################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccI AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI AspEI AspI AvrII BbeI BbrPI BcgI BclI BfrI BglII BlnI BsaBI BsaI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FseI HindIII HpaI KasI KspI MamI MluI MseI NaeI NarI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 716 GGTACC G^GTACC AccIII 37 TCCGGA T^CCGGA AcsI 844 GAATTC [GA]^AATT[CT] AcyI 1496 GGCGTC G[GA]^CG[CT]C ApoI 844 GAATTC [GA]^AATT[CT] Asp700I 1842 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 716 GGTACC G^GTACC AvaI 649 CCCGAG C^[CT]CG[GA]G BamHI 1738 GGATCC G^GATCC BanI 716 GGTACC G^G[CT][GA]CC BbsI 400 GAAGAC GAAGAC(2/6) BciVI 732 GTATCC GTATCC(6/5) BglI 10 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 549 GCTGAGC GC^T[ACGT]AGC Bpu1102I 549 GCTGAGC GC^T[ACGT]AGC BpuAI 400 GAAGAC GAAGAC(2/6) BsaAI 615 CACGTA [CT]AC^GT[GA] BsaHI 1496 GGCGTC G[GA]^CG[CT]C BsaWI 37 TCCGGA [AT]^CCGG[AT] BseAI 37 TCCGGA T^CCGGA BsgI 940 CTGCAC GTGCAG(16/14) BsmBI 1498 CGTCTC CGTCTC(1/5) BsoBI 649 CCCGAG C^[CT]CG[GA]G BspEI 37 TCCGGA T^CCGGA BspHI 1073 TCATGA T^CATGA BsrFI 269 ACCGGC [GA]^CCGG[CT] BssSI 628 CACGAG CACGAG(-5/-1) BstAPI 942 GCACGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 549 GCTGAGC GC^T[ACGT]AGC Cfr10I 269 ACCGGC [GA]^CCGG[CT] DrdI 1564 GACCCTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1115 CCATGG C^C[GA][CT]GG EarI 1207 CTCTTC CTCTTC(1/4) EcoRI 844 GAATTC G^AATTC EcoT14I 1115 CCATGG C^C[AT][AT]GG Hin1I 1496 GGCGTC G[GA]^CG[CT]C HincII 1453 GTTGAC GT[CT]^[GA]AC HindII 1453 GTTGAC GT[CT]^[GA]AC KpnI 716 GGTACC GGTAC^C Ksp632I 1207 CTCTTC CTCTTC(1/4) MfeI 355 CAATTG C^AATTG MroI 37 TCCGGA T^CCGGA MunI 355 CAATTG C^AATTG NcoI 1115 CCATGG C^CATGG NotI 76 GCGGCCGC GC^GGCCGC PstI 2011 CTGCAG CTGCA^G RcaI 1073 TCATGA T^CATGA SacI 62 GAGCTC GAGCT^C SphI 1274 GCATGC GCATG^C StyI 1115 CCATGG C^C[AT][AT]GG TfiI 1672 GATTC G^A[AT]TC XcmI 1718 CCACTGGTGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1842 GAACCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwNI 1193 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 1358 CAGACACTG AocI 832 CCTGAGG CC^T[ACGT]AGG 913 CCTGAGG ApaI 815 GGGCCC GGGCC^C 890 GGGCCC AspHI 62 GAGCTC G[AT]GC[AT]^C 1882 GAGCAC AviII 878 TGCGCA TGC^GCA 1901 TGCGCA BsiEI 77 CGGCCG CG[GA][CT]^CG 1107 CGGCCG BsiHKAI 62 GAGCTC G[AT]GC[AT]^C 1882 GAGCAC BsmAI 1506 GAGAC GTCTC(1/5) 1499 GTCTC BsrBI 153 CCGCTC CCGCTC(-3/-3) 757 GAGCGG BsrDI 392 CATTGC GCAATG(2/0) 1904 GCAATG Bsu36I 832 CCTGAGG CC^T[ACGT]AGG 913 CCTGAGG EagI 77 CGGCCG C^GGCCG 1107 CGGCCG EclXI 77 CGGCCG C^GGCCG 1107 CGGCCG Eco52I 77 CGGCCG C^GGCCG 1107 CGGCCG Eco81I 832 CCTGAGG CC^T[ACGT]AGG 913 CCTGAGG FspI 878 TGCGCA TGC^GCA 1901 TGCGCA HgaI 1497 GCGTC GACGC(5/10) 1973 GCGTC MslI 510 CACTCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 676 CATGGCGGTG NspI 1274 GCATGC [GA]CATG^[CT] 1406 ACATGC PleI 2029 GACTC GAGTC(4/5) 116 GAGTC PvuII 1576 CAGCTG CAG^CTG 1589 CAGCTG StuI 1414 AGGCCT AGG^CCT 1709 AGGCCT TaqI 188 TCGA T^CGA 1259 TCGA Tsp45I 1004 GTGAC ^GT[GC]AC 1285 GTCAC Tsp509I 356 AATT ^AATT 845 AATT TthHB8I 188 TCGA T^CGA 1259 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AvaII BalI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI TspRI XhoII