Restriction map for
pFN21AE0567
(Linear) (Six-base) MAPSORT of: KIEE0567.seq Check: 6743 from: 1 to: 765
KIEE0567.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 42 469 765
Size: 42 427 296
Fragments arranged by size:
427 296 42
AleI CACnn'nnGTG
Cuts at: 0 145 765
Size: 145 620
AlwNI CAG_nnn'CTG
Cuts at: 0 35 107 167 197 221 765
Size: 35 72 60 30 24 544
Fragments arranged by size:
544 72 60 35 30 24
BanI G'GyrC_C
Cuts at: 0 94 765
Size: 94 671
BanII G_rGCy'C
Cuts at: 0 86 191 765
Size: 86 105 574
Fragments arranged by size:
574 105 86
BglI GCCn_nnn'nGGC
Cuts at: 0 206 765
Size: 206 559
Bme1580I G_kGCm'C
Cuts at: 0 114 305 335 458 765
Size: 114 191 30 123 307
Fragments arranged by size:
307 191 123 114 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 139 765
Size: 139 626
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 765
Size: 492 273
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 765
Size: 12 753
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 264 513 654 672 675 688 765
Size: 264 249 141 18 3 13 77
Fragments arranged by size:
264 249 141 77 18 13 3
BseYI C'CCAG_C
Cuts at: 0 100 114 190 723 765
Size: 100 14 76 533 42
Fragments arranged by size:
533 100 76 42 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 21 123 195 207 252 501 642 765
Size: 21 102 72 12 45 249 141 123
Fragments arranged by size:
249 141 123 102 72 45 21 12
BsiHKAI G_wGCw'C
Cuts at: 0 86 718 765
Size: 86 632 47
Fragments arranged by size:
632 86 47
Bsp1286I G_dGCh'C
Cuts at: 0 86 114 191 305 335 458 718 765
Size: 86 28 77 114 30 123 260 47
Fragments arranged by size:
260 123 114 86 77 47 30 28
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 42 469 765
Size: 42 427 296
Fragments arranged by size:
427 296 42
BsrFI r'CCGG_y
Cuts at: 0 258 507 765
Size: 258 249 258
Fragments arranged by size:
258 258 249
BssSI C'ACGA_G
Cuts at: 0 231 765
Size: 231 534
Bsu36I CC'TnA_GG
Cuts at: 0 744 765
Size: 744 21
EaeI y'GGCC_r
Cuts at: 0 2 765
Size: 2 763
EarI CTCTTCn'nnn_
Cuts at: 0 402 483 765
Size: 402 81 282
Fragments arranged by size:
402 282 81
EcoICRI GAG'CTC
Cuts at: 0 84 765
Size: 84 681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 765
Size: 12 753
HaeII r_GCGC'y
Cuts at: 0 35 197 765
Size: 35 162 568
Fragments arranged by size:
568 162 35
MslI CAynn'nnrTG
Cuts at: 0 145 765
Size: 145 620
MspA1I CmG'CkG
Cuts at: 0 104 765
Size: 104 661
NaeI GCC'GGC
Cuts at: 0 260 509 765
Size: 260 249 256
Fragments arranged by size:
260 256 249
NgoMIV G'CCGG_C
Cuts at: 0 258 507 765
Size: 258 249 258
Fragments arranged by size:
258 258 249
PflMI CCAn_nnn'nTGG
Cuts at: 0 47 765
Size: 47 718
PshAI GACnn'nnGTC
Cuts at: 0 133 765
Size: 133 632
PstI C_TGCA'G
Cuts at: 0 676 736 765
Size: 676 60 29
Fragments arranged by size:
676 60 29
PvuII CAG'CTG
Cuts at: 0 104 765
Size: 104 661
SacI G_AGCT'C
Cuts at: 0 86 765
Size: 86 679
SfcI C'TryA_G
Cuts at: 0 672 732 765
Size: 672 60 33
Fragments arranged by size:
672 60 33
StuI AGG'CCT
Cuts at: 0 292 765
Size: 292 473
Enzymes that do cut:
AarI AleI AlwNI BanI BanII BglI Bme1580I BmrI
BplI BpmI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BssSI Bsu36I EaeI EarI EcoICRI Eco57MI HaeII
MslI MspA1I NaeI NgoMIV PflMI PshAI PstI PvuII
SacI SfcI StuI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI