(Circular) (Six-base) MAPSORT of: pFN21AE0564.seq Check: 3921 from: 1 to: 6049 pFN21AE0564 6049 bp With 182 enzymes: SgfI * October 20, 2009 10:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3159 3859 278 Size: 53 83 186 936 1623 700 2468 Fragments arranged by size: 2468 1623 936 700 186 83 53 AccI GT'mk_AC Cuts at: 1937 3392 1937 Size: 1455 4594 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 3084 4176 4549 1563 Size: 1521 1092 373 3063 Fragments arranged by size: 3063 1521 1092 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2530 2693 2903 4112 5496 5764 1632 Size: 898 163 210 1209 1384 268 1917 Fragments arranged by size: 1917 1384 1209 898 268 210 163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 3186 4948 5925 1203 Size: 1983 1762 977 1327 Fragments arranged by size: 1983 1762 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 2434 2957 4778 2434 Size: 523 1821 3705 Fragments arranged by size: 3705 1821 523 AleI CACnn'nnGTG Cuts at: 1166 3263 1166 Size: 2097 3952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2382 1184 Size: 1198 4851 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2846 2878 2846 Size: 32 6017 AlwNI CAG_nnn'CTG Cuts at: 2214 3148 3792 5364 2214 Size: 934 644 1572 2899 Fragments arranged by size: 2899 1572 934 644 ApaI G_GGCC'C Cuts at: 2252 3166 2252 Size: 914 5135 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 3358 3584 3736 5845 1493 Size: 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 3374 1870 Size: 73 1431 4545 Fragments arranged by size: 4545 1431 73 BamHI G'GATC_C Cuts at: 2385 2616 3379 3746 2385 Size: 231 763 367 4688 Fragments arranged by size: 4688 763 367 231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3370 4825 618 Size: 325 217 627 1583 1455 1842 Fragments arranged by size: 1842 1583 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2252 2463 3166 3368 1391 Size: 861 211 703 202 4072 Fragments arranged by size: 4072 861 703 211 202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5078 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4240 4274 1903 Size: 34 2303 34 3678 Fragments arranged by size: 3678 2303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 3075 1548 Size: 1527 4522 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2531 3098 3339 Size: 107 122 71 631 605 859 567 241 Cuts at: 3339 4660 136 Size: 1321 1525 Fragments arranged by size: 1525 1321 859 631 605 567 241 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 2252 3166 3270 3285 4110 5266 1790 Size: 462 914 104 15 825 1156 2573 Fragments arranged by size: 2573 1156 914 825 462 104 15 BmgBI CAC'GTC Cuts at: 1318 2851 1318 Size: 1533 4516 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3780 4738 442 Size: 416 53 83 399 24 2363 958 1753 Fragments arranged by size: 2363 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 2396 2447 2672 2819 2990 3140 3191 Size: 1136 21 51 225 147 171 150 51 Cuts at: 3191 3224 4709 1239 Size: 33 1485 2579 Fragments arranged by size: 2579 1485 1136 225 171 150 147 51 51 33 21 Bpu10I CC'TnA_GC Cuts at: 2103 2103 Size: 6049 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2033 4174 5039 5337 2033 Size: 2141 865 298 2745 Fragments arranged by size: 2745 2141 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4712 882 Size: 617 3213 2219 Fragments arranged by size: 3213 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3189 3840 493 Size: 711 1985 651 2702 Fragments arranged by size: 2702 1985 711 651 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3156 3856 4238 4873 Size: 53 83 186 936 1623 700 382 635 Cuts at: 4873 275 Size: 1451 Fragments arranged by size: 1623 1451 936 700 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3192 4482 4880 5154 5301 5712 3192 Size: 1290 398 274 147 411 3529 Fragments arranged by size: 3529 1290 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2407 2437 2672 2702 2795 Size: 30 165 30 1004 30 235 30 93 Cuts at: 2795 2825 2846 2876 3224 3254 1178 Size: 30 21 30 348 30 3973 Fragments arranged by size: 3973 1004 348 235 165 93 30 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2471 2693 2723 2816 2894 1186 Size: 1285 222 30 93 78 4341 Fragments arranged by size: 4341 1285 222 93 78 30 BseYI C'CCAG_C Cuts at: 2988 3051 3093 5252 5748 2988 Size: 63 42 2159 496 3289 Fragments arranged by size: 3289 2159 496 63 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2754 3238 1163 Size: 573 1018 484 3974 Fragments arranged by size: 3974 1018 573 484 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2320 3499 4260 5288 1540 Size: 275 186 319 1179 761 1028 2301 Fragments arranged by size: 2301 1179 1028 761 319 275 186 BsiHKAI G_wGCw'C Cuts at: 2415 2463 4110 4195 5266 2415 Size: 48 1647 85 1071 3198 Fragments arranged by size: 3198 1647 1071 85 48 BsmI GAATG_Cn' Cuts at: 2371 2500 3560 3653 2371 Size: 129 1060 93 4767 Fragments arranged by size: 4767 1060 129 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2944 2944 Size: 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2252 2415 2463 3166 3270 3285 3368 Size: 399 462 163 48 703 104 15 83 Cuts at: 3368 4110 4195 5266 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 462 399 163 104 85 83 48 15 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3404 844 Size: 2560 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3124 3936 1394 Size: 429 1301 812 3507 Fragments arranged by size: 3507 1301 812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2293 4544 4718 65 Size: 1183 1045 2251 174 1396 Fragments arranged by size: 2251 1396 1183 1045 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 4693 4880 1700 Size: 110 287 2596 187 2869 Fragments arranged by size: 2869 2596 287 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 2495 3321 794 Size: 1701 826 3522 Fragments arranged by size: 3522 1701 826 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2797 Size: 6049 BstYI r'GATC_y Cuts at: 1972 2385 2616 3105 3379 3746 4131 4148 5600 Size: 413 231 489 274 367 385 17 1452 Cuts at: 5600 5686 5698 6044 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 489 413 385 367 346 274 231 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2999 2999 Size: 6049 BtgI C'CryG_G Cuts at: 513 1065 2133 2244 2826 513 Size: 552 1068 111 582 3736 Fragments arranged by size: 3736 1068 582 552 111 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 3074 3565 4359 4379 3074 Size: 491 794 20 4744 Fragments arranged by size: 4744 794 491 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2637 2637 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2174 2325 2571 Size: 54 1425 50 275 18 344 151 246 Cuts at: 2571 2898 3496 4385 6014 8 Size: 327 598 889 1629 43 Fragments arranged by size: 1629 1425 889 598 344 327 275 246 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3979 1353 Size: 2626 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2461 2461 Size: 6049 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2375 2396 2447 2530 2672 2693 2819 Size: 393 743 21 51 83 142 21 126 Cuts at: 2819 2903 2990 3140 3191 3224 4112 4709 5496 Size: 84 87 150 51 33 888 597 787 Cuts at: 5496 5764 1239 Size: 268 1524 Fragments arranged by size: 1524 888 787 743 597 393 268 150 142 126 87 84 83 51 51 33 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 2675 2675 Size: 6049 EcoO109I rG'GnC_Cy Cuts at: 2249 2274 2249 Size: 25 6024 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 2612 4555 2612 Size: 1943 4106 HaeII r_GCGC'y Cuts at: 1678 5196 1678 Size: 3518 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2420 2452 2797 2829 2943 Size: 32 135 32 848 32 345 32 114 Cuts at: 2943 2975 4690 4722 4764 4796 1373 Size: 32 1715 32 42 32 2626 Fragments arranged by size: 2626 1715 848 345 135 114 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2962 3393 3639 669 Size: 988 281 1024 431 246 3079 Fragments arranged by size: 3079 1024 988 431 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5163 5347 1305 Size: 3858 184 2007 Fragments arranged by size: 3858 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 2573 2900 10 Size: 54 1425 343 495 246 327 3159 Fragments arranged by size: 3159 1425 495 343 327 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2759 3263 4007 4366 4525 518 Size: 648 1593 504 744 359 159 2042 Fragments arranged by size: 2042 1593 744 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2184 2259 2353 2584 3055 3097 3465 Size: 479 99 75 94 231 471 42 368 Cuts at: 3465 4142 5290 5535 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 479 471 368 245 231 99 94 75 42 NaeI GCC'GGC Cuts at: 2099 2099 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 2133 2244 2826 513 Size: 552 1068 111 582 3736 Fragments arranged by size: 3736 1068 582 552 111 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NgoMIV G'CCGG_C Cuts at: 2097 2097 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2142 3319 3407 4952 1132 Size: 97 913 1177 88 1545 2229 Fragments arranged by size: 2229 1545 1177 913 97 88 PasI CC'CwG_GG Cuts at: 1348 3337 1348 Size: 1989 4060 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2134 2896 1108 Size: 1026 762 4261 Fragments arranged by size: 4261 1026 762 PfoI T'CCnGG_A Cuts at: 2619 2619 Size: 6049 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2846 2878 4118 4150 5631 5663 2846 Size: 32 1240 32 1481 32 3232 Fragments arranged by size: 3232 1481 1240 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspOMI G'GGCC_C Cuts at: 2248 3162 2248 Size: 914 5135 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3122 3154 3122 Size: 32 6017 PstI C_TGCA'G Cuts at: 830 2985 3401 830 Size: 2155 416 3478 Fragments arranged by size: 3478 2155 416 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2184 2259 2353 2584 3055 3097 2184 Size: 75 94 231 471 42 5136 Fragments arranged by size: 5136 471 231 94 75 42 SacI G_AGCT'C Cuts at: 2463 2463 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SexAI A'CCwGG_T Cuts at: 3141 3141 Size: 6049 SfcI C'TryA_G Cuts at: 826 1045 2981 3043 3397 4532 5213 5404 826 Size: 219 1936 62 354 1135 681 191 1471 Fragments arranged by size: 1936 1471 1135 681 354 219 191 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2048 4153 5054 5316 820 Size: 197 926 105 2105 901 262 1553 Fragments arranged by size: 2105 1553 926 901 262 197 105 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 2142 3319 3407 1132 Size: 1010 1177 88 3774 Fragments arranged by size: 3774 1177 1010 88 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 2396 1517 Size: 879 5170 StyI C'CwwG_G Cuts at: 513 1065 2133 2244 2484 2826 3488 513 Size: 552 1068 111 240 342 662 3074 Fragments arranged by size: 3074 1068 662 552 342 240 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2062 2140 4076 4093 4246 4431 4876 Size: 1255 482 78 1936 17 153 185 445 Cuts at: 4876 325 Size: 1498 Fragments arranged by size: 1936 1498 1255 482 445 185 153 78 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4295 6007 96 Size: 274 80 33 51 1443 2318 1712 138 Fragments arranged by size: 2318 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2407 2439 2672 2704 2795 2827 6009 6041 2407 Size: 32 233 32 91 32 3182 32 2415 Fragments arranged by size: 3182 2415 233 91 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2225 2581 2225 Size: 356 5693 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3157 3857 276 Size: 53 83 186 936 1623 700 2468 Fragments arranged by size: 2468 1623 936 700 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI