(Circular) (Six-base) MAPSORT of: pFN21AE0596.seq Check: 7975 from: 1 to: 5173 pFN21AE0596 5173 bp With 182 enzymes: SgfI * October 20, 2009 10:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2983 278 Size: 53 83 186 936 1447 2468 Fragments arranged by size: 2468 1447 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2516 1937 Size: 579 4594 Acc65I G'GTAC_C Cuts at: 2494 2494 Size: 5173 AclI AA'CG_TT Cuts at: 1563 3300 3673 1563 Size: 1737 373 3063 Fragments arranged by size: 3063 1737 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3236 4620 4888 1632 Size: 1604 1384 268 1917 Fragments arranged by size: 1917 1604 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4976 AflIII A'CryG_T Cuts at: 1203 4072 5049 1203 Size: 2869 977 1327 Fragments arranged by size: 2869 1327 977 AgeI A'CCGG_T Cuts at: 4004 4004 Size: 5173 AhdI GACnn_n'nnGTC Cuts at: 3902 3902 Size: 5173 AleI CACnn'nnGTG Cuts at: 1166 2396 1166 Size: 1230 3943 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5173 AlwNI CAG_nnn'CTG Cuts at: 2434 2916 4488 2434 Size: 482 1572 3119 Fragments arranged by size: 3119 1572 482 ApaI G_GGCC'C Cuts at: 2075 2370 2075 Size: 295 4878 ApaLI G'TGCA_C Cuts at: 3230 4386 3230 Size: 1156 4017 ApoI r'AATT_y Cuts at: 1493 2482 2708 2860 4969 1493 Size: 989 226 152 2109 1697 Fragments arranged by size: 2109 1697 989 226 152 AseI AT'TA_AT Cuts at: 160 1032 3727 160 Size: 872 2695 1606 Fragments arranged by size: 2695 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5173 AvaI C'yCGr_G Cuts at: 1870 1943 2498 1870 Size: 73 555 4545 Fragments arranged by size: 4545 555 73 BamHI G'GATC_C Cuts at: 2503 2870 2503 Size: 367 4806 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2303 2494 3949 618 Size: 325 217 627 366 150 191 1455 1842 Fragments arranged by size: 1842 1455 627 366 325 217 191 150 BanII G_rGCy'C Cuts at: 1391 2033 2075 2188 2359 2370 2492 1391 Size: 642 42 113 171 11 122 4072 Fragments arranged by size: 4072 642 171 122 113 42 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3364 3398 1903 Size: 34 1427 34 3678 Fragments arranged by size: 3678 1427 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3065 4275 3065 Size: 1210 3963 BclI T'GATC_A Cuts at: 1548 1548 Size: 5173 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3784 136 Size: 107 122 71 631 605 2112 1525 Fragments arranged by size: 2112 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5168 5168 Size: 5173 BlpI GC'TnA_GC Cuts at: 2590 2590 Size: 5173 Bme1580I G_kGCm'C Cuts at: 1790 2075 2156 2308 2370 3234 4390 1790 Size: 285 81 152 62 864 1156 2573 Fragments arranged by size: 2573 1156 864 285 152 81 62 BmgBI CAC'GTC Cuts at: 1318 2398 1318 Size: 1080 4093 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2904 3862 442 Size: 416 53 83 399 24 1487 958 1753 Fragments arranged by size: 1753 1487 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5173 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2157 3833 1239 Size: 918 1676 2579 Fragments arranged by size: 2579 1676 918 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3298 4163 4461 3298 Size: 865 298 4010 Fragments arranged by size: 4010 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3836 882 Size: 617 2337 2219 Fragments arranged by size: 2337 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2964 493 Size: 711 1760 2702 Fragments arranged by size: 2702 1760 711 BsaBI GATnn'nnATC Cuts at: 5167 5167 Size: 5173 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2980 3362 3997 275 Size: 53 83 186 936 1447 382 635 1451 Fragments arranged by size: 1451 1447 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3606 4004 4278 4425 4836 3606 Size: 398 274 147 411 3943 Fragments arranged by size: 3943 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4948 Fragments arranged by size: 4948 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 1186 Size: 1087 4086 BseYI C'CCAG_C Cuts at: 2026 2056 2067 2149 2313 2439 4376 4872 2026 Size: 30 11 82 164 126 1937 496 2327 Fragments arranged by size: 2327 1937 496 164 126 82 30 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4600 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2623 3384 4412 1540 Size: 275 186 622 761 1028 2301 Fragments arranged by size: 2301 1028 761 622 275 186 BsiHKAI G_wGCw'C Cuts at: 3234 3319 4390 3234 Size: 85 1071 4017 Fragments arranged by size: 4017 1071 85 BsmI GAATG_Cn' Cuts at: 2684 2777 2684 Size: 93 5080 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2075 2156 2188 2308 2359 2370 Size: 399 243 42 81 32 120 51 11 Cuts at: 2370 2492 3234 3319 4390 1391 Size: 122 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 243 122 120 85 81 51 42 32 11 BspEI T'CCGG_A Cuts at: 4836 4836 Size: 5173 BspHI T'CATG_A Cuts at: 3062 4792 3062 Size: 1730 3443 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2528 844 Size: 1684 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3060 1394 Size: 429 1237 3507 Fragments arranged by size: 3507 1237 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3668 3842 65 Size: 1183 2420 174 1396 Fragments arranged by size: 2420 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2285 2376 3817 4004 1700 Size: 110 475 91 1441 187 2869 Fragments arranged by size: 2869 1441 475 187 110 91 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5173 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5173 BssSI C'ACGA_G Cuts at: 3233 4245 3233 Size: 1012 4161 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 794 Size: 1640 3533 BstBI TT'CG_AA Cuts at: 3977 3977 Size: 5173 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5173 BstYI r'GATC_y Cuts at: 1972 2503 2870 3255 3272 4724 4810 4822 5168 Size: 531 367 385 17 1452 86 12 346 Cuts at: 5168 1972 Size: 1977 Fragments arranged by size: 1977 1452 531 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2083 2094 2226 2299 2359 513 Size: 552 939 79 11 132 73 60 3327 Fragments arranged by size: 3327 939 552 132 79 73 60 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4434 Fragments arranged by size: 4434 683 56 BtsI GCAGTG_nn' Cuts at: 2689 3483 3503 2689 Size: 794 20 4359 Fragments arranged by size: 4359 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5173 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5138 DraI TTT'AAA Cuts at: 2477 2477 Size: 5173 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4180 809 Size: 507 2864 1802 Fragments arranged by size: 2864 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2097 2620 Size: 54 1425 50 275 18 177 90 523 Cuts at: 2620 3509 5138 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 523 275 177 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2620 1537 Size: 275 808 4090 Fragments arranged by size: 4090 808 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3103 1353 Size: 1750 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3760 4134 4280 387 Size: 1064 138 2171 374 146 1280 Fragments arranged by size: 2171 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2157 3236 3833 4620 4888 1239 Size: 393 525 1079 597 787 268 1524 Fragments arranged by size: 1524 1079 787 597 525 393 268 EcoO109I rG'GnC_Cy Cuts at: 2071 2072 2142 2367 2071 Size: 1 70 225 4877 Fragments arranged by size: 4877 225 70 1 EcoRI G'AATT_C Cuts at: 2482 2860 2482 Size: 378 4795 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2287 Size: 5173 FspI TGC'GCA Cuts at: 3679 3679 Size: 5173 HaeII r_GCGC'y Cuts at: 1678 2330 4320 1678 Size: 652 1990 2531 Fragments arranged by size: 2531 1990 652 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 3814 3846 3888 Size: 32 135 32 527 32 1683 32 42 Cuts at: 3888 3920 1373 Size: 32 2626 Fragments arranged by size: 2626 1683 527 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2517 2763 669 Size: 988 281 579 246 3079 Fragments arranged by size: 3079 988 579 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5173 HpaI GTT'AAC Cuts at: 2763 2763 Size: 5173 KpnI G_GTAC'C Cuts at: 2498 2498 Size: 5173 MfeI C'AATT_G Cuts at: 2772 2772 Size: 5173 MluI A'CGCG_T Cuts at: 5049 5049 Size: 5173 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4287 4471 1305 Size: 2982 184 2007 Fragments arranged by size: 2982 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3351 Fragments arranged by size: 3351 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 3131 3490 3649 518 Size: 648 1230 735 359 159 2042 Fragments arranged by size: 2042 1230 735 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2206 2292 2424 2431 2589 3266 4414 Size: 450 150 86 132 7 158 677 1148 Cuts at: 4414 4659 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 450 245 158 150 132 86 7 NaeI GCC'GGC Cuts at: 2287 2378 2287 Size: 91 5082 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3682 Fragments arranged by size: 3682 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5173 NgoMIV G'CCGG_C Cuts at: 2285 2376 2285 Size: 91 5082 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5173 NotI GC'GGCC_GC Cuts at: 2620 2620 Size: 5173 NspI r_CATG'y Cuts at: 1132 1229 2531 4076 1132 Size: 97 1302 1545 2229 Fragments arranged by size: 2229 1545 1302 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5173 PciI A'CATG_T Cuts at: 4072 4072 Size: 5173 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5173 PmeI GTTT'AAAC Cuts at: 2477 2477 Size: 5173 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3242 3274 4755 4787 3242 Size: 32 1481 32 3628 Fragments arranged by size: 3628 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2142 2142 Size: 5173 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5173 PsiI TTA'TAA Cuts at: 2743 2743 Size: 5173 PspOMI G'GGCC_C Cuts at: 2071 2366 2071 Size: 295 4878 PstI C_TGCA'G Cuts at: 830 2055 2343 2525 830 Size: 1225 288 182 3478 Fragments arranged by size: 3478 1225 288 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5173 PvuII CAG'CTG Cuts at: 2056 2206 2292 2056 Size: 150 86 4937 Fragments arranged by size: 4937 150 86 SalI G'TCGA_C Cuts at: 1936 2515 1936 Size: 579 4594 SbfI CC_TGCA'GG Cuts at: 2525 2525 Size: 5173 ScaI AGT'ACT Cuts at: 3421 5133 3421 Size: 1712 3461 SfcI C'TryA_G Cuts at: 826 1045 2051 2339 2521 3656 4337 4528 826 Size: 219 1006 288 182 1135 681 191 1471 Fragments arranged by size: 1471 1135 1006 681 288 219 191 182 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5173 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5173 SmaI CCC'GGG Cuts at: 1872 2500 1872 Size: 628 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3277 4178 4440 820 Size: 197 926 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2964 493 Size: 2471 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5173 SphI G_CATG'C Cuts at: 1132 2531 1132 Size: 1399 3774 SspI AAT'ATT Cuts at: 5 52 3097 5 Size: 47 3045 2081 Fragments arranged by size: 3045 2081 47 StuI AGG'CCT Cuts at: 1517 2241 1517 Size: 724 4449 StyI C'CwwG_G Cuts at: 513 1065 2004 2612 513 Size: 552 939 608 3074 Fragments arranged by size: 3074 939 608 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2171 2327 3200 3217 3370 3555 4000 Size: 1255 591 156 873 17 153 185 445 Cuts at: 4000 325 Size: 1498 Fragments arranged by size: 1498 1255 873 591 445 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3419 5131 96 Size: 274 80 33 51 1443 1442 1712 138 Fragments arranged by size: 1712 1443 1442 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5133 5165 5133 Size: 32 5141 Tth111I GACn'n_nGTC Cuts at: 1316 2221 1316 Size: 905 4268 XbaI T'CTAG_A Cuts at: 2509 2509 Size: 5173 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2205 2205 Size: 5173 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5173 XmaI C'CCGG_G Cuts at: 1870 2498 1870 Size: 628 4545 XmnI GAAnn'nnTTC Cuts at: 3302 3302 Size: 5173 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2981 276 Size: 53 83 186 936 1447 2468 Fragments arranged by size: 2468 1447 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI