Restriction map for
pFN21AE0594
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspHI
AspI AviII AvrII BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsgI BsiEI BsiHKAI BsiWI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MaeII MaeIII MamI MluI MseI
MslI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccII 265 CGCG CG^CG
AccIII 174 TCCGGA T^CCGGA
AcyI 465 GGCGCC G[GA]^CG[CT]C
AfaI 126 GTAC GT^AC
AhdI 78 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 441 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 78 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 380 TGGCCA TGG^CCA
BamHI 307 GGATCC G^GATCC
BanI 465 GGCGCC G^G[CT][GA]CC
BanII 178 GAGCCC G[GA]GC[CT]^C
BbeI 465 GGCGCC GGCGC^C
BmyI 178 GAGCCC G[AGT]GC[ACT]^C
BsaHI 465 GGCGCC G[GA]^CG[CT]C
BseAI 174 TCCGGA T^CCGGA
BseRI 325 CTCCTC GAGGAG(10/8)
BsmI 359 GCATTC GAATGC(1/-1)
Bsp1286I 178 GAGCCC G[AGT]GC[ACT]^C
BspEI 174 TCCGGA T^CCGGA
BsrBI 115 GAGCGG CCGCTC(-3/-3)
BsrFI 233 ACCGGC [GA]^CCGG[CT]
BstAPI 482 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 483 CATCC GGATG(2/0)
BstUI 265 CGCG CG^CG
BstYI 307 GGATCC [GA]^GATC[CT]
CfoI 466 GCGC GCG^C
Cfr10I 233 ACCGGC [GA]^CCGG[CT]
EaeI 380 TGGCCA [CT]^GGCC[GA]
Eam1105I 78 GACCCCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 30 CCTCCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 270 CCCGC CCCGC(4/6)
FokI 483 CATCC GGATG(9/13)
HaeII 465 GGCGCC [GA]GCGC^[CT]
HgaI 263 GACGC GACGC(5/10)
HhaI 466 GCGC GCG^C
Hin1I 465 GGCGCC G[GA]^CG[CT]C
HinP1I 466 GCGC G^CGC
HphI 302 TCACC GGTGA(8/7)
KasI 465 GGCGCC G^GCGCC
MfeI 503 CAATTG C^AATTG
MflI 307 GGATCC [GA]^GATC[CT]
MluNI 380 TGGCCA TGG^CCA
MroI 174 TCCGGA T^CCGGA
MscI 380 TGGCCA TGG^CCA
MspA1I 151 CAGCTG C[AC]G^C[GT]G
MunI 503 CAATTG C^AATTG
MvnI 265 CGCG CG^CG
NarI 465 GGCGCC GG^CGCC
NlaIII 215 CATG CATG^
PflMI 440 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 151 CAGCTG CAG^CTG
RsaI 126 GTAC GT^AC
SfoI 465 GGCGCC GGC^GCC
SgrAI 232 CACCGGCG C[GA]^CCGG[CT]G
Tsp509I 504 AATT ^AATT
TspRI 445 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 440 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 307 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 49 GGACC G^G[AT]CC
186 GGTCC
BbvI 228 GCAGC GCAGC(8/12)
496 GCAGC
BsmAI 261 GAGAC GTCTC(1/5)
388 GAGAC
BsmBI 261 GAGACG CGTCTC(1/5)
388 GAGACG
BsmFI 48 GGGAC GGGAC(10/14)
450 GGGAC
BsrDI 135 CATTGC GCAATG(2/0)
491 GCAATG
BsrI 446 ACTGG ACTGG(1/-1)
219 CCAGT
EarI 373 GAAGAG CTCTTC(1/4)
434 GAAGAG
Ksp632I 373 GAAGAG CTCTTC(1/4)
434 GAAGAG
MboII 373 GAAGA GAAGA(8/7)
434 GAAGA
PleI 84 GAGTC GAGTC(4/5)
403 GAGTC
SapI 373 GAAGAGC GCTCTTC(1/4)
434 GAAGAGC
SmaI 129 CCCGGG CCC^GGG
256 CCCGGG
TfiI 411 GATTC G^A[AT]TC
429 GAATC
TseI 228 GCAGC G^C[AT]GC
496 GCAGC
XcmI 143 CCAAAGAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
368 CCAGGGAAGAGCTGG
XmaCI 129 CCCGGG C^CCGGG
256 CCCGGG
XmaI 129 CCCGGG C^CCGGG
256 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI AlwI AvaI BcnI
BsaJI BsaWI BsiYI BslI BsoBI
BssKI BstNI Cac8I Cfr13I DpnII
EcoRII Fnu4HI HaeIII HapII HinfI
HpaII ItaI MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIV
Sau3AI Sau96I ScrFI SfaNI TaqI
TthHB8I