Restriction map for
pFN21AE0645
(Linear) (Six-base) MAPSORT of: KIEE0645.seq Check: 3651 from: 1 to: 651
KIEE0645.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 395 651
Size: 395 256
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 355 387 651
Size: 355 32 264
Fragments arranged by size:
355 264 32
ApaLI G'TGCA_C
Cuts at: 0 643 651
Size: 643 8
AvaI C'yCGr_G
Cuts at: 0 183 342 651
Size: 183 159 309
Fragments arranged by size:
309 183 159
BanII G_rGCy'C
Cuts at: 0 122 271 458 651
Size: 122 149 187 193
Fragments arranged by size:
193 187 149 122
BglII A'GATC_T
Cuts at: 0 434 651
Size: 434 217
BlpI GC'TnA_GC
Cuts at: 0 529 651
Size: 529 122
Bme1580I G_kGCm'C
Cuts at: 0 647 651
Size: 647 4
BmrI ACTGGGnnnn_n'
Cuts at: 0 489 651
Size: 489 162
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 88 651
Size: 88 563
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 651
Size: 583 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 448 651
Size: 58 390 203
Fragments arranged by size:
390 203 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 299 651
Size: 299 352
BsaAI yAC'GTr
Cuts at: 0 633 651
Size: 633 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 296 326 355 385 651
Size: 296 30 29 30 266
Fragments arranged by size:
296 266 30 30 29
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 466 651
Size: 379 87 185
Fragments arranged by size:
379 185 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 651
Size: 334 317
BsiHKAI G_wGCw'C
Cuts at: 0 122 271 647 651
Size: 122 149 376 4
Fragments arranged by size:
376 149 122 4
Bsp1286I G_dGCh'C
Cuts at: 0 122 271 458 647 651
Size: 122 149 187 189 4
Fragments arranged by size:
189 187 149 122 4
BsrFI r'CCGG_y
Cuts at: 0 593 651
Size: 593 58
BsrGI T'GTAC_A
Cuts at: 0 17 651
Size: 17 634
BstAPI GCAn_nnn'nTGC
Cuts at: 0 642 651
Size: 642 9
BstYI r'GATC_y
Cuts at: 0 77 434 651
Size: 77 357 217
Fragments arranged by size:
357 217 77
BtgI C'CryG_G
Cuts at: 0 8 175 540 622 651
Size: 8 167 365 82 29
Fragments arranged by size:
365 167 82 29 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 402 651
Size: 402 249
EaeI y'GGCC_r
Cuts at: 0 206 651
Size: 206 445
EarI CTCTTCn'nnn_
Cuts at: 0 206 651
Size: 206 445
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 651
Size: 526 125
EcoICRI GAG'CTC
Cuts at: 0 120 269 651
Size: 120 149 382
Fragments arranged by size:
382 149 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 583 651
Size: 583 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 651
Size: 361 290
EcoO109I rG'GnC_Cy
Cuts at: 0 476 516 558 651
Size: 476 40 42 93
Fragments arranged by size:
476 93 42 40
HaeII r_GCGC'y
Cuts at: 0 570 651
Size: 570 81
HincII GTy'rAC
Cuts at: 0 396 651
Size: 396 255
MspA1I CmG'CkG
Cuts at: 0 204 651
Size: 204 447
PasI CC'CwG_GG
Cuts at: 0 274 651
Size: 274 377
PfoI T'CCnGG_A
Cuts at: 0 254 560 651
Size: 254 306 91
Fragments arranged by size:
306 254 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 355 387 651
Size: 355 32 264
Fragments arranged by size:
355 264 32
PpuMI rG'GwC_Cy
Cuts at: 0 516 558 651
Size: 516 42 93
Fragments arranged by size:
516 93 42
PvuII CAG'CTG
Cuts at: 0 204 651
Size: 204 447
SacI G_AGCT'C
Cuts at: 0 122 271 651
Size: 122 149 380
Fragments arranged by size:
380 149 122
SalI G'TCGA_C
Cuts at: 0 394 651
Size: 394 257
ScaI AGT'ACT
Cuts at: 0 352 651
Size: 352 299
SexAI A'CCwGG_T
Cuts at: 0 308 651
Size: 308 343
SfcI C'TryA_G
Cuts at: 0 32 651
Size: 32 619
SmlI C'TyrA_G
Cuts at: 0 73 463 651
Size: 73 390 188
Fragments arranged by size:
390 188 73
StuI AGG'CCT
Cuts at: 0 340 651
Size: 340 311
TatI w'GTAC_w
Cuts at: 0 17 350 651
Size: 17 333 301
Fragments arranged by size:
333 301 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 62 94 296 328 651
Size: 62 32 202 32 323
Fragments arranged by size:
323 202 62 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 578 651
Size: 578 73
XmnI GAAnn'nnTTC
Cuts at: 0 215 352 651
Size: 215 137 299
Fragments arranged by size:
299 215 137
Enzymes that do cut:
AccI AloI ApaLI AvaI BanII BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaXI BseRI
BsgI BsiHKAI Bsp1286I BsrFI BsrGI BstAPI BstYI BtgI
BtgZI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
HaeII HincII MspA1I PasI PfoI PpiI PpuMI PvuII
SacI SalI ScaI SexAI SfcI SmlI StuI TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BmgBI BmtI
Bpu10I BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TaqII Tth111I XbaI XhoI XmaI
ZraI