(Circular) (Six-base) MAPSORT of: pFN21AE4349.seq Check: 6501 from: 1 to: 5797 pFN21AE4349 5797 bp With 182 enzymes: SgfI * March 28, 2012 13:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3607 278 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2863 3140 1937 Size: 926 277 4594 Fragments arranged by size: 4594 926 277 Acc65I G'GTAC_C Cuts at: 3118 3118 Size: 5797 AclI AA'CG_TT Cuts at: 1563 3924 4297 1563 Size: 2361 373 3063 Fragments arranged by size: 3063 2361 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2796 3860 5244 5512 1632 Size: 1164 1064 1384 268 1917 Fragments arranged by size: 1917 1384 1164 1064 268 AflII C'TTAA_G Cuts at: 820 1017 2026 820 Size: 197 1009 4591 Fragments arranged by size: 4591 1009 197 AflIII A'CryG_T Cuts at: 1203 4696 5673 1203 Size: 3493 977 1327 Fragments arranged by size: 3493 1327 977 AgeI A'CCGG_T Cuts at: 4628 4628 Size: 5797 AhdI GACnn_n'nnGTC Cuts at: 4526 4526 Size: 5797 AleI CACnn'nnGTG Cuts at: 1166 2118 1166 Size: 952 4845 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5797 AlwNI CAG_nnn'CTG Cuts at: 2054 3540 5112 2054 Size: 1486 1572 2739 Fragments arranged by size: 2739 1572 1486 ApaLI G'TGCA_C Cuts at: 3854 5010 3854 Size: 1156 4641 ApoI r'AATT_y Cuts at: 1493 3106 3332 3484 5593 1493 Size: 1613 226 152 2109 1697 Fragments arranged by size: 2109 1697 1613 226 152 AseI AT'TA_AT Cuts at: 160 1032 4351 160 Size: 872 3319 1606 Fragments arranged by size: 3319 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 AvaI C'yCGr_G Cuts at: 1870 1943 3122 1870 Size: 73 1179 4545 Fragments arranged by size: 4545 1179 73 BamHI G'GATC_C Cuts at: 3127 3494 3127 Size: 367 5430 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3118 4573 618 Size: 325 217 627 1331 1455 1842 Fragments arranged by size: 1842 1455 1331 627 325 217 BanII G_rGCy'C Cuts at: 1391 3116 1391 Size: 1725 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2802 3063 928 Size: 971 903 261 3662 Fragments arranged by size: 3662 971 903 261 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 5797 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3988 4022 1903 Size: 34 50 34 1967 34 3678 Fragments arranged by size: 3678 1967 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3689 4899 3689 Size: 1210 4587 BclI T'GATC_A Cuts at: 1548 1548 Size: 5797 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5797 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2181 4408 136 Size: 107 122 71 631 605 509 2227 1525 Fragments arranged by size: 2227 1525 631 605 509 122 107 71 BglII A'GATC_T Cuts at: 5792 5792 Size: 5797 BlpI GC'TnA_GC Cuts at: 3214 3214 Size: 5797 Bme1580I G_kGCm'C Cuts at: 1790 3858 5014 1790 Size: 2068 1156 2573 Fragments arranged by size: 2573 2068 1156 BmgBI CAC'GTC Cuts at: 1318 2209 1318 Size: 891 4906 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3528 4486 442 Size: 416 53 83 399 24 2111 958 1753 Fragments arranged by size: 2111 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5797 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2919 4457 1239 Size: 1680 1538 2579 Fragments arranged by size: 2579 1680 1538 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 5797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1998 3922 4787 5085 1998 Size: 1924 865 298 2710 Fragments arranged by size: 2710 1924 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4460 882 Size: 617 2961 2219 Fragments arranged by size: 2961 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3588 493 Size: 711 2384 2702 Fragments arranged by size: 2702 2384 711 BsaBI GATnn'nnATC Cuts at: 5791 5791 Size: 5797 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3604 3986 4621 275 Size: 53 83 186 936 2071 382 635 1451 Fragments arranged by size: 2071 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4230 4628 4902 5049 5460 4230 Size: 398 274 147 411 4567 Fragments arranged by size: 4567 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2383 2413 1178 Size: 30 165 30 980 30 4562 Fragments arranged by size: 4562 980 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5797 BseYI C'CCAG_C Cuts at: 5000 5496 5000 Size: 496 5301 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5224 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 4008 5036 1540 Size: 275 186 1246 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2331 2447 3858 3943 5014 2331 Size: 116 1411 85 1071 3114 Fragments arranged by size: 3114 1411 1071 116 85 BsmI GAATG_Cn' Cuts at: 2517 2589 3308 3401 2517 Size: 72 719 93 4913 Fragments arranged by size: 4913 719 93 72 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2331 2447 3116 3858 3943 5014 1391 Size: 399 541 116 669 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 669 541 399 116 85 BspEI T'CCGG_A Cuts at: 5460 5460 Size: 5797 BspHI T'CATG_A Cuts at: 3686 5416 3686 Size: 1730 4067 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2078 3152 844 Size: 1234 1074 3489 Fragments arranged by size: 3489 1234 1074 BsrBI CCG'CTC Cuts at: 1394 1823 3684 1394 Size: 429 1861 3507 Fragments arranged by size: 3507 1861 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2946 4292 4466 65 Size: 1183 1698 1346 174 1396 Fragments arranged by size: 1698 1396 1346 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4441 4628 1700 Size: 110 2631 187 2869 Fragments arranged by size: 2869 2631 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5797 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5797 BssSI C'ACGA_G Cuts at: 3857 4869 3857 Size: 1012 4785 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5797 BstBI TT'CG_AA Cuts at: 2437 4601 2437 Size: 2164 3633 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5797 BstXI CCAn_nnnn'nTGG Cuts at: 2120 2120 Size: 5797 BstYI r'GATC_y Cuts at: 1972 3127 3494 3879 3896 5348 5434 5446 5792 Size: 1155 367 385 17 1452 86 12 346 Cuts at: 5792 1972 Size: 1977 Fragments arranged by size: 1977 1452 1155 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2332 3090 513 Size: 552 1267 758 3220 Fragments arranged by size: 3220 1267 758 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5058 Fragments arranged by size: 5058 683 56 BtsI GCAGTG_nn' Cuts at: 2726 3313 4107 4127 2726 Size: 587 794 20 4396 Fragments arranged by size: 4396 794 587 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5797 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5762 DraI TTT'AAA Cuts at: 3101 3101 Size: 5797 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4804 809 Size: 507 3488 1802 Fragments arranged by size: 3488 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2871 3244 4133 Size: 54 1425 50 275 18 1041 373 889 Cuts at: 4133 5762 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1041 889 373 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 1537 Size: 275 1432 4090 Fragments arranged by size: 4090 1432 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3727 1353 Size: 2374 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4384 4758 4904 387 Size: 1064 138 2795 374 146 1280 Fragments arranged by size: 2795 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2796 2919 3860 4457 5244 5512 1239 Size: 393 1164 123 941 597 787 268 1524 Fragments arranged by size: 1524 1164 941 787 597 393 268 123 EcoO109I rG'GnC_Cy Cuts at: 2248 2248 Size: 5797 EcoRI G'AATT_C Cuts at: 3106 3484 3106 Size: 378 5419 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2980 2980 Size: 5797 FspI TGC'GCA Cuts at: 4303 4303 Size: 5797 HaeII r_GCGC'y Cuts at: 1678 4944 1678 Size: 3266 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4438 4470 4512 4544 1373 Size: 32 135 32 2866 32 42 32 2626 Fragments arranged by size: 2866 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3387 669 Size: 988 281 1203 246 3079 Fragments arranged by size: 3079 1203 988 281 246 HindIII A'AGCT_T Cuts at: 748 3018 748 Size: 2270 3527 HpaI GTT'AAC Cuts at: 3387 3387 Size: 5797 KpnI G_GTAC'C Cuts at: 3122 3122 Size: 5797 MfeI C'AATT_G Cuts at: 2403 3396 2403 Size: 993 4804 MluI A'CGCG_T Cuts at: 5673 5673 Size: 5797 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2444 2480 2513 2528 2546 2600 2618 4911 Size: 1139 36 33 15 18 54 18 2293 Cuts at: 4911 5095 1305 Size: 184 2007 Fragments arranged by size: 2293 2007 1139 184 54 36 33 18 18 15 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3975 Fragments arranged by size: 3975 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2118 2135 2137 3755 4114 4273 518 Size: 648 952 17 2 1618 359 159 2042 Fragments arranged by size: 2042 1618 952 648 359 159 17 2 MspA1I CmG'CkG Cuts at: 1606 2291 3213 3890 5038 5283 1606 Size: 685 922 677 1148 245 2120 Fragments arranged by size: 2120 1148 922 685 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5245 NdeI CA'TA_TG Cuts at: 387 387 Size: 5797 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5797 NotI GC'GGCC_GC Cuts at: 3244 3244 Size: 5797 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5797 NspI r_CATG'y Cuts at: 1132 1229 2175 3155 4700 1132 Size: 97 946 980 1545 2229 Fragments arranged by size: 2229 1545 980 946 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5797 PciI A'CATG_T Cuts at: 4696 4696 Size: 5797 PflMI CCAn_nnn'nTGG Cuts at: 1108 2142 1108 Size: 1034 4763 PmeI GTTT'AAAC Cuts at: 3101 3101 Size: 5797 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3866 3898 5379 5411 3866 Size: 32 1481 32 4252 Fragments arranged by size: 4252 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2248 Size: 5797 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5797 PsiI TTA'TAA Cuts at: 3367 3367 Size: 5797 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2847 2879 2847 Size: 32 5765 PstI C_TGCA'G Cuts at: 830 2733 3149 830 Size: 1903 416 3478 Fragments arranged by size: 3478 1903 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5797 PvuII CAG'CTG Cuts at: 2291 2291 Size: 5797 SalI G'TCGA_C Cuts at: 1936 3139 1936 Size: 1203 4594 SbfI CC_TGCA'GG Cuts at: 3149 3149 Size: 5797 ScaI AGT'ACT Cuts at: 4045 5757 4045 Size: 1712 4085 SfcI C'TryA_G Cuts at: 826 1045 2055 2116 2729 3145 4280 4961 5152 Size: 219 1010 61 613 416 1135 681 191 Cuts at: 5152 826 Size: 1471 Fragments arranged by size: 1471 1135 1010 681 613 416 219 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5797 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5797 SmaI CCC'GGG Cuts at: 1872 3124 1872 Size: 1252 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2013 2026 3901 4802 5064 820 Size: 197 926 70 13 1875 901 262 1553 Fragments arranged by size: 1875 1553 926 901 262 197 70 13 SnaBI TAC'GTA Cuts at: 493 3588 493 Size: 3095 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5797 SphI G_CATG'C Cuts at: 1132 3155 1132 Size: 2023 3774 SspI AAT'ATT Cuts at: 5 52 3721 5 Size: 47 3669 2081 Fragments arranged by size: 3669 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5797 StyI C'CwwG_G Cuts at: 513 1065 2385 3236 513 Size: 552 1320 851 3074 Fragments arranged by size: 3074 1320 851 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2325 2398 2927 3824 3841 3994 4179 Size: 1255 745 73 529 897 17 153 185 Cuts at: 4179 4624 325 Size: 445 1498 Fragments arranged by size: 1498 1255 897 745 529 445 185 153 73 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 4043 5755 Size: 274 80 33 51 1443 792 1274 1712 Cuts at: 5755 96 Size: 138 Fragments arranged by size: 1712 1443 1274 792 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2383 2401 2415 2433 2437 2469 5757 5789 2383 Size: 18 14 18 4 32 3288 32 2391 Fragments arranged by size: 3288 2391 32 32 18 18 14 4 Tth111I GACn'n_nGTC Cuts at: 1316 2806 1316 Size: 1490 4307 XbaI T'CTAG_A Cuts at: 2851 3133 2851 Size: 282 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5797 XmaI C'CCGG_G Cuts at: 1870 3122 1870 Size: 1252 4545 XmnI GAAnn'nnTTC Cuts at: 3926 3926 Size: 5797 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3605 276 Size: 53 83 186 936 2071 2468 Fragments arranged by size: 2468 2071 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI