(Circular) (Six-base) MAPSORT of: pFN21AE0571.seq Check: 6966 from: 1 to: 5269 pFN21AE0571 5269 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2362 2362 Size: 5269 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3079 278 Size: 53 83 186 936 1543 2468 Fragments arranged by size: 2468 1543 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2612 1937 Size: 675 4594 Acc65I G'GTAC_C Cuts at: 2590 2590 Size: 5269 AclI AA'CG_TT Cuts at: 1563 3396 3769 1563 Size: 1833 373 3063 Fragments arranged by size: 3063 1833 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 3332 4716 4984 1632 Size: 680 1020 1384 268 1917 Fragments arranged by size: 1917 1384 1020 680 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5072 AflIII A'CryG_T Cuts at: 1203 4168 5145 1203 Size: 2965 977 1327 Fragments arranged by size: 2965 1327 977 AgeI A'CCGG_T Cuts at: 4100 4100 Size: 5269 AhdI GACnn_n'nnGTC Cuts at: 3998 3998 Size: 5269 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5269 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5269 AlwNI CAG_nnn'CTG Cuts at: 3012 4584 3012 Size: 1572 3697 ApaLI G'TGCA_C Cuts at: 3326 4482 3326 Size: 1156 4113 ApoI r'AATT_y Cuts at: 1493 2578 2804 2956 5065 1493 Size: 1085 226 152 2109 1697 Fragments arranged by size: 2109 1697 1085 226 152 AseI AT'TA_AT Cuts at: 160 1032 3823 160 Size: 872 2791 1606 Fragments arranged by size: 2791 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5269 AvaI C'yCGr_G Cuts at: 1870 1943 2594 1870 Size: 73 651 4545 Fragments arranged by size: 4545 651 73 BamHI G'GATC_C Cuts at: 2599 2966 2599 Size: 367 4902 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2590 4045 618 Size: 325 217 627 319 484 1455 1842 Fragments arranged by size: 1842 1455 627 484 325 319 217 BanII G_rGCy'C Cuts at: 1391 2089 2588 1391 Size: 698 499 4072 Fragments arranged by size: 4072 698 499 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2470 928 Size: 971 571 3727 Fragments arranged by size: 3727 971 571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3460 3494 1903 Size: 34 1523 34 3678 Fragments arranged by size: 3678 1523 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3161 4371 3161 Size: 1210 4059 BclI T'GATC_A Cuts at: 1548 1548 Size: 5269 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2519 3880 136 Size: 107 122 71 631 605 847 1361 1525 Fragments arranged by size: 1525 1361 847 631 605 122 107 71 BglII A'GATC_T Cuts at: 5264 5264 Size: 5269 BlpI GC'TnA_GC Cuts at: 2686 2686 Size: 5269 Bme1580I G_kGCm'C Cuts at: 1790 2524 3330 4486 1790 Size: 734 806 1156 2573 Fragments arranged by size: 2573 1156 806 734 BmgBI CAC'GTC Cuts at: 1318 2260 1318 Size: 942 4327 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3000 3958 442 Size: 416 53 83 399 24 1583 958 1753 Fragments arranged by size: 1753 1583 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5269 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2324 3929 1239 Size: 861 224 1605 2579 Fragments arranged by size: 2579 1605 861 224 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 2539 3394 4259 4557 2150 Size: 389 855 865 298 2862 Fragments arranged by size: 2862 865 855 389 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3932 882 Size: 617 2433 2219 Fragments arranged by size: 2433 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3060 493 Size: 711 1856 2702 Fragments arranged by size: 2702 1856 711 BsaBI GATnn'nnATC Cuts at: 5263 5263 Size: 5269 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3076 3458 4093 275 Size: 53 83 186 936 1543 382 635 1451 Fragments arranged by size: 1543 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3702 4100 4374 4521 4932 3702 Size: 398 274 147 411 4039 Fragments arranged by size: 4039 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5044 Fragments arranged by size: 5044 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 1186 Size: 911 4358 BseYI C'CCAG_C Cuts at: 4472 4968 4472 Size: 496 4773 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4696 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2719 3480 4508 1540 Size: 275 186 718 761 1028 2301 Fragments arranged by size: 2301 1028 761 718 275 186 BsiHKAI G_wGCw'C Cuts at: 2035 3330 3415 4486 2035 Size: 1295 85 1071 2818 Fragments arranged by size: 2818 1295 1071 85 BsmI GAATG_Cn' Cuts at: 2780 2873 2780 Size: 93 5176 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2089 2524 2588 3330 3415 4486 Size: 399 245 54 435 64 742 85 1071 Cuts at: 4486 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 435 399 245 85 64 54 BspEI T'CCGG_A Cuts at: 4932 4932 Size: 5269 BspHI T'CATG_A Cuts at: 3158 4888 3158 Size: 1730 3539 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2000 2362 2624 844 Size: 1156 362 262 3489 Fragments arranged by size: 3489 1156 362 262 BsrBI CCG'CTC Cuts at: 1394 1823 3156 1394 Size: 429 1333 3507 Fragments arranged by size: 3507 1333 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2400 3764 3938 65 Size: 1183 1152 1364 174 1396 Fragments arranged by size: 1396 1364 1183 1152 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3913 4100 1700 Size: 110 2103 187 2869 Fragments arranged by size: 2869 2103 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5269 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5269 BssSI C'ACGA_G Cuts at: 2393 3329 4341 2393 Size: 936 1012 3321 Fragments arranged by size: 3321 1012 936 BstAPI GCAn_nnn'nTGC Cuts at: 794 2113 794 Size: 1319 3950 BstBI TT'CG_AA Cuts at: 4073 4073 Size: 5269 BstEII G'GTnAC_C Cuts at: 1184 2341 1184 Size: 1157 4112 BstXI CCAn_nnnn'nTGG Cuts at: 2473 2473 Size: 5269 BstYI r'GATC_y Cuts at: 1972 2599 2966 3351 3368 4820 4906 4918 5264 Size: 627 367 385 17 1452 86 12 346 Cuts at: 5264 1972 Size: 1977 Fragments arranged by size: 1977 1452 627 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3778 Fragments arranged by size: 3778 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4530 Fragments arranged by size: 4530 683 56 BtsI GCAGTG_nn' Cuts at: 2441 2785 3579 3599 2441 Size: 344 794 20 4111 Fragments arranged by size: 4111 794 344 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5234 DraI TTT'AAA Cuts at: 2573 2573 Size: 5269 DraIII CAC_nnn'GTG Cuts at: 2113 2113 Size: 5269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4276 809 Size: 507 2960 1802 Fragments arranged by size: 2960 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2716 3605 5234 Size: 54 1425 50 275 18 886 889 1629 Cuts at: 5234 8 Size: 43 Fragments arranged by size: 1629 1425 889 886 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2716 1537 Size: 275 904 4090 Fragments arranged by size: 4090 904 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 3199 1353 Size: 875 971 3423 Fragments arranged by size: 3423 971 875 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2087 2258 3856 4230 4376 387 Size: 1064 138 498 171 1598 374 146 1280 Fragments arranged by size: 1598 1280 1064 498 374 171 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2312 2324 3332 3929 4716 4984 Size: 393 468 212 12 1008 597 787 268 Cuts at: 4984 1239 Size: 1524 Fragments arranged by size: 1524 1008 787 597 468 393 268 212 12 EcoNI CCTnn'n_nnAGG Cuts at: 2420 2420 Size: 5269 EcoO109I rG'GnC_Cy Cuts at: 2550 2550 Size: 5269 EcoRI G'AATT_C Cuts at: 2578 2956 2578 Size: 378 4891 FspI TGC'GCA Cuts at: 3775 3775 Size: 5269 HaeII r_GCGC'y Cuts at: 1678 4416 1678 Size: 2738 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2148 2164 2180 3910 Size: 32 135 32 560 16 16 16 1730 Cuts at: 3910 3942 3984 4016 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1730 560 135 42 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 2613 2859 669 Size: 988 281 675 246 3079 Fragments arranged by size: 3079 988 675 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5269 HpaI GTT'AAC Cuts at: 2859 2859 Size: 5269 KpnI G_GTAC'C Cuts at: 2594 2594 Size: 5269 MfeI C'AATT_G Cuts at: 2868 2868 Size: 5269 MluI A'CGCG_T Cuts at: 5145 5145 Size: 5269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 2301 4383 4567 1305 Size: 906 90 2082 184 2007 Fragments arranged by size: 2082 2007 906 184 90 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3447 Fragments arranged by size: 3447 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3227 3586 3745 518 Size: 648 2061 359 159 2042 Fragments arranged by size: 2061 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2685 3362 4510 4755 1606 Size: 420 659 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 659 420 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3778 Fragments arranged by size: 3778 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5269 NotI GC'GGCC_GC Cuts at: 2716 2716 Size: 5269 NspI r_CATG'y Cuts at: 1132 1229 2254 2627 4172 1132 Size: 97 1025 373 1545 2229 Fragments arranged by size: 2229 1545 1025 373 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5269 PciI A'CATG_T Cuts at: 4168 4168 Size: 5269 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5269 PmeI GTTT'AAAC Cuts at: 2573 2573 Size: 5269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3338 3370 4851 4883 3338 Size: 32 1481 32 3724 Fragments arranged by size: 3724 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5269 PsiI TTA'TAA Cuts at: 2839 2839 Size: 5269 PstI C_TGCA'G Cuts at: 830 2373 2621 830 Size: 1543 248 3478 Fragments arranged by size: 3478 1543 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5269 PvuII CAG'CTG Cuts at: 2026 2026 Size: 5269 SalI G'TCGA_C Cuts at: 1936 2611 1936 Size: 675 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2228 Size: 5269 SbfI CC_TGCA'GG Cuts at: 2373 2621 2373 Size: 248 5021 ScaI AGT'ACT Cuts at: 3517 5229 3517 Size: 1712 3557 SfcI C'TryA_G Cuts at: 826 1045 2369 2617 3752 4433 4624 826 Size: 219 1324 248 1135 681 191 1471 Fragments arranged by size: 1471 1324 1135 681 248 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5269 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5269 SmaI CCC'GGG Cuts at: 1872 2596 1872 Size: 724 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 2554 3373 4274 4536 820 Size: 197 926 186 425 819 901 262 1553 Fragments arranged by size: 1553 926 901 819 425 262 197 186 SnaBI TAC'GTA Cuts at: 493 3060 493 Size: 2567 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5269 SphI G_CATG'C Cuts at: 1132 2254 2627 1132 Size: 1122 373 3774 Fragments arranged by size: 3774 1122 373 SspI AAT'ATT Cuts at: 5 52 3193 5 Size: 47 3141 2081 Fragments arranged by size: 3141 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5269 StyI C'CwwG_G Cuts at: 513 1065 2004 2708 513 Size: 552 939 704 3074 Fragments arranged by size: 3074 939 704 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3296 3313 3466 3651 4096 325 Size: 1255 1716 17 153 185 445 1498 Fragments arranged by size: 1716 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3515 5227 96 Size: 274 80 33 51 1443 1538 1712 138 Fragments arranged by size: 1712 1538 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5229 5261 5229 Size: 32 5237 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5269 XbaI T'CTAG_A Cuts at: 2605 2605 Size: 5269 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2236 2236 Size: 5269 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5269 XmaI C'CCGG_G Cuts at: 1870 2594 1870 Size: 724 4545 XmnI GAAnn'nnTTC Cuts at: 3398 3398 Size: 5269 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3077 276 Size: 53 83 186 936 1543 2468 Fragments arranged by size: 2468 1543 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI