Restriction map for
pFN21AE0564
(Linear) (Six-base) MAPSORT of: KIEE0564.seq Check: 85 from: 1 to: 1344
KIEE0564.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 0 1154 1344
Size: 1154 190
AclI AA'CG_TT
Cuts at: 0 1079 1344
Size: 1079 265
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 688 898 1344
Size: 525 163 210 446
Fragments arranged by size:
525 446 210 163
AflIII A'CryG_T
Cuts at: 0 1181 1344
Size: 1181 163
AhdI GACnn_n'nnGTC
Cuts at: 0 429 952 1344
Size: 429 523 392
Fragments arranged by size:
523 429 392
AleI CACnn'nnGTG
Cuts at: 0 1258 1344
Size: 1258 86
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 377 1344
Size: 377 967
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 841 873 1344
Size: 841 32 471
Fragments arranged by size:
841 471 32
AlwNI CAG_nnn'CTG
Cuts at: 0 209 1143 1344
Size: 209 934 201
Fragments arranged by size:
934 209 201
ApaI G_GGCC'C
Cuts at: 0 247 1161 1344
Size: 247 914 183
Fragments arranged by size:
914 247 183
BamHI G'GATC_C
Cuts at: 0 380 611 1344
Size: 380 231 733
Fragments arranged by size:
733 380 231
BanII G_rGCy'C
Cuts at: 0 247 458 1161 1344
Size: 247 211 703 183
Fragments arranged by size:
703 247 211 183
BclI T'GATC_A
Cuts at: 0 1070 1344
Size: 1070 274
BglI GCCn_nnn'nGGC
Cuts at: 0 526 1093 1334 1344
Size: 526 567 241 10
Fragments arranged by size:
567 526 241 10
Bme1580I G_kGCm'C
Cuts at: 0 247 1161 1265 1280 1344
Size: 247 914 104 15 64
Fragments arranged by size:
914 247 104 64 15
BmgBI CAC'GTC
Cuts at: 0 846 1344
Size: 846 498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 391 442 667 814 985 1135 1186
Size: 370 21 51 225 147 171 150 51
Cuts at: 1186 1219 1344
Size: 33 125
Fragments arranged by size:
370 225 171 150 147 125 51 51
33 21
Bpu10I CC'TnA_GC
Cuts at: 0 98 1344
Size: 98 1246
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 1344
Size: 28 1316
BsaAI yAC'GTr
Cuts at: 0 1184 1344
Size: 1184 160
BsaHI Gr'CG_yC
Cuts at: 0 1151 1344
Size: 1151 193
BsaWI w'CCGG_w
Cuts at: 0 1187 1344
Size: 1187 157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 402 432 667 697 790 820 841 871
Size: 402 30 235 30 93 30 21 30
Cuts at: 871 1219 1249 1344
Size: 348 30 95
Fragments arranged by size:
402 348 235 95 93 30 30 30
30 30 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 466 688 718 811 889 1344
Size: 466 222 30 93 78 455
Fragments arranged by size:
466 455 222 93 78 30
BseYI C'CCAG_C
Cuts at: 0 983 1046 1088 1344
Size: 983 63 42 256
Fragments arranged by size:
983 256 63 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 1233 1344
Size: 749 484 111
Fragments arranged by size:
749 484 111
BsiEI CG_ry'CG
Cuts at: 0 315 1344
Size: 315 1029
BsiHKAI G_wGCw'C
Cuts at: 0 410 458 1344
Size: 410 48 886
Fragments arranged by size:
886 410 48
BsmI GAATG_Cn'
Cuts at: 0 366 495 1344
Size: 366 129 849
Fragments arranged by size:
849 366 129
BsmBI CGTCTCn'nnnn_
Cuts at: 0 939 1344
Size: 939 405
Bsp1286I G_dGCh'C
Cuts at: 0 247 410 458 1161 1265 1280 1344
Size: 247 163 48 703 104 15 64
Fragments arranged by size:
703 247 163 104 64 48 15
BsrBI CCG'CTC
Cuts at: 0 1119 1344
Size: 1119 225
BsrDI GCAATG_nn'
Cuts at: 0 288 1344
Size: 288 1056
BsrFI r'CCGG_y
Cuts at: 0 92 1344
Size: 92 1252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 490 1316 1344
Size: 490 826 28
Fragments arranged by size:
826 490 28
BstXI CCAn_nnnn'nTGG
Cuts at: 0 792 1344
Size: 792 552
BstYI r'GATC_y
Cuts at: 0 380 611 1100 1344
Size: 380 231 489 244
Fragments arranged by size:
489 380 244 231
Bsu36I CC'TnA_GG
Cuts at: 0 994 1344
Size: 994 350
BtgI C'CryG_G
Cuts at: 0 128 239 821 1344
Size: 128 111 582 523
Fragments arranged by size:
582 523 128 111
BtsI GCAGTG_nn'
Cuts at: 0 1069 1344
Size: 1069 275
DraIII CAC_nnn'GTG
Cuts at: 0 632 1344
Size: 632 712
EaeI y'GGCC_r
Cuts at: 0 169 320 566 893 1344
Size: 169 151 246 327 451
Fragments arranged by size:
451 327 246 169 151
EcoICRI GAG'CTC
Cuts at: 0 456 1344
Size: 456 888
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 370 391 442 525 667 688 814 898
Size: 370 21 51 83 142 21 126 84
Cuts at: 898 985 1135 1186 1219 1344
Size: 87 150 51 33 125
Fragments arranged by size:
370 150 142 126 125 87 84 83
51 51 33 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 670 1344
Size: 670 674
EcoO109I rG'GnC_Cy
Cuts at: 0 244 269 1344
Size: 244 25 1075
Fragments arranged by size:
1075 244 25
FspI TGC'GCA
Cuts at: 0 607 1344
Size: 607 737
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 792 824 938 970 1344
Size: 415 32 345 32 114 32 374
Fragments arranged by size:
415 374 345 114 32 32 32
HincII GTy'rAC
Cuts at: 0 957 1344
Size: 957 387
MscI TGG'CCA
Cuts at: 0 322 568 895 1344
Size: 322 246 327 449
Fragments arranged by size:
449 327 322 246
MslI CAynn'nnrTG
Cuts at: 0 754 1258 1344
Size: 754 504 86
Fragments arranged by size:
754 504 86
MspA1I CmG'CkG
Cuts at: 0 80 179 254 348 579 1050 1092 1344
Size: 80 99 75 94 231 471 42 252
Fragments arranged by size:
471 252 231 99 94 80 75 42
NaeI GCC'GGC
Cuts at: 0 94 1344
Size: 94 1250
NcoI C'CATG_G
Cuts at: 0 128 239 821 1344
Size: 128 111 582 523
Fragments arranged by size:
582 523 128 111
NgoMIV G'CCGG_C
Cuts at: 0 92 1344
Size: 92 1252
NspI r_CATG'y
Cuts at: 0 137 1314 1344
Size: 137 1177 30
Fragments arranged by size:
1177 137 30
PasI CC'CwG_GG
Cuts at: 0 1332 1344
Size: 1332 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 129 891 1344
Size: 129 762 453
Fragments arranged by size:
762 453 129
PfoI T'CCnGG_A
Cuts at: 0 614 1344
Size: 614 730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 1344
Size: 841 32 471
Fragments arranged by size:
841 471 32
PspOMI G'GGCC_C
Cuts at: 0 243 1157 1344
Size: 243 914 187
Fragments arranged by size:
914 243 187
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1117 1149 1344
Size: 1117 32 195
Fragments arranged by size:
1117 195 32
PstI C_TGCA'G
Cuts at: 0 980 1344
Size: 980 364
PvuII CAG'CTG
Cuts at: 0 179 254 348 579 1050 1092 1344
Size: 179 75 94 231 471 42 252
Fragments arranged by size:
471 252 231 179 94 75 42
SacI G_AGCT'C
Cuts at: 0 458 1344
Size: 458 886
SexAI A'CCwGG_T
Cuts at: 0 1136 1344
Size: 1136 208
SfcI C'TryA_G
Cuts at: 0 976 1038 1344
Size: 976 62 306
Fragments arranged by size:
976 306 62
SmlI C'TyrA_G
Cuts at: 0 43 1344
Size: 43 1301
SphI G_CATG'C
Cuts at: 0 137 1314 1344
Size: 137 1177 30
Fragments arranged by size:
1177 137 30
StuI AGG'CCT
Cuts at: 0 391 1344
Size: 391 953
StyI C'CwwG_G
Cuts at: 0 128 239 479 821 1344
Size: 128 111 240 342 523
Fragments arranged by size:
523 342 240 128 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 57 135 1344
Size: 57 78 1209
Fragments arranged by size:
1209 78 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 402 434 667 699 790 822 1344
Size: 402 32 233 32 91 32 522
Fragments arranged by size:
522 402 233 91 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 220 576 1344
Size: 220 356 768
Fragments arranged by size:
768 356 220
ZraI GAC'GTC
Cuts at: 0 1152 1344
Size: 1152 192
Enzymes that do cut:
AatII AclI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI BamHI BanII BclI BglI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BsrBI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EcoICRI Eco57MI EcoNI EcoO109I FspI Hin4I
HincII MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpiI PspOMI PsrI PstI PvuII
SacI SexAI SfcI SmlI SphI StuI StyI TaqII
TstI XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AfeI AflII AgeI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BciVI BfrBI BglII BlpI BmrI
BmtI BplI BsaI BsaBI BsiWI BspEI BspHI BspMI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EarI EciI EcoRI EcoRV
FalI FseI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpuMI PshAI PsiI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI
XmnI