Restriction map for
pFN21AE0630
(Linear) (Six-base) MAPSORT of: KIEE0630.seq Check: 8739 from: 1 to: 1113
KIEE0630.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AclI AA'CG_TT
Cuts at: 0 67 1113
Size: 67 1046
AflIII A'CryG_T
Cuts at: 0 240 1113
Size: 240 873
AleI CACnn'nnGTG
Cuts at: 0 109 1113
Size: 109 1004
AlwNI CAG_nnn'CTG
Cuts at: 0 486 1113
Size: 486 627
ApaI G_GGCC'C
Cuts at: 0 1038 1113
Size: 1038 75
ApoI r'AATT_y
Cuts at: 0 130 1113
Size: 130 983
AscI GG'CGCG_CC
Cuts at: 0 268 1113
Size: 268 845
AvaI C'yCGr_G
Cuts at: 0 657 929 1070 1113
Size: 657 272 141 43
Fragments arranged by size:
657 272 141 43
AvrII C'CTAG_G
Cuts at: 0 687 1113
Size: 687 426
BanI G'GyrC_C
Cuts at: 0 575 997 1046 1055 1113
Size: 575 422 49 9 58
Fragments arranged by size:
575 422 58 49 9
BanII G_rGCy'C
Cuts at: 0 337 383 1038 1113
Size: 337 46 655 75
Fragments arranged by size:
655 337 75 46
BbeI G_GCGC'C
Cuts at: 0 1001 1050 1059 1113
Size: 1001 49 9 54
Fragments arranged by size:
1001 54 49 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 154 614 1113
Size: 154 460 499
Fragments arranged by size:
499 460 154
BglI GCCn_nnn'nGGC
Cuts at: 0 867 1113
Size: 867 246
BglII A'GATC_T
Cuts at: 0 193 1113
Size: 193 920
Bme1580I G_kGCm'C
Cuts at: 0 761 980 1038 1113
Size: 761 219 58 75
Fragments arranged by size:
761 219 75 58
BmgBI CAC'GTC
Cuts at: 0 564 1113
Size: 564 549
BmrI ACTGGGnnnn_n'
Cuts at: 0 13 699 1113
Size: 13 686 414
Fragments arranged by size:
686 414 13
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 493 664 1060 1113
Size: 397 96 171 396 53
Fragments arranged by size:
397 396 171 96 53
Bpu10I CC'TnA_GC
Cuts at: 0 883 1113
Size: 883 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 139 1113
Size: 127 12 974
Fragments arranged by size:
974 127 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 154 1113
Size: 154 959
BsaHI Gr'CG_yC
Cuts at: 0 998 1047 1056 1113
Size: 998 49 9 57
Fragments arranged by size:
998 57 49 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 684 714 948 978 1113
Size: 684 30 234 30 135
Fragments arranged by size:
684 234 135 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 661 920 1113
Size: 439 222 259 193
Fragments arranged by size:
439 259 222 193
BseYI C'CCAG_C
Cuts at: 0 870 1113
Size: 870 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1002 1113
Size: 67 935 111
Fragments arranged by size:
935 111 67
BsiEI CG_ry'CG
Cuts at: 0 828 862 1113
Size: 828 34 251
Fragments arranged by size:
828 251 34
BsmBI CGTCTCn'nnnn_
Cuts at: 0 902 1113
Size: 902 211
Bsp1286I G_dGCh'C
Cuts at: 0 337 383 761 980 1038 1113
Size: 337 46 378 219 58 75
Fragments arranged by size:
378 337 219 75 58 46
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 806 1113
Size: 806 307
BsrBI CCG'CTC
Cuts at: 0 887 1044 1113
Size: 887 157 69
Fragments arranged by size:
887 157 69
BsrFI r'CCGG_y
Cuts at: 0 611 1113
Size: 611 502
BssHII G'CGCG_C
Cuts at: 0 268 769 1113
Size: 268 501 344
Fragments arranged by size:
501 344 268
BssSI C'ACGA_G
Cuts at: 0 252 1113
Size: 252 861
BstYI r'GATC_y
Cuts at: 0 193 1113
Size: 193 920
Bsu36I CC'TnA_GG
Cuts at: 0 582 1113
Size: 582 531
BtgI C'CryG_G
Cuts at: 0 258 761 798 845 900 1051 1113
Size: 258 503 37 47 55 151 62
Fragments arranged by size:
503 258 151 62 55 47 37
EaeI y'GGCC_r
Cuts at: 0 859 1113
Size: 859 254
EagI C'GGCC_G
Cuts at: 0 859 1113
Size: 859 254
