(Circular) (Six-base) MAPSORT of: pFN21AE0573.seq Check: 3786 from: 1 to: 5572 pFN21AE0573 5572 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3382 278 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2451 2915 1937 Size: 514 464 4594 Fragments arranged by size: 4594 514 464 Acc65I G'GTAC_C Cuts at: 2893 2893 Size: 5572 AclI AA'CG_TT Cuts at: 1563 3699 4072 1563 Size: 2136 373 3063 Fragments arranged by size: 3063 2136 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2488 3635 5019 5287 1632 Size: 856 1147 1384 268 1917 Fragments arranged by size: 1917 1384 1147 856 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5375 AflIII A'CryG_T Cuts at: 1203 4471 5448 1203 Size: 3268 977 1327 Fragments arranged by size: 3268 1327 977 AgeI A'CCGG_T Cuts at: 4403 4403 Size: 5572 AhdI GACnn_n'nnGTC Cuts at: 4301 4301 Size: 5572 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5572 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5572 AlwNI CAG_nnn'CTG Cuts at: 2078 2263 2377 2511 3315 4887 2078 Size: 185 114 134 804 1572 2763 Fragments arranged by size: 2763 1572 804 185 134 114 ApaLI G'TGCA_C Cuts at: 3629 4785 3629 Size: 1156 4416 ApoI r'AATT_y Cuts at: 1493 2881 3107 3259 5368 1493 Size: 1388 226 152 2109 1697 Fragments arranged by size: 2109 1697 1388 226 152 AseI AT'TA_AT Cuts at: 160 1032 4126 160 Size: 872 3094 1606 Fragments arranged by size: 3094 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 AvaI C'yCGr_G Cuts at: 1870 1943 2017 2417 2897 1870 Size: 73 74 400 480 4545 Fragments arranged by size: 4545 480 400 74 73 BamHI G'GATC_C Cuts at: 2902 3269 2902 Size: 367 5205 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2357 2740 2893 4348 618 Size: 325 217 627 570 383 153 1455 1842 Fragments arranged by size: 1842 1455 627 570 383 325 217 153 BanII G_rGCy'C Cuts at: 1391 2384 2480 2603 2833 2891 1391 Size: 993 96 123 230 58 4072 Fragments arranged by size: 4072 993 230 123 96 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2187 928 Size: 971 288 4313 Fragments arranged by size: 4313 971 288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3763 3797 1903 Size: 34 1826 34 3678 Fragments arranged by size: 3678 1826 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3464 4674 3464 Size: 1210 4362 BclI T'GATC_A Cuts at: 1548 1548 Size: 5572 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2766 4183 136 Size: 107 122 71 631 605 1094 1417 1525 Fragments arranged by size: 1525 1417 1094 631 605 122 107 71 BglII A'GATC_T Cuts at: 5567 5567 Size: 5572 BlpI GC'TnA_GC Cuts at: 2378 2989 2378 Size: 611 4961 Bme1580I G_kGCm'C Cuts at: 1790 2246 2360 2743 3633 4789 1790 Size: 456 114 383 890 1156 2573 Fragments arranged by size: 2573 1156 890 456 383 114 BmgBI CAC'GTC Cuts at: 1318 2863 1318 Size: 1545 4027 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3303 4261 442 Size: 416 53 83 399 24 1886 958 1753 Fragments arranged by size: 1886 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5572 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2043 2043 Size: 5572 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 2240 2285 2307 2513 2716 2747 4232 Size: 803 198 45 22 206 203 31 1485 Cuts at: 4232 1239 Size: 2579 Fragments arranged by size: 2579 1485 803 206 203 198 45 31 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3697 4562 4860 3697 Size: 865 298 4409 Fragments arranged by size: 4409 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2071 2102 2381 4235 882 Size: 617 572 31 279 1854 2219 Fragments arranged by size: 2219 1854 617 572 279 31 BsaAI yAC'GTr Cuts at: 493 1204 2455 3363 493 Size: 711 1251 908 2702 Fragments arranged by size: 2702 1251 908 711 BsaBI GATnn'nnATC Cuts at: 5566 5566 Size: 5572 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2312 2804 2819 3379 Size: 53 83 186 936 779 492 15 560 Cuts at: 3379 3761 4396 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 779 635 560 492 382 186 83 53 15 BsaWI w'CCGG_w Cuts at: 2799 4005 4403 4677 4824 5235 2799 Size: 1206 398 274 147 411 3136 Fragments arranged by size: 3136 1206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2462 2492 2714 2744 1178 Size: 30 165 30 1059 30 222 30 4006 Fragments arranged by size: 4006 1059 222 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2059 2165 2174 2366 2435 2501 1186 Size: 873 106 9 192 69 66 4257 Fragments arranged by size: 4257 873 192 106 69 66 9 BseYI C'CCAG_C Cuts at: 2091 4775 5271 2091 Size: 2684 496 2392 Fragments arranged by size: 2684 2392 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2351 2627 1163 Size: 573 615 276 4108 Fragments arranged by size: 4108 615 573 276 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2367 2837 3022 3783 4811 1540 Size: 275 186 366 470 185 761 1028 2301 Fragments arranged by size: 2301 1028 761 470 366 275 186 185 BsiHKAI G_wGCw'C Cuts at: 3633 3718 4789 3633 Size: 85 1071 4416 Fragments arranged by size: 4416 1071 85 BsmI GAATG_Cn' Cuts at: 3083 3176 3083 Size: 93 5479 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2360 2384 2480 2603 2743 2833 Size: 399 456 114 24 96 123 140 90 Cuts at: 2833 2891 3633 3718 4789 1391 Size: 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 140 123 114 96 90 85 58 24 BspEI T'CCGG_A Cuts at: 5235 5235 Size: 5572 BspHI T'CATG_A Cuts at: 3461 5191 3461 Size: 1730 3842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2927 844 Size: 2083 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2025 3459 1394 Size: 429 202 1434 3507 Fragments arranged by size: 3507 1434 429 202 BsrDI GCAATG_nn' Cuts at: 65 1248 2246 4067 4241 65 Size: 1183 998 1821 174 1396 Fragments arranged by size: 1821 1396 1183 998 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2806 4216 4403 1700 Size: 110 996 1410 187 2869 Fragments arranged by size: 2869 1410 996 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5572 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5572 BssSI C'ACGA_G Cuts at: 3632 4644 3632 Size: 1012 4560 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5572 BstBI TT'CG_AA Cuts at: 4376 4376 Size: 5572 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5572 BstYI r'GATC_y Cuts at: 1972 2444 2902 3269 3654 3671 5123 5209 5221 Size: 472 458 367 385 17 1452 86 12 Cuts at: 5221 5567 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 472 458 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2079 2079 Size: 5572 BtgI C'CryG_G Cuts at: 513 1065 2292 2315 2361 2391 2616 2704 513 Size: 552 1227 23 46 30 225 88 3381 Fragments arranged by size: 3381 1227 552 225 88 46 30 23 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4833 Fragments arranged by size: 4833 683 56 BtsI GCAGTG_nn' Cuts at: 3088 3882 3902 3088 Size: 794 20 4758 Fragments arranged by size: 4758 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5572 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5537 DraI TTT'AAA Cuts at: 2876 2876 Size: 5572 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4579 809 Size: 507 3263 1802 Fragments arranged by size: 3263 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2364 2834 3019 Size: 54 1425 50 275 18 534 470 185 Cuts at: 3019 3908 5537 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 534 470 275 185 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2364 2834 3019 1537 Size: 275 552 470 185 4090 Fragments arranged by size: 4090 552 470 275 185 EarI CTCTTCn'nnn_ Cuts at: 1353 2036 2160 2343 2642 3502 1353 Size: 683 124 183 299 860 3423 Fragments arranged by size: 3423 860 683 299 183 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2470 4159 4533 4679 387 Size: 1064 138 881 1689 374 146 1280 Fragments arranged by size: 1689 1280 1064 881 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 2240 2285 2307 2488 2513 2716 Size: 393 410 198 45 22 181 25 203 Cuts at: 2716 2747 3635 4232 5019 5287 1239 Size: 31 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 597 410 393 268 203 198 181 45 31 25 22 EcoO109I rG'GnC_Cy Cuts at: 2097 2412 2710 2772 2097 Size: 315 298 62 4897 Fragments arranged by size: 4897 315 298 62 EcoRI G'AATT_C Cuts at: 2881 3259 2881 Size: 378 5194 FspI TGC'GCA Cuts at: 4078 4078 Size: 5572 HaeII r_GCGC'y Cuts at: 1678 4719 1678 Size: 3041 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2429 2461 2520 2537 2552 Size: 32 135 32 857 32 59 17 15 Cuts at: 2552 2569 4213 4245 4287 4319 1373 Size: 17 1644 32 42 32 2626 Fragments arranged by size: 2626 1644 857 135 59 42 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2916 3162 669 Size: 988 281 978 246 3079 Fragments arranged by size: 3079 988 978 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5572 HpaI GTT'AAC Cuts at: 3162 3162 Size: 5572 KpnI G_GTAC'C Cuts at: 2897 2897 Size: 5572 MfeI C'AATT_G Cuts at: 3171 3171 Size: 5572 MluI A'CGCG_T Cuts at: 5448 5448 Size: 5572 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4686 4870 1305 Size: 3381 184 2007 Fragments arranged by size: 3381 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3750 Fragments arranged by size: 3750 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 2657 3530 3889 4048 518 Size: 648 1110 381 873 359 159 2042 Fragments arranged by size: 2042 1110 873 648 381 359 159 MspA1I CmG'CkG Cuts at: 1606 2363 2374 2511 2706 2757 2839 2988 3665 Size: 757 11 137 195 51 82 149 677 Cuts at: 3665 4813 5058 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 757 677 245 195 149 137 82 51 11 NaeI GCC'GGC Cuts at: 2808 2808 Size: 5572 NcoI C'CATG_G Cuts at: 513 1065 2292 513 Size: 552 1227 3793 Fragments arranged by size: 3793 1227 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5572 NgoMIV G'CCGG_C Cuts at: 2806 2806 Size: 5572 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5572 NotI GC'GGCC_GC Cuts at: 3019 3019 Size: 5572 NspI r_CATG'y Cuts at: 1132 1229 2930 4475 1132 Size: 97 1701 1545 2229 Fragments arranged by size: 2229 1701 1545 97 PasI CC'CwG_GG Cuts at: 1348 2086 2684 2844 1348 Size: 738 598 160 4076 Fragments arranged by size: 4076 738 598 160 PciI A'CATG_T Cuts at: 4471 4471 Size: 5572 PflMI CCAn_nnn'nTGG Cuts at: 1108 2135 2263 1108 Size: 1027 128 4417 Fragments arranged by size: 4417 1027 128 PfoI T'CCnGG_A Cuts at: 2323 2323 Size: 5572 PmeI GTTT'AAAC Cuts at: 2876 2876 Size: 5572 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3641 3673 5154 5186 3641 Size: 32 1481 32 4027 Fragments arranged by size: 4027 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2412 2412 Size: 5572 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5572 PsiI TTA'TAA Cuts at: 3142 3142 Size: 5572 PstI C_TGCA'G Cuts at: 830 2924 830 Size: 2094 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5572 PvuII CAG'CTG Cuts at: 2374 2374 Size: 5572 SacII CC_GC'GG Cuts at: 2364 2707 2364 Size: 343 5229 SalI G'TCGA_C Cuts at: 1936 2914 1936 Size: 978 4594 SanDI GG'GwC_CC Cuts at: 2412 2412 Size: 5572 SbfI CC_TGCA'GG Cuts at: 2924 2924 Size: 5572 ScaI AGT'ACT Cuts at: 3820 5532 3820 Size: 1712 3860 SfcI C'TryA_G Cuts at: 826 1045 2920 4055 4736 4927 826 Size: 219 1875 1135 681 191 1471 Fragments arranged by size: 1875 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5572 SgrAI Cr'CCGG_yG Cuts at: 1700 2806 1700 Size: 1106 4466 SmaI CCC'GGG Cuts at: 1872 2899 1872 Size: 1027 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3676 4577 4839 820 Size: 197 926 1733 901 262 1553 Fragments arranged by size: 1733 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3363 493 Size: 2870 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5572 SphI G_CATG'C Cuts at: 1132 2930 1132 Size: 1798 3774 SspI AAT'ATT Cuts at: 5 52 3496 5 Size: 47 3444 2081 Fragments arranged by size: 3444 2081 47 StuI AGG'CCT Cuts at: 1517 2051 1517 Size: 534 5038 StyI C'CwwG_G Cuts at: 513 1065 2112 2292 2565 2595 3011 513 Size: 552 1047 180 273 30 416 3074 Fragments arranged by size: 3074 1047 552 416 273 180 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2739 2836 3599 3616 3769 3954 4399 Size: 1255 1159 97 763 17 153 185 445 Cuts at: 4399 325 Size: 1498 Fragments arranged by size: 1498 1255 1159 763 445 185 153 97 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3818 5530 96 Size: 274 80 33 51 1443 1841 1712 138 Fragments arranged by size: 1841 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 5532 5564 2714 Size: 32 2786 32 2722 Fragments arranged by size: 2786 2722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5572 XbaI T'CTAG_A Cuts at: 2908 2908 Size: 5572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2119 2119 Size: 5572 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5572 XmaI C'CCGG_G Cuts at: 1870 2897 1870 Size: 1027 4545 XmnI GAAnn'nnTTC Cuts at: 3701 3701 Size: 5572 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3380 276 Size: 53 83 186 936 1846 2468 Fragments arranged by size: 2468 1846 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI