################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT14I EcoT22I FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MaeII MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AciI 560 CCGC CCGC(-3/-1) AfaI 952 GTAC GT^AC AlwI 366 GGATC GGATC(4/5) ApaI 817 GGGCCC GGGCC^C AvaI 1011 CTCGGG C^[CT]CG[GA]G AvaII 1032 GGTCC G^G[AT]CC BbvCI 397 GCTGAGG CCTCAGC(-5/-2) BglI 649 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 345 CTCCAG CTGGAG(16/14) BsaBI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 1010 CCTCGG C^C[ACGT][ACGT]GG BsmI 160 GAATGC GAATGC(1/-1) BsoBI 1011 CTCGGG C^[CT]CG[GA]G BspHI 876 TCATGA T^CATGA BstAPI 988 GCATGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 75 GGTCACC G^GT[ACGT]ACC BstPI 75 GGTCACC G^GT[ACGT]ACC DpnII 367 GATC ^GATC DraII 816 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 816 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 75 GGTCACC G^GT[ACGT]ACC EcoRI 3 GAATTC G^AATTC EcoRV 895 GATATC GAT^ATC FauI 559 CCCGC CCCGC(4/6) HincII 171 GTCAAC GT[CT]^[GA]AC HindII 171 GTCAAC GT[CT]^[GA]AC MamI 1270 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 367 GATC ^GATC NdeII 367 GATC ^GATC PflMI 494 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 876 TCATGA T^CATGA RsaI 952 GTAC GT^AC Sau3AI 367 GATC ^GATC SspI 1019 AATATT AAT^ATT TaqI 630 TCGA T^CGA TthHB8I 630 TCGA T^CGA Van91I 494 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 300 GAAGATGTTC AspI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACTGTC BcnI 72 CCCGG CC^[GC]GG 363 CCGGG BsgI 778 CTGCAC GTGCAG(16/14) 812 GTGCAG HapII 73 CCGG C^CGG 363 CCGG HindIII 415 AAGCTT A^AGCTT 449 AAGCTT HpaII 73 CCGG C^CGG 363 CCGG MaeIII 76 GTCAC ^GT[ACGT]AC 938 GTGAC MspI 73 CCGG C^CGG 363 CCGG NciI 72 CCCGG CC^[GC]GG 363 CCGGG NlaIV 323 GGAGCC GG[ACGT]^[ACGT]CC 817 GGGCCC NspI 782 ACATGC [GA]CATG^[CT] 923 GCATGT PflFI 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACTGTC SacI 258 GAGCTC GAGCT^C 805 GAGCTC StuI 106 AGGCCT AGG^CCT 1330 AGGCCT Tsp45I 76 GTCAC ^GT[GC]AC 938 GTGAC Tth111I 70 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC 374 GACACTGTC XmnI 57 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 300 GAAGATGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AluI AlwNI ApoI AspHI BanII BbvI BfaI BmyI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MboII MnlI MseI MslI MvaI MwoI NlaIII PleI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI