 BssHII G'CGCG_C Cuts at: 1925 2267 1925 Size: 342 5110 BssSI C'ACGA_G Cuts at: 2687 3512 4524 2687 Size: 825 1012 3615 Fragments arranged by size: 3615 1012 825 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5452 BstBI TT'CG_AA Cuts at: 4256 4256 Size: 5452 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5452 BstYI r'GATC_y Cuts at: 1972 2706 2782 3149 3534 3551 5003 5089 5101 Size: 734 76 367 385 17 1452 86 12 Cuts at: 5101 5447 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 734 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2175 2304 2650 513 Size: 552 1110 129 346 3315 Fragments arranged by size: 3315 1110 552 346 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4713 Fragments arranged by size: 4713 683 56 BtsI GCAGTG_nn' Cuts at: 2968 3762 3782 2968 Size: 794 20 4638 Fragments arranged by size: 4638 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2068 2103 529 Size: 35 1504 35 3878 Fragments arranged by size: 3878 1504 35 35 DraI TTT'AAA Cuts at: 2756 2756 Size: 5452 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4459 809 Size: 507 3143 1802 Fragments arranged by size: 3143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 2698 2722 Size: 54 1425 50 275 18 453 415 24 Cuts at: 2722 2899 3788 5417 8 Size: 177 889 1629 43 Fragments arranged by size: 1629 1425 889 453 415 275 177 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2899 1537 Size: 275 1087 4090 Fragments arranged by size: 4090 1087 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2147 3382 1353 Size: 794 1235 3423 Fragments arranged by size: 3423 1235 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4039 4413 4559 387 Size: 1064 138 2450 374 146 1280 Fragments arranged by size: 2450 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 2393 2578 2596 3515 4112 4899 Size: 393 581 180 185 18 919 597 787 Cuts at: 4899 5167 1239 Size: 268 1524 Fragments arranged by size: 1524 919 787 597 581 393 268 185 180 18 EcoO109I rG'GnC_Cy Cuts at: 2259 2323 2655 2713 2259 Size: 64 332 58 4998 Fragments arranged by size: 4998 332 64 58 EcoRI G'AATT_C Cuts at: 2761 3139 2761 Size: 378 5074 FspI TGC'GCA Cuts at: 3958 3958 Size: 5452 HaeII r_GCGC'y Cuts at: 1678 2116 4599 1678 Size: 438 2483 2531 Fragments arranged by size: 2531 2483 438 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4093 4125 4167 4199 1373 Size: 32 135 32 2521 32 42 32 2626 Fragments arranged by size: 2626 2521 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2796 3042 669 Size: 988 281 858 246 3079 Fragments arranged by size: 3079 988 858 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5452 HpaI GTT'AAC Cuts at: 3042 3042 Size: 5452 KpnI G_GTAC'C Cuts at: 2777 2777 Size: 5452 MfeI C'AATT_G Cuts at: 3051 3051 Size: 5452 MluI A'CGCG_T Cuts at: 5328 5328 Size: 5452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4566 4750 1305 Size: 3261 184 2007 Fragments arranged by size: 3261 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2700 2724 10 Size: 54 1425 343 868 24 2738 Fragments arranged by size: 2738 1425 868 343 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2276 3410 3769 3928 518 Size: 648 1110 1134 359 159 2042 Fragments arranged by size: 2042 1134 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2288 2383 2868 3545 4693 4938 1606 Size: 682 95 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 682 677 485 245 95 NaeI GCC'GGC Cuts at: 2534 2534 Size: 5452 NcoI C'CATG_G Cuts at: 513 1065 2304 513 Size: 552 1239 3661 Fragments arranged by size: 3661 1239 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5452 NgoMIV G'CCGG_C Cuts at: 2532 2532 Size: 5452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5452 NotI GC'GGCC_GC Cuts at: 2899 2899 Size: 5452 NspI r_CATG'y Cuts at: 1132 1229 2140 2810 4355 1132 Size: 97 911 670 1545 2229 Fragments arranged by size: 2229 1545 911 670 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5452 PciI A'CATG_T Cuts at: 4351 4351 Size: 5452 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5452 PfoI T'CCnGG_A Cuts at: 2190 2190 Size: 5452 PmeI GTTT'AAAC Cuts at: 2756 2756 Size: 5452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3521 3553 5034 5066 3521 Size: 32 1481 32 3907 Fragments arranged by size: 3907 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2259 2259 Size: 5452 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5452 PsiI TTA'TAA Cuts at: 3022 3022 Size: 5452 PspOMI G'GGCC_C Cuts at: 2654 2712 2654 Size: 58 5394 PstI C_TGCA'G Cuts at: 830 2804 830 Size: 1974 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5452 PvuII CAG'CTG Cuts at: 2383 2383 Size: 5452 SalI G'TCGA_C Cuts at: 1936 2794 1936 Size: 858 4594 SanDI GG'GwC_CC Cuts at: 2259 2259 Size: 5452 SapI GCTCTTCn'nnn_ Cuts at: 2147 2147 Size: 5452 SbfI CC_TGCA'GG Cuts at: 2804 2804 Size: 5452 ScaI AGT'ACT Cuts at: 3700 5412 3700 Size: 1712 3740 SfcI C'TryA_G Cuts at: 826 1045 2800 3935 4616 4807 826 Size: 219 1755 1135 681 191 1471 Fragments arranged by size: 1755 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5452 SgrAI Cr'CCGG_yG Cuts at: 1700 2109 1700 Size: 409 5043 SmaI CCC'GGG Cuts at: 1872 2264 2779 1872 Size: 392 515 4545 Fragments arranged by size: 4545 515 392 SmlI C'TyrA_G Cuts at: 820 1017 1943 3556 4457 4719 820 Size: 197 926 1613 901 262 1553 Fragments arranged by size: 1613 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3243 493 Size: 2750 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5452 SphI G_CATG'C Cuts at: 1132 2810 1132 Size: 1678 3774 SspI AAT'ATT Cuts at: 5 52 3376 5 Size: 47 3324 2081 Fragments arranged by size: 3324 2081 47 StuI AGG'CCT Cuts at: 1517 2599 1517 Size: 1082 4370 StyI C'CwwG_G Cuts at: 513 1065 2304 2553 2891 513 Size: 552 1239 249 338 3074 Fragments arranged by size: 3074 1239 552 338 249 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3479 3496 3649 3834 4279 325 Size: 1255 1899 17 153 185 445 1498 Fragments arranged by size: 1899 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 3698 5410 Size: 274 80 33 51 1443 99 1622 1712 Cuts at: 5410 96 Size: 138 Fragments arranged by size: 1712 1622 1443 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5412 5444 5412 Size: 32 5420 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5452 XbaI T'CTAG_A Cuts at: 2788 2788 Size: 5452 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5452 XmaI C'CCGG_G Cuts at: 1870 2262 2777 1870 Size: 392 515 4545 Fragments arranged by size: 4545 515 392 XmnI GAAnn'nnTTC Cuts at: 3581 3581 Size: 5452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3260 276 Size: 53 83 186 936 1726 2468 Fragments arranged by size: 2468 1726 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI