(Circular) (Six-base) MAPSORT of: pFN21AE4124.seq Check: 1885 from: 1 to: 6241 pFN21AE4124 6241 bp With 182 enzymes: SgfI * March 28, 2012 13:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2913 4051 278 Size: 53 83 186 936 1377 1138 2468 Fragments arranged by size: 2468 1377 1138 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3584 1937 Size: 1647 4594 Acc65I G'GTAC_C Cuts at: 3562 3562 Size: 6241 AclI AA'CG_TT Cuts at: 1563 4368 4741 1563 Size: 2805 373 3063 Fragments arranged by size: 3063 2805 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4304 5688 5956 1632 Size: 2672 1384 268 1917 Fragments arranged by size: 2672 1917 1384 268 AfeI AGC'GCT Cuts at: 3254 3471 3254 Size: 217 6024 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6044 AflIII A'CryG_T Cuts at: 1203 5140 6117 1203 Size: 3937 977 1327 Fragments arranged by size: 3937 1327 977 AgeI A'CCGG_T Cuts at: 5072 5072 Size: 6241 AhdI GACnn_n'nnGTC Cuts at: 2276 4970 2276 Size: 2694 3547 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2609 2993 1184 Size: 1425 384 4432 Fragments arranged by size: 4432 1425 384 AlwNI CAG_nnn'CTG Cuts at: 2300 2999 3984 5556 2300 Size: 699 985 1572 2985 Fragments arranged by size: 2985 1572 985 699 ApaI G_GGCC'C Cuts at: 3152 3235 3152 Size: 83 6158 ApaLI G'TGCA_C Cuts at: 4298 5454 4298 Size: 1156 5085 ApoI r'AATT_y Cuts at: 1493 3550 3776 3928 6037 1493 Size: 2057 226 152 2109 1697 Fragments arranged by size: 2109 2057 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4795 160 Size: 872 3763 1606 Fragments arranged by size: 3763 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 AvaI C'yCGr_G Cuts at: 1870 1943 2266 3176 3317 3566 1870 Size: 73 323 910 141 249 4545 Fragments arranged by size: 4545 910 323 249 141 73 BamHI G'GATC_C Cuts at: 3571 3938 3571 Size: 367 5874 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2714 3209 3562 5017 618 Size: 325 217 627 927 495 353 1455 1842 Fragments arranged by size: 1842 1455 927 627 495 353 325 217 BanII G_rGCy'C Cuts at: 1391 2187 3152 3176 3196 3235 3560 1391 Size: 796 965 24 20 39 325 4072 Fragments arranged by size: 4072 965 796 325 39 24 20 BbeI G_GCGC'C Cuts at: 3213 3213 Size: 6241 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5270 BbvCI CC'TCA_GC Cuts at: 2546 2546 Size: 6241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2588 2622 3170 3204 4432 Size: 34 50 34 567 34 548 34 1228 Cuts at: 4432 4466 1903 Size: 34 3678 Fragments arranged by size: 3678 1228 567 548 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4133 5343 4133 Size: 1210 5031 BclI T'GATC_A Cuts at: 1548 1548 Size: 6241 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3051 4852 136 Size: 107 122 71 631 605 1379 1801 1525 Fragments arranged by size: 1801 1525 1379 631 605 122 107 71 BglII A'GATC_T Cuts at: 2833 6236 2833 Size: 3403 2838 BlpI GC'TnA_GC Cuts at: 3658 3658 Size: 6241 Bme1580I G_kGCm'C Cuts at: 1790 2575 3152 3235 3269 4302 5458 1790 Size: 785 577 83 34 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 785 577 83 34 BmgBI CAC'GTC Cuts at: 1318 2338 1318 Size: 1020 5221 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3972 4930 442 Size: 416 53 83 399 24 2555 958 1753 Fragments arranged by size: 2555 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6241 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2172 2204 2172 Size: 32 6209 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2142 2353 3009 3269 3277 3470 4901 1239 Size: 903 211 656 260 8 193 1431 2579 Fragments arranged by size: 2579 1431 903 656 260 211 193 8 Bpu10I CC'TnA_GC Cuts at: 2546 2546 Size: 6241 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 2741 4366 5231 5529 2240 Size: 501 1625 865 298 2952 Fragments arranged by size: 2952 1625 865 501 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2160 2469 2780 4904 882 Size: 617 661 309 311 2124 2219 Fragments arranged by size: 2219 2124 661 617 311 309 BsaAI yAC'GTr Cuts at: 493 1204 3184 3264 4032 493 Size: 711 1980 80 768 2702 Fragments arranged by size: 2702 1980 768 711 80 BsaBI GATnn'nnATC Cuts at: 6235 6235 Size: 6241 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2910 3210 4048 4430 Size: 53 83 186 936 1377 300 838 382 Cuts at: 4430 5065 275 Size: 635 1451 Fragments arranged by size: 1451 1377 936 838 635 382 300 186 83 53 BsaWI w'CCGG_w Cuts at: 4674 5072 5346 5493 5904 4674 Size: 398 274 147 411 5011 Fragments arranged by size: 5011 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2095 2125 2742 2772 1178 Size: 30 165 30 692 30 617 30 4647 Fragments arranged by size: 4647 692 617 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2100 2103 2261 2495 2729 2798 3335 3367 Size: 914 3 158 234 234 69 537 32 Cuts at: 3367 1186 Size: 4060 Fragments arranged by size: 4060 914 537 234 234 158 69 32 3 BseYI C'CCAG_C Cuts at: 2231 2330 3151 5444 5940 2231 Size: 99 821 2293 496 2532 Fragments arranged by size: 2532 2293 821 496 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2579 2989 3017 3116 3329 1163 Size: 573 843 410 28 99 213 4075 Fragments arranged by size: 4075 843 573 410 213 99 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3691 4452 5480 1540 Size: 275 186 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2253 2452 3142 3183 3263 3537 4302 4387 5458 Size: 199 690 41 80 274 765 85 1071 Cuts at: 5458 2253 Size: 3036 Fragments arranged by size: 3036 1071 765 690 274 199 85 80 41 BsmI GAATG_Cn' Cuts at: 3752 3845 3752 Size: 93 6148 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2253 2452 2575 3142 3152 3176 Size: 399 397 66 199 123 567 10 24 Cuts at: 3176 3183 3196 3235 3263 3269 3537 3560 4302 Size: 7 13 39 28 6 268 23 742 Cuts at: 4302 4387 5458 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 397 268 199 123 85 66 39 28 24 23 13 10 7 6 BspEI T'CCGG_A Cuts at: 5904 5904 Size: 6241 BspHI T'CATG_A Cuts at: 2145 4130 5860 2145 Size: 1985 1730 2526 Fragments arranged by size: 2526 1985 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2308 3519 3596 844 Size: 1464 1211 77 3489 Fragments arranged by size: 3489 1464 1211 77 BsrBI CCG'CTC Cuts at: 1394 1823 3441 4128 1394 Size: 429 1618 687 3507 Fragments arranged by size: 3507 1618 687 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2878 4736 4910 65 Size: 1183 1630 1858 174 1396 Fragments arranged by size: 1858 1630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4885 5072 1700 Size: 110 3075 187 2869 Fragments arranged by size: 3075 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6241 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6241 BssSI C'ACGA_G Cuts at: 2597 4301 5313 2597 Size: 1704 1012 3525 Fragments arranged by size: 3525 1704 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2323 794 Size: 1529 4712 BstBI TT'CG_AA Cuts at: 5045 5045 Size: 6241 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6241 BstXI CCAn_nnnn'nTGG Cuts at: 2221 2421 3106 3247 2221 Size: 200 685 141 5215 Fragments arranged by size: 5215 685 200 141 BstYI r'GATC_y Cuts at: 1972 2833 3571 3938 4323 4340 5792 5878 5890 Size: 861 738 367 385 17 1452 86 12 Cuts at: 5890 6236 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 861 738 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2293 2741 2293 Size: 448 5793 BtgI C'CryG_G Cuts at: 513 1065 2515 3049 3105 3205 513 Size: 552 1450 534 56 100 3549 Fragments arranged by size: 3549 1450 552 534 100 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2434 2650 487 Size: 683 56 1208 216 4078 Fragments arranged by size: 4078 1208 683 216 56 BtsI GCAGTG_nn' Cuts at: 2328 2495 2619 2891 3757 4551 4571 2328 Size: 167 124 272 866 794 20 3998 Fragments arranged by size: 3998 866 794 272 167 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6241 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6206 DraI TTT'AAA Cuts at: 3545 3545 Size: 6241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5248 809 Size: 507 3932 1802 Fragments arranged by size: 3932 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2011 2518 2802 Size: 54 1425 50 275 18 181 507 284 Cuts at: 2802 2815 3052 3224 3688 4577 6206 8 Size: 13 237 172 464 889 1629 43 Fragments arranged by size: 1629 1425 889 507 464 284 275 237 181 172 54 50 43 18 13 EagI C'GGCC_G Cuts at: 1537 1812 3688 1537 Size: 275 1876 4090 Fragments arranged by size: 4090 1876 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2157 2472 2775 3389 4171 1353 Size: 804 315 303 614 782 3423 Fragments arranged by size: 3423 804 782 614 315 303 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4828 5202 5348 387 Size: 1064 138 3239 374 146 1280 Fragments arranged by size: 3239 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2142 2353 3009 3269 3277 3470 4304 Size: 393 510 211 656 260 8 193 834 Cuts at: 4304 4901 5688 5956 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 834 787 656 597 510 393 268 260 211 193 8 EcoNI CCTnn'n_nnAGG Cuts at: 2597 3239 3394 2597 Size: 642 155 5444 Fragments arranged by size: 5444 642 155 EcoO109I rG'GnC_Cy Cuts at: 2043 2270 2297 2708 3148 3231 3232 3283 2043 Size: 227 27 411 440 83 1 51 5001 Fragments arranged by size: 5001 440 411 227 83 51 27 1 EcoRI G'AATT_C Cuts at: 3550 3928 3550 Size: 378 5863 FspI TGC'GCA Cuts at: 4747 4747 Size: 6241 HaeII r_GCGC'y Cuts at: 1678 3099 3213 3256 3473 5388 1678 Size: 1421 114 43 217 1915 2531 Fragments arranged by size: 2531 1915 1421 217 114 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2190 2222 2262 2294 2753 Size: 32 135 32 618 32 40 32 459 Cuts at: 2753 2785 4882 4914 4956 4988 1373 Size: 32 2097 32 42 32 2626 Fragments arranged by size: 2626 2097 618 459 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3585 3831 669 Size: 988 281 1647 246 3079 Fragments arranged by size: 3079 1647 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6241 HpaI GTT'AAC Cuts at: 3831 3831 Size: 6241 KasI G'GCGC_C Cuts at: 3209 3209 Size: 6241 KpnI G_GTAC'C Cuts at: 3566 3566 Size: 6241 MfeI C'AATT_G Cuts at: 3840 3840 Size: 6241 MluI A'CGCG_T Cuts at: 6117 6117 Size: 6241 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5355 5539 1305 Size: 4050 184 2007 Fragments arranged by size: 4050 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2013 2520 2817 3054 3226 Size: 54 1425 343 181 507 297 237 172 Cuts at: 3226 10 Size: 3025 Fragments arranged by size: 3025 1425 507 343 297 237 181 172 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 4199 4558 4717 518 Size: 648 978 2055 359 159 2042 Fragments arranged by size: 2055 2042 978 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2916 2955 2965 2987 3062 3155 3429 3517 Size: 1310 39 10 22 75 93 274 88 Cuts at: 3517 3657 4334 5482 5727 1606 Size: 140 677 1148 245 2120 Fragments arranged by size: 2120 1310 1148 677 274 245 140 93 88 75 39 22 10 NarI GG'CG_CC Cuts at: 3210 3210 Size: 6241 NcoI C'CATG_G Cuts at: 513 1065 3105 3205 513 Size: 552 2040 100 3549 Fragments arranged by size: 3549 2040 552 100 NdeI CA'TA_TG Cuts at: 387 387 Size: 6241 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6241 NotI GC'GGCC_GC Cuts at: 3688 3688 Size: 6241 NspI r_CATG'y Cuts at: 1132 1229 2143 2504 2801 2859 3599 5144 1132 Size: 97 914 361 297 58 740 1545 2229 Fragments arranged by size: 2229 1545 914 740 361 297 97 58 PasI CC'CwG_GG Cuts at: 1348 2576 1348 Size: 1228 5013 PciI A'CATG_T Cuts at: 5140 5140 Size: 6241 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6241 PmeI GTTT'AAAC Cuts at: 3545 3545 Size: 6241 PmlI CAC'GTG Cuts at: 3184 3264 3184 Size: 80 6161 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4310 4342 5823 5855 4310 Size: 32 1481 32 4696 Fragments arranged by size: 4696 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2270 2297 3283 2270 Size: 27 986 5228 Fragments arranged by size: 5228 986 27 PshAI GACnn'nnGTC Cuts at: 1613 2461 1613 Size: 848 5393 PsiI TTA'TAA Cuts at: 3811 3811 Size: 6241 PspOMI G'GGCC_C Cuts at: 3148 3231 3148 Size: 83 6158 PstI C_TGCA'G Cuts at: 830 2614 2964 3147 3160 3357 3417 3516 3593 Size: 1784 350 183 13 197 60 99 77 Cuts at: 3593 830 Size: 3478 Fragments arranged by size: 3478 1784 350 197 183 99 77 60 13 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6241 PvuII CAG'CTG Cuts at: 2965 2987 3062 3155 3517 2965 Size: 22 75 93 362 5689 Fragments arranged by size: 5689 362 93 75 22 RsrII CG'GwC_CG Cuts at: 2769 2769 Size: 6241 SalI G'TCGA_C Cuts at: 1936 3583 1936 Size: 1647 4594 SanDI GG'GwC_CC Cuts at: 2270 2270 Size: 6241 SbfI CC_TGCA'GG Cuts at: 3593 3593 Size: 6241 ScaI AGT'ACT Cuts at: 2374 4489 6201 2374 Size: 2115 1712 2414 Fragments arranged by size: 2414 2115 1712 SfcI C'TryA_G Cuts at: 826 1045 2610 2960 3143 3156 3298 3353 3413 Size: 219 1565 350 183 13 142 55 60 Cuts at: 3413 3512 3589 4724 5405 5596 826 Size: 99 77 1135 681 191 1471 Fragments arranged by size: 1565 1471 1135 681 350 219 191 183 142 99 77 60 55 13 SfoI GGC'GCC Cuts at: 3211 3211 Size: 6241 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6241 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6241 SmaI CCC'GGG Cuts at: 1872 2268 3568 1872 Size: 396 1300 4545 Fragments arranged by size: 4545 1300 396 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 2720 4345 5246 5508 820 Size: 197 926 312 465 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 465 312 262 197 SnaBI TAC'GTA Cuts at: 493 4032 493 Size: 3539 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6241 SphI G_CATG'C Cuts at: 1132 3599 1132 Size: 2467 3774 SspI AAT'ATT Cuts at: 5 52 4165 5 Size: 47 4113 2081 Fragments arranged by size: 4113 2081 47 StuI AGG'CCT Cuts at: 1517 2603 1517 Size: 1086 5155 StyI C'CwwG_G Cuts at: 513 1065 3105 3205 3240 3390 3680 513 Size: 552 2040 100 35 150 290 3074 Fragments arranged by size: 3074 2040 552 290 150 100 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2757 4268 4285 4438 4623 5068 325 Size: 1255 1177 1511 17 153 185 445 1498 Fragments arranged by size: 1511 1498 1255 1177 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2372 4487 6199 Size: 274 80 33 51 1443 395 2115 1712 Cuts at: 6199 96 Size: 138 Fragments arranged by size: 2115 1712 1443 395 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2742 2774 6201 6233 2742 Size: 32 3427 32 2750 Fragments arranged by size: 3427 2750 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6241 XbaI T'CTAG_A Cuts at: 3577 3577 Size: 6241 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2377 2377 Size: 6241 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6241 XmaI C'CCGG_G Cuts at: 1870 2266 3566 1870 Size: 396 1300 4545 Fragments arranged by size: 4545 1300 396 XmnI GAAnn'nnTTC Cuts at: 4370 4370 Size: 6241 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2911 4049 276 Size: 53 83 186 936 1377 1138 2468 Fragments arranged by size: 2468 1377 1138 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI SacI SacII SapI SexAI SfiI SrfI SwaI