Restriction map for
pFN21AE0577
(Linear) (Six-base) MAPSORT of: KIEE0577.seq Check: 8547 from: 1 to: 927
KIEE0577.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 927
Size: 827 100
AfeI AGC'GCT
Cuts at: 0 429 927
Size: 429 498
AleI CACnn'nnGTG
Cuts at: 0 121 927
Size: 121 806
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 128 160 927
Size: 128 32 767
Fragments arranged by size:
767 128 32
ApoI r'AATT_y
Cuts at: 0 545 563 927
Size: 545 18 364
Fragments arranged by size:
545 364 18
AvrII C'CTAG_G
Cuts at: 0 241 927
Size: 241 686
BanI G'GyrC_C
Cuts at: 0 730 927
Size: 730 197
BbeI G_GCGC'C
Cuts at: 0 734 927
Size: 734 193
BbsI GAAGACnn'nnnn_
Cuts at: 0 815 927
Size: 815 112
BfrBI ATG'CAT
Cuts at: 0 900 904 927
Size: 900 4 23
Fragments arranged by size:
900 23 4
BlpI GC'TnA_GC
Cuts at: 0 801 927
Size: 801 126
BmgBI CAC'GTC
Cuts at: 0 78 927
Size: 78 849
BmrI ACTGGGnnnn_n'
Cuts at: 0 297 927
Size: 297 630
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 927
Size: 900 27
BsaHI Gr'CG_yC
Cuts at: 0 731 927
Size: 731 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 927
Size: 58 869
BsiHKAI G_wGCw'C
Cuts at: 0 671 927
Size: 671 256
BsmI GAATG_Cn'
Cuts at: 0 343 927
Size: 343 584
Bsp1286I G_dGCh'C
Cuts at: 0 671 927
Size: 671 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 927
Size: 503 424
BsrDI GCAATG_nn'
Cuts at: 0 281 579 927
Size: 281 298 348
Fragments arranged by size:
348 298 281
BssSI C'ACGA_G
Cuts at: 0 662 927
Size: 662 265
BtgI C'CryG_G
Cuts at: 0 67 119 927
Size: 67 52 808
Fragments arranged by size:
808 67 52
EarI CTCTTCn'nnn_
Cuts at: 0 88 906 927
Size: 88 818 21
Fragments arranged by size:
818 88 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 927
Size: 827 100
EcoRV GAT'ATC
Cuts at: 0 281 927
Size: 281 646
HaeII r_GCGC'y
Cuts at: 0 431 734 927
Size: 431 303 193
Fragments arranged by size:
431 303 193
KasI G'GCGC_C
Cuts at: 0 730 927
Size: 730 197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 927
Size: 440 487
MslI CAynn'nnrTG
Cuts at: 0 118 121 637 781 927
Size: 118 3 516 144 146
Fragments arranged by size:
516 146 144 118 3
NarI GG'CG_CC
Cuts at: 0 731 927
Size: 731 196
NcoI C'CATG_G
Cuts at: 0 67 119 927
Size: 67 52 808
Fragments arranged by size:
808 67 52
NdeI CA'TA_TG
Cuts at: 0 173 382 450 927
Size: 173 209 68 477
Fragments arranged by size:
477 209 173 68
NsiI A_TGCA'T
Cuts at: 0 902 906 927
Size: 902 4 21
Fragments arranged by size:
902 21 4
NspI r_CATG'y
Cuts at: 0 728 904 927
Size: 728 176 23
Fragments arranged by size:
728 176 23
PsiI TTA'TAA
Cuts at: 0 847 919 927
Size: 847 72 8
Fragments arranged by size:
847 72 8
PstI C_TGCA'G
Cuts at: 0 514 927
Size: 514 413
SfcI C'TryA_G
Cuts at: 0 332 510 927
Size: 332 178 417
Fragments arranged by size:
417 332 178
SfoI GGC'GCC
Cuts at: 0 732 927
Size: 732 195
SmlI C'TyrA_G
Cuts at: 0 879 927
Size: 879 48
SphI G_CATG'C
Cuts at: 0 904 927
Size: 904 23
StyI C'CwwG_G
Cuts at: 0 67 119 132 192 241 927
Size: 67 52 13 60 49 686
Fragments arranged by size:
686 67 60 52 49 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 316 927
Size: 316 611
TatI w'GTAC_w
Cuts at: 0 182 220 755 927
Size: 182 38 535 172
Fragments arranged by size:
535 182 172 38
Enzymes that do cut:
AcuI AfeI AleI AloI ApoI AvrII BanI BbeI
BbsI BfrBI BlpI BmgBI BmrI BpuEI BsaHI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BssSI BtgI EarI
Eco57MI EcoRV HaeII KasI MmeI MslI NarI NcoI
NdeI NsiI NspI PsiI PstI SfcI SfoI SmlI
SphI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BanII BbvCI BcgI BciVI BclI
BglI BglII Bme1580I BmtI BplI BpmI Bpu10I BsaI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
MspA1I NaeI NgoMIV NheI NotI NruI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI