Restriction map for
pFN21AE0593
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# NO CUT ENZYMES ( 162 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BanI
BbeI BbrPI BbsI BbvCI BcgI
BclI BcnI BfaI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HapII HgaI HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI KspI MaeI MaeIII
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tsp45I Tsp509I Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 585 GACGTC GACGT^C
AcyI 585 GACGTC G[GA]^CG[CT]C
AfaI 466 GTAC GT^AC
ApaI 374 GGGCCC GGGCC^C
Asp700I 647 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 398 GGACC G^G[AT]CC
BamHI 227 GGATCC G^GATCC
BciVI 194 GTATCC GTATCC(6/5)
BsaBI 228 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 585 GACGTC G[GA]^CG[CT]C
BseRI 116 CTCCTC GAGGAG(10/8)
BsiEI 173 CGGCCG CG[GA][CT]^CG
BsmAI 405 GAGAC GTCTC(1/5)
BsmFI 707 GGGAC GGGAC(10/14)
BsrI 419 ACTGG ACTGG(1/-1)
BstYI 227 GGATCC [GA]^GATC[CT]
EaeI 173 CGGCCG [CT]^GGCC[GA]
EagI 173 CGGCCG C^GGCCG
EarI 439 GAAGAG CTCTTC(1/4)
EclXI 173 CGGCCG C^GGCCG
Eco52I 173 CGGCCG C^GGCCG
EcoT14I 702 CCAAGG C^C[AT][AT]GG
FauI 639 CCCGC CCCGC(4/6)
HaeII 142 GGCGCT [GA]GCGC^[CT]
Hin1I 585 GACGTC G[GA]^CG[CT]C
Ksp632I 439 GAAGAG CTCTTC(1/4)
MamI 228 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 227 GGATCC [GA]^GATC[CT]
MseI 416 TTAA T^TAA
NlaIII 684 CATG CATG^
PflMI 364 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 466 GTAC GT^AC
SfaNI 44 GATGC GCATC(5/9)
StyI 702 CCAAGG C^C[AT][AT]GG
TaqI 645 TCGA T^CGA
Tru9I 416 TTAA T^TAA
TthHB8I 645 TCGA T^CGA
Van91I 364 CCAACAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 522 CCAGAACTCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 227 GGATCC [GA]^GATC[CT]
XmnI 647 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccII 171 CGCG CG^CG
463 CGCG
AciI 640 CCGC CCGC(-3/-1)
172 GCGG
AlwI 228 GATCC GGATC(4/5)
227 GGATC
AlwNI 140 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
485 CAGCACCTG
AocI 160 CCTAAGG CC^T[ACGT]AGG
313 CCTCAGG
BpmI 626 CTCCAG CTGGAG(16/14)
402 CTGGAG
BsgI 200 CTGCAC GTGCAG(16/14)
571 CTGCAC
BspMI 79 ACCTGC ACCTGC(4/8)
335 GCAGGT
BstUI 171 CGCG CG^CG
463 CGCG
BstXI 24 CCAGCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
331 CCAGGCAGGTGG
Bsu36I 160 CCTAAGG CC^T[ACGT]AGG
313 CCTCAGG
DpnII 228 GATC ^GATC
281 GATC
Eco81I 160 CCTAAGG CC^T[ACGT]AGG
313 CCTCAGG
HphI 77 TCACC GGTGA(8/7)
554 TCACC
MaeII 563 ACGT A^CGT
586 ACGT
MboI 228 GATC ^GATC
281 GATC
MslI 682 CACATGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
684 CATGGGTGTG
MvnI 171 CGCG CG^CG
463 CGCG
NdeII 228 GATC ^GATC
281 GATC
Sau3AI 228 GATC ^GATC
281 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI BanII BbvI BglI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoNI EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
ItaI MboII MnlI MvaI MwoI
NlaIV Sau96I ScrFI TseI TspRI