Restriction map for
pFN21AE0645
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BlnI BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BsmI BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SapI SbfI SfaNI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 394 GTCGAC GT^[AC][GT]AC
Alw44I 643 GTGCAC G^TGCAC
ApaLI 643 GTGCAC G^TGCAC
BglII 434 AGATCT A^GATCT
BlpI 528 GCTCAGC GC^T[ACGT]AGC
BpmI 562 CTGGAG CTGGAG(16/14)
Bpu1102I 528 GCTCAGC GC^T[ACGT]AGC
BsaAI 631 TACGTG [CT]AC^GT[GA]
BsaI 305 GAGACC GGTCTC(1/5)
Bsp1407I 17 TGTACA T^GTACA
BsrFI 593 GCCGGT [GA]^CCGG[CT]
BsrGI 17 TGTACA T^GTACA
BstAPI 636 GCAGCCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 528 GCTCAGC GC^T[ACGT]AGC
Cfr10I 593 GCCGGT [GA]^CCGG[CT]
EaeI 206 TGGCCG [CT]^GGCC[GA]
EarI 211 GAAGAG CTCTTC(1/4)
EcoNI 357 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 566 AGCGCC [GA]GCGC^[CT]
HgaI 161 GCGTC GACGC(5/10)
HincII 394 GTCGAC GT[CT]^[GA]AC
HindII 394 GTCGAC GT[CT]^[GA]AC
HphI 469 TCACC GGTGA(8/7)
Ksp632I 211 GAAGAG CTCTTC(1/4)
MaeII 632 ACGT A^CGT
MspA1I 202 CAGCTG C[AC]G^C[GT]G
NlaIII 87 CATG CATG^
PleI 4 GAGTC GAGTC(4/5)
PvuII 202 CAGCTG CAG^CTG
SalI 394 GTCGAC G^TCGAC
ScaI 350 AGTACT AGT^ACT
SexAI 308 ACCAGGT A^CC[AT]GGT
SfcI 32 CTACAG C^T[GA][CT]AG
SspBI 17 TGTACA T^GTACA
StuI 338 AGGCCT AGG^CCT
TfiI 216 GATTC G^A[AT]TC
Tsp45I 468 GTCAC ^GT[GC]AC
TspRI 580 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 571 CCAGACCCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 78 GATCC GGATC(4/5)
252 GATCC
Asp700I 211 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
348 GAAGTACTTC
AvaI 183 CTCGGG C^[CT]CG[GA]G
342 CTCGGG
BseRI 364 GAGGAG GAGGAG(10/8)
451 GAGGAG
BsgI 313 GTGCAG GTGCAG(16/14)
634 GTGCAG
BsmAI 65 GAGAC GTCTC(1/5)
305 GAGAC
BsoBI 183 CTCGGG C^[CT]CG[GA]G
342 CTCGGG
BstYI 77 AGATCC [GA]^GATC[CT]
434 AGATCT
DdeI 507 CTCAG C^T[ACGT]AG
529 CTCAG
FauI 588 GCGGG CCCGC(4/6)
602 GCGGG
HinfI 4 GAGTC G^A[ACGT]TC
216 GATTC
MaeIII 468 GTCAC ^GT[ACGT]AC
629 GTTAC
MflI 77 AGATCC [GA]^GATC[CT]
434 AGATCT
PpuMI 515 AGGACCC [GA]G^G[AT]CC[CT]
557 AGGTCCT
SacI 118 GAGCTC GAGCT^C
267 GAGCTC
SmlI 73 CTCAAG C^T[CT][GA]AG
463 CTCAAG
TaqI 131 TCGA T^CGA
395 TCGA
TthHB8I 131 TCGA T^CGA
395 TCGA
XhoII 77 AGATCC [GA]^GATC[CT]
434 AGATCT
XmnI 211 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
348 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DraII
DsaI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
ItaI MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI