Restriction map for
pFN21AE0654
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspLU11I BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstBI
BstUI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoT22I FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MflI MluI MroI
MspI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 646 GTATAC GT^[AC][GT]AC
AcsI 725 AAATTC [GA]^AATT[CT]
AlwI 136 GATCC GGATC(4/5)
ApoI 725 AAATTC [GA]^AATT[CT]
AspI 1038 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 404 CCCGAG C^[CT]CG[GA]G
BciVI 120 GGATAC GTATCC(6/5)
BsaJI 1054 CCATGG C^C[ACGT][ACGT]GG
BsmAI 454 GTCTC GTCTC(1/5)
BsmFI 1062 GGGAC GGGAC(10/14)
BsoBI 404 CCCGAG C^[CT]CG[GA]G
BspHI 159 TCATGA T^CATGA
BsrBI 560 CCGCTC CCGCTC(-3/-3)
BssKI 1008 CCAGG ^CC[ACGT]GG
Bst1107I 646 GTATAC GTA^TAC
BstEII 34 GGTAACC G^GT[ACGT]ACC
BstNI 1008 CCAGG CC^[AT]GG
BstPI 34 GGTAACC G^GT[ACGT]ACC
BstZ17I 646 GTATAC GTA^TAC
DsaI 1054 CCATGG C^C[GA][CT]GG
EcoO65I 34 GGTAACC G^GT[ACGT]ACC
EcoRII 1008 CCAGG ^CC[AT]GG
EcoRV 902 GATATC GAT^ATC
EcoT14I 1054 CCATGG C^C[AT][AT]GG
FauI 559 CCCGC CCCGC(4/6)
HgaI 690 GCGTC GACGC(5/10)
MaeII 29 ACGT A^CGT
MfeI 794 CAATTG C^AATTG
MunI 794 CAATTG C^AATTG
MvaI 1008 CCAGG CC^[AT]GG
NcoI 1054 CCATGG C^CATGG
PflFI 1038 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1066 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 179 CTGCAG CTGCA^G
RcaI 159 TCATGA T^CATGA
ScrFI 1008 CCAGG CC^[ACGT]GG
SfcI 179 CTGCAG C^T[GA][CT]AG
SfiI 919 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1088 CTCAAG C^T[CT][GA]AG
StyI 1054 CCATGG C^C[AT][AT]GG
TfiI 891 GAATC G^A[AT]TC
Tsp45I 52 GTGAC ^GT[GC]AC
Tth111I 1038 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1066 CCAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 560 CCGC CCGC(-3/-1)
1084 CCGC
AvaII 91 GGACC G^G[AT]CC
1063 GGACC
BbsI 521 GAAGAC GAAGAC(2/6)
1044 GTCTTC
BfaI 251 CTAG C^TAG
626 CTAG
BglI 920 GCCAGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1086 GCCTCAAGGGC
BpuAI 521 GAAGAC GAAGAC(2/6)
1044 GTCTTC
BsgI 1049 CTGCAC GTGCAG(16/14)
782 GTGCAG
BstAPI 184 GCATTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
784 GCAGCACATGC
BstF5I 474 GGATG GGATG(2/0)
1058 GGATG
DdeI 337 CTCAG C^T[ACGT]AG
437 CTAAG
FokI 474 GGATG GGATG(9/13)
1058 GGATG
HphI 331 GGTGA GGTGA(8/7)
703 TCACC
MaeI 251 CTAG C^TAG
626 CTAG
MseI 806 TTAA T^TAA
1031 TTAA
MspA1I 125 CAGCTG C[AC]G^C[GT]G
176 CAGCTG
NspI 789 ACATGC [GA]CATG^[CT]
1106 GCATGT
PvuII 125 CAGCTG CAG^CTG
176 CAGCTG
TaqI 729 TCGA T^CGA
1134 TCGA
Tru9I 806 TTAA T^TAA
1031 TTAA
TthHB8I 729 TCGA T^CGA
1134 TCGA
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BalI BbvI BsiYI
BslI BsrI BstXI Cfr13I DpnII
EaeI Fnu4HI HaeIII HinfI ItaI
MaeIII MboI MboII MluNI MnlI
MscI MslI MwoI NdeI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I SfaNI TseI Tsp509I TspRI
XcmI