(Linear) (Six-base) MAPSORT of: KIEE4248.seq Check: 6391 from: 1 to: 2832 KIEE4248.orf With 182 enzymes: SgfI * March 22, 2012 17:38 .. AccI GT'mk_AC Cuts at: 0 2762 2832 Size: 2762 70 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 902 1249 1916 1999 2832 Size: 21 881 347 667 83 833 Fragments arranged by size: 881 833 667 347 83 21 AflIII A'CryG_T Cuts at: 0 1787 2832 Size: 1787 1045 AgeI A'CCGG_T Cuts at: 0 69 2832 Size: 69 2763 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1819 2832 Size: 1819 1013 AlwNI CAG_nnn'CTG Cuts at: 0 1431 2061 2685 2832 Size: 1431 630 624 147 Fragments arranged by size: 1431 630 624 147 ApaI G_GGCC'C Cuts at: 0 573 951 1344 1380 2832 Size: 573 378 393 36 1452 Fragments arranged by size: 1452 573 393 378 36 ApaLI G'TGCA_C Cuts at: 0 982 2832 Size: 982 1850 ApoI r'AATT_y Cuts at: 0 87 562 583 774 2394 2832 Size: 87 475 21 191 1620 438 Fragments arranged by size: 1620 475 438 191 87 21 AseI AT'TA_AT Cuts at: 0 962 1094 2832 Size: 962 132 1738 Fragments arranged by size: 1738 962 132 AvaI C'yCGr_G Cuts at: 0 362 1359 1712 2832 Size: 362 997 353 1120 Fragments arranged by size: 1120 997 362 353 BanI G'GyrC_C Cuts at: 0 166 1566 2832 Size: 166 1400 1266 Fragments arranged by size: 1400 1266 166 BanII G_rGCy'C Cuts at: 0 573 787 951 1344 1380 1861 2318 2832 Size: 573 214 164 393 36 481 457 514 Fragments arranged by size: 573 514 481 457 393 214 164 36 BbeI G_GCGC'C Cuts at: 0 170 1570 2832 Size: 170 1400 1262 Fragments arranged by size: 1400 1262 170 BbsI GAAGACnn'nnnn_ Cuts at: 0 358 1904 2061 2822 2832 Size: 358 1546 157 761 10 Fragments arranged by size: 1546 761 358 157 10 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1587 1621 1713 1747 2832 Size: 1587 34 92 34 1085 Fragments arranged by size: 1587 1085 92 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1292 1418 2832 Size: 1292 126 1414 Fragments arranged by size: 1414 1292 126 BglII A'GATC_T Cuts at: 0 2606 2832 Size: 2606 226 BlpI GC'TnA_GC Cuts at: 0 397 743 1432 2425 2832 Size: 397 346 689 993 407 Fragments arranged by size: 993 689 407 397 346 Bme1580I G_kGCm'C Cuts at: 0 363 573 951 986 1321 1344 1380 1964 Size: 363 210 378 35 335 23 36 584 Cuts at: 1964 2832 Size: 868 Fragments arranged by size: 868 584 378 363 335 210 36 35 23 BmgBI CAC'GTC Cuts at: 0 348 2832 Size: 348 2484 BmrI ACTGGGnnnn_n' Cuts at: 0 2262 2832 Size: 2262 570 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1508 2226 2832 Size: 1508 718 606 Fragments arranged by size: 1508 718 606 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 102 801 946 1330 1492 1549 2832 Size: 102 699 145 384 162 57 1283 Fragments arranged by size: 1283 699 384 162 145 102 57 Bpu10I CC'TnA_GC Cuts at: 0 627 2832 Size: 627 2205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2 562 1296 1939 2002 2832 Size: 2 560 734 643 63 830 Fragments arranged by size: 830 734 643 560 63 2 BsaI GGTCTCn'nnnn_ Cuts at: 0 1552 2568 2832 Size: 1552 1016 264 Fragments arranged by size: 1552 1016 264 BsaAI yAC'GTr Cuts at: 0 1755 1788 1965 2832 Size: 1755 33 177 867 Fragments arranged by size: 1755 867 177 33 BsaHI Gr'CG_yC Cuts at: 0 167 913 1567 2832 Size: 167 746 654 1265 Fragments arranged by size: 1265 746 654 167 BsaWI w'CCGG_w Cuts at: 0 69 1481 2480 2832 Size: 69 1412 999 352 Fragments arranged by size: 1412 999 352 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 370 400 1014 1044 2832 Size: 370 30 614 30 1788 Fragments arranged by size: 1788 614 370 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 406 472 475 1449 2656 2832 Size: 406 66 3 974 1207 176 Fragments arranged by size: 1207 974 406 176 66 3 BseYI C'CCAG_C Cuts at: 0 138 485 789 1314 1387 1631 2061 2832 Size: 138 347 304 525 73 244 430 771 Fragments arranged by size: 771 525 430 347 304 244 138 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 951 1765 2710 2832 Size: 144 807 814 945 122 Fragments arranged by size: 945 814 807 144 122 BsiEI CG_ry'CG Cuts at: 0 1018 1287 1291 1482 2832 Size: 1018 269 4 191 1350 Fragments arranged by size: 1350 1018 269 191 4 BsiHKAI G_wGCw'C Cuts at: 0 787 986 1024 1861 2318 2379 2832 Size: 787 199 38 837 457 61 453 Fragments arranged by size: 837 787 457 453 199 61 38 BsmI GAATG_Cn' Cuts at: 0 894 2832 Size: 894 1938 Bsp1286I G_dGCh'C Cuts at: 0 363 573 787 951 986 1024 1321 1344 Size: 363 210 214 164 35 38 297 23 Cuts at: 1344 1380 1861 1964 2318 2379 2832 Size: 36 481 103 354 61 453 Fragments arranged by size: 481 453 363 354 297 214 210 164 103 61 38 36 35 23 BspHI T'CATG_A Cuts at: 0 1667 1826 2120 2832 Size: 1667 159 294 712 Fragments arranged by size: 1667 712 294 159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2036 2832 Size: 2036 796 BsrBI CCG'CTC Cuts at: 0 1053 1280 1649 2832 Size: 1053 227 369 1183 Fragments arranged by size: 1183 1053 369 227 BsrDI GCAATG_nn' Cuts at: 0 659 2832 Size: 659 2173 BsrFI r'CCGG_y Cuts at: 0 69 410 1286 1421 2832 Size: 69 341 876 135 1411 Fragments arranged by size: 1411 876 341 135 69 BstAPI GCAn_nnn'nTGC Cuts at: 0 2037 2832 Size: 2037 795 BstYI r'GATC_y Cuts at: 0 497 2606 2832 Size: 497 2109 226 Fragments arranged by size: 2109 497 226 Bsu36I CC'TnA_GG Cuts at: 0 1349 2449 2639 2832 Size: 1349 1100 190 193 Fragments arranged by size: 1349 1100 193 190 BtgI C'CryG_G Cuts at: 0 161 171 1413 1562 2832 Size: 161 10 1242 149 1270 Fragments arranged by size: 1270 1242 161 149 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2312 2832 Size: 2312 520 BtsI GCAGTG_nn' Cuts at: 0 132 2461 2832 Size: 132 2329 371 Fragments arranged by size: 2329 371 132 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 306 341 2832 Size: 306 35 2491 Fragments arranged by size: 2491 306 35 EaeI y'GGCC_r Cuts at: 0 1015 1121 1284 1288 1994 2832 Size: 1015 106 163 4 706 838 Fragments arranged by size: 1015 838 706 163 106 4 EagI C'GGCC_G Cuts at: 0 1015 1284 1288 2832 Size: 1015 269 4 1544 Fragments arranged by size: 1544 1015 269 4 EarI CTCTTCn'nnn_ Cuts at: 0 335 2665 2832 Size: 335 2330 167 Fragments arranged by size: 2330 335 167 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 538 1525 2734 2832 Size: 538 987 1209 98 Fragments arranged by size: 1209 987 538 98 EcoICRI GAG'CTC Cuts at: 0 785 1859 2316 2832 Size: 785 1074 457 516 Fragments arranged by size: 1074 785 516 457 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 21 102 801 902 946 1249 1330 1492 Size: 21 81 699 101 44 303 81 162 Cuts at: 1492 1549 1916 1999 2832 Size: 57 367 83 833 Fragments arranged by size: 833 699 367 303 162 101 83 81 81 57 44 21 EcoO109I rG'GnC_Cy Cuts at: 0 474 569 570 947 948 993 1076 1340 Size: 474 95 1 377 1 45 83 264 Cuts at: 1340 1341 1376 1377 2634 2832 Size: 1 35 1 1257 198 Fragments arranged by size: 1257 474 377 264 198 95 83 45 35 1 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1959 2832 Size: 1959 873 FseI GG_CCGG'CC Cuts at: 0 1290 2832 Size: 1290 1542 HaeII r_GCGC'y Cuts at: 0 170 1570 2231 2832 Size: 170 1400 661 601 Fragments arranged by size: 1400 661 601 170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 370 402 2567 2599 2832 Size: 370 32 2165 32 233 Fragments arranged by size: 2165 370 233 32 32 HincII GTy'rAC Cuts at: 0 267 1161 2541 2667 2832 Size: 267 894 1380 126 165 Fragments arranged by size: 1380 894 267 165 126 HindIII A'AGCT_T Cuts at: 0 60 2832 Size: 60 2772 KasI G'GCGC_C Cuts at: 0 166 1566 2832 Size: 166 1400 1266 Fragments arranged by size: 1400 1266 166 MfeI C'AATT_G Cuts at: 0 215 2832 Size: 215 2617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 90 413 716 866 1487 2832 Size: 90 323 303 150 621 1345 Fragments arranged by size: 1345 621 323 303 150 90 MscI TGG'CCA Cuts at: 0 1123 2832 Size: 1123 1709 MslI CAynn'nnrTG Cuts at: 0 320 1801 2125 2197 2832 Size: 320 1481 324 72 635 Fragments arranged by size: 1481 635 324 320 72 MspA1I CmG'CkG Cuts at: 0 173 292 444 793 1324 1428 1635 2685 Size: 173 119 152 349 531 104 207 1050 Cuts at: 2685 2832 Size: 147 Fragments arranged by size: 1050 531 349 207 173 152 147 119 104 NaeI GCC'GGC Cuts at: 0 1288 1423 2832 Size: 1288 135 1409 Fragments arranged by size: 1409 1288 135 NarI GG'CG_CC Cuts at: 0 167 1567 2832 Size: 167 1400 1265 Fragments arranged by size: 1400 1265 167 NcoI C'CATG_G Cuts at: 0 161 1413 1562 2832 Size: 161 1252 149 1270 Fragments arranged by size: 1270 1252 161 149 NdeI CA'TA_TG Cuts at: 0 433 2370 2832 Size: 433 1937 462 Fragments arranged by size: 1937 462 433 NgoMIV G'CCGG_C Cuts at: 0 1286 1421 2832 Size: 1286 135 1411 Fragments arranged by size: 1411 1286 135 NspI r_CATG'y Cuts at: 0 1149 1491 1806 2280 2832 Size: 1149 342 315 474 552 Fragments arranged by size: 1149 552 474 342 315 PflMI CCAn_nnn'nTGG Cuts at: 0 306 2832 Size: 306 2526 PmlI CAC'GTG Cuts at: 0 1788 1965 2832 Size: 1788 177 867 Fragments arranged by size: 1788 867 177 PpuMI rG'GwC_Cy Cuts at: 0 474 993 2634 2832 Size: 474 519 1641 198 Fragments arranged by size: 1641 519 474 198 PshAI GACnn'nnGTC Cuts at: 0 262 1834 2257 2832 Size: 262 1572 423 575 Fragments arranged by size: 1572 575 423 262 PsiI TTA'TAA Cuts at: 0 883 2832 Size: 883 1949 PspOMI G'GGCC_C Cuts at: 0 569 947 1340 1376 2832 Size: 569 378 393 36 1456 Fragments arranged by size: 1456 569 393 378 36 PstI C_TGCA'G Cuts at: 0 443 482 1640 1769 1811 2033 2832 Size: 443 39 1158 129 42 222 799 Fragments arranged by size: 1158 799 443 222 129 42 39 PvuII CAG'CTG Cuts at: 0 444 793 1324 1635 2685 2832 Size: 444 349 531 311 1050 147 Fragments arranged by size: 1050 531 444 349 311 147 SacI G_AGCT'C Cuts at: 0 787 1861 2318 2832 Size: 787 1074 457 514 Fragments arranged by size: 1074 787 514 457 SacII CC_GC'GG Cuts at: 0 174 2832 Size: 174 2658 SanDI GG'GwC_CC Cuts at: 0 2634 2832 Size: 2634 198 SbfI CC_TGCA'GG Cuts at: 0 482 1769 2832 Size: 482 1287 1063 Fragments arranged by size: 1287 1063 482 SfcI C'TryA_G Cuts at: 0 439 478 1636 1765 1807 2029 2832 Size: 439 39 1158 129 42 222 803 Fragments arranged by size: 1158 803 439 222 129 42 39 SfoI GGC'GCC Cuts at: 0 168 1568 2832 Size: 168 1400 1264 Fragments arranged by size: 1400 1264 168 SmaI CCC'GGG Cuts at: 0 364 1361 1714 2832 Size: 364 997 353 1118 Fragments arranged by size: 1118 997 364 353 SmlI C'TyrA_G Cuts at: 0 17 577 1275 1954 2017 2832 Size: 17 560 698 679 63 815 Fragments arranged by size: 815 698 679 560 63 17 SphI G_CATG'C Cuts at: 0 1149 1491 2832 Size: 1149 342 1341 Fragments arranged by size: 1341 1149 342 StuI AGG'CCT Cuts at: 0 831 1007 2832 Size: 831 176 1825 Fragments arranged by size: 1825 831 176 StyI C'CwwG_G Cuts at: 0 161 1413 1562 2832 Size: 161 1252 149 1270 Fragments arranged by size: 1270 1252 161 149 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 439 1049 1086 2832 Size: 439 610 37 1746 Fragments arranged by size: 1746 610 439 37 TatI w'GTAC_w Cuts at: 0 2303 2645 2832 Size: 2303 342 187 Fragments arranged by size: 2303 342 187 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1014 1046 2832 Size: 1014 32 1786 Fragments arranged by size: 1786 1014 32 Tth111I GACn'n_nGTC Cuts at: 0 847 1059 2832 Size: 847 212 1773 Fragments arranged by size: 1773 847 212 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 997 1171 1524 2493 2832 Size: 997 174 353 969 339 Fragments arranged by size: 997 969 353 339 174 XmaI C'CCGG_G Cuts at: 0 362 1359 1712 2832 Size: 362 997 353 1120 Fragments arranged by size: 1120 997 362 353 Enzymes that do cut: AccI AcuI AflIII AgeI AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI FseI HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PflMI PmlI PpuMI PshAI PsiI PspOMI PstI PvuII SacI SacII SanDI SbfI SfcI SfoI SmaI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AhdI AleI AloI AscI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmtI BsaBI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I ClaI DraI DraIII DrdI EcoNI EcoRI EcoRV FspI FspAI HpaI KpnI MluI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmnI ZraI