(Circular) (Six-base) MAPSORT of: pFN21AE4191.seq Check: 4842 from: 1 to: 6769 pFN21AE4191 6769 bp With 182 enzymes: SgfI * July 6, 2012 17:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4579 278 Size: 53 83 186 936 3043 2468 Fragments arranged by size: 3043 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4112 1937 Size: 2175 4594 Acc65I G'GTAC_C Cuts at: 2721 4090 2721 Size: 1369 5400 AclI AA'CG_TT Cuts at: 1563 4896 5269 1563 Size: 3333 373 3063 Fragments arranged by size: 3333 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4832 6216 6484 1632 Size: 3200 1384 268 1917 Fragments arranged by size: 3200 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6572 AflIII A'CryG_T Cuts at: 1203 2196 2448 2619 2733 5668 6645 1203 Size: 993 252 171 114 2935 977 1327 Fragments arranged by size: 2935 1327 993 977 252 171 114 AgeI A'CCGG_T Cuts at: 5600 5600 Size: 6769 AhdI GACnn_n'nnGTC Cuts at: 5498 5498 Size: 6769 AleI CACnn'nnGTG Cuts at: 1166 2519 1166 Size: 1353 5416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6769 AlwNI CAG_nnn'CTG Cuts at: 3203 3368 4512 6084 3203 Size: 165 1144 1572 3888 Fragments arranged by size: 3888 1572 1144 165 ApaI G_GGCC'C Cuts at: 2824 2899 2824 Size: 75 6694 ApaLI G'TGCA_C Cuts at: 4826 5982 4826 Size: 1156 5613 ApoI r'AATT_y Cuts at: 1493 2849 4078 4304 4456 6565 1493 Size: 1356 1229 226 152 2109 1697 Fragments arranged by size: 2109 1697 1356 1229 226 152 AseI AT'TA_AT Cuts at: 160 1032 5323 160 Size: 872 4291 1606 Fragments arranged by size: 4291 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6769 AvaI C'yCGr_G Cuts at: 1870 1943 2654 4094 1870 Size: 73 711 1440 4545 Fragments arranged by size: 4545 1440 711 73 BamHI G'GATC_C Cuts at: 3743 4099 4466 3743 Size: 356 367 6046 Fragments arranged by size: 6046 367 356 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2721 4090 5545 618 Size: 325 217 627 934 1369 1455 1842 Fragments arranged by size: 1842 1455 1369 934 627 325 217 BanII G_rGCy'C Cuts at: 1391 2071 2697 2824 2837 2899 3560 4000 4088 Size: 680 626 127 13 62 661 440 88 Cuts at: 4088 1391 Size: 4072 Fragments arranged by size: 4072 680 661 626 440 127 88 62 13 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2412 928 Size: 971 513 5285 Fragments arranged by size: 5285 971 513 BbvCI CC'TCA_GC Cuts at: 2088 2321 3416 4050 2088 Size: 233 1095 634 4807 Fragments arranged by size: 4807 1095 634 233 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4960 4994 1903 Size: 34 3023 34 3678 Fragments arranged by size: 3678 3023 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2748 4661 5871 2748 Size: 1913 1210 3646 Fragments arranged by size: 3646 1913 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6769 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 5380 136 Size: 107 122 71 631 605 349 3359 1525 Fragments arranged by size: 3359 1525 631 605 349 122 107 71 BglII A'GATC_T Cuts at: 6764 6764 Size: 6769 BlpI GC'TnA_GC Cuts at: 2555 4186 2555 Size: 1631 5138 Bme1580I G_kGCm'C Cuts at: 1790 2824 2899 4830 5986 1790 Size: 1034 75 1931 1156 2573 Fragments arranged by size: 2573 1931 1156 1034 75 BmgBI CAC'GTC Cuts at: 1318 2197 2449 1318 Size: 879 252 5638 Fragments arranged by size: 5638 879 252 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 2419 4500 Size: 416 53 83 399 24 906 96 2081 Cuts at: 4500 5458 442 Size: 958 1753 Fragments arranged by size: 2081 1753 958 906 416 399 96 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6769 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 3588 3784 2176 Size: 1412 196 5161 Fragments arranged by size: 5161 1412 196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3518 3544 3905 5429 1239 Size: 2279 26 361 1524 2579 Fragments arranged by size: 2579 2279 1524 361 26 Bpu10I CC'TnA_GC Cuts at: 2070 2088 2321 2600 3416 4050 2070 Size: 18 233 279 816 634 4789 Fragments arranged by size: 4789 816 634 279 233 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2792 2855 3230 3305 4894 5759 6057 2792 Size: 63 375 75 1589 865 298 3504 Fragments arranged by size: 3504 1589 865 375 298 75 63 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5432 882 Size: 617 3933 2219 Fragments arranged by size: 3933 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2622 4560 493 Size: 711 1418 1938 2702 Fragments arranged by size: 2702 1938 1418 711 BsaBI GATnn'nnATC Cuts at: 6763 6763 Size: 6769 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3502 4576 4958 5593 Size: 53 83 186 936 1969 1074 382 635 Cuts at: 5593 275 Size: 1451 Fragments arranged by size: 1969 1451 1074 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2042 5202 5600 5874 6021 6432 2042 Size: 3160 398 274 147 411 2379 Fragments arranged by size: 3160 2379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3542 3572 1178 Size: 30 165 30 2139 30 4375 Fragments arranged by size: 4375 2139 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2108 2111 2672 3447 3649 4055 1186 Size: 919 3 3 561 775 202 406 3900 Fragments arranged by size: 3900 919 775 561 406 202 3 3 BseYI C'CCAG_C Cuts at: 2823 3350 3592 5972 6468 2823 Size: 527 242 2380 496 3124 Fragments arranged by size: 3124 2380 527 496 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2930 1163 Size: 573 1194 5002 Fragments arranged by size: 5002 1194 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2085 3115 4219 4980 6008 1540 Size: 275 186 84 1030 1104 761 1028 2301 Fragments arranged by size: 2301 1104 1030 1028 761 275 186 84 BsiHKAI G_wGCw'C Cuts at: 2071 3891 4830 4915 5986 2071 Size: 1820 939 85 1071 2854 Fragments arranged by size: 2854 1820 1071 939 85 BsmI GAATG_Cn' Cuts at: 4280 4373 4280 Size: 93 6676 BsmBI CGTCTCn'nnnn_ Cuts at: 3509 3509 Size: 6769 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2697 2824 2837 2899 3560 3891 Size: 399 281 626 127 13 62 661 331 Cuts at: 3891 4000 4088 4830 4915 5986 1391 Size: 109 88 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 661 626 399 331 281 127 109 88 85 62 13 BspEI T'CCGG_A Cuts at: 2042 6432 2042 Size: 4390 2379 BspHI T'CATG_A Cuts at: 3078 4658 6388 3078 Size: 1580 1730 3459 Fragments arranged by size: 3459 1730 1580 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2364 2733 2961 3195 4023 4124 844 Size: 1520 369 228 234 828 101 3489 Fragments arranged by size: 3489 1520 828 369 234 228 101 BsrBI CCG'CTC Cuts at: 1394 1823 2160 2764 4656 1394 Size: 429 337 604 1892 3507 Fragments arranged by size: 3507 1892 604 429 337 BsrDI GCAATG_nn' Cuts at: 65 1248 2396 3916 5264 5438 65 Size: 1183 1148 1520 1348 174 1396 Fragments arranged by size: 1520 1396 1348 1183 1148 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2274 5413 5600 1700 Size: 110 464 3139 187 2869 Fragments arranged by size: 3139 2869 464 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6769 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6769 BssSI C'ACGA_G Cuts at: 2633 4829 5841 2633 Size: 2196 1012 3561 Fragments arranged by size: 3561 2196 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2953 794 Size: 2159 4610 BstBI TT'CG_AA Cuts at: 5573 5573 Size: 6769 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6769 BstXI CCAn_nnnn'nTGG Cuts at: 3358 3836 3883 3358 Size: 478 47 6244 Fragments arranged by size: 6244 478 47 BstYI r'GATC_y Cuts at: 1972 2173 2673 3743 4099 4466 4851 4868 6320 Size: 201 500 1070 356 367 385 17 1452 Cuts at: 6320 6406 6418 6764 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1070 500 385 367 356 346 201 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2838 2919 2838 Size: 81 6688 BtgI C'CryG_G Cuts at: 513 1065 2004 3120 513 Size: 552 939 1116 4162 Fragments arranged by size: 4162 1116 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2619 487 Size: 683 56 1393 4637 Fragments arranged by size: 4637 1393 683 56 BtsI GCAGTG_nn' Cuts at: 2337 3650 4285 5079 5099 2337 Size: 1313 635 794 20 4007 Fragments arranged by size: 4007 1313 794 635 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6769 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3115 3150 529 Size: 35 2551 35 4148 Fragments arranged by size: 4148 2551 35 35 DraI TTT'AAA Cuts at: 4073 4073 Size: 6769 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3575 5776 809 Size: 507 2259 2201 1802 Fragments arranged by size: 2259 2201 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2082 3090 3112 Size: 54 1425 50 275 18 252 1008 22 Cuts at: 3112 3123 3379 3468 3838 3873 3933 4216 5105 Size: 11 256 89 370 35 60 283 889 Cuts at: 5105 6734 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1008 889 370 283 275 256 252 89 60 54 50 43 35 22 18 11 EagI C'GGCC_G Cuts at: 1537 1812 2082 3112 4216 1537 Size: 275 270 1030 1104 4090 Fragments arranged by size: 4090 1104 1030 275 270 EarI CTCTTCn'nnn_ Cuts at: 1353 3218 4699 1353 Size: 1865 1481 3423 Fragments arranged by size: 3423 1865 1481 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5356 5730 5876 387 Size: 1064 138 3767 374 146 1280 Fragments arranged by size: 3767 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2069 2069 Size: 6769 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3518 3544 3905 4832 5429 6216 6484 Size: 393 1886 26 361 927 597 787 268 Cuts at: 6484 1239 Size: 1524 Fragments arranged by size: 1886 1524 927 787 597 393 361 268 26 EcoNI CCTnn'n_nnAGG Cuts at: 2516 3191 3512 2516 Size: 675 321 5773 Fragments arranged by size: 5773 675 321 EcoO109I rG'GnC_Cy Cuts at: 2022 2317 2842 2915 3485 3568 2022 Size: 295 525 73 570 83 5223 Fragments arranged by size: 5223 570 525 295 83 73 EcoRI G'AATT_C Cuts at: 2849 4078 4456 2849 Size: 1229 378 5162 Fragments arranged by size: 5162 1229 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 6769 FspI TGC'GCA Cuts at: 2885 3908 5275 2885 Size: 1023 1367 4379 Fragments arranged by size: 4379 1367 1023 HaeII r_GCGC'y Cuts at: 1678 2051 2945 3204 5916 1678 Size: 373 894 259 2712 2531 Fragments arranged by size: 2712 2531 894 373 259 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2453 2485 3002 3034 3668 Size: 32 135 32 881 32 517 32 634 Cuts at: 3668 3700 5410 5442 5484 5516 1373 Size: 32 1710 32 42 32 2626 Fragments arranged by size: 2626 1710 881 634 517 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3460 4113 4359 669 Size: 988 281 1522 653 246 3079 Fragments arranged by size: 3079 1522 988 653 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6769 HpaI GTT'AAC Cuts at: 4359 4359 Size: 6769 KpnI G_GTAC'C Cuts at: 2725 4094 2725 Size: 1369 5400 MfeI C'AATT_G Cuts at: 2360 4368 2360 Size: 2008 4761 MluI A'CGCG_T Cuts at: 6645 6645 Size: 6769 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 2569 3322 5883 6067 1305 Size: 1164 100 753 2561 184 2007 Fragments arranged by size: 2561 2007 1164 753 184 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 3125 3381 3470 3875 3935 Size: 54 1425 343 1293 256 89 405 60 Cuts at: 3935 10 Size: 2844 Fragments arranged by size: 2844 1425 1293 405 343 256 89 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 2685 4727 5086 5245 518 Size: 648 1353 166 2042 359 159 2042 Fragments arranged by size: 2042 2042 1353 648 359 166 159 MspA1I CmG'CkG Cuts at: 1606 2087 3228 3583 3596 4185 4862 6010 6255 Size: 481 1141 355 13 589 677 1148 245 Cuts at: 6255 1606 Size: 2120 Fragments arranged by size: 2120 1148 1141 677 589 481 355 245 13 NcoI C'CATG_G Cuts at: 513 1065 2004 3120 513 Size: 552 939 1116 4162 Fragments arranged by size: 4162 1116 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6769 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6769 NotI GC'GGCC_GC Cuts at: 2082 4216 2082 Size: 2134 4635 NspI r_CATG'y Cuts at: 1132 1229 3283 3415 4127 5672 1132 Size: 97 2054 132 712 1545 2229 Fragments arranged by size: 2229 2054 1545 712 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6769 PciI A'CATG_T Cuts at: 5668 5668 Size: 6769 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6769 PfoI T'CCnGG_A Cuts at: 3560 3560 Size: 6769 PmeI GTTT'AAAC Cuts at: 4073 4073 Size: 6769 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4838 4870 6351 6383 4838 Size: 32 1481 32 5224 Fragments arranged by size: 5224 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2317 2842 3568 2317 Size: 525 726 5518 Fragments arranged by size: 5518 726 525 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6769 PsiI TTA'TAA Cuts at: 3924 4339 3924 Size: 415 6354 PspOMI G'GGCC_C Cuts at: 2820 2895 2820 Size: 75 6694 PstI C_TGCA'G Cuts at: 830 4020 4121 830 Size: 3190 101 3478 Fragments arranged by size: 3478 3190 101 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6769 PvuII CAG'CTG Cuts at: 3583 3596 3583 Size: 13 6756 SacI G_AGCT'C Cuts at: 2071 2071 Size: 6769 SalI G'TCGA_C Cuts at: 1936 4111 1936 Size: 2175 4594 SanDI GG'GwC_CC Cuts at: 3568 3568 Size: 6769 SbfI CC_TGCA'GG Cuts at: 4121 4121 Size: 6769 ScaI AGT'ACT Cuts at: 5017 6729 5017 Size: 1712 5057 SfcI C'TryA_G Cuts at: 826 1045 2169 3738 4016 4117 5252 5933 6124 Size: 219 1124 1569 278 101 1135 681 191 Cuts at: 6124 826 Size: 1471 Fragments arranged by size: 1569 1471 1135 1124 681 278 219 191 101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6769 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6769 SmaI CCC'GGG Cuts at: 1872 4096 1872 Size: 2224 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 2870 3245 3284 4873 5774 Size: 197 926 864 63 375 39 1589 901 Cuts at: 5774 6036 820 Size: 262 1553 Fragments arranged by size: 1589 1553 926 901 864 375 262 197 63 39 SnaBI TAC'GTA Cuts at: 493 4560 493 Size: 4067 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6769 SphI G_CATG'C Cuts at: 1132 3283 4127 1132 Size: 2151 844 3774 Fragments arranged by size: 3774 2151 844 SspI AAT'ATT Cuts at: 5 52 4693 5 Size: 47 4641 2081 Fragments arranged by size: 4641 2081 47 StuI AGG'CCT Cuts at: 1517 3421 3716 1517 Size: 1904 295 4570 Fragments arranged by size: 4570 1904 295 StyI C'CwwG_G Cuts at: 513 1065 2004 3120 4208 513 Size: 552 939 1116 1088 3074 Fragments arranged by size: 3074 1116 1088 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2994 3037 3606 3722 4796 4813 4966 Size: 1255 1414 43 569 116 1074 17 153 Cuts at: 4966 5151 5596 325 Size: 185 445 1498 Fragments arranged by size: 1498 1414 1255 1074 569 445 185 153 116 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2878 5015 6727 Size: 274 80 33 51 1443 901 2137 1712 Cuts at: 6727 96 Size: 138 Fragments arranged by size: 2137 1712 1443 901 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2493 2525 3662 3694 6729 6761 2493 Size: 32 1137 32 3035 32 2501 Fragments arranged by size: 3035 2501 1137 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6769 XbaI T'CTAG_A Cuts at: 4105 4105 Size: 6769 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3730 3730 Size: 6769 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6769 XmaI C'CCGG_G Cuts at: 1870 4094 1870 Size: 2224 4545 XmnI GAAnn'nnTTC Cuts at: 3851 4898 3851 Size: 1047 5722 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4577 276 Size: 53 83 186 936 3043 2468 Fragments arranged by size: 3043 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI