(Linear) (Six-base) MAPSORT of: KIEE4124.seq Check: 3754 from: 1 to: 1536 KIEE4124.orf With 182 enzymes: SgfI * March 22, 2012 17:24 .. AatII G_ACGT'C Cuts at: 0 908 1536 Size: 908 628 AfeI AGC'GCT Cuts at: 0 1249 1466 1536 Size: 1249 217 70 Fragments arranged by size: 1249 217 70 AhdI GACnn_n'nnGTC Cuts at: 0 271 1536 Size: 271 1265 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 604 988 1536 Size: 604 384 548 Fragments arranged by size: 604 548 384 AlwNI CAG_nnn'CTG Cuts at: 0 295 994 1536 Size: 295 699 542 Fragments arranged by size: 699 542 295 ApaI G_GGCC'C Cuts at: 0 1147 1230 1536 Size: 1147 83 306 Fragments arranged by size: 1147 306 83 AvaI C'yCGr_G Cuts at: 0 261 1171 1312 1536 Size: 261 910 141 224 Fragments arranged by size: 910 261 224 141 BanI G'GyrC_C Cuts at: 0 709 1204 1536 Size: 709 495 332 Fragments arranged by size: 709 495 332 BanII G_rGCy'C Cuts at: 0 182 1147 1171 1191 1230 1536 Size: 182 965 24 20 39 306 Fragments arranged by size: 965 306 182 39 24 20 BbeI G_GCGC'C Cuts at: 0 1208 1536 Size: 1208 328 BbvCI CC'TCA_GC Cuts at: 0 541 1536 Size: 541 995 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 583 617 1165 1199 1536 Size: 583 34 548 34 337 Fragments arranged by size: 583 548 337 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1046 1536 Size: 1046 490 BglII A'GATC_T Cuts at: 0 828 1536 Size: 828 708 Bme1580I G_kGCm'C Cuts at: 0 570 1147 1230 1264 1536 Size: 570 577 83 34 272 Fragments arranged by size: 577 570 272 83 34 BmgBI CAC'GTC Cuts at: 0 333 1536 Size: 333 1203 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 167 199 1536 Size: 167 32 1337 Fragments arranged by size: 1337 167 32 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 348 1004 1264 1272 1465 1536 Size: 137 211 656 260 8 193 71 Fragments arranged by size: 656 260 211 193 137 71 8 Bpu10I CC'TnA_GC Cuts at: 0 541 1536 Size: 541 995 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 235 736 1536 Size: 235 501 800 Fragments arranged by size: 800 501 235 BsaI GGTCTCn'nnnn_ Cuts at: 0 155 464 775 1536 Size: 155 309 311 761 Fragments arranged by size: 761 311 309 155 BsaAI yAC'GTr Cuts at: 0 1179 1259 1536 Size: 1179 80 277 Fragments arranged by size: 1179 277 80 BsaHI Gr'CG_yC Cuts at: 0 905 1205 1536 Size: 905 300 331 Fragments arranged by size: 905 331 300 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 90 120 737 767 1536 Size: 90 30 617 30 769 Fragments arranged by size: 769 617 90 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 95 98 256 490 724 793 1330 1362 Size: 95 3 158 234 234 69 537 32 Cuts at: 1362 1536 Size: 174 Fragments arranged by size: 537 234 234 174 158 95 69 32 3 BseYI C'CCAG_C Cuts at: 0 226 325 1146 1536 Size: 226 99 821 390 Fragments arranged by size: 821 390 226 99 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 984 1012 1111 1324 1536 Size: 574 410 28 99 213 212 Fragments arranged by size: 574 410 213 212 99 28 BsiHKAI G_wGCw'C Cuts at: 0 248 447 1137 1178 1258 1532 1536 Size: 248 199 690 41 80 274 4 Fragments arranged by size: 690 274 248 199 80 41 4 Bsp1286I G_dGCh'C Cuts at: 0 182 248 447 570 1137 1147 1171 1178 Size: 182 66 199 123 567 10 24 7 Cuts at: 1178 1191 1230 1258 1264 1532 1536 Size: 13 39 28 6 268 4 Fragments arranged by size: 567 268 199 182 123 66 39 28 24 13 10 7 6 4 BspHI T'CATG_A Cuts at: 0 140 1536 Size: 140 1396 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 303 1514 1536 Size: 303 1211 22 Fragments arranged by size: 1211 303 22 BsrBI CCG'CTC Cuts at: 0 1436 1536 Size: 1436 100 BsrDI GCAATG_nn' Cuts at: 0 873 1536 Size: 873 663 BssSI C'ACGA_G Cuts at: 0 592 1536 Size: 592 944 BstAPI GCAn_nnn'nTGC Cuts at: 0 318 1536 Size: 318 1218 BstXI CCAn_nnnn'nTGG Cuts at: 0 216 416 1101 1242 1536 Size: 216 200 685 141 294 Fragments arranged by size: 685 294 216 200 141 BstYI r'GATC_y Cuts at: 0 828 1536 Size: 828 708 Bsu36I CC'TnA_GG Cuts at: 0 288 736 1536 Size: 288 448 800 Fragments arranged by size: 800 448 288 BtgI C'CryG_G Cuts at: 0 510 1044 1100 1200 1536 Size: 510 534 56 100 336 Fragments arranged by size: 534 510 336 100 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 429 645 1536 Size: 429 216 891 Fragments arranged by size: 891 429 216 BtsI GCAGTG_nn' Cuts at: 0 323 490 614 886 1536 Size: 323 167 124 272 650 Fragments arranged by size: 650 323 272 167 124 EaeI y'GGCC_r Cuts at: 0 6 513 797 810 1047 1219 1536 Size: 6 507 284 13 237 172 317 Fragments arranged by size: 507 317 284 237 172 13 6 EarI CTCTTCn'nnn_ Cuts at: 0 152 467 770 1384 1536 Size: 152 315 303 614 152 Fragments arranged by size: 614 315 303 152 152 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 137 348 1004 1264 1272 1465 1536 Size: 137 211 656 260 8 193 71 Fragments arranged by size: 656 260 211 193 137 71 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 592 1234 1389 1536 Size: 592 642 155 147 Fragments arranged by size: 642 592 155 147 EcoO109I rG'GnC_Cy Cuts at: 0 38 265 292 703 1143 1226 1227 1278 Size: 38 227 27 411 440 83 1 51 Cuts at: 1278 1536 Size: 258 Fragments arranged by size: 440 411 258 227 83 51 38 27 1 HaeII r_GCGC'y Cuts at: 0 1094 1208 1251 1468 1536 Size: 1094 114 43 217 68 Fragments arranged by size: 1094 217 114 68 43 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 185 217 257 289 748 780 1536 Size: 185 32 40 32 459 32 756 Fragments arranged by size: 756 459 185 40 32 32 32 KasI G'GCGC_C Cuts at: 0 1204 1536 Size: 1204 332 MscI TGG'CCA Cuts at: 0 8 515 812 1049 1221 1536 Size: 8 507 297 237 172 315 Fragments arranged by size: 507 315 297 237 172 8 MslI CAynn'nnrTG Cuts at: 0 139 1536 Size: 139 1397 MspA1I CmG'CkG Cuts at: 0 911 950 960 982 1057 1150 1424 1512 Size: 911 39 10 22 75 93 274 88 Cuts at: 1512 1536 Size: 24 Fragments arranged by size: 911 274 93 88 75 39 24 22 10 NarI GG'CG_CC Cuts at: 0 1205 1536 Size: 1205 331 NcoI C'CATG_G Cuts at: 0 1100 1200 1536 Size: 1100 100 336 Fragments arranged by size: 1100 336 100 NspI r_CATG'y Cuts at: 0 138 499 796 854 1536 Size: 138 361 297 58 682 Fragments arranged by size: 682 361 297 138 58 PasI CC'CwG_GG Cuts at: 0 571 1536 Size: 571 965 PmlI CAC'GTG Cuts at: 0 1179 1259 1536 Size: 1179 80 277 Fragments arranged by size: 1179 277 80 PpuMI rG'GwC_Cy Cuts at: 0 265 292 1278 1536 Size: 265 27 986 258 Fragments arranged by size: 986 265 258 27 PshAI GACnn'nnGTC Cuts at: 0 456 1536 Size: 456 1080 PspOMI G'GGCC_C Cuts at: 0 1143 1226 1536 Size: 1143 83 310 Fragments arranged by size: 1143 310 83 PstI C_TGCA'G Cuts at: 0 609 959 1142 1155 1352 1412 1511 1536 Size: 609 350 183 13 197 60 99 25 Fragments arranged by size: 609 350 197 183 99 60 25 13 PvuII CAG'CTG Cuts at: 0 960 982 1057 1150 1512 1536 Size: 960 22 75 93 362 24 Fragments arranged by size: 960 362 93 75 24 22 RsrII CG'GwC_CG Cuts at: 0 764 1536 Size: 764 772 SanDI GG'GwC_CC Cuts at: 0 265 1536 Size: 265 1271 ScaI AGT'ACT Cuts at: 0 369 1536 Size: 369 1167 SfcI C'TryA_G Cuts at: 0 605 955 1138 1151 1293 1348 1408 1507 Size: 605 350 183 13 142 55 60 99 Cuts at: 1507 1536 Size: 29 Fragments arranged by size: 605 350 183 142 99 60 55 29 13 SfoI GGC'GCC Cuts at: 0 1206 1536 Size: 1206 330 SmaI CCC'GGG Cuts at: 0 263 1536 Size: 263 1273 SmlI C'TyrA_G Cuts at: 0 250 715 1536 Size: 250 465 821 Fragments arranged by size: 821 465 250 StuI AGG'CCT Cuts at: 0 598 1536 Size: 598 938 StyI C'CwwG_G Cuts at: 0 1100 1200 1235 1385 1536 Size: 1100 100 35 150 151 Fragments arranged by size: 1100 151 150 100 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 752 1536 Size: 752 784 TatI w'GTAC_w Cuts at: 0 367 1536 Size: 367 1169 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 737 769 1536 Size: 737 32 767 Fragments arranged by size: 767 737 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 372 1536 Size: 372 1164 XmaI C'CCGG_G Cuts at: 0 261 1536 Size: 261 1275 ZraI GAC'GTC Cuts at: 0 906 1536 Size: 906 630 Enzymes that do cut: AatII AfeI AhdI AlfI AlwNI ApaI AvaI BanI BanII BbeI BbvCI BcgI BglI BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I NarI NcoI NspI PasI PmlI PpuMI PshAI PspOMI PstI PvuII RsrII SanDI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AflII AflIII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BlpI BmrI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrFI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PpiI PsiI PspXI PsrI PvuI SacI SacII SalI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI