Restriction map for
pFN21AE0624
(Linear) (Six-base) MAPSORT of: KIEE0624.seq Check: 7229 from: 1 to: 1110
KIEE0624.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1110
Size: 581 529
AleI CACnn'nnGTG
Cuts at: 0 931 1110
Size: 931 179
AlwNI CAG_nnn'CTG
Cuts at: 0 132 375 1024 1110
Size: 132 243 649 86
Fragments arranged by size:
649 243 132 86
AvaI C'yCGr_G
Cuts at: 0 510 1110
Size: 510 600
BanI G'GyrC_C
Cuts at: 0 964 1110
Size: 964 146
BanII G_rGCy'C
Cuts at: 0 308 1110
Size: 308 802
BbsI GAAGACnn'nnnn_
Cuts at: 0 467 1110
Size: 467 643
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 683 1043 1077 1110
Size: 649 34 360 34 33
Fragments arranged by size:
649 360 34 34 33
BciVI GTATCCnnnnn_n'
Cuts at: 0 541 1110
Size: 541 569
BfrBI ATG'CAT
Cuts at: 0 149 1110
Size: 149 961
BglI GCCn_nnn'nGGC
Cuts at: 0 160 1110
Size: 160 950
Bme1580I G_kGCm'C
Cuts at: 0 82 967 1078 1110
Size: 82 885 111 32
Fragments arranged by size:
885 111 82 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 732 1110
Size: 732 378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 1110
Size: 729 381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1110
Size: 187 923
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1110
Size: 409 701
BsaAI yAC'GTr
Cuts at: 0 552 1110
Size: 552 558
BsaHI Gr'CG_yC
Cuts at: 0 350 1110
Size: 350 760
BseYI C'CCAG_C
Cuts at: 0 3 960 1024 1110
Size: 3 957 64 86
Fragments arranged by size:
957 86 64 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1110
Size: 193 917
BsiHKAI G_wGCw'C
Cuts at: 0 308 524 1110
Size: 308 216 586
Fragments arranged by size:
586 308 216
Bsp1286I G_dGCh'C
Cuts at: 0 82 308 524 967 1078 1110
Size: 82 226 216 443 111 32
Fragments arranged by size:
443 226 216 111 82 32
BspHI T'CATG_A
Cuts at: 0 473 1110
Size: 473 637
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 401 1110
Size: 401 709
BsrBI CCG'CTC
Cuts at: 0 345 1110
Size: 345 765
BsrDI GCAATG_nn'
Cuts at: 0 149 1110
Size: 149 961
BsrFI r'CCGG_y
Cuts at: 0 1032 1110
Size: 1032 78
BsrGI T'GTAC_A
Cuts at: 0 411 1110
Size: 411 699
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 1110
Size: 810 300
BstEII G'GTnAC_C
Cuts at: 0 640 1110
Size: 640 470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 933 1110
Size: 933 177
BstYI r'GATC_y
Cuts at: 0 420 1110
Size: 420 690
BtgI C'CryG_G
Cuts at: 0 820 926 999 1110
Size: 820 106 73 111
Fragments arranged by size:
820 111 106 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 603 1110
Size: 603 507
BtsI GCAGTG_nn'
Cuts at: 0 17 522 1093 1110
Size: 17 505 571 17
Fragments arranged by size:
571 505 17 17
EaeI y'GGCC_r
Cuts at: 0 137 1110
Size: 137 973
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 52 1110
Size: 52 1058
EcoICRI GAG'CTC
Cuts at: 0 306 1110
Size: 306 804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 581 1110
Size: 187 394 529
Fragments arranged by size:
529 394 187
EcoO109I rG'GnC_Cy
Cuts at: 0 19 86 162 507 777 1110
Size: 19 67 76 345 270 333
Fragments arranged by size:
345 333 270 76 67 19
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 933 1110
Size: 906 27 177
Fragments arranged by size:
906 177 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 589 621 718 750 1110
Size: 448 32 109 32 97 32 360
Fragments arranged by size:
448 360 109 97 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1110
Size: 506 604
MscI TGG'CCA
Cuts at: 0 139 1110
Size: 139 971
MslI CAynn'nnrTG
Cuts at: 0 931 1110
Size: 931 179
MspA1I CmG'CkG
Cuts at: 0 288 960 1039 1110
Size: 288 672 79 71
Fragments arranged by size:
672 288 79 71
NaeI GCC'GGC
Cuts at: 0 1034 1110
Size: 1034 76
NcoI C'CATG_G
Cuts at: 0 820 999 1110
Size: 820 179 111
Fragments arranged by size:
820 179 111
NgoMIV G'CCGG_C
Cuts at: 0 1032 1110
Size: 1032 78
NsiI A_TGCA'T
Cuts at: 0 151 1110
Size: 151 959
PflMI CCAn_nnn'nTGG
Cuts at: 0 375 1024 1110
Size: 375 649 86
Fragments arranged by size:
649 375 86
PfoI T'CCnGG_A
Cuts at: 0 543 1110
Size: 543 567
PpuMI rG'GwC_Cy
Cuts at: 0 507 777 1110
Size: 507 270 333
Fragments arranged by size:
507 333 270
PstI C_TGCA'G
Cuts at: 0 959 1110
Size: 959 151
PvuII CAG'CTG
Cuts at: 0 288 960 1039 1110
Size: 288 672 79 71
Fragments arranged by size:
672 288 79 71
SacI G_AGCT'C
Cuts at: 0 308 1110
Size: 308 802
SfcI C'TryA_G
Cuts at: 0 447 582 955 1110
Size: 447 135 373 155
Fragments arranged by size:
447 373 155 135
SmaI CCC'GGG
Cuts at: 0 512 1110
Size: 512 598
SmlI C'TyrA_G
Cuts at: 0 424 1110
Size: 424 686
StuI AGG'CCT
Cuts at: 0 808 1110
Size: 808 302
StyI C'CwwG_G
Cuts at: 0 155 820 999 1110
Size: 155 665 179 111
Fragments arranged by size:
665 179 155 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 833 1110
Size: 833 277
TatI w'GTAC_w
Cuts at: 0 411 443 1110
Size: 411 32 667
Fragments arranged by size:
667 411 32
XbaI T'CTAG_A
Cuts at: 0 843 1110
Size: 843 267
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 1110
Size: 162 948
XmaI C'CCGG_G
Cuts at: 0 510 1110
Size: 510 600
XmnI GAAnn'nnTTC
Cuts at: 0 790 1110
Size: 790 320
Enzymes that do cut:
AcuI AleI AlwNI AvaI BanI BanII BbsI BcgI
BciVI BfrBI BglI Bme1580I BmrI BplI BpmI BpuEI
BsaAI BsaHI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI
BtgI BtgZI BtsI EaeI EciI EcoICRI Eco57MI EcoO109I
FalI Hin4I MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NsiI PflMI PfoI PpuMI PstI PvuII SacI
SfcI SmaI SmlI StuI StyI TaqII TatI XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BclI
BglII BlpI BmgBI BmtI Bpu10I BsaI BsaBI BsaWI
BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BssHII
BssSI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII
DrdI EagI EarI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XhoI
ZraI