Restriction map for
pFN21AE0581
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AviII AvrII BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BseAI BseRI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp509I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccII 682 CGCG CG^CG
Aor51HI 45 AGCGCT AGC^GCT
ApaI 435 GGGCCC GGGCC^C
AspI 142 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 269 GGATCC G^GATCC
BbsI 97 GTCTTC GAAGAC(2/6)
BclI 608 TGATCA T^GATCA
BfaI 155 CTAG C^TAG
BlpI 642 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 642 GCTCAGC GC^T[ACGT]AGC
BpuAI 97 GTCTTC GAAGAC(2/6)
BsaI 418 GAGACC GGTCTC(1/5)
BsaWI 191 ACCGGA [AT]^CCGG[AT]
BspDI 160 ATCGAT AT^CGAT
BspMI 421 ACCTGC ACCTGC(4/8)
BsrFI 665 ACCGGC [GA]^CCGG[CT]
BssHII 681 GCGCGC G^CGCGC
BstAPI 618 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 682 CGCG CG^CG
CelII 642 GCTCAGC GC^T[ACGT]AGC
Cfr10I 665 ACCGGC [GA]^CCGG[CT]
ClaI 160 ATCGAT AT^CGAT
DsaI 59 CCATGG C^C[GA][CT]GG
Eco47III 45 AGCGCT AGC^GCT
EcoNI 345 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 59 CCATGG C^C[AT][AT]GG
FbaI 608 TGATCA T^GATCA
MaeI 155 CTAG C^TAG
MaeII 527 ACGT A^CGT
MboII 98 TCTTC GAAGA(8/7)
MvnI 682 CGCG CG^CG
NcoI 59 CCATGG C^CATGG
PflFI 142 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 788 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 616 CTGCAG CTGCA^G
PvuII 327 CAGCTG CAG^CTG
SacI 334 GAGCTC GAGCT^C
SfcI 616 CTGCAG C^T[GA][CT]AG
SphI 635 GCATGC GCATG^C
StyI 59 CCATGG C^C[AT][AT]GG
TspRI 628 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 142 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 619 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
817 CAGCCGCTG
AspHI 181 GTGCTC G[AT]GC[AT]^C
334 GAGCTC
BalI 530 TGGCCA TGG^CCA
581 TGGCCA
BsiHKAI 181 GTGCTC G[AT]GC[AT]^C
334 GAGCTC
BsmAI 250 GAGAC GTCTC(1/5)
418 GAGAC
BsrI 629 ACTGG ACTGG(1/-1)
303 CCAGT
BstXI 569 CCACCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
752 CCAGCGCCCTGG
BstYI 269 GGATCC [GA]^GATC[CT]
359 AGATCC
DdeI 643 CTCAG C^T[ACGT]AG
727 CTGAG
DraII 435 GGGCCCC [GA]G^G[ACGT]CC[CT]
788 AGGTCCT
EaeI 530 TGGCCA [CT]^GGCC[GA]
581 TGGCCA
EcoO109I 435 GGGCCCC [GA]G^G[ACGT]CC[CT]
788 AGGTCCT
HaeII 45 AGCGCT [GA]GCGC^[CT]
754 AGCGCC
HgaI 621 GACGC GACGC(5/10)
494 GCGTC
MaeIII 17 GTGAC ^GT[ACGT]AC
496 GTCAC
MflI 269 GGATCC [GA]^GATC[CT]
359 AGATCC
MluNI 530 TGGCCA TGG^CCA
581 TGGCCA
MscI 530 TGGCCA TGG^CCA
581 TGGCCA
MspA1I 327 CAGCTG C[AC]G^C[GT]G
820 CCGCTG
NspI 320 GCATGT [GA]CATG^[CT]
635 GCATGC
Tsp45I 17 GTGAC ^GT[GC]AC
496 GTCAC
XcmI 569 CCACCTGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
662 CCAACCGGCCCCTGG
XhoII 269 GGATCC [GA]^GATC[CT]
359 AGATCC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsgI BsiYI BslI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MboI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI TthHB8I