Restriction map for
pFN21AE0608
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CpoI DraIII DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 460 TCCGGA T^CCGGA
AflIII 787 ACGTGT A^C[GA][CT]GT
AvaI 158 CTCGGG C^[CT]CG[GA]G
BamHI 931 GGATCC G^GATCC
BanI 814 GGTGCC G^G[CT][GA]CC
BanII 750 GAGCTC G[GA]GC[CT]^C
BclI 449 TGATCA T^GATCA
BcnI 986 CCCGG CC^[GC]GG
BfaI 133 CTAG C^TAG
BglII 577 AGATCT A^GATCT
BlpI 747 GCTGAGC GC^T[ACGT]AGC
BpmI 804 CTCCAG CTGGAG(16/14)
Bpu1102I 747 GCTGAGC GC^T[ACGT]AGC
BsaI 215 GGTCTC GGTCTC(1/5)
BseAI 460 TCCGGA T^CCGGA
BsmI 300 GAATGC GAATGC(1/-1)
BsoBI 158 CTCGGG C^[CT]CG[GA]G
BspDI 713 ATCGAT AT^CGAT
BspEI 460 TCCGGA T^CCGGA
BsrDI 32 GCAATG GCAATG(2/0)
BsrFI 91 ACCGGC [GA]^CCGG[CT]
BstXI 755 CCATGACGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 747 GCTGAGC GC^T[ACGT]AGC
CfoI 56 GCGC GCG^C
Cfr10I 91 ACCGGC [GA]^CCGG[CT]
ClaI 713 ATCGAT AT^CGAT
DraI 540 TTTAAA TTT^AAA
DrdI 786 GACGTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 682 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 860 CCAAGG C^C[AT][AT]GG
FbaI 449 TGATCA T^GATCA
HhaI 56 GCGC GCG^C
HinP1I 56 GCGC G^CGC
HincII 442 GTTGAC GT[CT]^[GA]AC
HindII 442 GTTGAC GT[CT]^[GA]AC
HindIII 226 AAGCTT A^AGCTT
MaeI 133 CTAG C^TAG
MaeII 787 ACGT A^CGT
MroI 460 TCCGGA T^CCGGA
NciI 986 CCCGG CC^[GC]GG
NheI 132 GCTAGC G^CTAGC
NspI 968 GCATGT [GA]CATG^[CT]
PpuMI 976 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 732 CAGCTG CAG^CTG
SacI 750 GAGCTC GAGCT^C
SapI 990 GAAGAGC GCTCTTC(1/4)
StyI 860 CCAAGG C^C[AT][AT]GG
TaqI 714 TCGA T^CGA
Tsp45I 203 GTGAC ^GT[GC]AC
TthHB8I 714 TCGA T^CGA
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 46 GCGG CCGC(-3/-1)
929 GCGG
AcsI 272 AAATTC [GA]^AATT[CT]
1027 AAATTT
AfaI 573 GTAC GT^AC
836 GTAC
AlwI 932 GATCC GGATC(4/5)
931 GGATC
ApoI 272 AAATTC [GA]^AATT[CT]
1027 AAATTT
AspHI 411 GAGCAC G[AT]GC[AT]^C
750 GAGCTC
BmyI 411 GAGCAC G[AGT]GC[ACT]^C
750 GAGCTC
BseRI 309 CTCCTC GAGGAG(10/8)
882 GAGGAG
BsiHKAI 411 GAGCAC G[AT]GC[AT]^C
750 GAGCTC
BsmFI 162 GGGAC GGGAC(10/14)
976 GGGAC
Bsp1286I 411 GAGCAC G[AGT]GC[ACT]^C
750 GAGCTC
BspMI 467 ACCTGC ACCTGC(4/8)
534 GCAGGT
BsrI 632 CCAGT ACTGG(1/-1)
722 CCAGT
BstF5I 654 GGATG GGATG(2/0)
870 GGATG
BstYI 577 AGATCT [GA]^GATC[CT]
931 GGATCC
DraII 286 AGGCCCC [GA]G^G[ACGT]CC[CT]
976 GGGACCC
EarI 990 GAAGAG CTCTTC(1/4)
1057 GAAGAG
EcoO109I 286 AGGCCCC [GA]G^G[ACGT]CC[CT]
976 GGGACCC
FokI 654 GGATG GGATG(9/13)
870 GGATG
Ksp632I 990 GAAGAG CTCTTC(1/4)
1057 GAAGAG
MflI 577 AGATCT [GA]^GATC[CT]
931 GGATCC
MslI 610 CATGGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
756 CATGACGATG
MspA1I 732 CAGCTG C[AC]G^C[GT]G
927 CAGCGG
PleI 483 GACTC GAGTC(4/5)
802 GACTC
PstI 532 CTGCAG CTGCA^G
583 CTGCAG
RsaI 573 GTAC GT^AC
836 GTAC
SfaNI 616 GATGC GCATC(5/9)
911 GCATC
SfcI 532 CTGCAG C^T[GA][CT]AG
583 CTGCAG
XhoII 577 AGATCT [GA]^GATC[CT]
931 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AluI AvaII BbvI BsaJI BsaWI
BsiYI BslI BsmAI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI TfiI Tru9I TseI Tsp509I
TspRI