Restriction map for
pFN21AE0595
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspHI AspI AviII AvrII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DdeI DraI DraII DraIII DrdI
DsaI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MaeI MluI MseI
MslI MspA1I MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfcI SfiI SfuI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
TspRI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccIII 174 TCCGGA T^CCGGA
AcyI 465 GGCGCC G[GA]^CG[CT]C
AfaI 126 GTAC GT^AC
AhdI 78 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 78 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 380 TGGCCA TGG^CCA
BamHI 307 GGATCC G^GATCC
BanII 178 GAGCCC G[GA]GC[CT]^C
BbeI 465 GGCGCC GGCGC^C
BglII 416 AGATCT A^GATCT
BmyI 178 GAGCCC G[AGT]GC[ACT]^C
BsaBI 411 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 465 GGCGCC G[GA]^CG[CT]C
BseAI 174 TCCGGA T^CCGGA
BseRI 325 CTCCTC GAGGAG(10/8)
Bsp1286I 178 GAGCCC G[AGT]GC[ACT]^C
BspEI 174 TCCGGA T^CCGGA
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrFI 233 ACCGGC [GA]^CCGG[CT]
BsrI 219 CCAGT ACTGG(1/-1)
BstAPI 482 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 483 CATCC GGATG(2/0)
CfoI 466 GCGC GCG^C
Cfr10I 233 ACCGGC [GA]^CCGG[CT]
EaeI 380 TGGCCA [CT]^GGCC[GA]
Eam1105I 78 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 30 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 270 CCCGC CCCGC(4/6)
FokI 483 CATCC GGATG(9/13)
HaeII 465 GGCGCC [GA]GCGC^[CT]
HhaI 466 GCGC GCG^C
Hin1I 465 GGCGCC G[GA]^CG[CT]C
HinP1I 466 GCGC G^CGC
HphI 302 TCACC GGTGA(8/7)
KasI 465 GGCGCC G^GCGCC
MaeII 264 ACGT A^CGT
MaeIII 266 GTGAC ^GT[ACGT]AC
MamI 411 GATTCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 503 CAATTG C^AATTG
MluNI 380 TGGCCA TGG^CCA
MroI 174 TCCGGA T^CCGGA
MscI 380 TGGCCA TGG^CCA
MunI 503 CAATTG C^AATTG
NarI 465 GGCGCC GG^CGCC
NlaIII 215 CATG CATG^
RsaI 126 GTAC GT^AC
SfoI 465 GGCGCC GGC^GCC
SgrAI 232 CACCGGCG C[GA]^CCGG[CT]G
Tsp45I 266 GTGAC ^GT[GC]AC
Tsp509I 504 AATT ^AATT
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# TWO CUT ENZYMES ( 14 enzymes) #
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AvaII 49 GGACC G^G[AT]CC
186 GGTCC
BanI 443 GGCACC G^G[CT][GA]CC
465 GGCGCC
BsmAI 261 GAGAC GTCTC(1/5)
388 GAGAC
BsmBI 261 GAGACG CGTCTC(1/5)
388 GAGACG
BsmFI 48 GGGAC GGGAC(10/14)
450 GGGAC
BstYI 307 GGATCC [GA]^GATC[CT]
416 AGATCT
MflI 307 GGATCC [GA]^GATC[CT]
416 AGATCT
PleI 84 GAGTC GAGTC(4/5)
403 GAGTC
SmaI 129 CCCGGG CCC^GGG
256 CCCGGG
TfiI 411 GATTC G^A[AT]TC
429 GAATC
XcmI 143 CCAAAGAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCAGGGAAGAGCTGG
XhoII 307 GGATCC [GA]^GATC[CT]
416 AGATCT
XmaCI 129 CCCGGG C^CCGGG
256 CCCGGG
XmaI 129 CCCGGG C^CCGGG
256 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI AvaI BbvI
BcnI BsaJI BsaWI BsiYI BslI
BsoBI BsrDI BssKI BstNI Cac8I
Cfr13I DpnII EarI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIV
SapI Sau3AI Sau96I ScrFI SfaNI
TaqI TseI TthHB8I