Restriction map for
pFN21AE0593
(Linear) (Six-base) MAPSORT of: KIEE0593.seq Check: 8981 from: 1 to: 741
KIEE0593.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 88 326 741
Size: 88 238 415
Fragments arranged by size:
415 238 88
AatII G_ACGT'C
Cuts at: 0 589 741
Size: 589 152
AleI CACnn'nnGTG
Cuts at: 0 686 741
Size: 686 55
AlwNI CAG_nnn'CTG
Cuts at: 0 145 490 741
Size: 145 345 251
Fragments arranged by size:
345 251 145
ApaI G_GGCC'C
Cuts at: 0 378 741
Size: 378 363
BamHI G'GATC_C
Cuts at: 0 227 741
Size: 227 514
BanII G_rGCy'C
Cuts at: 0 103 378 627 741
Size: 103 275 249 114
Fragments arranged by size:
275 249 114 103
BciVI GTATCCnnnnn_n'
Cuts at: 0 205 741
Size: 205 536
BglI GCCn_nnn'nGGC
Cuts at: 0 181 265 622 741
Size: 181 84 357 119
Fragments arranged by size:
357 181 119 84
Bme1580I G_kGCm'C
Cuts at: 0 7 38 297 378 479 741
Size: 7 31 259 81 101 262
Fragments arranged by size:
262 259 101 81 31 7
BmgBI CAC'GTC
Cuts at: 0 564 741
Size: 564 177
BmrI ACTGGGnnnn_n'
Cuts at: 0 429 741
Size: 429 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 611 741
Size: 423 188 130
Fragments arranged by size:
423 188 130
BsaBI GATnn'nnATC
Cuts at: 0 232 741
Size: 232 509
BsaHI Gr'CG_yC
Cuts at: 0 586 741
Size: 586 155
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 527 557 741
Size: 527 30 184
Fragments arranged by size:
527 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 107 741
Size: 107 634
BseYI C'CCAG_C
Cuts at: 0 31 129 741
Size: 31 98 612
Fragments arranged by size:
612 98 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 556 741
Size: 185 371 185
Fragments arranged by size:
371 185 185
BsiEI CG_ry'CG
Cuts at: 0 176 741
Size: 176 565
Bsp1286I G_dGCh'C
Cuts at: 0 7 38 103 297 378 479 627 741
Size: 7 31 65 194 81 101 148 114
Fragments arranged by size:
194 148 114 101 81 65 31 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 88 326 741
Size: 88 238 415
Fragments arranged by size:
415 238 88
BstXI CCAn_nnnn'nTGG
Cuts at: 0 31 338 741
Size: 31 307 403
Fragments arranged by size:
403 307 31
BstYI r'GATC_y
Cuts at: 0 227 741
Size: 227 514
Bsu36I CC'TnA_GG
Cuts at: 0 161 314 741
Size: 161 153 427
Fragments arranged by size:
427 161 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 57 741
Size: 57 684
EaeI y'GGCC_r
Cuts at: 0 173 741
Size: 173 568
EagI C'GGCC_G
Cuts at: 0 173 741
Size: 173 568
EarI CTCTTCn'nnn_
Cuts at: 0 434 741
Size: 434 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 611 741
Size: 423 188 130
Fragments arranged by size:
423 188 130
EcoNI CCTnn'n_nnAGG
Cuts at: 0 122 301 313 741
Size: 122 179 12 428
Fragments arranged by size:
428 179 122 12
EcoO109I rG'GnC_Cy
Cuts at: 0 306 323 374 741
Size: 306 17 51 367
Fragments arranged by size:
367 306 51 17
HaeII r_GCGC'y
Cuts at: 0 146 741
Size: 146 595
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 102 134 527 559 741
Size: 102 32 393 32 182
Fragments arranged by size:
393 182 102 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 741
Size: 388 353
MslI CAynn'nnrTG
Cuts at: 0 686 688 741
Size: 686 2 53
Fragments arranged by size:
686 53 2
PasI CC'CwG_GG
Cuts at: 0 262 741
Size: 262 479
PflMI CCAn_nnn'nTGG
Cuts at: 0 370 741
Size: 370 371
PspOMI G'GGCC_C
Cuts at: 0 374 741
Size: 374 367
StyI C'CwwG_G
Cuts at: 0 702 741
Size: 702 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 530 676 741
Size: 530 146 65
Fragments arranged by size:
530 146 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 529 741
Size: 529 212
XmnI GAAnn'nnTTC
Cuts at: 0 651 741
Size: 651 90
ZraI GAC'GTC
Cuts at: 0 587 741
Size: 587 154
Enzymes that do cut:
AarI AatII AleI AlwNI ApaI BamHI BanII BciVI
BglI Bme1580I BmgBI BmrI BpmI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI Bsp1286I BspMI BstXI BstYI
Bsu36I BtgZI EaeI EagI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I MmeI MslI PasI PflMI PspOMI StyI
TaqII XcmI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BbvCI BcgI
BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI