(Circular) (Six-base) MAPSORT of: pFN21AE0652.seq Check: 2355 from: 1 to: 5860 pFN21AE0652 5860 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3670 278 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2073 2145 2201 2595 3923 5307 5575 1632 Size: 441 72 56 394 1328 1384 268 1917 Fragments arranged by size: 1917 1384 1328 441 394 268 72 56 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2808 2810 4759 5736 1203 Size: 1605 2 1949 977 1327 Fragments arranged by size: 1949 1605 1327 977 2 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 2617 4589 2617 Size: 1972 3888 AleI CACnn'nnGTG Cuts at: 1166 3131 1166 Size: 1965 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5860 AlwNI CAG_nnn'CTG Cuts at: 3603 5175 3603 Size: 1572 4288 ApaLI G'TGCA_C Cuts at: 3143 3917 5073 3143 Size: 774 1156 3930 Fragments arranged by size: 3930 1156 774 ApoI r'AATT_y Cuts at: 1493 3169 3395 3547 5656 1493 Size: 1676 226 152 2109 1697 Fragments arranged by size: 2109 1697 1676 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3185 1870 Size: 73 67 1175 4545 Fragments arranged by size: 4545 1175 73 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2797 2830 2797 Size: 33 5827 BamHI G'GATC_C Cuts at: 3190 3557 3190 Size: 367 5493 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2829 3049 3181 4636 618 Size: 325 217 627 1042 220 132 1455 1842 Fragments arranged by size: 1842 1455 1042 627 325 220 217 132 BanII G_rGCy'C Cuts at: 1391 3179 1391 Size: 1788 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2061 2265 928 Size: 971 162 204 4523 Fragments arranged by size: 4523 971 204 162 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2807 2841 2991 3025 4051 4085 1903 Size: 34 870 34 150 34 1026 34 3678 Fragments arranged by size: 3678 1026 870 150 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2174 3752 4962 2174 Size: 1578 1210 3072 Fragments arranged by size: 3072 1578 1210 BclI T'GATC_A Cuts at: 1548 2490 1548 Size: 942 4918 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2067 5855 2067 Size: 3788 2072 BlpI GC'TnA_GC Cuts at: 3277 3277 Size: 5860 Bme1580I G_kGCm'C Cuts at: 1790 3147 3921 5077 1790 Size: 1357 774 1156 2573 Fragments arranged by size: 2573 1357 1156 774 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2746 3591 4549 Size: 416 53 83 399 24 1329 845 958 Cuts at: 4549 442 Size: 1753 Fragments arranged by size: 1753 1329 958 845 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2743 2047 Size: 696 5164 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2541 4520 1239 Size: 1302 1979 2579 Fragments arranged by size: 2579 1979 1302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2423 2690 3985 4850 5148 2423 Size: 267 1295 865 298 3135 Fragments arranged by size: 3135 1295 865 298 267 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 2427 3097 3109 4523 882 Size: 617 579 349 670 12 1414 2219 Fragments arranged by size: 2219 1414 670 617 579 349 12 BsaAI yAC'GTr Cuts at: 493 1204 2811 3651 493 Size: 711 1607 840 2702 Fragments arranged by size: 2702 1607 840 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2394 3667 4049 4684 Size: 53 83 186 936 861 1273 382 635 Cuts at: 4684 275 Size: 1451 Fragments arranged by size: 1451 1273 936 861 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2309 2328 2895 4293 4691 4965 5112 5523 2309 Size: 19 567 1398 398 274 147 411 2646 Fragments arranged by size: 2646 1398 567 411 398 274 147 19 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2330 2360 1178 Size: 30 165 30 927 30 4678 Fragments arranged by size: 4678 927 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2489 1186 Size: 837 466 4557 Fragments arranged by size: 4557 837 466 BseYI C'CCAG_C Cuts at: 5063 5559 5063 Size: 496 5364 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2946 3310 4071 5099 1540 Size: 275 186 945 364 761 1028 2301 Fragments arranged by size: 2301 1028 945 761 364 275 186 BsiHKAI G_wGCw'C Cuts at: 3147 3921 4006 5077 3147 Size: 774 85 1071 3930 Fragments arranged by size: 3930 1071 774 85 BsmI GAATG_Cn' Cuts at: 3371 3464 3371 Size: 93 5767 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3147 3179 3921 4006 5077 1391 Size: 399 1357 32 742 85 1071 2174 Fragments arranged by size: 2174 1357 1071 742 399 85 32 BspEI T'CCGG_A Cuts at: 2895 5523 2895 Size: 2628 3232 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3215 844 Size: 2371 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3112 3747 1394 Size: 429 1289 635 3507 Fragments arranged by size: 3507 1289 635 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4504 4691 1700 Size: 110 2694 187 2869 Fragments arranged by size: 2869 2694 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 2873 3920 4932 2873 Size: 1047 1012 3801 Fragments arranged by size: 3801 1047 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2936 794 Size: 2142 3718 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2067 2700 2892 3190 3557 3942 3959 5411 Size: 95 633 192 298 367 385 17 1452 Cuts at: 5411 5497 5509 5855 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 633 385 367 346 298 192 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2834 2940 513 Size: 552 939 830 106 3433 Fragments arranged by size: 3433 939 830 552 106 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5121 Fragments arranged by size: 5121 683 56 BtsI GCAGTG_nn' Cuts at: 2541 3147 3376 4170 4190 2541 Size: 606 229 794 20 4211 Fragments arranged by size: 4211 794 606 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4867 809 Size: 507 3551 1802 Fragments arranged by size: 3551 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2943 3027 3307 Size: 54 1425 50 275 18 1113 84 280 Cuts at: 3307 4196 5825 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1113 889 280 275 84 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2943 3307 1537 Size: 275 1131 364 4090 Fragments arranged by size: 4090 1131 364 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 3790 1353 Size: 768 1669 3423 Fragments arranged by size: 3423 1669 768 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2369 2726 4447 4821 4967 387 Size: 1064 138 780 357 1721 374 146 1280 Fragments arranged by size: 1721 1280 1064 780 374 357 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2145 2201 2541 2595 3923 4520 Size: 393 441 72 56 340 54 1328 597 Cuts at: 4520 5307 5575 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1328 787 597 441 393 340 268 72 56 54 EcoO109I rG'GnC_Cy Cuts at: 2100 2791 2100 Size: 691 5169 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2086 2086 Size: 5860 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2180 2889 5007 1678 Size: 502 709 2118 2531 Fragments arranged by size: 2531 2118 709 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2462 2494 2522 2554 2603 Size: 32 135 32 890 32 28 32 49 Cuts at: 2603 2635 2732 2764 4501 4533 4575 4607 1373 Size: 32 97 32 1737 32 42 32 2626 Fragments arranged by size: 2626 1737 890 135 97 49 42 32 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2611 3204 3450 669 Size: 988 281 673 593 246 3079 Fragments arranged by size: 3079 988 673 593 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2775 3082 4974 5158 1305 Size: 1470 307 1892 184 2007 Fragments arranged by size: 2007 1892 1470 307 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3029 10 Size: 54 1425 343 1197 2841 Fragments arranged by size: 2841 1425 1197 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2627 3131 3818 4177 4336 518 Size: 648 1461 504 687 359 159 2042 Fragments arranged by size: 2042 1461 687 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2302 3276 3953 5101 5346 1606 Size: 696 974 677 1148 245 2120 Fragments arranged by size: 2120 1148 974 696 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2834 513 Size: 552 939 830 3539 Fragments arranged by size: 3539 939 830 552 NdeI CA'TA_TG Cuts at: 387 2958 387 Size: 2571 3289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 3218 4763 1132 Size: 97 1989 1545 2229 Fragments arranged by size: 2229 1989 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 4759 4759 Size: 5860 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 2416 1108 Size: 1281 27 4552 Fragments arranged by size: 4552 1281 27 PfoI T'CCnGG_A Cuts at: 2036 2036 Size: 5860 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PmlI CAC'GTG Cuts at: 2811 2811 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3929 3961 5442 5474 3929 Size: 32 1481 32 4315 Fragments arranged by size: 4315 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2791 2791 Size: 5860 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PstI C_TGCA'G Cuts at: 830 3212 830 Size: 2382 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2302 2302 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2461 3064 3208 4343 5024 5215 826 Size: 219 1416 603 144 1135 681 191 1471 Fragments arranged by size: 1471 1416 1135 681 603 219 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 2012 3187 1872 Size: 140 1175 4545 Fragments arranged by size: 4545 1175 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 2438 2705 3964 4865 5127 820 Size: 197 926 495 267 1259 901 262 1553 Fragments arranged by size: 1553 1259 926 901 495 267 262 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 3218 1132 Size: 2086 3774 SspI AAT'ATT Cuts at: 5 52 2245 3784 5 Size: 47 2193 1539 2081 Fragments arranged by size: 2193 2081 1539 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 2834 3299 513 Size: 552 939 830 465 3074 Fragments arranged by size: 3074 939 830 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2847 3887 3904 4057 4242 4687 325 Size: 1255 1267 1040 17 153 185 445 1498 Fragments arranged by size: 1498 1267 1255 1040 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2286 2400 2457 Size: 274 80 33 51 1443 309 114 57 Cuts at: 2457 4106 5818 96 Size: 1649 1712 138 Fragments arranged by size: 1712 1649 1443 309 274 138 114 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2830 2862 5820 5852 2830 Size: 32 2958 32 2838 Fragments arranged by size: 2958 2838 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 2010 3185 1870 Size: 140 1175 4545 Fragments arranged by size: 4545 1175 140 XmnI GAAnn'nnTTC Cuts at: 2069 3989 2069 Size: 1920 3940 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3668 276 Size: 53 83 186 936 2134 2468 Fragments arranged by size: 2468 2134 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI