Restriction map for
pFN21AE0575
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# NO CUT ENZYMES ( 155 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII BalI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfrI BglI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HapII HgaI Hin1I HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MseI MspI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI StuI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AflIII 129 ACGTGT A^C[GA][CT]GT
AspHI 71 GTGCTC G[AT]GC[AT]^C
AvrII 120 CCTAGG C^CTAGG
BamHI 515 GGATCC G^GATCC
BanII 105 GAGCCC G[GA]GC[CT]^C
BclI 647 TGATCA T^GATCA
BfaI 121 CTAG C^TAG
BglII 53 AGATCT A^GATCT
BlnI 120 CCTAGG C^CTAGG
BseRI 964 CTCCTC GAGGAG(10/8)
BsiHKAI 71 GTGCTC G[AT]GC[AT]^C
BsmAI 810 GAGAC GTCTC(1/5)
BspMI 1030 ACCTGC ACCTGC(4/8)
BstF5I 503 GGATG GGATG(2/0)
DsaI 542 CCATGG C^C[GA][CT]GG
EaeI 100 TGGCCG [CT]^GGCC[GA]
EarI 724 CTCTTC CTCTTC(1/4)
FbaI 647 TGATCA T^GATCA
FokI 503 GGATG GGATG(9/13)
HincII 717 GTTGAC GT[CT]^[GA]AC
HindII 717 GTTGAC GT[CT]^[GA]AC
Ksp632I 724 CTCTTC CTCTTC(1/4)
MaeI 121 CTAG C^TAG
NcoI 542 CCATGG C^CATGG
PstI 729 CTGCAG CTGCA^G
PvuII 314 CAGCTG CAG^CTG
ScaI 960 AGTACT AGT^ACT
SmlI 1012 CTCAAG C^T[CT][GA]AG
SphI 396 GCATGC GCATG^C
SspI 177 AATATT AAT^ATT
TaqI 527 TCGA T^CGA
TthHB8I 527 TCGA T^CGA
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 287 GAATTT [GA]^AATT[CT]
448 GAATTT
AfaI 756 GTAC GT^AC
961 GTAC
AlwI 516 GATCC GGATC(4/5)
515 GGATC
AlwNI 579 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
860 CAGTCCCTG
ApoI 287 GAATTT [GA]^AATT[CT]
448 GAATTT
BsmI 480 GCATTC GAATGC(1/-1)
823 GCATTC
BstXI 183 CCAAACAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
680 CCACCCAGGTGG
BstYI 53 AGATCT [GA]^GATC[CT]
515 GGATCC
CfoI 376 GCGC GCG^C
420 GCGC
EcoT14I 120 CCTAGG C^C[AT][AT]GG
542 CCATGG
HhaI 376 GCGC GCG^C
420 GCGC
HinP1I 376 GCGC G^CGC
420 GCGC
MaeII 129 ACGT A^CGT
524 ACGT
MflI 53 AGATCT [GA]^GATC[CT]
515 GGATCC
MslI 64 CATGCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
681 CACCCAGGTG
MspA1I 314 CAGCTG C[AC]G^C[GT]G
927 CCGCTG
NdeI 168 CATATG CA^TATG
1035 CATATG
NspI 63 ACATGC [GA]CATG^[CT]
396 GCATGC
PflMI 498 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
859 CCAGTCCCTGG
RsaI 756 GTAC GT^AC
961 GTAC
SfaNI 581 GATGC GCATC(5/9)
881 GATGC
StyI 120 CCTAGG C^C[AT][AT]GG
542 CCATGG
Tsp509I 288 AATT ^AATT
449 AATT
Van91I 498 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
859 CCAGTCCCTGG
XhoII 53 AGATCT [GA]^GATC[CT]
515 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
FauI Fnu4HI HaeIII HinfI HphI
ItaI MaeIII MboI MboII MnlI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI Sau3AI Sau96I ScrFI
SfcI TfiI TseI Tsp45I TspRI
XcmI