(Linear) (Six-base) MAPSORT of: KIEE0618.seq  Check: 1116  from: 1  to: 1098



KIEE0618.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     749    1098 
   Size:        749     349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     267     675     882    1098 
   Size:        267     408     207     216

  Fragments arranged by size: 

                 408     267     216     207
 
ApaI G_GGCC'C

Cuts at:      0     363    1098 
   Size:        363     735
 
ApoI r'AATT_y

Cuts at:      0     146    1098 
   Size:        146     952
 
AvaI C'yCGr_G

Cuts at:      0     356    1098 
   Size:        356     742
 
BanII G_rGCy'C

Cuts at:      0     235     363     727    1098 
   Size:        235     128     364     371

  Fragments arranged by size: 

                 371     364     235     128
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     622    1098 
   Size:        622     476
 
BglII A'GATC_T

Cuts at:      0     225    1098 
   Size:        225     873
 
Bme1580I G_kGCm'C

Cuts at:      0     363    1098 
   Size:        363     735
 
BmgBI CAC'GTC

Cuts at:      0     642    1098 
   Size:        642     456
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     739     964    1098 
   Size:         62     677     225     134

  Fragments arranged by size: 

                 677     225     134      62
 
Bpu10I CC'TnA_GC

Cuts at:      0     547     692    1098 
   Size:        547     145     406

  Fragments arranged by size: 

                 547     406     145
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     536    1098 
   Size:        536     562
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      79     715    1098 
   Size:         79     636     383

  Fragments arranged by size: 

                 636     383      79
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640     895    1098 
   Size:        640     255     203

  Fragments arranged by size: 

                 640     255     203
 
BsmI GAATG_Cn'

Cuts at:      0     538    1098 
   Size:        538     560
 
Bsp1286I G_dGCh'C

Cuts at:      0     235     363     727    1098 
   Size:        235     128     364     371

  Fragments arranged by size: 

                 371     364     235     128
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     882    1098 
   Size:        882     216
 
BstYI r'GATC_y

Cuts at:      0     225    1098 
   Size:        225     873
 
Bsu36I CC'TnA_GG

Cuts at:      0     526    1098 
   Size:        526     572
 
BtgI C'CryG_G

Cuts at:      0     576    1098 
   Size:        576     522
 
EaeI y'GGCC_r

Cuts at:      0     860    1098 
   Size:        860     238
 
EarI CTCTTCn'nnn_

Cuts at:      0     286    1098 
   Size:        286     812
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     712    1098 
   Size:        712     386
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     739     964    1098 
   Size:         62     677     225     134

  Fragments arranged by size: 

                 677     225     134      62
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     160     691     792    1098 
   Size:        160     531     101     306

  Fragments arranged by size: 

                 531     306     160     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0     320     332     360     506    1098 
   Size:        320      12      28     146     592

  Fragments arranged by size: 

                 592     320     146      28      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     268    1098 
   Size:        268     830
 
FspI TGC'GCA

Cuts at:      0     886    1098 
   Size:        886     212
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      58      90     217     249     735     767    1098 
   Size:         58      32     127      32     486      32     331

  Fragments arranged by size: 

                 486     331     127      58      32      32      32
 
HincII GTy'rAC

Cuts at:      0     444    1098 
   Size:        444     654
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     353     473    1098 
   Size:         41     312     120     625

  Fragments arranged by size: 

                 625     312     120      41
 
MscI TGG'CCA

Cuts at:      0     862    1098 
   Size:        862     236
 
MslI CAynn'nnrTG

Cuts at:      0     733     871    1098 
   Size:        733     138     227

  Fragments arranged by size: 

                 733     227     138
 
MspA1I CmG'CkG

Cuts at:      0     366    1098 
   Size:        366     732
 
NspI r_CATG'y

Cuts at:      0     669    1098 
   Size:        669     429
 
PfoI T'CCnGG_A

Cuts at:      0       4      78     260    1098 
   Size:          4      74     182     838

  Fragments arranged by size: 

                 838     182      74       4
 
PpuMI rG'GwC_Cy

Cuts at:      0     332     506    1098 
   Size:        332     174     592

  Fragments arranged by size: 

                 592     332     174
 
PspOMI G'GGCC_C

Cuts at:      0     359    1098 
   Size:        359     739
 
PstI C_TGCA'G

Cuts at:      0    1085    1098 
   Size:       1085      13
 
SfcI C'TryA_G

Cuts at:      0    1081    1098 
   Size:       1081      17
 
StyI C'CwwG_G

Cuts at:      0    1070    1098 
   Size:       1070      28
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     792    1098 
   Size:        792     306
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     242     274    1098 
   Size:        242      32     824

  Fragments arranged by size: 

                 824     242      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     268     574    1098 
   Size:        268     306     524

  Fragments arranged by size: 

                 524     306     268
 

 Enzymes that do cut:

     AhdI    AlwNI     ApaI     ApoI     AvaI    BanII     BbsI    BglII
 Bme1580I    BmgBI     BpmI   Bpu10I     BsaI    BseRI     BsgI     BsmI
 Bsp1286I   BstAPI    BstYI   Bsu36I     BtgI     EaeI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI     FspI    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NspI     PfoI    PpuMI   PspOMI     PstI
     SfcI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI     BmrI     BmtI     BplI
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoRI    EcoRV     FseI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI