Restriction map for
pFN21AE0572
(Circular) (Six-base) MAPSORT of: pFN21AE0572.seq Check: 1379 from: 1 to: 5464
pFN21AE0572 5464 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3274 278
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2807 1937
Size: 870 4594
Acc65I G'GTAC_C
Cuts at: 2785 2785
Size: 5464
AclI AA'CG_TT
Cuts at: 1563 3591 3964 1563
Size: 2028 373 3063
Fragments arranged by size:
3063 2028 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3527 4911 5179 1632
Size: 1895 1384 268 1917
Fragments arranged by size:
1917 1895 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5267
AflIII A'CryG_T
Cuts at: 1203 4363 5340 1203
Size: 3160 977 1327
Fragments arranged by size:
3160 1327 977
AgeI A'CCGG_T
Cuts at: 4295 4295
Size: 5464
AhdI GACnn_n'nnGTC
Cuts at: 4193 4193
Size: 5464
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5464
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5464
AlwNI CAG_nnn'CTG
Cuts at: 2132 2560 2760 3207 4779 2132
Size: 428 200 447 1572 2817
Fragments arranged by size:
2817 1572 447 428 200
ApaI G_GGCC'C
Cuts at: 2576 2576
Size: 5464
ApaLI G'TGCA_C
Cuts at: 3521 4677 3521
Size: 1156 4308
ApoI r'AATT_y
Cuts at: 1493 2773 2999 3151 5260 1493
Size: 1280 226 152 2109 1697
Fragments arranged by size:
2109 1697 1280 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4018 160
Size: 872 2986 1606
Fragments arranged by size:
2986 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
AvaI C'yCGr_G
Cuts at: 1870 1943 2090 2789 1870
Size: 73 147 699 4545
Fragments arranged by size:
4545 699 147 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2299 2332 2299
Size: 33 5431
BamHI G'GATC_C
Cuts at: 2794 3161 2794
Size: 367 5097
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2785 4240 618
Size: 325 217 627 221 777 1455 1842
Fragments arranged by size:
1842 1455 777 627 325 221 217
BanII G_rGCy'C
Cuts at: 1391 2066 2253 2349 2576 2783 1391
Size: 675 187 96 227 207 4072
Fragments arranged by size:
4072 675 227 207 187 96
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2689 928
Size: 971 790 3703
Fragments arranged by size:
3703 971 790
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3655 3689 1903
Size: 34 1718 34 3678
Fragments arranged by size:
3678 1718 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3356 4566 3356
Size: 1210 4254
BclI T'GATC_A
Cuts at: 1548 2136 1548
Size: 588 4876
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2334 4075 136
Size: 107 122 71 631 605 662 1741 1525
Fragments arranged by size:
1741 1525 662 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5459 5459
Size: 5464
BlpI GC'TnA_GC
Cuts at: 2121 2881 2121
Size: 760 4704
Bme1580I G_kGCm'C
Cuts at: 1790 2576 3525 4681 1790
Size: 786 949 1156 2573
Fragments arranged by size:
2573 1156 949 786
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5464
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2406 2535 2646
Size: 416 53 83 399 24 989 129 111
Cuts at: 2646 3195 4153 442
Size: 549 958 1753
Fragments arranged by size:
1753 989 958 549 416 399 129 111
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5464
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 5464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2267 2381 2432 2684 4124 1239
Size: 962 66 114 51 252 1440 2579
Fragments arranged by size:
2579 1440 962 252 114 66 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3589 4454 4752 3589
Size: 865 298 4301
Fragments arranged by size:
4301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2022 2493 2680 4127 882
Size: 617 523 471 187 1447 2219
Fragments arranged by size:
2219 1447 617 523 471 187
BsaAI yAC'GTr
Cuts at: 493 1204 3255 493
Size: 711 2051 2702
Fragments arranged by size:
2702 2051 711
BsaBI GATnn'nnATC
Cuts at: 5458 5458
Size: 5464
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2055 3271 3653 4288
Size: 53 83 186 936 522 1216 382 635
Cuts at: 4288 275
Size: 1451
Fragments arranged by size:
1451 1216 936 635 522 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2163 2671 3897 4295 4569 4716 5127 2163
Size: 508 1226 398 274 147 411 2500
Fragments arranged by size:
2500 1226 508 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2043 2073 2424 2454 2495
Size: 30 165 30 640 30 351 30 41
Cuts at: 2495 2525 1178
Size: 30 4117
Fragments arranged by size:
4117 640 351 165 41 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2064 1186
Size: 878 4586
BseYI C'CCAG_C
Cuts at: 2348 2585 4667 5163 2348
Size: 237 2082 496 2649
Fragments arranged by size:
2649 2082 496 237
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2013 1163
Size: 573 277 4614
Fragments arranged by size:
4614 573 277
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2914 3675 4703 1540
Size: 275 186 913 761 1028 2301
Fragments arranged by size:
2301 1028 913 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 3525 3610 4681 2295
Size: 1230 85 1071 3078
Fragments arranged by size:
3078 1230 1071 85
BsmI GAATG_Cn'
Cuts at: 2975 3068 2975
Size: 93 5371
BsmBI CGTCTCn'nnnn_
Cuts at: 2046 2046
Size: 5464
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2066 2253 2295 2349 2576 2783 3525
Size: 399 276 187 42 54 227 207 742
Cuts at: 3525 3610 4681 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 276 227 207 187
85 54 42
BspEI T'CCGG_A
Cuts at: 5127 5127
Size: 5464
BspHI T'CATG_A
Cuts at: 3353 5083 3353
Size: 1730 3734
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2626 2819 844
Size: 1782 193 3489
Fragments arranged by size:
3489 1782 193
BsrBI CCG'CTC
Cuts at: 1394 1823 3351 1394
Size: 429 1528 3507
Fragments arranged by size:
3507 1528 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3959 4133 65
Size: 1183 2711 174 1396
Fragments arranged by size:
2711 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4108 4295 1700
Size: 110 2298 187 2869
Fragments arranged by size:
2869 2298 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5464
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5464
BssSI C'ACGA_G
Cuts at: 3524 4536 3524
Size: 1012 4452
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2632 2760 794
Size: 1838 128 3498
Fragments arranged by size:
3498 1838 128
BstBI TT'CG_AA
Cuts at: 4268 4268
Size: 5464
BstEII G'GTnAC_C
Cuts at: 1184 2422 1184
Size: 1238 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 2226 2419 2226
Size: 193 5271
BstYI r'GATC_y
Cuts at: 1972 2794 3161 3546 3563 5015 5101 5113 5459
Size: 822 367 385 17 1452 86 12 346
Cuts at: 5459 1972
Size: 1977
Fragments arranged by size:
1977 1452 822 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2428 2428
Size: 5464
BtgI C'CryG_G
Cuts at: 513 1065 2004 2012 513
Size: 552 939 8 3965
Fragments arranged by size:
3965 939 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4725
Fragments arranged by size:
4725 683 56
BtsI GCAGTG_nn'
Cuts at: 2980 3774 3794 2980
Size: 794 20 4650
Fragments arranged by size:
4650 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5464
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5429
DraI TTT'AAA
Cuts at: 2768 2768
Size: 5464
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4471 809
Size: 507 3155 1802
Fragments arranged by size:
3155 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2911 3800 5429
Size: 54 1425 50 275 18 1081 889 1629
Cuts at: 5429 8
Size: 43
Fragments arranged by size:
1629 1425 1081 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2911 1537
Size: 275 1099 4090
Fragments arranged by size:
4090 1099 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3394 1353
Size: 2041 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4051 4425 4571 387
Size: 1064 138 2462 374 146 1280
Fragments arranged by size:
2462 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2267 2381 2432 2684 3527 4124
Size: 393 569 66 114 51 252 843 597
Cuts at: 4124 4911 5179 1239
Size: 787 268 1524
Fragments arranged by size:
1524 843 787 597 569 393 268 252
114 66 51
EcoO109I rG'GnC_Cy
Cuts at: 2175 2557 2573 2175
Size: 382 16 5066
Fragments arranged by size:
5066 382 16
EcoRI G'AATT_C
Cuts at: 2773 3151 2773
Size: 378 5086
FspI TGC'GCA
Cuts at: 3970 3970
Size: 5464
HaeII r_GCGC'y
Cuts at: 1678 4611 1678
Size: 2933 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2259 2291 4105 4137 4179
Size: 32 135 32 687 32 1814 32 42
Cuts at: 4179 4211 1373
Size: 32 2626
Fragments arranged by size:
2626 1814 687 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2808 3054 669
Size: 988 281 870 246 3079
Fragments arranged by size:
3079 988 870 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5464
HpaI GTT'AAC
Cuts at: 3054 3054
Size: 5464
KpnI G_GTAC'C
Cuts at: 2789 2789
Size: 5464
MfeI C'AATT_G
Cuts at: 3063 3063
Size: 5464
MluI A'CGCG_T
Cuts at: 5340 5340
Size: 5464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4578 4762 1305
Size: 3273 184 2007
Fragments arranged by size:
3273 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3642
Fragments arranged by size:
3642 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3422 3781 3940 518
Size: 648 2256 359 159 2042
Fragments arranged by size:
2256 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2313 2533 2880 3557 4705 4950 1606
Size: 429 278 220 347 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 429 347 278 245 220
NcoI C'CATG_G
Cuts at: 513 1065 2004 2012 513
Size: 552 939 8 3965
Fragments arranged by size:
3965 939 552 8
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5464
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5464
NotI GC'GGCC_GC
Cuts at: 2911 2911
Size: 5464
NspI r_CATG'y
Cuts at: 1132 1229 2822 4367 1132
Size: 97 1593 1545 2229
Fragments arranged by size:
2229 1593 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2226 1348
Size: 878 4586
PciI A'CATG_T
Cuts at: 4363 4363
Size: 5464
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2115 1108
Size: 1007 4457
PfoI T'CCnGG_A
Cuts at: 2513 2661 2513
Size: 148 5316
PmeI GTTT'AAAC
Cuts at: 2768 2768
Size: 5464
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 5046 5078 3533
Size: 32 1481 32 3919
Fragments arranged by size:
3919 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2175 2175
Size: 5464
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5464
PsiI TTA'TAA
Cuts at: 3034 3034
Size: 5464
PspOMI G'GGCC_C
Cuts at: 2572 2572
Size: 5464
PstI C_TGCA'G
Cuts at: 830 2040 2335 2637 2816 830
Size: 1210 295 302 179 3478
Fragments arranged by size:
3478 1210 302 295 179
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5464
PvuII CAG'CTG
Cuts at: 2035 2533 2035
Size: 498 4966
SalI G'TCGA_C
Cuts at: 1936 2806 1936
Size: 870 4594
SanDI GG'GwC_CC
Cuts at: 2175 2175
Size: 5464
SbfI CC_TGCA'GG
Cuts at: 2335 2637 2816 2335
Size: 302 179 4983
Fragments arranged by size:
4983 302 179
ScaI AGT'ACT
Cuts at: 3712 5424 3712
Size: 1712 3752
SfcI C'TryA_G
Cuts at: 826 1045 2036 2323 2331 2633 2812 3947 4628
Size: 219 991 287 8 302 179 1135 681
Cuts at: 4628 4819 826
Size: 191 1471
Fragments arranged by size:
1471 1135 991 681 302 287 219 191
179 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5464
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5464
SmaI CCC'GGG
Cuts at: 1872 2791 1872
Size: 919 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3568 4469 4731 820
Size: 197 926 1625 901 262 1553
Fragments arranged by size:
1625 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3255 493
Size: 2762 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5464
SphI G_CATG'C
Cuts at: 1132 2822 1132
Size: 1690 3774
SspI AAT'ATT
Cuts at: 5 52 3388 5
Size: 47 3336 2081
Fragments arranged by size:
3336 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5464
StyI C'CwwG_G
Cuts at: 513 1065 2004 2012 2520 2903 513
Size: 552 939 8 508 383 3074
Fragments arranged by size:
3074 939 552 508 383 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3491 3508 3661 3846 4291 325
Size: 1255 1911 17 153 185 445 1498
Fragments arranged by size:
1911 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2705 3710 5422
Size: 274 80 33 51 1443 728 1005 1712
Cuts at: 5422 96
Size: 138
Fragments arranged by size:
1712 1443 1005 728 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2043 2075 2424 2456 2495 2527 5424 5456 2043
Size: 32 349 32 39 32 2897 32 2051
Fragments arranged by size:
2897 2051 349 39 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5464
XbaI T'CTAG_A
Cuts at: 2800 2800
Size: 5464
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2356 2659 2729 2356
Size: 303 70 5091
Fragments arranged by size:
5091 303 70
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5464
XmaI C'CCGG_G
Cuts at: 1870 2789 1870
Size: 919 4545
XmnI GAAnn'nnTTC
Cuts at: 3593 3593
Size: 5464
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3272 276
Size: 53 83 186 936 1738 2468
Fragments arranged by size:
2468 1738 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspXI PsrI RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI