Restriction map for
pFN21AE0630
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiHKAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco47III EcoO65I EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI Ksp632I MamI MfeI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 66 AACGTT AA^CGTT
AcsI 130 GAATTC [GA]^AATT[CT]
AflIII 240 ACGCGT A^C[GA][CT]GT
AlwI 408 GATCC GGATC(4/5)
AlwNI 481 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 581 CCTCAGG CC^T[ACGT]AGG
ApaI 1034 GGGCCC GGGCC^C
ApoI 130 GAATTC [GA]^AATT[CT]
AscI 267 GGCGCGCC GG^CGCGCC
AviII 113 TGCGCA TGC^GCA
AvrII 687 CCTAGG C^CTAGG
BfaI 688 CTAG C^TAG
BglI 861 GCCGGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 193 AGATCT A^GATCT
BlnI 687 CCTAGG C^CTAGG
BsaI 160 GAGACC GGTCTC(1/5)
BsmBI 908 GAGACG CGTCTC(1/5)
BspMI 815 GCAGGT ACCTGC(4/8)
BsrFI 611 ACCGGC [GA]^CCGG[CT]
BssSI 252 CACGAG CACGAG(-5/-1)
BstYI 193 AGATCT [GA]^GATC[CT]
Bsu36I 581 CCTCAGG CC^T[ACGT]AGG
Cfr10I 611 ACCGGC [GA]^CCGG[CT]
EaeI 859 CGGCCG [CT]^GGCC[GA]
EagI 859 CGGCCG C^GGCCG
EclXI 859 CGGCCG C^GGCCG
Eco52I 859 CGGCCG C^GGCCG
Eco81I 581 CCTCAGG CC^T[ACGT]AGG
EcoNI 642 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 130 GAATTC G^AATTC
FspI 113 TGCGCA TGC^GCA
HgaI 239 GACGC GACGC(5/10)
HindIII 214 AAGCTT A^AGCTT
HinfI 1084 GACTC G^A[ACGT]TC
MaeI 688 CTAG C^TAG
MaeIII 1024 GTCAC ^GT[ACGT]AC
MflI 193 AGATCT [GA]^GATC[CT]
MluI 240 ACGCGT A^CGCGT
MslI 105 CACCTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 1070 CTCGAG C^TCGAG
PflMI 465 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1084 GACTC GAGTC(4/5)
Psp1406I 66 AACGTT AA^CGTT
PvuII 277 CAGCTG CAG^CTG
SfaNI 223 GATGC GCATC(5/9)
StuI 529 AGGCCT AGG^CCT
Tsp45I 1024 GTCAC ^GT[GC]AC
Tsp509I 131 AATT ^AATT
Van91I 465 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 461 CCATCCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1070 CTCGAG C^TCGAG
XhoII 193 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 509 GTAC GT^AC
819 GTAC
AvaII 372 GGACC G^G[AT]CC
700 GGTCC
BbsI 147 GAAGAC GAAGAC(2/6)
607 GAAGAC
BpuAI 147 GAAGAC GAAGAC(2/6)
607 GAAGAC
BsgI 1017 CTGCAC GTGCAG(16/14)
46 GTGCAG
BsiEI 825 CGACCG CG[GA][CT]^CG
859 CGGCCG
BsmAI 160 GAGAC GTCTC(1/5)
908 GAGAC
BsrBI 885 GAGCGG CCGCTC(-3/-3)
1042 GAGCGG
BssHII 268 GCGCGC G^CGCGC
769 GCGCGC
BstF5I 462 CATCC GGATG(2/0)
1002 CATCC
EcoT14I 663 CCAAGG C^C[AT][AT]GG
687 CCTAGG
FokI 462 CATCC GGATG(9/13)
1002 CATCC
HphI 405 GGTGA GGTGA(8/7)
922 GGTGA
RsaI 509 GTAC GT^AC
819 GTAC
SmaI 657 CCCGGG CCC^GGG
929 CCCGGG
StyI 663 CCAAGG C^C[AT][AT]GG
687 CCTAGG
TaqI 221 TCGA T^CGA
1071 TCGA
TthHB8I 221 TCGA T^CGA
1071 TCGA
XmaCI 657 CCCGGG C^CCGGG
929 CCCGGG
XmaI 657 CCCGGG C^CCGGG
929 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
BanI BanII BbeI BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI KasI KspI
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIV PstI SacII
Sau3AI Sau96I ScrFI SfcI SfoI
SmlI TseI TspRI