(Circular) (Six-base) MAPSORT of: pFN21AE0608.seq Check: 5336 from: 1 to: 5782 pFN21AE0608 5782 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2809 3845 5229 5497 1632 Size: 1177 1036 1384 268 1917 Fragments arranged by size: 1917 1384 1177 1036 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 2792 4681 5658 1203 Size: 1589 1889 977 1327 Fragments arranged by size: 1889 1589 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2647 1184 Size: 1463 4319 AlwNI CAG_nnn'CTG Cuts at: 3525 5097 3525 Size: 1572 4210 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2277 3032 3091 3317 3469 5578 1493 Size: 784 755 59 226 152 2109 1697 Fragments arranged by size: 2109 1697 784 755 226 152 59 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2163 3107 1870 Size: 73 220 944 4545 Fragments arranged by size: 4545 944 220 73 BamHI G'GATC_C Cuts at: 2936 3112 3479 2936 Size: 176 367 5239 Fragments arranged by size: 5239 367 176 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 3103 4558 618 Size: 325 217 627 1032 284 1455 1842 Fragments arranged by size: 1842 1455 1032 627 325 284 217 BanII G_rGCy'C Cuts at: 1391 2759 3101 1391 Size: 1368 342 4072 Fragments arranged by size: 4072 1368 342 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 2454 1548 Size: 906 4876 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2582 5777 2582 Size: 3195 2587 BlpI GC'TnA_GC Cuts at: 2753 3199 2753 Size: 446 5336 Bme1580I G_kGCm'C Cuts at: 1790 3843 4999 1790 Size: 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 BmgBI CAC'GTC Cuts at: 1318 2793 1318 Size: 1475 4307 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2631 3513 4471 Size: 416 53 83 399 24 1214 882 958 Cuts at: 4471 442 Size: 1753 Fragments arranged by size: 1753 1214 958 882 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2141 1056 Size: 1085 4697 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2794 4442 1239 Size: 1555 1648 2579 Fragments arranged by size: 2579 1648 1555 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2226 4445 882 Size: 617 727 2219 2219 Fragments arranged by size: 2219 2219 727 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2465 2712 2910 4215 4613 4887 5034 5445 2465 Size: 247 198 1305 398 274 147 411 2802 Fragments arranged by size: 2802 1305 411 398 274 247 198 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2004 2034 2905 2935 1178 Size: 30 165 30 601 30 871 30 4025 Fragments arranged by size: 4025 871 601 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2305 2902 1186 Size: 1119 597 4066 Fragments arranged by size: 4066 1119 597 BseYI C'CCAG_C Cuts at: 2063 2359 2698 4985 5481 2063 Size: 296 339 2287 496 2364 Fragments arranged by size: 2364 2287 496 339 296 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5209 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2420 2759 3843 3928 4999 2420 Size: 339 1084 85 1071 3203 Fragments arranged by size: 3203 1084 1071 339 85 BsmI GAATG_Cn' Cuts at: 2311 3293 3386 2311 Size: 982 93 4707 Fragments arranged by size: 4707 982 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2420 2759 3101 3843 3928 4999 1391 Size: 399 630 339 342 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 630 399 342 339 85 BspEI T'CCGG_A Cuts at: 2465 5445 2465 Size: 2980 2802 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2481 2530 3137 844 Size: 1637 49 607 3489 Fragments arranged by size: 3489 1637 607 49 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2044 4277 4451 65 Size: 1183 796 2233 174 1396 Fragments arranged by size: 2233 1396 1183 796 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 4426 4613 1700 Size: 110 286 2330 187 2869 Fragments arranged by size: 2869 2330 286 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5782 BstXI CCAn_nnnn'nTGG Cuts at: 2767 2767 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2582 2936 3112 3479 3864 3881 5333 5419 Size: 610 354 176 367 385 17 1452 86 Cuts at: 5419 5431 5777 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 610 385 367 354 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5230 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 3298 4092 4112 3298 Size: 794 20 4968 Fragments arranged by size: 4968 794 20 ClaI AT'CG_AT Cuts at: 1554 2719 1554 Size: 1165 4617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 2547 3086 2547 Size: 539 5243 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2797 4789 809 Size: 507 1481 1992 1802 Fragments arranged by size: 1992 1802 1481 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3229 4118 5747 Size: 54 1425 50 275 18 1399 889 1629 Cuts at: 5747 8 Size: 43 Fragments arranged by size: 1629 1425 1399 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2990 3057 3712 1353 Size: 1637 67 655 3423 Fragments arranged by size: 3423 1637 655 67 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2066 4369 4743 4889 387 Size: 1064 138 477 2303 374 146 1280 Fragments arranged by size: 2303 1280 1064 477 374 146 138 EcoICRI GAG'CTC Cuts at: 2757 2757 Size: 5782 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2794 2809 3845 4442 5229 5497 1239 Size: 393 1162 15 1036 597 787 268 1524 Fragments arranged by size: 1524 1162 1036 787 597 393 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2691 2691 Size: 5782 EcoO109I rG'GnC_Cy Cuts at: 2292 2982 2292 Size: 690 5092 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3043 3043 Size: 5782 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 4929 1678 Size: 3251 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2473 2505 2567 2599 2651 Size: 32 135 32 901 32 62 32 52 Cuts at: 2651 2683 4423 4455 4497 4529 1373 Size: 32 1740 32 42 32 2626 Fragments arranged by size: 2626 1740 901 135 62 52 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2449 3126 3372 669 Size: 988 281 511 677 246 3079 Fragments arranged by size: 3079 988 677 511 281 246 HindIII A'AGCT_T Cuts at: 748 2231 748 Size: 1483 4299 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2619 2765 3740 4099 4258 518 Size: 648 1453 146 975 359 159 2042 Fragments arranged by size: 2042 1453 975 648 359 159 146 MspA1I CmG'CkG Cuts at: 1606 2739 2934 3198 3875 5023 5268 1606 Size: 1133 195 264 677 1148 245 2120 Fragments arranged by size: 2120 1148 1133 677 264 245 195 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 2137 1052 Size: 1085 4697 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2977 3140 4685 1132 Size: 97 1748 163 1545 2229 Fragments arranged by size: 2229 1748 1545 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2982 2982 Size: 5782 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2541 2592 3134 830 Size: 1711 51 542 3478 Fragments arranged by size: 3478 1711 542 51 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2739 2739 Size: 5782 SacI G_AGCT'C Cuts at: 2759 2759 Size: 5782 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SanDI GG'GwC_CC Cuts at: 2982 2982 Size: 5782 SapI GCTCTTCn'nnn_ Cuts at: 2990 2990 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2537 2588 3130 4265 4946 5137 826 Size: 219 1492 51 542 1135 681 191 1471 Fragments arranged by size: 1492 1471 1135 681 542 219 191 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3886 4787 5049 820 Size: 197 926 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5782 StyI C'CwwG_G Cuts at: 513 1065 2865 3221 513 Size: 552 1800 356 3074 Fragments arranged by size: 3074 1800 552 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2560 3809 3826 3979 4164 4609 325 Size: 1255 980 1249 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1249 980 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 2840 4028 Size: 274 80 33 51 1443 600 263 1188 Cuts at: 4028 5740 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1188 600 274 263 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI