(Circular) (Six-base) MAPSORT of: pFN21AE0630.seq Check: 4905 from: 1 to: 5818 pFN21AE0630 5818 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3161 1937 Size: 1224 4594 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 2072 3945 4318 1563 Size: 509 1873 373 3063 Fragments arranged by size: 3063 1873 509 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3881 5265 5533 1632 Size: 2249 1384 268 1917 Fragments arranged by size: 2249 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 2245 4717 5694 1203 Size: 1042 2472 977 1327 Fragments arranged by size: 2472 1327 1042 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 2114 1166 Size: 948 4870 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5818 AlwNI CAG_nnn'CTG Cuts at: 2491 3561 5133 2491 Size: 1070 1572 3176 Fragments arranged by size: 3176 1572 1070 ApaI G_GGCC'C Cuts at: 3043 3043 Size: 5818 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 2135 3127 3353 3505 5614 1493 Size: 642 992 226 152 2109 1697 Fragments arranged by size: 2109 1697 992 642 226 152 AscI GG'CGCG_CC Cuts at: 2273 2273 Size: 5818 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2662 2934 3075 3143 1870 Size: 73 719 272 141 68 4545 Fragments arranged by size: 4545 719 272 141 73 68 AvrII C'CTAG_G Cuts at: 2692 2692 Size: 5818 BamHI G'GATC_C Cuts at: 3148 3515 3148 Size: 367 5451 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2580 3002 3051 3060 3139 Size: 325 217 627 793 422 49 9 79 Cuts at: 3139 4594 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 793 627 422 325 217 79 49 9 BanII G_rGCy'C Cuts at: 1391 2342 2388 3043 3137 1391 Size: 951 46 655 94 4072 Fragments arranged by size: 4072 951 655 94 46 BbeI G_GCGC'C Cuts at: 3006 3055 3064 3006 Size: 49 9 5760 Fragments arranged by size: 5760 49 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2159 2619 928 Size: 971 260 460 4127 Fragments arranged by size: 4127 971 460 260 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2872 4429 136 Size: 107 122 71 631 605 1200 1557 1525 Fragments arranged by size: 1557 1525 1200 631 605 122 107 71 BglII A'GATC_T Cuts at: 2198 5813 2198 Size: 3615 2203 BlpI GC'TnA_GC Cuts at: 3235 3235 Size: 5818 Bme1580I G_kGCm'C Cuts at: 1790 2766 2985 3043 3879 5035 1790 Size: 976 219 58 836 1156 2573 Fragments arranged by size: 2573 1156 976 836 219 58 BmgBI CAC'GTC Cuts at: 1318 2569 1318 Size: 1251 4567 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2018 2704 3549 Size: 416 53 83 399 24 601 686 845 Cuts at: 3549 4507 442 Size: 958 1753 Fragments arranged by size: 1753 958 845 686 601 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2402 2498 2669 3065 4478 1239 Size: 1163 96 171 396 1413 2579 Fragments arranged by size: 2579 1413 1163 396 171 96 Bpu10I CC'TnA_GC Cuts at: 2888 2888 Size: 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2132 2144 3943 4808 5106 2132 Size: 12 1799 865 298 2844 Fragments arranged by size: 2844 1799 865 298 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2159 4481 882 Size: 617 660 2322 2219 Fragments arranged by size: 2322 2219 660 617 BsaAI yAC'GTr Cuts at: 493 1204 3609 493 Size: 711 2405 2702 Fragments arranged by size: 2702 2405 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3003 3052 3061 3625 Size: 53 83 186 936 1470 49 9 564 Cuts at: 3625 4007 4642 275 Size: 382 635 1451 Fragments arranged by size: 1470 1451 936 635 564 382 186 83 53 49 9 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2689 2719 2953 2983 1178 Size: 30 165 30 1286 30 234 30 4013 Fragments arranged by size: 4013 1286 234 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2666 2925 1186 Size: 1258 222 259 4079 Fragments arranged by size: 4079 1258 259 222 BseYI C'CCAG_C Cuts at: 2875 5021 5517 2875 Size: 2146 496 3176 Fragments arranged by size: 3176 2146 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 3007 1163 Size: 573 336 935 3974 Fragments arranged by size: 3974 935 573 336 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2833 2867 3268 4029 5057 1540 Size: 275 186 832 34 401 761 1028 2301 Fragments arranged by size: 2301 1028 832 761 401 275 186 34 BsiHKAI G_wGCw'C Cuts at: 3879 3964 5035 3879 Size: 85 1071 4662 Fragments arranged by size: 4662 1071 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 BsmBI CGTCTCn'nnnn_ Cuts at: 2907 2907 Size: 5818 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2342 2388 2766 2985 3043 3137 3879 Size: 399 552 46 378 219 58 94 742 Cuts at: 3879 3964 5035 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 552 399 378 219 94 85 58 46 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2811 3106 3173 844 Size: 1967 295 67 3489 Fragments arranged by size: 3489 1967 295 67 BsrBI CCG'CTC Cuts at: 1394 1823 2892 3049 3705 1394 Size: 429 1069 157 656 3507 Fragments arranged by size: 3507 1069 656 429 157 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2616 4462 4649 1700 Size: 110 806 1846 187 2869 Fragments arranged by size: 2869 1846 806 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 2273 2774 1925 Size: 348 501 4969 Fragments arranged by size: 4969 501 348 BssSI C'ACGA_G Cuts at: 2257 3878 4890 2257 Size: 1621 1012 3185 Fragments arranged by size: 3185 1621 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstYI r'GATC_y Cuts at: 1972 2198 3148 3515 3900 3917 5369 5455 5467 Size: 226 950 367 385 17 1452 86 12 Cuts at: 5467 5813 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 950 385 367 346 226 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2587 2587 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2004 2263 2766 2803 2850 2905 3056 Size: 552 939 259 503 37 47 55 151 Cuts at: 3056 513 Size: 3275 Fragments arranged by size: 3275 939 552 503 259 151 55 47 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5079 Fragments arranged by size: 5079 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5783 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2864 3265 4154 Size: 54 1425 50 275 18 1034 401 889 Cuts at: 4154 5783 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1034 889 401 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2864 3265 1537 Size: 275 1052 401 4090 Fragments arranged by size: 4090 1052 401 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3748 1353 Size: 2395 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4405 4779 4925 387 Size: 1064 138 2816 374 146 1280 Fragments arranged by size: 2816 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2402 2498 2669 3065 3881 4478 5265 Size: 393 770 96 171 396 816 597 787 Cuts at: 5265 5533 1239 Size: 268 1524 Fragments arranged by size: 1524 816 787 770 597 396 393 268 171 96 EcoNI CCTnn'n_nnAGG Cuts at: 2651 2651 Size: 5818 EcoO109I rG'GnC_Cy Cuts at: 2012 3039 3040 2012 Size: 1027 1 4790 Fragments arranged by size: 4790 1027 1 EcoRI G'AATT_C Cuts at: 2135 3127 3505 2135 Size: 992 378 4448 Fragments arranged by size: 4448 992 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2185 2185 Size: 5818 FspI TGC'GCA Cuts at: 2120 4324 2120 Size: 2204 3614 HaeII r_GCGC'y Cuts at: 1678 2642 2883 3006 3055 3064 4965 1678 Size: 964 241 123 49 9 1901 2531 Fragments arranged by size: 2531 1901 964 241 123 49 9 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2689 2721 2741 2773 4459 Size: 32 135 32 1117 32 20 32 1686 Cuts at: 4459 4491 4533 4565 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1686 1117 135 42 32 32 32 32 32 32 20 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 2219 748 Size: 1471 4347 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KasI G'GCGC_C Cuts at: 3002 3051 3060 3002 Size: 49 9 5760 Fragments arranged by size: 5760 49 9 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 2245 5694 2245 Size: 3449 2369 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2268 2769 4932 5116 1305 Size: 963 501 2163 184 2007 Fragments arranged by size: 2163 2007 963 501 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3996 Fragments arranged by size: 3996 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2114 3776 4135 4294 518 Size: 648 948 1662 359 159 2042 Fragments arranged by size: 2042 1662 948 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2265 2284 2703 2768 2805 2852 3018 3234 Size: 659 19 419 65 37 47 166 216 Cuts at: 3234 3911 5059 5304 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 659 419 245 216 166 65 47 37 19 NarI GG'CG_CC Cuts at: 3003 3052 3061 3003 Size: 49 9 5760 Fragments arranged by size: 5760 49 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4327 Fragments arranged by size: 4327 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5818 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 3176 4721 1132 Size: 97 1947 1545 2229 Fragments arranged by size: 2229 1947 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5818 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2476 1108 Size: 1368 4450 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PspOMI G'GGCC_C Cuts at: 3039 3039 Size: 5818 PspXI vC'TCGA_Gb Cuts at: 3075 3075 Size: 5818 PstI C_TGCA'G Cuts at: 830 2283 2323 2505 3170 830 Size: 1453 40 182 665 3478 Fragments arranged by size: 3478 1453 665 182 40 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 PvuII CAG'CTG Cuts at: 2284 2284 Size: 5818 SacII CC_GC'GG Cuts at: 2266 2769 2806 2853 2266 Size: 503 37 47 5231 Fragments arranged by size: 5231 503 47 37 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SbfI CC_TGCA'GG Cuts at: 3170 3170 Size: 5818 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SfcI C'TryA_G Cuts at: 826 1045 2279 2319 2501 3166 4301 4982 5173 Size: 219 1234 40 182 665 1135 681 191 Cuts at: 5173 826 Size: 1471 Fragments arranged by size: 1471 1234 1135 681 665 219 191 182 40 SfoI GGC'GCC Cuts at: 3004 3053 3062 3004 Size: 49 9 5760 Fragments arranged by size: 5760 49 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5818 SmaI CCC'GGG Cuts at: 1872 2664 2936 3145 1872 Size: 792 272 209 4545 Fragments arranged by size: 4545 792 272 209 SmlI C'TyrA_G Cuts at: 820 1017 1943 2147 2159 3075 3922 4823 5085 Size: 197 926 204 12 916 847 901 262 Cuts at: 5085 820 Size: 1553 Fragments arranged by size: 1553 926 916 901 847 262 204 197 12 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 2536 1517 Size: 1019 4799 StyI C'CwwG_G Cuts at: 513 1065 2004 2668 2692 3257 513 Size: 552 939 664 24 565 3074 Fragments arranged by size: 3074 939 664 565 552 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4064 5776 96 Size: 274 80 33 51 1443 2087 1712 138 Fragments arranged by size: 2087 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2953 2985 5778 5810 2953 Size: 32 2793 32 2961 Fragments arranged by size: 2961 2793 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5818 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2473 2473 Size: 5818 XhoI C'TCGA_G Cuts at: 1943 3075 1943 Size: 1132 4686 XmaI C'CCGG_G Cuts at: 1870 2662 2934 3143 1870 Size: 792 272 209 4545 Fragments arranged by size: 4545 792 272 209 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI