(Circular) (Six-base) MAPSORT of: pFN21AE0650.seq Check: 3979 from: 1 to: 5851 pFN21AE0650 5851 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2137 2137 Size: 5851 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3661 278 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2259 3036 3194 1937 Size: 322 777 158 4594 Fragments arranged by size: 4594 777 322 158 Acc65I G'GTAC_C Cuts at: 3172 3172 Size: 5851 AclI AA'CG_TT Cuts at: 1563 3978 4351 1563 Size: 2415 373 3063 Fragments arranged by size: 3063 2415 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2240 3086 3914 5298 5566 1632 Size: 608 846 828 1384 268 1917 Fragments arranged by size: 1917 1384 846 828 608 268 AfeI AGC'GCT Cuts at: 2339 2339 Size: 5851 AflII C'TTAA_G Cuts at: 820 1017 2552 820 Size: 197 1535 4119 Fragments arranged by size: 4119 1535 197 AflIII A'CryG_T Cuts at: 1203 2676 4750 5727 1203 Size: 1473 2074 977 1327 Fragments arranged by size: 2074 1473 1327 977 AgeI A'CCGG_T Cuts at: 4682 4682 Size: 5851 AhdI GACnn_n'nnGTC Cuts at: 2680 4580 2680 Size: 1900 3951 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5851 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5851 AlwNI CAG_nnn'CTG Cuts at: 2152 2473 3594 5166 2152 Size: 321 1121 1572 2837 Fragments arranged by size: 2837 1572 1121 321 ApaI G_GGCC'C Cuts at: 2039 2039 Size: 5851 ApaLI G'TGCA_C Cuts at: 3908 5064 3908 Size: 1156 4695 ApoI r'AATT_y Cuts at: 1493 3160 3386 3538 5647 1493 Size: 1667 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 226 152 AseI AT'TA_AT Cuts at: 160 1032 4405 160 Size: 872 3373 1606 Fragments arranged by size: 3373 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 AvaI C'yCGr_G Cuts at: 1870 1943 2758 2958 3176 1870 Size: 73 815 200 218 4545 Fragments arranged by size: 4545 815 218 200 73 BamHI G'GATC_C Cuts at: 3181 3548 3181 Size: 367 5484 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3172 4627 618 Size: 325 217 627 1385 1455 1842 Fragments arranged by size: 1842 1455 1385 627 325 217 BanII G_rGCy'C Cuts at: 1391 2039 2106 3170 1391 Size: 648 67 1064 4072 Fragments arranged by size: 4072 1064 648 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2867 3074 928 Size: 971 968 207 3705 Fragments arranged by size: 3705 971 968 207 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2914 2948 4042 4076 1903 Size: 34 977 34 1094 34 3678 Fragments arranged by size: 3678 1094 977 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3743 4953 3743 Size: 1210 4641 BclI T'GATC_A Cuts at: 1548 1548 Size: 5851 BfrBI ATG'CAT Cuts at: 2364 2364 Size: 5851 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2793 4462 136 Size: 107 122 71 631 605 1121 1669 1525 Fragments arranged by size: 1669 1525 1121 631 605 122 107 71 BglII A'GATC_T Cuts at: 5846 5846 Size: 5851 BlpI GC'TnA_GC Cuts at: 3268 3268 Size: 5851 Bme1580I G_kGCm'C Cuts at: 1790 2039 2046 2218 3912 5068 1790 Size: 249 7 172 1694 1156 2573 Fragments arranged by size: 2573 1694 1156 249 172 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5851 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2995 3582 4540 Size: 416 53 83 399 24 1578 587 958 Cuts at: 4540 442 Size: 1753 Fragments arranged by size: 1753 1578 958 587 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5851 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2201 4511 1239 Size: 962 2310 2579 Fragments arranged by size: 2579 2310 962 Bpu10I CC'TnA_GC Cuts at: 2577 2577 Size: 5851 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3976 4841 5139 3976 Size: 865 298 4688 Fragments arranged by size: 4688 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2576 4514 882 Size: 617 1077 1938 2219 Fragments arranged by size: 2219 1938 1077 617 BsaAI yAC'GTr Cuts at: 493 1204 3642 493 Size: 711 2438 2702 Fragments arranged by size: 2702 2438 711 BsaBI GATnn'nnATC Cuts at: 5845 5845 Size: 5851 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3658 4040 4675 275 Size: 53 83 186 936 2125 382 635 1451 Fragments arranged by size: 2125 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4284 4682 4956 5103 5514 4284 Size: 398 274 147 411 4621 Fragments arranged by size: 4621 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5626 Fragments arranged by size: 5626 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2336 2785 3017 3077 3112 1186 Size: 853 297 449 232 60 35 3925 Fragments arranged by size: 3925 853 449 297 232 60 35 BseYI C'CCAG_C Cuts at: 2152 5054 5550 2152 Size: 2902 496 2453 Fragments arranged by size: 2902 2453 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2459 1163 Size: 573 723 4555 Fragments arranged by size: 4555 723 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3301 4062 5090 1540 Size: 275 186 1300 761 1028 2301 Fragments arranged by size: 2301 1300 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2086 3912 3997 5068 2086 Size: 1826 85 1071 2869 Fragments arranged by size: 2869 1826 1071 85 BsmI GAATG_Cn' Cuts at: 2366 3362 3455 2366 Size: 996 93 4762 Fragments arranged by size: 4762 996 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2046 2086 2106 2218 3170 3912 Size: 399 249 7 40 20 112 952 742 Cuts at: 3912 3997 5068 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 952 742 399 249 112 85 40 20 7 BspEI T'CCGG_A Cuts at: 5514 5514 Size: 5851 BspHI T'CATG_A Cuts at: 2898 3740 5470 2898 Size: 842 1730 3279 Fragments arranged by size: 3279 1730 842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2137 3206 844 Size: 1293 1069 3489 Fragments arranged by size: 3489 1293 1069 BsrBI CCG'CTC Cuts at: 1394 1823 3738 1394 Size: 429 1915 3507 Fragments arranged by size: 3507 1915 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 4346 4520 65 Size: 1183 1239 1859 174 1396 Fragments arranged by size: 1859 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3081 4495 4682 1700 Size: 110 1271 1414 187 2869 Fragments arranged by size: 2869 1414 1271 187 110 BsrGI T'GTAC_A Cuts at: 96 2700 96 Size: 2604 3247 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5851 BssSI C'ACGA_G Cuts at: 3911 4923 3911 Size: 1012 4839 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5851 BstBI TT'CG_AA Cuts at: 4655 4655 Size: 5851 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5851 BstXI CCAn_nnnn'nTGG Cuts at: 3019 3019 Size: 5851 BstYI r'GATC_y Cuts at: 1972 3181 3548 3933 3950 5402 5488 5500 5846 Size: 1209 367 385 17 1452 86 12 346 Cuts at: 5846 1972 Size: 1977 Fragments arranged by size: 1977 1452 1209 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2637 2637 Size: 5851 BtgI C'CryG_G Cuts at: 513 1065 2004 2329 513 Size: 552 939 325 4035 Fragments arranged by size: 4035 939 552 325 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5112 Fragments arranged by size: 5112 683 56 BtsI GCAGTG_nn' Cuts at: 2124 2457 2553 3367 4161 4181 2124 Size: 333 96 814 794 20 3794 Fragments arranged by size: 3794 814 794 333 96 20 ClaI AT'CG_AT Cuts at: 1554 2924 1554 Size: 1370 4481 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5816 DraI TTT'AAA Cuts at: 3155 3155 Size: 5851 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4858 809 Size: 507 3542 1802 Fragments arranged by size: 3542 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3021 3298 4187 Size: 54 1425 50 275 18 1191 277 889 Cuts at: 4187 5816 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1191 889 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3298 1537 Size: 275 1486 4090 Fragments arranged by size: 4090 1486 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 3781 1353 Size: 1409 1019 3423 Fragments arranged by size: 3423 1409 1019 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2079 2837 4438 4812 4958 387 Size: 1064 138 490 758 1601 374 146 1280 Fragments arranged by size: 1601 1280 1064 758 490 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2240 3086 3914 4511 5298 5566 Size: 393 569 39 846 828 597 787 268 Cuts at: 5566 1239 Size: 1524 Fragments arranged by size: 1524 846 828 787 597 569 393 268 39 EcoO109I rG'GnC_Cy Cuts at: 2035 2036 2641 3061 2035 Size: 1 605 420 4825 Fragments arranged by size: 4825 605 420 1 EcoRI G'AATT_C Cuts at: 3160 3538 3160 Size: 378 5473 FspI TGC'GCA Cuts at: 4357 4357 Size: 5851 HaeII r_GCGC'y Cuts at: 1678 2341 4998 1678 Size: 663 2657 2531 Fragments arranged by size: 2657 2531 663 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2666 2698 2875 2907 4492 Size: 32 135 32 1094 32 177 32 1585 Cuts at: 4492 4524 4566 4598 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1585 1094 177 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 3441 669 Size: 988 281 1257 246 3079 Fragments arranged by size: 3079 1257 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5851 HpaI GTT'AAC Cuts at: 3441 3441 Size: 5851 KpnI G_GTAC'C Cuts at: 3176 3176 Size: 5851 MfeI C'AATT_G Cuts at: 3450 3450 Size: 5851 MluI A'CGCG_T Cuts at: 5727 5727 Size: 5851 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2760 4965 5149 1305 Size: 1455 2205 184 2007 Fragments arranged by size: 2205 2007 1455 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3023 10 Size: 54 1425 343 1191 2838 Fragments arranged by size: 2838 1425 1191 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2354 2499 3809 4168 4327 518 Size: 648 1188 145 1310 359 159 2042 Fragments arranged by size: 2042 1310 1188 648 359 159 145 MspA1I CmG'CkG Cuts at: 1606 2842 3267 3944 5092 5337 1606 Size: 1236 425 677 1148 245 2120 Fragments arranged by size: 2120 1236 1148 677 425 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2329 513 Size: 552 939 325 4035 Fragments arranged by size: 4035 939 552 325 NdeI CA'TA_TG Cuts at: 387 387 Size: 5851 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5851 NotI GC'GGCC_GC Cuts at: 3298 3298 Size: 5851 NsiI A_TGCA'T Cuts at: 2366 2366 Size: 5851 NspI r_CATG'y Cuts at: 1132 1229 2326 2680 3209 4754 1132 Size: 97 1097 354 529 1545 2229 Fragments arranged by size: 2229 1545 1097 529 354 97 PasI CC'CwG_GG Cuts at: 1348 2686 1348 Size: 1338 4513 PciI A'CATG_T Cuts at: 2676 4750 2676 Size: 2074 3777 PflMI CCAn_nnn'nTGG Cuts at: 1108 2926 1108 Size: 1818 4033 PfoI T'CCnGG_A Cuts at: 2304 2304 Size: 5851 PmeI GTTT'AAAC Cuts at: 3155 3155 Size: 5851 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3920 3952 5433 5465 3920 Size: 32 1481 32 4306 Fragments arranged by size: 4306 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2641 3061 2641 Size: 420 5431 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5851 PsiI TTA'TAA Cuts at: 3421 3421 Size: 5851 PspOMI G'GGCC_C Cuts at: 2035 2035 Size: 5851 PstI C_TGCA'G Cuts at: 830 2437 2604 2970 3203 830 Size: 1607 167 366 233 3478 Fragments arranged by size: 3478 1607 366 233 167 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5851 PvuII CAG'CTG Cuts at: 2842 2842 Size: 5851 SalI G'TCGA_C Cuts at: 1936 3193 1936 Size: 1257 4594 SbfI CC_TGCA'GG Cuts at: 3203 3203 Size: 5851 ScaI AGT'ACT Cuts at: 4099 5811 4099 Size: 1712 4139 SexAI A'CCwGG_T Cuts at: 3045 3147 3045 Size: 102 5749 SfcI C'TryA_G Cuts at: 826 1045 2433 2600 2966 3199 4334 5015 5206 Size: 219 1388 167 366 233 1135 681 191 Cuts at: 5206 826 Size: 1471 Fragments arranged by size: 1471 1388 1135 681 366 233 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5851 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5851 SmaI CCC'GGG Cuts at: 1872 2760 3178 1872 Size: 888 418 4545 Fragments arranged by size: 4545 888 418 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3955 4856 5118 820 Size: 197 926 609 1403 901 262 1553 Fragments arranged by size: 1553 1403 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 3642 493 Size: 3149 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5851 SphI G_CATG'C Cuts at: 1132 3209 1132 Size: 2077 3774 SspI AAT'ATT Cuts at: 5 52 3775 5 Size: 47 3723 2081 Fragments arranged by size: 3723 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5851 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2329 3018 3290 513 Size: 552 939 237 88 689 272 3074 Fragments arranged by size: 3074 939 689 552 272 237 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2138 2150 2603 2678 3878 3895 4048 Size: 1255 558 12 453 75 1200 17 153 Cuts at: 4048 4233 4678 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1200 558 453 445 185 153 75 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 4097 5809 Size: 274 80 33 51 1443 723 1397 1712 Cuts at: 5809 96 Size: 138 Fragments arranged by size: 1712 1443 1397 723 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5811 5843 5811 Size: 32 5819 Tth111I GACn'n_nGTC Cuts at: 1316 2993 1316 Size: 1677 4174 XbaI T'CTAG_A Cuts at: 3187 3187 Size: 5851 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2198 2149 Size: 49 5802 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5851 XmaI C'CCGG_G Cuts at: 1870 2758 3176 1870 Size: 888 418 4545 Fragments arranged by size: 4545 888 418 XmnI GAAnn'nnTTC Cuts at: 3980 3980 Size: 5851 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3659 276 Size: 53 83 186 936 2125 2468 Fragments arranged by size: 2468 2125 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI