Restriction map for
pFN21AE0572
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstUI
BstYI BstZ17I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AciI 306 CCGC CCGC(-3/-1)
AcyI 49 GACGCC G[GA]^CG[CT]C
AfaI 701 GTAC GT^AC
AocI 422 CCTCAGG CC^T[ACGT]AGG
ApaI 567 GGGCCC GGGCC^C
AspHI 286 GAGCAC G[AT]GC[AT]^C
AvaI 85 CTCGGG C^[CT]CG[GA]G
BanI 3 GGCACC G^G[CT][GA]CC
BbsI 691 GTCTTC GAAGAC(2/6)
BclI 131 TGATCA T^GATCA
BcnI 138 CCGGG CC^[GC]GG
BfaI 698 CTAG C^TAG
BglI 323 GCCCTGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 115 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 115 GCTGAGC GC^T[ACGT]AGC
BpuAI 691 GTCTTC GAAGAC(2/6)
BsaHI 49 GACGCC G[GA]^CG[CT]C
BseRI 44 GAGGAG GAGGAG(10/8)
BsgI 23 CTGCAC GTGCAG(16/14)
BsiHKAI 286 GAGCAC G[AT]GC[AT]^C
BsmBI 47 GAGACG CGTCTC(1/5)
BsoBI 85 CTCGGG C^[CT]CG[GA]G
BspMI 630 GCAGGT ACCTGC(4/8)
BstEII 417 GGTGACC G^GT[ACGT]ACC
BstPI 417 GGTGACC G^GT[ACGT]ACC
Bsu36I 422 CCTCAGG CC^T[ACGT]AGG
CelII 115 GCTGAGC GC^T[ACGT]AGC
DpnII 132 GATC ^GATC
DsaI 7 CCATGG C^C[GA][CT]GG
Eco81I 422 CCTCAGG CC^T[ACGT]AGG
EcoO65I 417 GGTGACC G^GT[ACGT]ACC
FbaI 131 TGATCA T^GATCA
HgaI 49 GACGC GACGC(5/10)
Hin1I 49 GACGCC G[GA]^CG[CT]C
MaeI 698 CTAG C^TAG
MaeII 434 ACGT A^CGT
MboI 132 GATC ^GATC
MboII 692 TCTTC GAAGA(8/7)
MseI 200 TTAA T^TAA
NciI 138 CCGGG CC^[GC]GG
NcoI 7 CCATGG C^CATGG
NdeII 132 GATC ^GATC
PflMI 104 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 520 GACTC GAGTC(4/5)
PpuMI 169 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 701 GTAC GT^AC
Sau3AI 132 GATC ^GATC
TaqI 467 TCGA T^CGA
Tru9I 200 TTAA T^TAA
Tsp509I 705 AATT ^AATT
TthHB8I 467 TCGA T^CGA
Van91I 104 CCACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 170 GGACC G^G[AT]CC
512 GGACC
BsaWI 158 TCCGGT [AT]^CCGG[AT]
666 TCCGGT
BsmFI 169 GGGAC GGGAC(10/14)
224 GGGAC
BstAPI 621 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
749 GCAGCCCCTGC
BstXI 214 CCAGAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
407 CCAGTCCTGTGG
Cac8I 328 GCAGGC GC[ACGT]^[ACGT]GC
617 GCAGGC
EcoT14I 7 CCATGG C^C[AT][AT]GG
515 CCTTGG
PvuII 28 CAGCTG CAG^CTG
526 CAGCTG
SbfI 325 CCTGCAGG CCTGCA^GG
627 CCTGCAGG
SfaNI 594 GATGC GCATC(5/9)
602 GATGC
Sse8387I 325 CCTGCAGG CCTGCA^GG
627 CCTGCAGG
StyI 7 CCATGG C^C[AT][AT]GG
515 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AluI AlwNI BanII BbvI BmyI
BpmI BsaI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeIII
MnlI MspA1I MspI MvaI MwoI
NlaIII NlaIV PstI Sau96I ScrFI
SfcI TfiI TseI Tsp45I TspRI
XcmI