(Circular) (Six-base) MAPSORT of: pFN21AE0585.seq Check: 3229 from: 1 to: 5611 pFN21AE0585 5611 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2859 3421 278 Size: 53 83 186 936 1323 562 2468 Fragments arranged by size: 2468 1323 936 562 186 83 53 AccI GT'mk_AC Cuts at: 1937 2677 2954 1937 Size: 740 277 4594 Fragments arranged by size: 4594 740 277 Acc65I G'GTAC_C Cuts at: 2932 2932 Size: 5611 AclI AA'CG_TT Cuts at: 1563 3738 4111 1563 Size: 2175 373 3063 Fragments arranged by size: 3063 2175 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2766 3674 5058 5326 1632 Size: 1134 908 1384 268 1917 Fragments arranged by size: 1917 1384 1134 908 268 AflII C'TTAA_G Cuts at: 820 1017 2261 820 Size: 197 1244 4170 Fragments arranged by size: 4170 1244 197 AflIII A'CryG_T Cuts at: 1203 2610 4510 5487 1203 Size: 1407 1900 977 1327 Fragments arranged by size: 1900 1407 1327 977 AgeI A'CCGG_T Cuts at: 4442 4442 Size: 5611 AhdI GACnn_n'nnGTC Cuts at: 2662 2855 4340 2662 Size: 193 1485 3933 Fragments arranged by size: 3933 1485 193 AleI CACnn'nnGTG Cuts at: 1166 2522 1166 Size: 1356 4255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5611 AlwNI CAG_nnn'CTG Cuts at: 2351 2519 3354 4926 2351 Size: 168 835 1572 3036 Fragments arranged by size: 3036 1572 835 168 ApaLI G'TGCA_C Cuts at: 3668 4824 3668 Size: 1156 4455 ApoI r'AATT_y Cuts at: 1493 2728 2920 3146 3298 5407 1493 Size: 1235 192 226 152 2109 1697 Fragments arranged by size: 2109 1697 1235 226 192 152 AseI AT'TA_AT Cuts at: 160 1032 4165 160 Size: 872 3133 1606 Fragments arranged by size: 3133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 AvaI C'yCGr_G Cuts at: 1870 1943 2936 1870 Size: 73 993 4545 Fragments arranged by size: 4545 993 73 BamHI G'GATC_C Cuts at: 2941 3308 2941 Size: 367 5244 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2932 4387 618 Size: 325 217 627 1145 1455 1842 Fragments arranged by size: 1842 1455 1145 627 325 217 BanII G_rGCy'C Cuts at: 1391 2930 1391 Size: 1539 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4640 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3802 3836 1903 Size: 34 1865 34 3678 Fragments arranged by size: 3678 1865 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 3503 4713 2633 Size: 870 1210 3531 Fragments arranged by size: 3531 1210 870 BclI T'GATC_A Cuts at: 1548 2370 1548 Size: 822 4789 BfrBI ATG'CAT Cuts at: 2300 2461 2479 2890 2894 2300 Size: 161 18 411 4 5017 Fragments arranged by size: 5017 411 161 18 4 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4222 136 Size: 107 122 71 631 605 2550 1525 Fragments arranged by size: 2550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2254 2539 5606 2254 Size: 285 3067 2259 Fragments arranged by size: 3067 2259 285 BlpI GC'TnA_GC Cuts at: 3028 3028 Size: 5611 Bme1580I G_kGCm'C Cuts at: 1790 3672 4828 1790 Size: 1882 1156 2573 Fragments arranged by size: 2573 1882 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5611 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2529 3342 4300 Size: 416 53 83 399 24 1112 813 958 Cuts at: 4300 442 Size: 1753 Fragments arranged by size: 1753 1112 958 813 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5611 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2601 4271 1239 Size: 1362 1670 2579 Fragments arranged by size: 2579 1670 1362 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3736 4601 4899 3736 Size: 865 298 4448 Fragments arranged by size: 4448 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2670 4274 882 Size: 617 1171 1604 2219 Fragments arranged by size: 2219 1604 1171 617 BsaAI yAC'GTr Cuts at: 493 1204 2231 3402 493 Size: 711 1027 1171 2702 Fragments arranged by size: 2702 1171 1027 711 BsaBI GATnn'nnATC Cuts at: 5605 5605 Size: 5611 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2856 3418 3800 4435 Size: 53 83 186 936 1323 562 382 635 Cuts at: 4435 275 Size: 1451 Fragments arranged by size: 1451 1323 936 635 562 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2074 4044 4442 4716 4863 5274 2074 Size: 1970 398 274 147 411 2411 Fragments arranged by size: 2411 1970 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2166 2196 1178 Size: 30 165 30 763 30 4593 Fragments arranged by size: 4593 763 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5611 BseYI C'CCAG_C Cuts at: 4814 5310 4814 Size: 496 5115 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 2565 1163 Size: 573 483 346 4209 Fragments arranged by size: 4209 573 483 346 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2075 3061 3822 4850 1540 Size: 275 186 74 986 761 1028 2301 Fragments arranged by size: 2301 1028 986 761 275 186 74 BsiHKAI G_wGCw'C Cuts at: 3672 3757 4828 3672 Size: 85 1071 4455 Fragments arranged by size: 4455 1071 85 BsmI GAATG_Cn' Cuts at: 2031 2461 3122 3215 2031 Size: 430 661 93 4427 Fragments arranged by size: 4427 661 430 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2930 3672 3757 4828 1391 Size: 399 1140 742 85 1071 2174 Fragments arranged by size: 2174 1140 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5274 5274 Size: 5611 BspHI T'CATG_A Cuts at: 3500 5230 3500 Size: 1730 3881 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2966 844 Size: 2122 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3498 1394 Size: 429 1675 3507 Fragments arranged by size: 3507 1675 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4106 4280 65 Size: 1183 2858 174 1396 Fragments arranged by size: 2858 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2553 4255 4442 1700 Size: 110 743 1702 187 2869 Fragments arranged by size: 2869 1702 743 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5611 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5611 BssSI C'ACGA_G Cuts at: 3671 4683 3671 Size: 1012 4599 BstAPI GCAn_nnn'nTGC Cuts at: 794 2900 794 Size: 2106 3505 BstBI TT'CG_AA Cuts at: 2107 4415 2107 Size: 2308 3303 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5611 BstXI CCAn_nnnn'nTGG Cuts at: 2524 2524 Size: 5611 BstYI r'GATC_y Cuts at: 1972 2254 2539 2836 2941 3308 3693 3710 5162 Size: 282 285 297 105 367 385 17 1452 Cuts at: 5162 5248 5260 5606 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 297 285 282 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4120 Fragments arranged by size: 4120 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4872 Fragments arranged by size: 4872 683 56 BtsI GCAGTG_nn' Cuts at: 2449 2898 3127 3921 3941 2449 Size: 449 229 794 20 4119 Fragments arranged by size: 4119 794 449 229 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5611 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5576 DraI TTT'AAA Cuts at: 2190 2915 2190 Size: 725 4886 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4618 809 Size: 507 3302 1802 Fragments arranged by size: 3302 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2551 3058 3947 Size: 54 1425 50 275 18 721 507 889 Cuts at: 3947 5576 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 721 507 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3058 1537 Size: 275 1246 4090 Fragments arranged by size: 4090 1246 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2464 2781 3541 1353 Size: 1111 317 760 3423 Fragments arranged by size: 3423 1111 760 317 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4198 4572 4718 387 Size: 1064 138 2609 374 146 1280 Fragments arranged by size: 2609 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2601 2766 3674 4271 5058 5326 1239 Size: 393 969 165 908 597 787 268 1524 Fragments arranged by size: 1524 969 908 787 597 393 268 165 EcoRI G'AATT_C Cuts at: 2920 3298 2920 Size: 378 5233 FspI TGC'GCA Cuts at: 4117 4117 Size: 5611 HaeII r_GCGC'y Cuts at: 1678 4758 1678 Size: 3080 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2648 2680 2841 Size: 32 135 32 428 32 616 32 161 Cuts at: 2841 2873 4252 4284 4326 4358 1373 Size: 32 1379 32 42 32 2626 Fragments arranged by size: 2626 1379 616 428 161 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2955 3201 669 Size: 988 281 1017 246 3079 Fragments arranged by size: 3079 1017 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5611 HpaI GTT'AAC Cuts at: 3201 3201 Size: 5611 KpnI G_GTAC'C Cuts at: 2936 2936 Size: 5611 MfeI C'AATT_G Cuts at: 3210 3210 Size: 5611 MluI A'CGCG_T Cuts at: 5487 5487 Size: 5611 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2077 2830 4725 4909 1305 Size: 772 753 1895 184 2007 Fragments arranged by size: 2007 1895 772 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3789 Fragments arranged by size: 3789 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2280 2522 2634 3569 3928 4087 518 Size: 648 1114 242 112 935 359 159 2042 Fragments arranged by size: 2042 1114 935 648 359 242 159 112 MspA1I CmG'CkG Cuts at: 1606 2579 3027 3704 4852 5097 1606 Size: 973 448 677 1148 245 2120 Fragments arranged by size: 2120 1148 973 677 448 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4120 Fragments arranged by size: 4120 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5611 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5611 NotI GC'GGCC_GC Cuts at: 3058 3058 Size: 5611 NsiI A_TGCA'T Cuts at: 2302 2463 2481 2892 2896 2302 Size: 161 18 411 4 5017 Fragments arranged by size: 5017 411 161 18 4 NspI r_CATG'y Cuts at: 1132 1229 2461 2614 2894 2898 2969 4514 1132 Size: 97 1232 153 280 4 71 1545 2229 Fragments arranged by size: 2229 1545 1232 280 153 97 71 4 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5611 PciI A'CATG_T Cuts at: 2610 4510 2610 Size: 1900 3711 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5611 PmeI GTTT'AAAC Cuts at: 2915 2915 Size: 5611 PmlI CAC'GTG Cuts at: 2231 2231 Size: 5611 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3680 3712 5193 5225 3680 Size: 32 1481 32 4066 Fragments arranged by size: 4066 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5611 PsiI TTA'TAA Cuts at: 3181 3181 Size: 5611 PstI C_TGCA'G Cuts at: 830 2963 830 Size: 2133 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5611 PvuII CAG'CTG Cuts at: 2579 2579 Size: 5611 SalI G'TCGA_C Cuts at: 1936 2953 1936 Size: 1017 4594 SbfI CC_TGCA'GG Cuts at: 2963 2963 Size: 5611 ScaI AGT'ACT Cuts at: 3859 5571 3859 Size: 1712 3899 SfcI C'TryA_G Cuts at: 826 1045 2959 4094 4775 4966 826 Size: 219 1914 1135 681 191 1471 Fragments arranged by size: 1914 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5611 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5611 SmaI CCC'GGG Cuts at: 1872 2938 1872 Size: 1066 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 3715 4616 4878 820 Size: 197 926 318 1454 901 262 1553 Fragments arranged by size: 1553 1454 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 3402 493 Size: 2909 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5611 SphI G_CATG'C Cuts at: 1132 2894 2898 2969 1132 Size: 1762 4 71 3774 Fragments arranged by size: 3774 1762 71 4 SspI AAT'ATT Cuts at: 5 52 2294 3535 5 Size: 47 2242 1241 2081 Fragments arranged by size: 2242 2081 1241 47 StuI AGG'CCT Cuts at: 1517 2066 1517 Size: 549 5062 StyI C'CwwG_G Cuts at: 513 1065 2004 3050 513 Size: 552 939 1046 3074 Fragments arranged by size: 3074 1046 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3638 3655 3808 3993 4438 325 Size: 1255 2058 17 153 185 445 1498 Fragments arranged by size: 2058 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3857 5569 96 Size: 274 80 33 51 1443 1880 1712 138 Fragments arranged by size: 1880 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5571 5603 5571 Size: 32 5579 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5611 XbaI T'CTAG_A Cuts at: 2947 2947 Size: 5611 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5611 XmaI C'CCGG_G Cuts at: 1870 2936 1870 Size: 1066 4545 XmnI GAAnn'nnTTC Cuts at: 3740 3740 Size: 5611 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2857 3419 276 Size: 53 83 186 936 1323 562 2468 Fragments arranged by size: 2468 1323 936 562 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI