Restriction map for
pFN21AE4248
(Circular) (Six-base) MAPSORT of: pFN21AE4248.seq Check: 5983 from: 1 to: 7537
pFN21AE4248 7537 bp
With 182 enzymes: SgfI *
March 28, 2012 13:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5347 278
Size: 53 83 186 936 3811 2468
Fragments arranged by size:
3811 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4767 4880 1937
Size: 2830 113 4594
Fragments arranged by size:
4594 2830 113
Acc65I G'GTAC_C
Cuts at: 4858 4858
Size: 7537
AclI AA'CG_TT
Cuts at: 1563 5664 6037 1563
Size: 4101 373 3063
Fragments arranged by size:
4101 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2026 2907 3254 3921 4004 5600 6984 7252
Size: 394 881 347 667 83 1596 1384 268
Cuts at: 7252 1632
Size: 1917
Fragments arranged by size:
1917 1596 1384 881 667 394 347 268
83
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7340
AflIII A'CryG_T
Cuts at: 1203 3792 6436 7413 1203
Size: 2589 2644 977 1327
Fragments arranged by size:
2644 2589 1327 977
AgeI A'CCGG_T
Cuts at: 2074 6368 2074
Size: 4294 3243
AhdI GACnn_n'nnGTC
Cuts at: 6266 6266
Size: 7537
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3824 1184
Size: 2640 4897
AlwNI CAG_nnn'CTG
Cuts at: 3436 4066 4690 5280 6852 3436
Size: 630 624 590 1572 4121
Fragments arranged by size:
4121 1572 630 624 590
ApaI G_GGCC'C
Cuts at: 2578 2956 3349 3385 2578
Size: 378 393 36 6730
Fragments arranged by size:
6730 393 378 36
ApaLI G'TGCA_C
Cuts at: 2987 5594 6750 2987
Size: 2607 1156 3774
Fragments arranged by size:
3774 2607 1156
ApoI r'AATT_y
Cuts at: 1493 2092 2567 2588 2779 4399 4846 5072 5224
Size: 599 475 21 191 1620 447 226 152
Cuts at: 5224 7333 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1620 599 475 447 226 191
152 21
AseI AT'TA_AT
Cuts at: 160 1032 2967 3099 6091 160
Size: 872 1935 132 2992 1606
Fragments arranged by size:
2992 1935 1606 872 132
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7537
AvaI C'yCGr_G
Cuts at: 1870 1943 2367 3364 3717 4862 1870
Size: 73 424 997 353 1145 4545
Fragments arranged by size:
4545 1145 997 424 353 73
BamHI G'GATC_C
Cuts at: 4867 5234 4867
Size: 367 7170
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 3571 4858 6313 618
Size: 325 217 627 384 1400 1287 1455 1842
Fragments arranged by size:
1842 1455 1400 1287 627 384 325 217
BanII G_rGCy'C
Cuts at: 1391 2578 2792 2956 3349 3385 3866 4323 4856
Size: 1187 214 164 393 36 481 457 533
Cuts at: 4856 1391
Size: 4072
Fragments arranged by size:
4072 1187 533 481 457 393 214 164
36
BbeI G_GCGC'C
Cuts at: 2175 3575 2175
Size: 1400 6137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2363 3909 4066 4827 928
Size: 971 464 1546 157 761 3638
Fragments arranged by size:
3638 1546 971 761 464 157
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3592 3626 3718 3752 5728 5762 1903
Size: 34 1655 34 92 34 1976 34 3678
Fragments arranged by size:
3678 1976 1655 92 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5429 6639 5429
Size: 1210 6327
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7537
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3297 3423 6148
Size: 107 122 71 631 605 1625 126 2725
Cuts at: 6148 136
Size: 1525
Fragments arranged by size:
2725 1625 1525 631 605 126 122 107
71
BglII A'GATC_T
Cuts at: 4611 7532 4611
Size: 2921 4616
BlpI GC'TnA_GC
Cuts at: 2402 2748 3437 4430 4954 2402
Size: 346 689 993 524 4985
Fragments arranged by size:
4985 993 689 524 346
Bme1580I G_kGCm'C
Cuts at: 1790 2368 2578 2956 2991 3326 3349 3385 3969
Size: 578 210 378 35 335 23 36 584
Cuts at: 3969 5598 6754 1790
Size: 1629 1156 2573
Fragments arranged by size:
2573 1629 1156 584 578 378 335 210
36 35 23
BmgBI CAC'GTC
Cuts at: 1318 2353 1318
Size: 1035 6502
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4267 5268 6226
Size: 416 53 83 399 24 2850 1001 958
Cuts at: 6226 442
Size: 1753
Fragments arranged by size:
2850 1753 1001 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3513 4231 3513
Size: 718 6819
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2107 2806 2951 3335 3497 3554 6197 1239
Size: 868 699 145 384 162 57 2643 2579
Fragments arranged by size:
2643 2579 868 699 384 162 145 57
Bpu10I CC'TnA_GC
Cuts at: 2632 2632
Size: 7537
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2007 2567 3301 3944 4007 5662 6527 6825 2007
Size: 560 734 643 63 1655 865 298 2719
Fragments arranged by size:
2719 1655 865 734 643 560 298 63
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3557 4573 6200 882
Size: 617 2058 1016 1627 2219
Fragments arranged by size:
2219 2058 1627 1016 617
BsaAI yAC'GTr
Cuts at: 493 1204 3760 3793 3970 5328 493
Size: 711 2556 33 177 1358 2702
Fragments arranged by size:
2702 2556 1358 711 177 33
BsaBI GATnn'nnATC
Cuts at: 7531 7531
Size: 7537
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 2918 3572 5344
Size: 53 83 186 936 639 746 654 1772
Cuts at: 5344 5726 6361 275
Size: 382 635 1451
Fragments arranged by size:
1772 1451 936 746 654 639 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2074 3486 4485 5970 6368 6642 6789 7200 2074
Size: 1412 999 1485 398 274 147 411 2411
Fragments arranged by size:
2411 1485 1412 999 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2375 2405 3019 3049 1178
Size: 30 165 30 972 30 614 30 5666
Fragments arranged by size:
5666 972 614 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2411 2477 2480 3454 4661 1186
Size: 1225 66 3 974 1207 4062
Fragments arranged by size:
4062 1225 1207 974 66 3
BseYI C'CCAG_C
Cuts at: 2143 2490 2794 3319 3392 3636 4066 6740 7236
Size: 347 304 525 73 244 430 2674 496
Cuts at: 7236 2143
Size: 2444
Fragments arranged by size:
2674 2444 525 496 430 347 304 244
73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2149 2956 3770 4715 1163
Size: 573 413 807 814 945 3985
Fragments arranged by size:
3985 945 814 807 573 413
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3023 3292 3296 3487 4987 5748
Size: 275 186 1022 269 4 191 1500 761
Cuts at: 5748 6776 1540
Size: 1028 2301
Fragments arranged by size:
2301 1500 1028 1022 761 275 269 191
186 4
BsiHKAI G_wGCw'C
Cuts at: 2792 2991 3029 3866 4323 4384 5598 5683 6754
Size: 199 38 837 457 61 1214 85 1071
Cuts at: 6754 2792
Size: 3575
Fragments arranged by size:
3575 1214 1071 837 457 199 85 61
38
BsmI GAATG_Cn'
Cuts at: 2899 5048 5141 2899
Size: 2149 93 5295
Fragments arranged by size:
5295 2149 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2368 2578 2792 2956 2991 3029 3326
Size: 399 578 210 214 164 35 38 297
Cuts at: 3326 3349 3385 3866 3969 4323 4384 4856 5598
Size: 23 36 481 103 354 61 472 742
Cuts at: 5598 5683 6754 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 578 481 472 399 354
297 214 210 164 103 85 61 38
36 35 23
BspEI T'CCGG_A
Cuts at: 7200 7200
Size: 7537
BspHI T'CATG_A
Cuts at: 3672 3831 4125 5426 7156 3672
Size: 159 294 1301 1730 4053
Fragments arranged by size:
4053 1730 1301 294 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4041 4892 844
Size: 3197 851 3489
Fragments arranged by size:
3489 3197 851
BsrBI CCG'CTC
Cuts at: 1394 1823 3058 3285 3654 5424 1394
Size: 429 1235 227 369 1770 3507
Fragments arranged by size:
3507 1770 1235 429 369 227
BsrDI GCAATG_nn'
Cuts at: 65 1248 2664 6032 6206 65
Size: 1183 1416 3368 174 1396
Fragments arranged by size:
3368 1416 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2074 2415 3291 3426 6181 6368 1700
Size: 110 264 341 876 135 2755 187 2869
Fragments arranged by size:
2869 2755 876 341 264 187 135 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7537
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7537
BssSI C'ACGA_G
Cuts at: 5597 6609 5597
Size: 1012 6525
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4042 794
Size: 3248 4289
BstBI TT'CG_AA
Cuts at: 6341 6341
Size: 7537
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7537
BstYI r'GATC_y
Cuts at: 1972 2502 4611 4867 5234 5619 5636 7088 7174
Size: 530 2109 256 367 385 17 1452 86
Cuts at: 7174 7186 7532 1972
Size: 12 346 1977
Fragments arranged by size:
2109 1977 1452 530 385 367 346 256
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3354 4454 4644 3354
Size: 1100 190 6247
Fragments arranged by size:
6247 1100 190
BtgI C'CryG_G
Cuts at: 513 1065 2166 2176 3418 3567 513
Size: 552 1101 10 1242 149 4483
Fragments arranged by size:
4483 1242 1101 552 149 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4317 487
Size: 683 56 3091 3707
Fragments arranged by size:
3707 3091 683 56
BtsI GCAGTG_nn'
Cuts at: 2137 4466 5053 5847 5867 2137
Size: 2329 587 794 20 3807
Fragments arranged by size:
3807 2329 794 587 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7537
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2311 2346 529
Size: 35 1747 35 5720
Fragments arranged by size:
5720 1747 35 35
DraI TTT'AAA
Cuts at: 4841 4841
Size: 7537
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6544 809
Size: 507 5228 1802
Fragments arranged by size:
5228 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3020 3126 3289
Size: 54 1425 50 275 18 1190 106 163
Cuts at: 3289 3293 3999 4984 5873 7502 8
Size: 4 706 985 889 1629 43
Fragments arranged by size:
1629 1425 1190 985 889 706 275 163
106 54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3020 3289 3293 4984 1537
Size: 275 1208 269 4 1691 4090
Fragments arranged by size:
4090 1691 1208 275 269 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2340 4670 5467 1353
Size: 987 2330 797 3423
Fragments arranged by size:
3423 2330 987 797
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2543 3530 4739 6124 6498 6644
Size: 1064 138 954 987 1209 1385 374 146
Cuts at: 6644 387
Size: 1280
Fragments arranged by size:
1385 1280 1209 1064 987 954 374 146
138
EcoICRI GAG'CTC
Cuts at: 2790 3864 4321 2790
Size: 1074 457 6006
Fragments arranged by size:
6006 1074 457
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2026 2107 2806 2907 2951 3254 3335
Size: 393 394 81 699 101 44 303 81
Cuts at: 3335 3497 3554 3921 4004 5600 6197 6984 7252
Size: 162 57 367 83 1596 597 787 268
Cuts at: 7252 1239
Size: 1524
Fragments arranged by size:
1596 1524 787 699 597 394 393 367
303 268 162 101 83 81 81 57
44
EcoO109I rG'GnC_Cy
Cuts at: 2479 2574 2575 2952 2953 2998 3081 3345 3346
Size: 95 1 377 1 45 83 264 1
Cuts at: 3346 3381 3382 4639 2479
Size: 35 1 1257 5377
Fragments arranged by size:
5377 1257 377 264 95 83 45 35
1 1 1 1
EcoRI G'AATT_C
Cuts at: 4846 5224 4846
Size: 378 7159
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3964 3964
Size: 7537
FseI GG_CCGG'CC
Cuts at: 3295 3295
Size: 7537
FspI TGC'GCA
Cuts at: 6043 6043
Size: 7537
HaeII r_GCGC'y
Cuts at: 1678 2175 3575 4236 6684 1678
Size: 497 1400 661 2448 2531
Fragments arranged by size:
2531 2448 1400 661 497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 4572 4604 6178
Size: 32 135 32 803 32 2165 32 1574
Cuts at: 6178 6210 6252 6284 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2165 1574 803 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 3166 4546 4672 4881 5127
Size: 988 281 334 894 1380 126 209 246
Cuts at: 5127 669
Size: 3079
Fragments arranged by size:
3079 1380 988 894 334 281 246 209
126
HindIII A'AGCT_T
Cuts at: 748 2065 748
Size: 1317 6220
HpaI GTT'AAC
Cuts at: 5127 5127
Size: 7537
KasI G'GCGC_C
Cuts at: 2171 3571 2171
Size: 1400 6137
KpnI G_GTAC'C
Cuts at: 4862 4862
Size: 7537
MfeI C'AATT_G
Cuts at: 2220 5136 2220
Size: 2916 4621
MluI A'CGCG_T
Cuts at: 7413 7413
Size: 7537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 2095 2418 2721 2871 3492 6651 6835
Size: 684 106 323 303 150 621 3159 184
Cuts at: 6835 1305
Size: 2007
Fragments arranged by size:
3159 2007 684 621 323 303 184 150
106
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3128 10
Size: 54 1425 343 1296 4419
Fragments arranged by size:
4419 1425 1296 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2325 3806 4130 4202 5495 5854 6013
Size: 648 1159 1481 324 72 1293 359 159
Cuts at: 6013 518
Size: 2042
Fragments arranged by size:
2042 1481 1293 1159 648 359 324 159
72
MspA1I CmG'CkG
Cuts at: 1606 2178 2297 2449 2798 3329 3433 3640 4690
Size: 572 119 152 349 531 104 207 1050
Cuts at: 4690 4953 5630 6778 7023 1606
Size: 263 677 1148 245 2120
Fragments arranged by size:
2120 1148 1050 677 572 531 349 263
245 207 152 119 104
NaeI GCC'GGC
Cuts at: 3293 3428 3293
Size: 135 7402
NarI GG'CG_CC
Cuts at: 2172 3572 2172
Size: 1400 6137
NcoI C'CATG_G
Cuts at: 513 1065 2166 3418 3567 513
Size: 552 1101 1252 149 4483
Fragments arranged by size:
4483 1252 1101 552 149
NdeI CA'TA_TG
Cuts at: 387 2438 4375 387
Size: 2051 1937 3549
Fragments arranged by size:
3549 2051 1937
NgoMIV G'CCGG_C
Cuts at: 3291 3426 3291
Size: 135 7402
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7537
NotI GC'GGCC_GC
Cuts at: 4984 4984
Size: 7537
NspI r_CATG'y
Cuts at: 1132 1229 3154 3496 3811 4285 4895 6440 1132
Size: 97 1925 342 315 474 610 1545 2229
Fragments arranged by size:
2229 1925 1545 610 474 342 315 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7537
PciI A'CATG_T
Cuts at: 6436 6436
Size: 7537
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 1108
Size: 1203 6334
PmeI GTTT'AAAC
Cuts at: 4841 4841
Size: 7537
PmlI CAC'GTG
Cuts at: 3793 3970 3793
Size: 177 7360
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5606 5638 7119 7151 5606
Size: 32 1481 32 5992
Fragments arranged by size:
5992 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2998 4639 2479
Size: 519 1641 5377
Fragments arranged by size:
5377 1641 519
PshAI GACnn'nnGTC
Cuts at: 1613 2267 3839 4262 1613
Size: 654 1572 423 4888
Fragments arranged by size:
4888 1572 654 423
PsiI TTA'TAA
Cuts at: 2888 5107 2888
Size: 2219 5318
PspOMI G'GGCC_C
Cuts at: 2574 2952 3345 3381 2574
Size: 378 393 36 6730
Fragments arranged by size:
6730 393 378 36
PstI C_TGCA'G
Cuts at: 830 2448 2487 3645 3774 3816 4038 4889 830
Size: 1618 39 1158 129 42 222 851 3478
Fragments arranged by size:
3478 1618 1158 851 222 129 42 39
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7537
PvuII CAG'CTG
Cuts at: 2449 2798 3329 3640 4690 2449
Size: 349 531 311 1050 5296
Fragments arranged by size:
5296 1050 531 349 311
SacI G_AGCT'C
Cuts at: 2792 3866 4323 2792
Size: 1074 457 6006
Fragments arranged by size:
6006 1074 457
SacII CC_GC'GG
Cuts at: 2179 2179
Size: 7537
SalI G'TCGA_C
Cuts at: 1936 4879 1936
Size: 2943 4594
SanDI GG'GwC_CC
Cuts at: 4639 4639
Size: 7537
SbfI CC_TGCA'GG
Cuts at: 2487 3774 4889 2487
Size: 1287 1115 5135
Fragments arranged by size:
5135 1287 1115
ScaI AGT'ACT
Cuts at: 5785 7497 5785
Size: 1712 5825
SfcI C'TryA_G
Cuts at: 826 1045 2444 2483 3641 3770 3812 4034 4885
Size: 219 1399 39 1158 129 42 222 851
Cuts at: 4885 6020 6701 6892 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1399 1158 1135 851 681 222 219
191 129 42 39
SfoI GGC'GCC
Cuts at: 2173 3573 2173
Size: 1400 6137
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7537
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7537
SmaI CCC'GGG
Cuts at: 1872 2369 3366 3719 4864 1872
Size: 497 997 353 1145 4545
Fragments arranged by size:
4545 1145 997 497 353
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2022 2582 3280 3959 4022 5641
Size: 197 926 79 560 698 679 63 1619
Cuts at: 5641 6542 6804 820
Size: 901 262 1553
Fragments arranged by size:
1619 1553 926 901 698 679 560 262
197 79 63
SnaBI TAC'GTA
Cuts at: 493 5328 493
Size: 4835 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7537
SphI G_CATG'C
Cuts at: 1132 3154 3496 4895 1132
Size: 2022 342 1399 3774
Fragments arranged by size:
3774 2022 1399 342
SspI AAT'ATT
Cuts at: 5 52 5461 5
Size: 47 5409 2081
Fragments arranged by size:
5409 2081 47
StuI AGG'CCT
Cuts at: 1517 2836 3012 1517
Size: 1319 176 6042
Fragments arranged by size:
6042 1319 176
StyI C'CwwG_G
Cuts at: 513 1065 2166 3418 3567 4976 513
Size: 552 1101 1252 149 1409 3074
Fragments arranged by size:
3074 1409 1252 1101 552 149
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2444 3054 3091 5564 5581 5734 5919
Size: 1255 864 610 37 2473 17 153 185
Cuts at: 5919 6364 325
Size: 445 1498
Fragments arranged by size:
2473 1498 1255 864 610 445 185 153
37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4308 4650 5783
Size: 274 80 33 51 1443 2331 342 1133
Cuts at: 5783 7495 96
Size: 1712 138
Fragments arranged by size:
2331 1712 1443 1133 342 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3019 3051 7497 7529 3019
Size: 32 4446 32 3027
Fragments arranged by size:
4446 3027 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2852 3064 1316
Size: 1536 212 5789
Fragments arranged by size:
5789 1536 212
XbaI T'CTAG_A
Cuts at: 4873 4873
Size: 7537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3002 3176 3529 4498 3002
Size: 174 353 969 6041
Fragments arranged by size:
6041 969 353 174
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7537
XmaI C'CCGG_G
Cuts at: 1870 2367 3364 3717 4862 1870
Size: 497 997 353 1145 4545
Fragments arranged by size:
4545 1145 997 497 353
XmnI GAAnn'nnTTC
Cuts at: 5666 5666
Size: 7537
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5345 276
Size: 53 83 186 936 3811 2468
Fragments arranged by size:
3811 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FspAI
NruI NsiI PacI PfoI PspXI PsrI RsrII SapI
SexAI SfiI SrfI SwaI