Restriction map for
pFN21AE0618
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HapII
Hin1I HindIII HpaI HpaII KasI
KpnI KspI MamI MfeI MluI
MroI MseI MspI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 739 CGCG CG^CG
AcsI 146 AAATTC [GA]^AATT[CT]
AhdI 744 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 345 GATCC GGATC(4/5)
AocI 525 CCTGAGG CC^T[ACGT]AGG
ApaI 359 GGGCCC GGGCC^C
ApoI 146 AAATTC [GA]^AATT[CT]
AspEI 744 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 356 CTCGGG C^[CT]CG[GA]G
AviII 884 TGCGCA TGC^GCA
BalI 860 TGGCCA TGG^CCA
BbsI 615 GAAGAC GAAGAC(2/6)
BfaI 892 CTAG C^TAG
BglII 225 AGATCT A^GATCT
BpuAI 615 GAAGAC GAAGAC(2/6)
BsaI 542 GAGACC GGTCTC(1/5)
BsmI 532 GAATGC GAATGC(1/-1)
BsoBI 356 CTCGGG C^[CT]CG[GA]G
BstAPI 876 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 739 CGCG CG^CG
BstYI 225 AGATCT [GA]^GATC[CT]
Bsu36I 525 CCTGAGG CC^T[ACGT]AGG
DsaI 576 CCGTGG C^C[GA][CT]GG
EaeI 860 TGGCCA [CT]^GGCC[GA]
Eam1105I 744 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 280 CTCTTC CTCTTC(1/4)
Eco81I 525 CCTGAGG CC^T[ACGT]AGG
EcoT14I 1070 CCTTGG C^C[AT][AT]GG
FspI 884 TGCGCA TGC^GCA
HgaI 572 GCGTC GACGC(5/10)
HincII 442 GTTGAC GT[CT]^[GA]AC
HindII 442 GTTGAC GT[CT]^[GA]AC
Ksp632I 280 CTCTTC CTCTTC(1/4)
MaeI 892 CTAG C^TAG
MaeII 641 ACGT A^CGT
MflI 225 AGATCT [GA]^GATC[CT]
MluNI 860 TGGCCA TGG^CCA
MscI 860 TGGCCA TGG^CCA
MspA1I 364 CCGCTG C[AC]G^C[GT]G
MvnI 739 CGCG CG^CG
NspI 665 ACATGC [GA]CATG^[CT]
PstI 1081 CTGCAG CTGCA^G
SfaNI 1036 GCATC GCATC(5/9)
SfcI 1081 CTGCAG C^T[GA][CT]AG
StyI 1070 CCTTGG C^C[AT][AT]GG
TaqI 981 TCGA T^CGA
Tsp509I 147 AATT ^AATT
TthHB8I 981 TCGA T^CGA
XhoII 225 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 153 GTAC GT^AC
762 GTAC
BseRI 64 GAGGAG GAGGAG(10/8)
700 GAGGAG
BsgI 655 CTGCAC GTGCAG(16/14)
874 GTGCAG
BsrI 126 ACTGG ACTGG(1/-1)
1003 ACTGG
CfoI 738 GCGC GCG^C
885 GCGC
DpnII 226 GATC ^GATC
345 GATC
HhaI 738 GCGC GCG^C
885 GCGC
HinP1I 738 GCGC G^CGC
885 GCGC
MboI 226 GATC ^GATC
345 GATC
MslI 729 CACCGTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
867 CATCCCTGTG
NdeII 226 GATC ^GATC
345 GATC
PleI 744 GACTC GAGTC(4/5)
927 GACTC
PpuMI 331 AGGACCC [GA]G^G[AT]CC[CT]
505 GGGTCCT
RsaI 153 GTAC GT^AC
762 GTAC
Sau3AI 226 GATC ^GATC
345 GATC
TfiI 83 GATTC G^A[AT]TC
983 GAATC
XcmI 261 CCAGGAGCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
567 CCATAGCGTCCGTGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwNI AvaII BanII
BbvI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EcoNI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI Tsp45I TspRI