Restriction map for
pFN21AE4349
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BcgI BciVI
BclI BcnI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI HpaII
KasI KpnI Ksp632I KspI MamI
MboI MflI MluI MluNI MroI
MscI MspI MvnI NaeI NarI
NciI NcoI NdeI NdeII NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 857 GTCTAC GT^[AC][GT]AC
AfaI 765 GTAC GT^AC
AflII 21 CTTAAG C^TTAAG
AlwNI 44 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 798 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 694 CCTCAGC CCTCAGC(-5/-2)
BbvI 998 GCAGC GCAGC(8/12)
BfaI 847 CTAG C^TAG
BfrI 21 CTTAAG C^TTAAG
BglI 170 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 893 CTGGAG CTGGAG(16/14)
BsmFI 242 GGGAC GGGAC(10/14)
BspMI 82 GCAGGT ACCTGC(4/8)
BsrDI 942 CATTGC GCAATG(2/0)
BssKI 653 CCAGG ^CC[ACGT]GG
BstBI 431 TTCGAA TT^CGAA
BstNI 653 CCAGG CC^[AT]GG
BstXI 108 CCACTACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 699 GCCAGC GC[ACGT]^[ACGT]GC
DraII 242 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 866 CGGCCA [CT]^GGCC[GA]
EcoO109I 242 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 653 CCAGG ^CC[AT]GG
EcoT14I 380 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
Fnu4HI 998 GCAGC GC^[ACGT]GC
HindIII 1013 AAGCTT A^AGCTT
ItaI 998 GCAGC GC^[ACGT]GC
MaeI 847 CTAG C^TAG
MaeII 203 ACGT A^CGT
MfeI 398 CAATTG C^AATTG
MspA1I 284 CAGCTG C[AC]G^C[GT]G
MunI 398 CAATTG C^AATTG
MvaI 653 CCAGG CC^[AT]GG
MwoI 170 GCCAAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 1 ATGCAT ATGCA^T
NspI 166 ACATGC [GA]CATG^[CT]
NspV 431 TTCGAA TT^CGAA
PflFI 798 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 131 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 242 GGGACCT [GA]G^G[AT]CC[CT]
PstI 724 CTGCAG CTGCA^G
PvuII 284 CAGCTG CAG^CTG
RsaI 765 GTAC GT^AC
ScrFI 653 CCAGG CC^[ACGT]GG
SfaNI 3 GCATC GCATC(5/9)
SfuI 431 TTCGAA TT^CGAA
StyI 380 CCAAGG C^C[AT][AT]GG
TaqI 432 TCGA T^CGA
TseI 998 GCAGC G^C[AT]GC
Tth111I 798 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 432 TCGA T^CGA
Van91I 131 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 846 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 322 GAGCAC G[AT]GC[AT]^C
438 GAGCAC
BbsI 1051 GAAGAC GAAGAC(2/6)
804 GTCTTC
BmyI 322 GAGCAC G[AGT]GC[ACT]^C
438 GAGCAC
BpuAI 1051 GAAGAC GAAGAC(2/6)
804 GTCTTC
BsiHKAI 322 GAGCAC G[AT]GC[AT]^C
438 GAGCAC
BsiYI 33 CCTACATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
131 CCATGTGCTGG
BslI 33 CCTACATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
131 CCATGTGCTGG
BsmAI 262 GTCTC GTCTC(1/5)
622 GTCTC
BsmI 512 GCATTC GAATGC(1/-1)
584 GCATTC
Bsp1286I 322 GAGCAC G[AGT]GC[ACT]^C
438 GAGCAC
BsrI 607 CCAGT ACTGG(1/-1)
670 CCAGT
DdeI 695 CTCAG C^T[ACGT]AG
982 CTAAG
DsaI 327 CCGTGG C^C[GA][CT]GG
1085 CCACGG
HaeIII 814 GGCC GG^CC
867 GGCC
MseI 22 TTAA T^TAA
1010 TTAA
NlaIV 196 GGAACC GG[ACGT]^[ACGT]CC
242 GGGACC
PleI 802 GAGTC GAGTC(4/5)
896 GAGTC
SmlI 8 CTCAAG C^T[CT][GA]AG
21 CTTAAG
TfiI 46 GATTC G^A[AT]TC
884 GAATC
Tru9I 22 TTAA T^TAA
1010 TTAA
Tsp509I 399 AATT ^AATT
1008 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI AvaII BsaJI BstF5I Cfr13I
FokI HinfI HphI MaeIII MboII
MnlI MslI NlaIII Sau96I SfcI
Tsp45I TspRI