(Circular) (Six-base) MAPSORT of: pFN21AE0618.seq Check: 2661 from: 1 to: 5803 pFN21AE0618 5803 bp With 182 enzymes: SgfI * August 20, 2012 11:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3613 278 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3146 1937 Size: 1209 4594 Acc65I G'GTAC_C Cuts at: 3124 3124 Size: 5803 AclI AA'CG_TT Cuts at: 1563 3930 4303 1563 Size: 2367 373 3063 Fragments arranged by size: 3063 2367 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3866 5250 5518 1632 Size: 2234 1384 268 1917 Fragments arranged by size: 2234 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5606 AflIII A'CryG_T Cuts at: 1203 4702 5679 1203 Size: 3499 977 1327 Fragments arranged by size: 3499 1327 977 AgeI A'CCGG_T Cuts at: 4634 4634 Size: 5803 AhdI GACnn_n'nnGTC Cuts at: 2754 4532 2754 Size: 1778 4025 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5803 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3111 3114 1184 Size: 1927 3 3873 Fragments arranged by size: 3873 1927 3 AlwNI CAG_nnn'CTG Cuts at: 2272 2680 2887 3546 5118 2272 Size: 408 207 659 1572 2957 Fragments arranged by size: 2957 1572 659 408 207 ApaI G_GGCC'C Cuts at: 2368 2368 Size: 5803 ApaLI G'TGCA_C Cuts at: 3860 5016 3860 Size: 1156 4647 ApoI r'AATT_y Cuts at: 1493 2151 3112 3338 3490 5599 1493 Size: 658 961 226 152 2109 1697 Fragments arranged by size: 2109 1697 961 658 226 152 AseI AT'TA_AT Cuts at: 160 1032 4357 160 Size: 872 3325 1606 Fragments arranged by size: 3325 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 AvaI C'yCGr_G Cuts at: 1870 1943 2361 3128 1870 Size: 73 418 767 4545 Fragments arranged by size: 4545 767 418 73 BamHI G'GATC_C Cuts at: 3133 3500 3133 Size: 367 5436 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3124 4579 618 Size: 325 217 627 1337 1455 1842 Fragments arranged by size: 1842 1455 1337 627 325 217 BanII G_rGCy'C Cuts at: 1391 2240 2368 2732 3122 1391 Size: 849 128 364 390 4072 Fragments arranged by size: 4072 849 390 364 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2627 928 Size: 971 728 4104 Fragments arranged by size: 4104 971 728 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3994 4028 1903 Size: 34 2057 34 3678 Fragments arranged by size: 3678 2057 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3695 4905 3695 Size: 1210 4593 BclI T'GATC_A Cuts at: 1548 1548 Size: 5803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4414 136 Size: 107 122 71 631 605 2742 1525 Fragments arranged by size: 2742 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2230 5798 2230 Size: 3568 2235 BlpI GC'TnA_GC Cuts at: 3220 3220 Size: 5803 Bme1580I G_kGCm'C Cuts at: 1790 2368 3864 5020 1790 Size: 578 1496 1156 2573 Fragments arranged by size: 2573 1496 1156 578 BmgBI CAC'GTC Cuts at: 1318 2647 1318 Size: 1329 4474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3534 4492 442 Size: 416 53 83 399 24 2117 958 1753 Fragments arranged by size: 2117 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5803 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2067 2744 2969 4463 1239 Size: 828 677 225 1494 2579 Fragments arranged by size: 2579 1494 828 677 225 Bpu10I CC'TnA_GC Cuts at: 2552 2697 2552 Size: 145 5658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3928 4793 5091 3928 Size: 865 298 4640 Fragments arranged by size: 4640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2541 4466 882 Size: 617 1042 1925 2219 Fragments arranged by size: 2219 1925 1042 617 BsaAI yAC'GTr Cuts at: 493 1204 3594 493 Size: 711 2390 2702 Fragments arranged by size: 2702 2390 711 BsaBI GATnn'nnATC Cuts at: 5797 5797 Size: 5803 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3610 3992 4627 275 Size: 53 83 186 936 2077 382 635 1451 Fragments arranged by size: 2077 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4236 4634 4908 5055 5466 4236 Size: 398 274 147 411 4573 Fragments arranged by size: 4573 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5578 Fragments arranged by size: 5578 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2720 1186 Size: 898 636 4269 Fragments arranged by size: 4269 898 636 BseYI C'CCAG_C Cuts at: 5006 5502 5006 Size: 496 5307 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2645 2900 1163 Size: 573 909 255 4066 Fragments arranged by size: 4066 909 573 255 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3253 4014 5042 1540 Size: 275 186 1252 761 1028 2301 Fragments arranged by size: 2301 1252 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3864 3949 5020 3864 Size: 85 1071 4647 Fragments arranged by size: 4647 1071 85 BsmI GAATG_Cn' Cuts at: 2543 3314 3407 2543 Size: 771 93 4939 Fragments arranged by size: 4939 771 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2240 2368 2732 3122 3864 3949 5020 Size: 399 450 128 364 390 742 85 1071 Cuts at: 5020 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 450 399 390 364 128 85 BspEI T'CCGG_A Cuts at: 5466 5466 Size: 5803 BspHI T'CATG_A Cuts at: 3692 5422 3692 Size: 1730 4073 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3158 844 Size: 2314 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3690 1394 Size: 429 1867 3507 Fragments arranged by size: 3507 1867 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4298 4472 65 Size: 1183 3050 174 1396 Fragments arranged by size: 3050 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4447 4634 1700 Size: 110 2637 187 2869 Fragments arranged by size: 2869 2637 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5803 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5803 BssSI C'ACGA_G Cuts at: 3863 4875 3863 Size: 1012 4791 BstAPI GCAn_nnn'nTGC Cuts at: 794 2887 794 Size: 2093 3710 BstBI TT'CG_AA Cuts at: 4607 4607 Size: 5803 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5803 BstYI r'GATC_y Cuts at: 1972 2230 3133 3500 3885 3902 5354 5440 5452 Size: 258 903 367 385 17 1452 86 12 Cuts at: 5452 5798 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 903 385 367 346 258 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2531 2531 Size: 5803 BtgI C'CryG_G Cuts at: 513 1065 2581 513 Size: 552 1516 3735 Fragments arranged by size: 3735 1516 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5064 Fragments arranged by size: 5064 683 56 BtsI GCAGTG_nn' Cuts at: 3319 4113 4133 3319 Size: 794 20 4989 Fragments arranged by size: 4989 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5803 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5768 DraI TTT'AAA Cuts at: 3107 3107 Size: 5803 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4810 809 Size: 507 3494 1802 Fragments arranged by size: 3494 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2865 3250 4139 Size: 54 1425 50 275 18 1035 385 889 Cuts at: 4139 5768 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1035 889 385 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3250 1537 Size: 275 1438 4090 Fragments arranged by size: 4090 1438 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2291 3733 1353 Size: 938 1442 3423 Fragments arranged by size: 3423 1442 938 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2717 4390 4764 4910 387 Size: 1064 138 1128 1673 374 146 1280 Fragments arranged by size: 1673 1280 1128 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2067 2744 2969 3866 4463 5250 5518 Size: 393 435 677 225 897 597 787 268 Cuts at: 5518 1239 Size: 1524 Fragments arranged by size: 1524 897 787 677 597 435 393 268 225 EcoNI CCTnn'n_nnAGG Cuts at: 2165 2696 2797 2165 Size: 531 101 5171 Fragments arranged by size: 5171 531 101 EcoO109I rG'GnC_Cy Cuts at: 2325 2337 2365 2511 2325 Size: 12 28 146 5617 Fragments arranged by size: 5617 146 28 12 EcoRI G'AATT_C Cuts at: 3112 3490 3112 Size: 378 5425 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2273 2273 Size: 5803 FspI TGC'GCA Cuts at: 2891 4309 2891 Size: 1418 4385 HaeII r_GCGC'y Cuts at: 1678 4950 1678 Size: 3272 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 2222 2254 2740 Size: 32 135 32 491 32 127 32 486 Cuts at: 2740 2772 4444 4476 4518 4550 1373 Size: 32 1672 32 42 32 2626 Fragments arranged by size: 2626 1672 491 486 135 127 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2449 3147 3393 669 Size: 988 281 511 698 246 3079 Fragments arranged by size: 3079 988 698 511 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5803 HpaI GTT'AAC Cuts at: 3393 3393 Size: 5803 KpnI G_GTAC'C Cuts at: 3128 3128 Size: 5803 MfeI C'AATT_G Cuts at: 3402 3402 Size: 5803 MluI A'CGCG_T Cuts at: 5679 5679 Size: 5803 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2046 2358 2478 4917 5101 1305 Size: 741 312 120 2439 184 2007 Fragments arranged by size: 2439 2007 741 312 184 120 MscI TGG'CCA Cuts at: 10 64 1489 1832 2867 10 Size: 54 1425 343 1035 2946 Fragments arranged by size: 2946 1425 1035 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 2876 3761 4120 4279 518 Size: 648 1572 138 885 359 159 2042 Fragments arranged by size: 2042 1572 885 648 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2371 3219 3896 5044 5289 1606 Size: 765 848 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 765 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5251 NdeI CA'TA_TG Cuts at: 387 387 Size: 5803 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5803 NotI GC'GGCC_GC Cuts at: 3250 3250 Size: 5803 NspI r_CATG'y Cuts at: 1132 1229 2674 3161 4706 1132 Size: 97 1445 487 1545 2229 Fragments arranged by size: 2229 1545 1445 487 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5803 PciI A'CATG_T Cuts at: 4702 4702 Size: 5803 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4901 PfoI T'CCnGG_A Cuts at: 2009 2083 2265 2009 Size: 74 182 5547 Fragments arranged by size: 5547 182 74 PmeI GTTT'AAAC Cuts at: 3107 3107 Size: 5803 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3872 3904 5385 5417 3872 Size: 32 1481 32 4258 Fragments arranged by size: 4258 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2337 2511 2337 Size: 174 5629 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5803 PsiI TTA'TAA Cuts at: 3373 3373 Size: 5803 PspOMI G'GGCC_C Cuts at: 2364 2364 Size: 5803 PstI C_TGCA'G Cuts at: 830 3090 3155 830 Size: 2260 65 3478 Fragments arranged by size: 3478 2260 65 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5803 SalI G'TCGA_C Cuts at: 1936 3145 1936 Size: 1209 4594 SbfI CC_TGCA'GG Cuts at: 3155 3155 Size: 5803 ScaI AGT'ACT Cuts at: 4051 5763 4051 Size: 1712 4091 SfcI C'TryA_G Cuts at: 826 1045 3086 3151 4286 4967 5158 826 Size: 219 2041 65 1135 681 191 1471 Fragments arranged by size: 2041 1471 1135 681 219 191 65 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5803 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5803 SmaI CCC'GGG Cuts at: 1872 3130 1872 Size: 1258 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3907 4808 5070 820 Size: 197 926 1964 901 262 1553 Fragments arranged by size: 1964 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3594 493 Size: 3101 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5803 SphI G_CATG'C Cuts at: 1132 3161 1132 Size: 2029 3774 SspI AAT'ATT Cuts at: 5 52 3727 5 Size: 47 3675 2081 Fragments arranged by size: 3675 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5803 StyI C'CwwG_G Cuts at: 513 1065 3075 3242 513 Size: 552 2010 167 3074 Fragments arranged by size: 3074 2010 552 167 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2797 3830 3847 4000 4185 4630 325 Size: 1255 1217 1033 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1217 1033 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4049 5761 96 Size: 274 80 33 51 1443 2072 1712 138 Fragments arranged by size: 2072 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2247 2279 5763 5795 2247 Size: 32 3484 32 2255 Fragments arranged by size: 3484 2255 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5803 XbaI T'CTAG_A Cuts at: 3139 3139 Size: 5803 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2273 2579 2273 Size: 306 5497 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5803 XmaI C'CCGG_G Cuts at: 1870 3128 1870 Size: 1258 4545 XmnI GAAnn'nnTTC Cuts at: 3932 3932 Size: 5803 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3611 276 Size: 53 83 186 936 2077 2468 Fragments arranged by size: 2468 2077 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI