Restriction map for
pFN21AE0581
(Linear) (Six-base) MAPSORT of: KIEE0581.seq Check: 1893 from: 1 to: 849
KIEE0581.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AfeI AGC'GCT
Cuts at: 0 47 849
Size: 47 802
AleI CACnn'nnGTG
Cuts at: 0 430 849
Size: 430 419
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 442 474 849
Size: 442 32 375
Fragments arranged by size:
442 375 32
AlwNI CAG_nnn'CTG
Cuts at: 0 624 822 849
Size: 624 198 27
Fragments arranged by size:
624 198 27
ApaI G_GGCC'C
Cuts at: 0 439 849
Size: 439 410
BamHI G'GATC_C
Cuts at: 0 269 849
Size: 269 580
BanII G_rGCy'C
Cuts at: 0 338 439 701 743 849
Size: 338 101 262 42 106
Fragments arranged by size:
338 262 106 101 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 90 849
Size: 90 759
BclI T'GATC_A
Cuts at: 0 608 849
Size: 608 241
BlpI GC'TnA_GC
Cuts at: 0 643 849
Size: 643 206
Bme1580I G_kGCm'C
Cuts at: 0 439 512 849
Size: 439 73 337
Fragments arranged by size:
439 337 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 156 331 849
Size: 55 101 175 518
Fragments arranged by size:
518 175 101 55
Bpu10I CC'TnA_GC
Cuts at: 0 727 849
Size: 727 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 412 849
Size: 412 437
BsaWI w'CCGG_w
Cuts at: 0 191 849
Size: 191 658
BseYI C'CCAG_C
Cuts at: 0 329 490 751 849
Size: 329 161 261 98
Fragments arranged by size:
329 261 161 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 408 610 849
Size: 300 108 202 239
Fragments arranged by size:
300 239 202 108
BsiHKAI G_wGCw'C
Cuts at: 0 185 338 849
Size: 185 153 511
Fragments arranged by size:
511 185 153
Bsp1286I G_dGCh'C
Cuts at: 0 185 338 439 512 701 743 849
Size: 185 153 101 73 189 42 106
Fragments arranged by size:
189 185 153 106 101 73 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 430 849
Size: 430 419
BsrFI r'CCGG_y
Cuts at: 0 665 849
Size: 665 184
BssHII G'CGCG_C
Cuts at: 0 681 849
Size: 681 168
BstAPI GCAn_nnn'nTGC
Cuts at: 0 624 849
Size: 624 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 759 849
Size: 576 183 90
Fragments arranged by size:
576 183 90
BstYI r'GATC_y
Cuts at: 0 269 359 849
Size: 269 90 490
Fragments arranged by size:
490 269 90
BtgI C'CryG_G
Cuts at: 0 59 849
Size: 59 790
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 687 849
Size: 687 162
ClaI AT'CG_AT
Cuts at: 0 161 849
Size: 161 688
EaeI y'GGCC_r
Cuts at: 0 530 581 849
Size: 530 51 268
Fragments arranged by size:
530 268 51
EcoICRI GAG'CTC
Cuts at: 0 336 849
Size: 336 513
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 156 331 849
Size: 55 101 175 518
Fragments arranged by size:
518 175 101 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 849
Size: 349 500
EcoO109I rG'GnC_Cy
Cuts at: 0 436 789 849
Size: 436 353 60
Fragments arranged by size:
436 353 60
HaeII r_GCGC'y
Cuts at: 0 49 758 849
Size: 49 709 91
Fragments arranged by size:
709 91 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 849
Size: 154 32 663
Fragments arranged by size:
663 154 32
MscI TGG'CCA
Cuts at: 0 532 583 849
Size: 532 51 266
Fragments arranged by size:
532 266 51
MslI CAynn'nnrTG
Cuts at: 0 430 469 652 849
Size: 430 39 183 197
Fragments arranged by size:
430 197 183 39
MspA1I CmG'CkG
Cuts at: 0 329 822 849
Size: 329 493 27
Fragments arranged by size:
493 329 27
NcoI C'CATG_G
Cuts at: 0 59 849
Size: 59 790
NspI r_CATG'y
Cuts at: 0 324 639 849
Size: 324 315 210
Fragments arranged by size:
324 315 210
PasI CC'CwG_GG
Cuts at: 0 672 849
Size: 672 177
PfoI T'CCnGG_A
Cuts at: 0 101 347 849
Size: 101 246 502
Fragments arranged by size:
502 246 101
PpuMI rG'GwC_Cy
Cuts at: 0 789 849
Size: 789 60
PspOMI G'GGCC_C
Cuts at: 0 435 849
Size: 435 414
PstI C_TGCA'G
Cuts at: 0 620 849
Size: 620 229
PvuII CAG'CTG
Cuts at: 0 329 849
Size: 329 520
SacI G_AGCT'C
Cuts at: 0 338 849
Size: 338 511
SfcI C'TryA_G
Cuts at: 0 616 849
Size: 616 233
SphI G_CATG'C
Cuts at: 0 639 849
Size: 639 210
StyI C'CwwG_G
Cuts at: 0 59 849
Size: 59 790
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 188 220 849
Size: 188 32 629
Fragments arranged by size:
629 188 32
Tth111I GACn'n_nGTC
Cuts at: 0 145 849
Size: 145 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 576 669 849
Size: 576 93 180
Fragments arranged by size:
576 180 93
Enzymes that do cut:
AfeI AleI AloI AlwNI ApaI BamHI BanII BbsI
BclI BlpI Bme1580I BpmI Bpu10I BsaI BsaWI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BstAPI BstXI
BstYI BtgI BtgZI ClaI EaeI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MscI MslI MspA1I NcoI NspI
PasI PfoI PpuMI PspOMI PstI PvuII SacI SfcI
SphI StyI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssSI BstBI
BstEII BstZ17I Bsu36I BtsI CspCI DraI DraIII DrdI
EagI EarI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI XbaI XhoI XmaI XmnI ZraI