(Circular) (Six-base) MAPSORT of: pFN21AE0577.seq Check: 4134 from: 1 to: 5632 pFN21AE0577 5632 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3442 278 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2975 1937 Size: 1038 4594 Acc65I G'GTAC_C Cuts at: 2953 2953 Size: 5632 AclI AA'CG_TT Cuts at: 1563 3759 4132 1563 Size: 2196 373 3063 Fragments arranged by size: 3063 2196 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2832 3695 5079 5347 1632 Size: 1200 863 1384 268 1917 Fragments arranged by size: 1917 1384 1200 863 268 AfeI AGC'GCT Cuts at: 2434 2434 Size: 5632 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5435 AflIII A'CryG_T Cuts at: 1203 4531 5508 1203 Size: 3328 977 1327 Fragments arranged by size: 3328 1327 977 AgeI A'CCGG_T Cuts at: 4463 4463 Size: 5632 AhdI GACnn_n'nnGTC Cuts at: 4361 4361 Size: 5632 AleI CACnn'nnGTG Cuts at: 1166 2126 1166 Size: 960 4672 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5632 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2133 2165 2133 Size: 32 5600 AlwNI CAG_nnn'CTG Cuts at: 3375 4947 3375 Size: 1572 4060 ApaLI G'TGCA_C Cuts at: 3689 4845 3689 Size: 1156 4476 ApoI r'AATT_y Cuts at: 1493 2550 2568 2941 3167 3319 5428 1493 Size: 1057 18 373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1057 373 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4186 160 Size: 872 3154 1606 Fragments arranged by size: 3154 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 AvaI C'yCGr_G Cuts at: 1870 1943 2957 1870 Size: 73 1014 4545 Fragments arranged by size: 4545 1014 73 AvrII C'CTAG_G Cuts at: 2246 2246 Size: 5632 BamHI G'GATC_C Cuts at: 2962 3329 2962 Size: 367 5265 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2735 2953 4408 618 Size: 325 217 627 948 218 1455 1842 Fragments arranged by size: 1842 1455 948 627 325 218 217 BanII G_rGCy'C Cuts at: 1391 2951 1391 Size: 1560 4072 BbeI G_GCGC'C Cuts at: 2739 2739 Size: 5632 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2820 928 Size: 971 921 3740 Fragments arranged by size: 3740 971 921 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3823 3857 1903 Size: 34 1886 34 3678 Fragments arranged by size: 3678 1886 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3524 4734 3524 Size: 1210 4422 BclI T'GATC_A Cuts at: 1548 1548 Size: 5632 BfrBI ATG'CAT Cuts at: 2905 2909 2905 Size: 4 5628 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4243 136 Size: 107 122 71 631 605 2571 1525 Fragments arranged by size: 2571 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5627 5627 Size: 5632 BlpI GC'TnA_GC Cuts at: 2806 3049 2806 Size: 243 5389 Bme1580I G_kGCm'C Cuts at: 1790 3693 4849 1790 Size: 1903 1156 2573 Fragments arranged by size: 2573 1903 1156 BmgBI CAC'GTC Cuts at: 1318 2083 1318 Size: 765 4867 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2302 3363 4321 Size: 416 53 83 399 24 885 1061 958 Cuts at: 4321 442 Size: 1753 Fragments arranged by size: 1753 1061 958 885 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5632 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4292 1239 Size: 3053 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2905 3757 4622 4920 2905 Size: 852 865 298 3617 Fragments arranged by size: 3617 865 852 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4295 882 Size: 617 2796 2219 Fragments arranged by size: 2796 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3423 493 Size: 711 2219 2702 Fragments arranged by size: 2702 2219 711 BsaBI GATnn'nnATC Cuts at: 5626 5626 Size: 5632 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2736 3439 3821 4456 Size: 53 83 186 936 1203 703 382 635 Cuts at: 4456 275 Size: 1451 Fragments arranged by size: 1451 1203 936 703 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4065 4463 4737 4884 5295 4065 Size: 398 274 147 411 4402 Fragments arranged by size: 4402 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5407 Fragments arranged by size: 5407 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5632 BseYI C'CCAG_C Cuts at: 4835 5331 4835 Size: 496 5136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 1163 Size: 573 327 4732 Fragments arranged by size: 4732 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3082 3843 4871 1540 Size: 275 186 1081 761 1028 2301 Fragments arranged by size: 2301 1081 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2676 3693 3778 4849 2676 Size: 1017 85 1071 3459 Fragments arranged by size: 3459 1071 1017 85 BsmI GAATG_Cn' Cuts at: 2348 3143 3236 2348 Size: 795 93 4744 Fragments arranged by size: 4744 795 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2676 2951 3693 3778 4849 1391 Size: 399 886 275 742 85 1071 2174 Fragments arranged by size: 2174 1071 886 742 399 275 85 BspEI T'CCGG_A Cuts at: 5295 5295 Size: 5632 BspHI T'CATG_A Cuts at: 3521 5251 3521 Size: 1730 3902 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2508 2987 844 Size: 1664 479 3489 Fragments arranged by size: 3489 1664 479 BsrBI CCG'CTC Cuts at: 1394 1823 3519 1394 Size: 429 1696 3507 Fragments arranged by size: 3507 1696 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2286 2584 4127 4301 65 Size: 1183 1038 298 1543 174 1396 Fragments arranged by size: 1543 1396 1183 1038 298 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4276 4463 1700 Size: 110 2466 187 2869 Fragments arranged by size: 2869 2466 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5632 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5632 BssSI C'ACGA_G Cuts at: 2667 3692 4704 2667 Size: 1025 1012 3595 Fragments arranged by size: 3595 1025 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5632 BstBI TT'CG_AA Cuts at: 4436 4436 Size: 5632 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5632 BstYI r'GATC_y Cuts at: 1972 2962 3329 3714 3731 5183 5269 5281 5627 Size: 990 367 385 17 1452 86 12 346 Cuts at: 5627 1972 Size: 1977 Fragments arranged by size: 1977 1452 990 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2072 2124 513 Size: 552 939 68 52 4021 Fragments arranged by size: 4021 939 552 68 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4893 Fragments arranged by size: 4893 683 56 BtsI GCAGTG_nn' Cuts at: 3148 3942 3962 3148 Size: 794 20 4818 Fragments arranged by size: 4818 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5597 DraI TTT'AAA Cuts at: 2936 2936 Size: 5632 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4639 809 Size: 507 3323 1802 Fragments arranged by size: 3323 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3079 3968 5597 Size: 54 1425 50 275 18 1249 889 1629 Cuts at: 5597 8 Size: 43 Fragments arranged by size: 1629 1425 1249 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3079 1537 Size: 275 1267 4090 Fragments arranged by size: 4090 1267 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2911 3562 1353 Size: 740 818 651 3423 Fragments arranged by size: 3423 818 740 651 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4219 4593 4739 387 Size: 1064 138 2630 374 146 1280 Fragments arranged by size: 2630 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2832 3695 4292 5079 5347 1239 Size: 393 1200 863 597 787 268 1524 Fragments arranged by size: 1524 1200 863 787 597 393 268 EcoRI G'AATT_C Cuts at: 2941 3319 2941 Size: 378 5254 EcoRV GAT'ATC Cuts at: 2286 2286 Size: 5632 FspI TGC'GCA Cuts at: 4138 4138 Size: 5632 HaeII r_GCGC'y Cuts at: 1678 2436 2739 4779 1678 Size: 758 303 2040 2531 Fragments arranged by size: 2531 2040 758 303 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4273 4305 4347 4379 1373 Size: 32 135 32 2701 32 42 32 2626 Fragments arranged by size: 2701 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2976 3222 669 Size: 988 281 1038 246 3079 Fragments arranged by size: 3079 1038 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5632 HpaI GTT'AAC Cuts at: 3222 3222 Size: 5632 KasI G'GCGC_C Cuts at: 2735 2735 Size: 5632 KpnI G_GTAC'C Cuts at: 2957 2957 Size: 5632 MfeI C'AATT_G Cuts at: 3231 3231 Size: 5632 MluI A'CGCG_T Cuts at: 5508 5508 Size: 5632 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2445 4746 4930 1305 Size: 1140 2301 184 2007 Fragments arranged by size: 2301 2007 1140 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3810 Fragments arranged by size: 3810 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2126 2642 2786 3590 3949 4108 Size: 648 957 3 516 144 804 359 159 Cuts at: 4108 518 Size: 2042 Fragments arranged by size: 2042 957 804 648 516 359 159 144 3 MspA1I CmG'CkG Cuts at: 1606 3048 3725 4873 5118 1606 Size: 1442 677 1148 245 2120 Fragments arranged by size: 2120 1442 1148 677 245 NarI GG'CG_CC Cuts at: 2736 2736 Size: 5632 NcoI C'CATG_G Cuts at: 513 1065 2004 2072 2124 513 Size: 552 939 68 52 4021 Fragments arranged by size: 4021 939 552 68 52 NdeI CA'TA_TG Cuts at: 387 2178 2387 2455 387 Size: 1791 209 68 3564 Fragments arranged by size: 3564 1791 209 68 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5632 NotI GC'GGCC_GC Cuts at: 3079 3079 Size: 5632 NsiI A_TGCA'T Cuts at: 2907 2911 2907 Size: 4 5628 NspI r_CATG'y Cuts at: 1132 1229 2733 2909 2990 4535 1132 Size: 97 1504 176 81 1545 2229 Fragments arranged by size: 2229 1545 1504 176 97 81 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5632 PciI A'CATG_T Cuts at: 4531 4531 Size: 5632 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5632 PmeI GTTT'AAAC Cuts at: 2936 2936 Size: 5632 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3701 3733 5214 5246 3701 Size: 32 1481 32 4087 Fragments arranged by size: 4087 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5632 PsiI TTA'TAA Cuts at: 2852 2924 3202 2852 Size: 72 278 5282 Fragments arranged by size: 5282 278 72 PstI C_TGCA'G Cuts at: 830 2519 2984 830 Size: 1689 465 3478 Fragments arranged by size: 3478 1689 465 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5632 SalI G'TCGA_C Cuts at: 1936 2974 1936 Size: 1038 4594 SbfI CC_TGCA'GG Cuts at: 2984 2984 Size: 5632 ScaI AGT'ACT Cuts at: 3880 5592 3880 Size: 1712 3920 SfcI C'TryA_G Cuts at: 826 1045 2337 2515 2980 4115 4796 4987 826 Size: 219 1292 178 465 1135 681 191 1471 Fragments arranged by size: 1471 1292 1135 681 465 219 191 178 SfoI GGC'GCC Cuts at: 2737 2737 Size: 5632 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5632 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5632 SmaI CCC'GGG Cuts at: 1872 2959 1872 Size: 1087 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2884 3736 4637 4899 820 Size: 197 926 941 852 901 262 1553 Fragments arranged by size: 1553 941 926 901 852 262 197 SnaBI TAC'GTA Cuts at: 493 3423 493 Size: 2930 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5632 SphI G_CATG'C Cuts at: 1132 2909 2990 1132 Size: 1777 81 3774 Fragments arranged by size: 3774 1777 81 SspI AAT'ATT Cuts at: 5 52 3556 5 Size: 47 3504 2081 Fragments arranged by size: 3504 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5632 StyI C'CwwG_G Cuts at: 513 1065 2004 2072 2124 2137 2197 2246 3071 Size: 552 939 68 52 13 60 49 825 Cuts at: 3071 513 Size: 3074 Fragments arranged by size: 3074 939 825 552 68 60 52 49 13 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2321 3659 3676 3829 4014 4459 325 Size: 1255 741 1338 17 153 185 445 1498 Fragments arranged by size: 1498 1338 1255 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2187 2225 2760 Size: 274 80 33 51 1443 210 38 535 Cuts at: 2760 3878 5590 96 Size: 1118 1712 138 Fragments arranged by size: 1712 1443 1118 535 274 210 138 80 51 38 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5592 5624 5592 Size: 32 5600 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5632 XbaI T'CTAG_A Cuts at: 2968 2968 Size: 5632 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5632 XmaI C'CCGG_G Cuts at: 1870 2957 1870 Size: 1087 4545 XmnI GAAnn'nnTTC Cuts at: 3761 3761 Size: 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3440 276 Size: 53 83 186 936 1906 2468 Fragments arranged by size: 2468 1906 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI