################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BamHI BbsI BciVI BclI BfaI BfrI BlnI BlpI Bpu1102I BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI DraI DraIII DrdI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI SacI SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 904 GACGTC GACGT^C AfaI 368 GTAC GT^AC AhdI 266 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspEI 266 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 1204 GGCGCC GGCGC^C BbvCI 540 GCTGAGG CCTCAGC(-5/-2) BglI 1040 GCCGCCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 828 AGATCT A^GATCT BspHI 140 TCATGA T^CATGA BsrBI 1434 CCGCTC CCGCTC(-3/-3) BsrDI 866 GCAATG GCAATG(2/0) BssSI 592 CACGAG CACGAG(-5/-1) BstAPI 312 GCAGGCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 828 AGATCT [GA]^GATC[CT] CpoI 763 CGGTCCG CG^G[AT]CCG DpnII 829 GATC ^GATC Eam1105I 266 GACCCATCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HgaI 991 GACGC GACGC(5/10) KasI 1204 GGCGCC G^GCGCC MboI 829 GATC ^GATC MflI 828 AGATCT [GA]^GATC[CT] MslI 135 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1204 GGCGCC GG^CGCC NdeII 829 GATC ^GATC PshAI 452 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 140 TCATGA T^CATGA RsaI 368 GTAC GT^AC RsrII 763 CGGTCCG CG^G[AT]CCG Sau3AI 829 GATC ^GATC ScaI 367 AGTACT AGT^ACT SfoI 1204 GGCGCC GGC^GCC SmaI 261 CCCGGG CCC^GGG StuI 596 AGGCCT AGG^CCT TfiI 385 GATTC G^A[AT]TC XcmI 365 CCAGTACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 828 AGATCT [GA]^GATC[CT] XmaCI 261 CCCGGG C^CCGGG XmaI 261 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 357 CGCG CG^CG 1490 CGCG AcyI 904 GACGTC G[GA]^CG[CT]C 1204 GGCGCC AlwNI 290 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 989 CAGACGCTG AocI 287 CCTCAGG CC^T[ACGT]AGG 735 CCTCAGG Aor51HI 1247 AGCGCT AGC^GCT 1464 AGCGCT ApaI 1143 GGGCCC GGGCC^C 1226 GGGCCC BanI 709 GGCACC G^G[CT][GA]CC 1204 GGCGCC BbrPI 1177 CACGTG CAC^GTG 1257 CACGTG BcgI 594 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1176 GCACGTGGTTCG BsaAI 1177 CACGTG [CT]AC^GT[GA] 1257 CACGTG BsaHI 904 GACGTC G[GA]^CG[CT]C 1204 GGCGCC BspMI 294 ACCTGC ACCTGC(4/8) 1505 ACCTGC BstUI 357 CGCG CG^CG 1490 CGCG Bsu36I 287 CCTCAGG CC^T[ACGT]AGG 735 CCTCAGG Eco47III 1247 AGCGCT AGC^GCT 1464 AGCGCT Eco81I 287 CCTCAGG CC^T[ACGT]AGG 735 CCTCAGG Hin1I 904 GACGTC G[GA]^CG[CT]C 1204 GGCGCC MvnI 357 CGCG CG^CG 1490 CGCG NcoI 1100 CCATGG C^CATGG 1200 CCATGG PmaCI 1177 CACGTG CAC^GTG 1257 CACGTG PmlI 1177 CACGTG CAC^GTG 1257 CACGTG SmlI 250 CTCAAG C^T[CT][GA]AG 715 CTTGAG TaqI 185 TCGA T^CGA 1274 TCGA TthHB8I 185 TCGA T^CGA 1274 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AciI AluI AspHI AvaI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboII MluNI MnlI MscI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV NspI PleI PpuMI PstI PvuII Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI