Restriction map for
pFN21AE0577
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
BalI BamHI BanII BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BpmI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
FauI FbaI FseI FspI HapII
HincII HindII HindIII HpaI HpaII
KpnI KspI MamI MfeI MflI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcyI 730 GGCGCC G[GA]^CG[CT]C
Aor51HI 427 AGCGCT AGC^GCT
AspHI 667 GTGCTC G[AT]GC[AT]^C
AvaII 577 GGTCC G^G[AT]CC
AvrII 241 CCTAGG C^CTAGG
BanI 730 GGCGCC G^G[CT][GA]CC
BbeI 730 GGCGCC GGCGC^C
BbsI 808 GAAGAC GAAGAC(2/6)
BbvI 72 GCTGC GCAGC(8/12)
BlnI 241 CCTAGG C^CTAGG
BlpI 800 GCTTAGC GC^T[ACGT]AGC
BmyI 667 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 800 GCTTAGC GC^T[ACGT]AGC
BpuAI 808 GAAGAC GAAGAC(2/6)
BsaHI 730 GGCGCC G[GA]^CG[CT]C
BsgI 73 CTGCAC GTGCAG(16/14)
BsiHKAI 667 GTGCTC G[AT]GC[AT]^C
BsiYI 790 CCTGCATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 790 CCTGCATCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 748 GTCTC GTCTC(1/5)
BsmI 337 GAATGC GAATGC(1/-1)
Bsp1286I 667 GTGCTC G[AGT]GC[ACT]^C
BspMI 512 GCAGGT ACCTGC(4/8)
BssKI 348 CCTGG ^CC[ACGT]GG
BssSI 662 CTCGTG CACGAG(-5/-1)
BstNI 348 CCTGG CC^[AT]GG
Cac8I 900 GCATGC GC[ACGT]^[ACGT]GC
CelII 800 GCTTAGC GC^T[ACGT]AGC
Cfr13I 577 GGTCC G^G[ACGT]CC
Eco47III 427 AGCGCT AGC^GCT
EcoRII 348 CCTGG ^CC[AT]GG
EcoRV 279 GATATC GAT^ATC
HaeIII 360 GGCC GG^CC
HgaI 786 GCGTC GACGC(5/10)
Hin1I 730 GGCGCC G[GA]^CG[CT]C
HinfI 24 GACTC G^A[ACGT]TC
KasI 730 GGCGCC G^GCGCC
MaeII 77 ACGT A^CGT
MaeIII 31 GTGAC ^GT[ACGT]AC
MvaI 348 CCTGG CC^[AT]GG
MwoI 319 GCTTCCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 730 GGCGCC GG^CGCC
NlaIV 730 GGCGCC GG[ACGT]^[ACGT]CC
PleI 24 GACTC GAGTC(4/5)
PstI 510 CTGCAG CTGCA^G
Sau96I 577 GGTCC G^G[ACGT]CC
ScrFI 348 CCTGG CC^[ACGT]GG
SfoI 730 GGCGCC GGC^GCC
SmlI 879 CTTGAG C^T[CT][GA]AG
SphI 900 GCATGC GCATG^C
TaqI 63 TCGA T^CGA
TseI 72 GCTGC G^C[AT]GC
Tsp45I 31 GTGAC ^GT[GC]AC
TthHB8I 63 TCGA T^CGA
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 369 CCGC CCGC(-3/-1)
728 GCGG
AcsI 545 AAATTT [GA]^AATT[CT]
563 AAATTT
ApoI 545 AAATTT [GA]^AATT[CT]
563 AAATTT
BfaI 242 CTAG C^TAG
286 CTAG
BsrDI 274 GCAATG GCAATG(2/0)
572 GCAATG
BstF5I 469 CATCC GGATG(2/0)
245 GGATG
CfoI 428 GCGC GCG^C
731 GCGC
DpnII 61 GATC ^GATC
771 GATC
DsaI 67 CCATGG C^C[GA][CT]GG
119 CCATGG
EarI 82 CTCTTC CTCTTC(1/4)
911 GAAGAG
EcoT22I 898 ATGCAT ATGCA^T
902 ATGCAT
Fnu4HI 72 GCTGC GC^[ACGT]GC
728 GCGGC
FokI 469 CATCC GGATG(9/13)
245 GGATG
HaeII 427 AGCGCT [GA]GCGC^[CT]
730 GGCGCC
HhaI 428 GCGC GCG^C
731 GCGC
HinP1I 428 GCGC G^CGC
731 GCGC
ItaI 72 GCTGC GC^[ACGT]GC
728 GCGGC
Ksp632I 82 CTCTTC CTCTTC(1/4)
911 GAAGAG
MaeI 242 CTAG C^TAG
286 CTAG
MboI 61 GATC ^GATC
771 GATC
NcoI 67 CCATGG C^CATGG
119 CCATGG
NdeII 61 GATC ^GATC
771 GATC
NsiI 898 ATGCAT ATGCA^T
902 ATGCAT
NspI 724 ACATGC [GA]CATG^[CT]
900 GCATGC
Sau3AI 61 GATC ^GATC
771 GATC
SfcI 332 CTACAG C^T[GA][CT]AG
510 CTGCAG
TspRI 324 CAGTG CA[GC]TG[ACGT][ACGT]^
404 CAGTG
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
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AfaI AluI BsaJI BsrI DdeI
EcoT14I HphI MboII MnlI MseI
MslI NdeI NlaIII RsaI SfaNI
StyI Tru9I Tsp509I