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 493 664 1060 1113
Size: 397 96 171 396 53
Fragments arranged by size:
397 396 171 96 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 1113
Size: 646 467
EcoO109I rG'GnC_Cy
Cuts at: 0 7 1034 1035 1113
Size: 7 1027 1 78
Fragments arranged by size:
1027 78 7 1
EcoRI G'AATT_C
Cuts at: 0 130 1113
Size: 130 983
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 180 1113
Size: 180 933
FspI TGC'GCA
Cuts at: 0 115 1113
Size: 115 998
HaeII r_GCGC'y
Cuts at: 0 637 878 1001 1050 1059 1113
Size: 637 241 123 49 9 54
Fragments arranged by size:
637 241 123 54 49 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 684 716 736 768 1113
Size: 684 32 20 32 345
Fragments arranged by size:
684 345 32 32 20
HindIII A'AGCT_T
Cuts at: 0 214 1113
Size: 214 899
KasI G'GCGC_C
Cuts at: 0 997 1046 1055 1113
Size: 997 49 9 58
Fragments arranged by size:
997 58 49 9
MluI A'CGCG_T
Cuts at: 0 240 1113
Size: 240 873
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 764 1113
Size: 263 501 349
Fragments arranged by size:
501 349 263
MslI CAynn'nnrTG
Cuts at: 0 109 1113
Size: 109 1004
MspA1I CmG'CkG
Cuts at: 0 260 279 698 763 800 847 1013 1113
Size: 260 19 419 65 37 47 166 100
Fragments arranged by size:
419 260 166 100 65 47 37 19
NarI GG'CG_CC
Cuts at: 0 998 1047 1056 1113
Size: 998 49 9 57
Fragments arranged by size:
998 57 49 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 471 1113
Size: 471 642
PspOMI G'GGCC_C
Cuts at: 0 1034 1113
Size: 1034 79
PspXI vC'TCGA_Gb
Cuts at: 0 1070 1113
Size: 1070 43
PstI C_TGCA'G
Cuts at: 0 278 318 500 1113
Size: 278 40 182 613
Fragments arranged by size:
613 278 182 40
PvuII CAG'CTG
Cuts at: 0 279 1113
Size: 279 834
SacII CC_GC'GG
Cuts at: 0 261 764 801 848 1113
Size: 261 503 37 47 265
Fragments arranged by size:
503 265 261 47 37
SfcI C'TryA_G
Cuts at: 0 274 314 496 1113
Size: 274 40 182 617
Fragments arranged by size:
617 274 182 40
SfoI GGC'GCC
Cuts at: 0 999 1048 1057 1113
Size: 999 49 9 56
Fragments arranged by size:
999 56 49 9
SmaI CCC'GGG
Cuts at: 0 659 931 1113
Size: 659 272 182
Fragments arranged by size:
659 272 182
SmlI C'TyrA_G
Cuts at: 0 142 154 1070 1113
Size: 142 12 916 43
Fragments arranged by size:
916 142 43 12
StuI AGG'CCT
Cuts at: 0 531 1113
Size: 531 582
StyI C'CwwG_G
Cuts at: 0 663 687 1113
Size: 663 24 426
Fragments arranged by size:
663 426 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 948 980 1113
Size: 948 32 133
Fragments arranged by size:
948 133 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 468 1113
Size: 468 645
XhoI C'TCGA_G
Cuts at: 0 1070 1113
Size: 1070 43
XmaI C'CCGG_G
Cuts at: 0 657 929 1113
Size: 657 272 184
Fragments arranged by size:
657 272 184
Enzymes that do cut:
AclI AflIII AleI AlwNI ApaI ApoI AscI AvaI
AvrII BanI BanII BbeI BbsI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssHII BssSI BstYI Bsu36I BtgI EaeI EagI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HindIII KasI MluI MmeI MslI MspA1I NarI PflMI
PspOMI PspXI PstI PvuII SacII SfcI SfoI SmaI
SmlI StuI StyI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AgeI
AhdI AlfI AloI ApaLI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BplI
BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI BspEI BspHI
BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoRV FseI FspAI HincII HpaI KpnI MfeI
MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI Tth111I XbaI XmnI ZraI