################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI Aor51HI AscI Asp700I Asp718I AspEI AviII AvrII BamHI BbvCI BciVI BclI BfrI BlnI BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HpaI KpnI MamI MluI MroI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI Psp1406I PvuI RsrII SalI SapI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AccI 2761 GTCTAC GT^[AC][GT]AC AflIII 1787 ACGTGT A^C[GA][CT]GT AgeI 69 ACCGGT A^CCGGT Alw44I 982 GTGCAC G^TGCAC ApaLI 982 GTGCAC G^TGCAC BalI 1121 TGGCCA TGG^CCA BfaI 2320 CTAG C^TAG BglII 2606 AGATCT A^GATCT BsmI 894 GCATTC GAATGC(1/-1) BspMI 2027 ACCTGC ACCTGC(4/8) BsrDI 660 CATTGC GCAATG(2/0) BstAPI 2031 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC FseI 1285 GGCCGGCC GGCCGG^CC HindIII 60 AAGCTT A^AGCTT KspI 171 CCGCGG CCGC^GG MaeI 2320 CTAG C^TAG MfeI 215 CAATTG C^AATTG MluNI 1121 TGGCCA TGG^CCA MscI 1121 TGGCCA TGG^CCA MunI 215 CAATTG C^AATTG PflMI 300 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 69 ACCGGT A^CCGGT SacII 171 CCGCGG CCGC^GG Van91I 300 CCACAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AseI 961 ATTAAT AT^TAAT 1093 ATTAAT AsnI 961 ATTAAT AT^TAAT 1093 ATTAAT AspI 844 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1056 GACTCGGTC BanI 166 GGCGCC G^G[CT][GA]CC 1566 GGCGCC BbeI 166 GGCGCC GGCGC^C 1566 GGCGCC BbrPI 1786 CACGTG CAC^GTG 1963 CACGTG BcgI 1598 GCAGCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1724 GCACCTACTTCG BglI 1286 GCCGGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1412 GCCATGGAGGC BsaI 1558 GAGACC GGTCTC(1/5) 2574 GAGACC BstYI 497 GGATCT [GA]^GATC[CT] 2606 AGATCT EarI 2659 CTCTTC CTCTTC(1/4) 340 GAAGAG HgaI 912 GACGC GACGC(5/10) 867 GCGTC KasI 166 GGCGCC G^GCGCC 1566 GGCGCC Ksp632I 2659 CTCTTC CTCTTC(1/4) 340 GAAGAG MflI 497 GGATCT [GA]^GATC[CT] 2606 AGATCT NaeI 1286 GCCGGC GCC^GGC 1421 GCCGGC NarI 166 GGCGCC GG^CGCC 1566 GGCGCC NdeI 432 CATATG CA^TATG 2369 CATATG NgoMIV 1286 GCCGGC G^CCGGC 1421 GCCGGC PflFI 844 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1056 GACTCGGTC PmaCI 1786 CACGTG CAC^GTG 1963 CACGTG PmlI 1786 CACGTG CAC^GTG 1963 CACGTG PshBI 961 ATTAAT AT^TAAT 1093 ATTAAT SbfI 477 CCTGCAGG CCTGCA^GG 1764 CCTGCAGG SfoI 166 GGCGCC GGC^GCC 1566 GGCGCC SphI 1145 GCATGC GCATG^C 1487 GCATGC Sse8387I 477 CCTGCAGG CCTGCA^GG 1764 CCTGCAGG StuI 829 AGGCCT AGG^CCT 1005 AGGCCT Tsp45I 1550 GTGAC ^GT[GC]AC 2273 GTGAC Tth111I 844 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1056 GACTCGGTC XhoII 497 GGATCT [GA]^GATC[CT] 2606 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XmaCI XmaI