(Circular) (Six-base) MAPSORT of: pFN21AE0575.seq Check: 1780 from: 1 to: 5761 pFN21AE0575 5761 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3824 5208 5476 1632 Size: 725 1467 1384 268 1917 Fragments arranged by size: 1917 1467 1384 725 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2134 4660 5637 1203 Size: 931 2526 977 1327 Fragments arranged by size: 2526 1327 977 931 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2690 1166 Size: 1524 4237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2589 2870 3504 5076 2589 Size: 281 634 1572 3274 Fragments arranged by size: 3274 1572 634 281 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 2292 2453 3070 3296 3448 5557 1493 Size: 799 161 617 226 152 2109 1697 Fragments arranged by size: 2109 1697 799 617 226 161 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 AvrII C'CTAG_G Cuts at: 2125 2125 Size: 5761 BamHI G'GATC_C Cuts at: 2520 3091 3458 2520 Size: 571 367 4823 Fragments arranged by size: 4823 571 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3082 4537 618 Size: 325 217 627 1295 1455 1842 Fragments arranged by size: 1842 1455 1295 627 325 217 BanII G_rGCy'C Cuts at: 1391 2114 3080 1391 Size: 723 966 4072 Fragments arranged by size: 4072 966 723 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 2652 1548 Size: 1104 4657 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2058 5756 2058 Size: 3698 2063 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 2478 3822 4978 1790 Size: 688 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 688 BmgBI CAC'GTC Cuts at: 1318 2135 1318 Size: 817 4944 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2205 2625 2918 Size: 416 53 83 399 24 788 420 293 Cuts at: 2918 3492 4450 442 Size: 574 958 1753 Fragments arranged by size: 1753 958 788 574 420 416 399 293 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2347 2987 2347 Size: 640 5121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4421 1239 Size: 3182 2579 Bpu10I CC'TnA_GC Cuts at: 2079 2079 Size: 5761 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3002 3886 4751 5049 3002 Size: 884 865 298 3714 Fragments arranged by size: 3714 884 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4194 4592 4866 5013 5424 4194 Size: 398 274 147 411 4531 Fragments arranged by size: 4531 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2075 2105 2121 2151 2375 Size: 30 165 30 672 30 16 30 224 Cuts at: 2375 2400 2405 2430 1178 Size: 25 5 25 4509 Fragments arranged by size: 4509 672 224 165 30 30 30 30 25 25 16 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2960 1186 Size: 1774 3987 BseYI C'CCAG_C Cuts at: 2490 2516 2710 4964 5460 2490 Size: 26 194 2254 496 2791 Fragments arranged by size: 2791 2254 496 194 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2080 3822 3907 4978 2080 Size: 1742 85 1071 2863 Fragments arranged by size: 2863 1742 1071 85 BsmI GAATG_Cn' Cuts at: 2485 2828 3272 3365 2485 Size: 343 444 93 4881 Fragments arranged by size: 4881 444 343 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2114 2478 3080 3822 3907 4978 Size: 399 290 34 364 602 742 85 1071 Cuts at: 4978 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 602 399 364 290 85 34 BspEI T'CCGG_A Cuts at: 5424 5424 Size: 5761 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3044 3116 844 Size: 2200 72 3489 Fragments arranged by size: 3489 2200 72 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5761 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3821 4833 3821 Size: 1012 4749 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstXI CCAn_nnnn'nTGG Cuts at: 2195 2692 2195 Size: 497 5264 BstYI r'GATC_y Cuts at: 1972 2058 2520 3091 3458 3843 3860 5312 5398 Size: 86 462 571 367 385 17 1452 86 Cuts at: 5398 5410 5756 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 571 462 385 367 346 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2547 513 Size: 552 939 543 3727 Fragments arranged by size: 3727 939 552 543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 3065 3065 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2105 3208 4097 Size: 54 1425 50 275 18 275 1103 889 Cuts at: 4097 5726 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1103 889 275 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 3691 1353 Size: 1382 956 3423 Fragments arranged by size: 3423 1382 956 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4348 4722 4868 387 Size: 1064 138 2759 374 146 1280 Fragments arranged by size: 2759 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 3824 4421 5208 5476 1239 Size: 393 725 1467 597 787 268 1524 Fragments arranged by size: 1524 1467 787 725 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2199 2274 2552 2877 2199 Size: 75 278 325 5083 Fragments arranged by size: 5083 325 278 75 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 4908 1678 Size: 3230 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2075 2107 2784 2816 4402 Size: 32 135 32 503 32 677 32 1586 Cuts at: 4402 4434 4476 4508 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1586 677 503 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2724 3105 3351 669 Size: 988 281 786 381 246 3079 Fragments arranged by size: 3079 988 786 381 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2256 2269 4875 5059 1305 Size: 951 13 2606 184 2007 Fragments arranged by size: 2606 2007 951 184 13 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3939 Fragments arranged by size: 3939 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2690 3719 4078 4237 518 Size: 648 907 617 1029 359 159 2042 Fragments arranged by size: 2042 1029 907 648 617 359 159 MspA1I CmG'CkG Cuts at: 1606 2321 2934 3177 3854 5002 5247 1606 Size: 715 613 243 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 613 245 243 NcoI C'CATG_G Cuts at: 513 1065 2004 2547 513 Size: 552 939 543 3727 Fragments arranged by size: 3727 939 552 543 NdeI CA'TA_TG Cuts at: 387 2174 3041 387 Size: 1787 867 3107 Fragments arranged by size: 3107 1787 867 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2072 2405 3119 4664 1132 Size: 97 843 333 714 1545 2229 Fragments arranged by size: 2229 1545 843 714 333 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 2509 2870 1108 Size: 1401 361 3999 Fragments arranged by size: 3999 1401 361 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2199 2274 2552 2877 2199 Size: 75 278 325 5083 Fragments arranged by size: 5083 325 278 75 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2753 2785 2753 Size: 32 5729 PstI C_TGCA'G Cuts at: 830 2738 3113 830 Size: 1908 375 3478 Fragments arranged by size: 3478 1908 375 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2321 2321 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SanDI GG'GwC_CC Cuts at: 2199 2199 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 2967 4009 5721 2967 Size: 1042 1712 3007 Fragments arranged by size: 3007 1712 1042 SfcI C'TryA_G Cuts at: 826 1045 2278 2581 2734 2881 3109 4244 4925 Size: 219 1233 303 153 147 228 1135 681 Cuts at: 4925 5116 826 Size: 191 1471 Fragments arranged by size: 1471 1233 1135 681 303 228 219 191 153 147 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3017 3865 4766 5028 820 Size: 197 926 1074 848 901 262 1553 Fragments arranged by size: 1553 1074 926 901 848 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 2405 3119 1132 Size: 1273 714 3774 Fragments arranged by size: 3774 1273 714 SspI AAT'ATT Cuts at: 5 52 2184 3685 5 Size: 47 2132 1501 2081 Fragments arranged by size: 2132 2081 1501 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 2125 2547 3200 513 Size: 552 939 121 422 653 3074 Fragments arranged by size: 3074 939 653 552 422 121 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2400 2702 3788 3805 3958 4143 4588 Size: 1255 820 302 1086 17 153 185 445 Cuts at: 4588 325 Size: 1498 Fragments arranged by size: 1498 1255 1086 820 445 302 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2965 4007 5719 Size: 274 80 33 51 1443 988 1042 1712 Cuts at: 5719 96 Size: 138 Fragments arranged by size: 1712 1443 1042 988 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2375 2400 2407 2432 5721 5753 2121 Size: 32 222 25 7 25 3289 32 2129 Fragments arranged by size: 3289 2129 222 32 32 25 25 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2638 2707 2845 2638 Size: 69 138 5554 Fragments arranged by size: 5554 138 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI