(Linear) (Six-base) MAPSORT of: KIEE4191.seq Check: 2096 from: 1 to: 2064 KIEE4191.orf With 182 enzymes: SgfI * July 3, 2012 18:44 .. Acc65I G'GTAC_C Cuts at: 0 716 2064 Size: 716 1348 AflIII A'CryG_T Cuts at: 0 191 443 614 728 2064 Size: 191 252 171 114 1336 Fragments arranged by size: 1336 252 191 171 114 AleI CACnn'nnGTG Cuts at: 0 514 2064 Size: 514 1550 AlwNI CAG_nnn'CTG Cuts at: 0 1198 1363 2064 Size: 1198 165 701 Fragments arranged by size: 1198 701 165 ApaI G_GGCC'C Cuts at: 0 819 894 2064 Size: 819 75 1170 Fragments arranged by size: 1170 819 75 ApoI r'AATT_y Cuts at: 0 844 2064 Size: 844 1220 AvaI C'yCGr_G Cuts at: 0 649 2064 Size: 649 1415 BamHI G'GATC_C Cuts at: 0 1738 2064 Size: 1738 326 BanI G'GyrC_C Cuts at: 0 716 2064 Size: 716 1348 BanII G_rGCy'C Cuts at: 0 66 692 819 832 894 1555 1995 2064 Size: 66 626 127 13 62 661 440 69 Fragments arranged by size: 661 626 440 127 69 66 62 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 407 2064 Size: 407 1657 BbvCI CC'TCA_GC Cuts at: 0 83 316 1411 2045 2064 Size: 83 233 1095 634 19 Fragments arranged by size: 1095 634 233 83 19 BciVI GTATCCnnnnn_n' Cuts at: 0 743 2064 Size: 743 1321 BglI GCCn_nnn'nGGC Cuts at: 0 16 2064 Size: 16 2048 BlpI GC'TnA_GC Cuts at: 0 550 2064 Size: 550 1514 Bme1580I G_kGCm'C Cuts at: 0 819 894 2064 Size: 819 75 1170 Fragments arranged by size: 1170 819 75 BmgBI CAC'GTC Cuts at: 0 192 444 2064 Size: 192 252 1620 Fragments arranged by size: 1620 252 192 BmrI ACTGGGnnnn_n' Cuts at: 0 318 414 2064 Size: 318 96 1650 Fragments arranged by size: 1650 318 96 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 171 1583 1779 2064 Size: 171 1412 196 285 Fragments arranged by size: 1412 285 196 171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1513 1539 1900 2064 Size: 1513 26 361 164 Fragments arranged by size: 1513 361 164 26 Bpu10I CC'TnA_GC Cuts at: 0 65 83 316 595 1411 2045 2064 Size: 65 18 233 279 816 634 19 Fragments arranged by size: 816 634 279 233 65 19 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 787 850 1225 1300 2064 Size: 787 63 375 75 764 Fragments arranged by size: 787 764 375 75 63 BsaAI yAC'GTr Cuts at: 0 617 2064 Size: 617 1447 BsaHI Gr'CG_yC Cuts at: 0 1497 2064 Size: 1497 567 BsaWI w'CCGG_w Cuts at: 0 37 2064 Size: 37 2027 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1537 1567 2064 Size: 1537 30 497 Fragments arranged by size: 1537 497 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 100 103 106 667 1442 1644 2050 2064 Size: 100 3 3 561 775 202 406 14 Fragments arranged by size: 775 561 406 202 100 14 3 3 BseYI C'CCAG_C Cuts at: 0 818 1345 1587 2064 Size: 818 527 242 477 Fragments arranged by size: 818 527 477 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 925 2064 Size: 925 1139 BsiEI CG_ry'CG Cuts at: 0 80 1110 2064 Size: 80 1030 954 Fragments arranged by size: 1030 954 80 BsiHKAI G_wGCw'C Cuts at: 0 66 1886 2064 Size: 66 1820 178 Fragments arranged by size: 1820 178 66 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1504 2064 Size: 1504 560 Bsp1286I G_dGCh'C Cuts at: 0 66 692 819 832 894 1555 1886 1995 Size: 66 626 127 13 62 661 331 109 Cuts at: 1995 2064 Size: 69 Fragments arranged by size: 661 626 331 127 109 69 66 62 13 BspEI T'CCGG_A Cuts at: 0 37 2064 Size: 37 2027 BspHI T'CATG_A Cuts at: 0 1073 2064 Size: 1073 991 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 359 728 956 1190 2018 2064 Size: 359 369 228 234 828 46 Fragments arranged by size: 828 369 359 234 228 46 BsrBI CCG'CTC Cuts at: 0 155 759 2064 Size: 155 604 1305 Fragments arranged by size: 1305 604 155 BsrDI GCAATG_nn' Cuts at: 0 391 1911 2064 Size: 391 1520 153 Fragments arranged by size: 1520 391 153 BsrFI r'CCGG_y Cuts at: 0 269 2064 Size: 269 1795 BssSI C'ACGA_G Cuts at: 0 628 2064 Size: 628 1436 BstAPI GCAn_nnn'nTGC Cuts at: 0 948 2064 Size: 948 1116 BstXI CCAn_nnnn'nTGG Cuts at: 0 1353 1831 1878 2064 Size: 1353 478 47 186 Fragments arranged by size: 1353 478 186 47 BstYI r'GATC_y Cuts at: 0 168 668 1738 2064 Size: 168 500 1070 326 Fragments arranged by size: 1070 500 326 168 Bsu36I CC'TnA_GG Cuts at: 0 833 914 2064 Size: 833 81 1150 Fragments arranged by size: 1150 833 81 BtgI C'CryG_G Cuts at: 0 1115 2064 Size: 1115 949 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 614 2064 Size: 614 1450 BtsI GCAGTG_nn' Cuts at: 0 332 1645 2064 Size: 332 1313 419 Fragments arranged by size: 1313 419 332 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1110 1145 2064 Size: 1110 35 919 Fragments arranged by size: 1110 919 35 DrdI GACnn_nn'nnGTC Cuts at: 0 1570 2064 Size: 1570 494 EaeI y'GGCC_r Cuts at: 0 77 1085 1107 1118 1374 1463 1833 1868 Size: 77 1008 22 11 256 89 370 35 Cuts at: 1868 1928 2064 Size: 60 136 Fragments arranged by size: 1008 370 256 136 89 77 60 35 22 11 EagI C'GGCC_G Cuts at: 0 77 1107 2064 Size: 77 1030 957 Fragments arranged by size: 1030 957 77 EarI CTCTTCn'nnn_ Cuts at: 0 1213 2064 Size: 1213 851 EcoICRI GAG'CTC Cuts at: 0 64 2064 Size: 64 2000 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1513 1539 1900 2064 Size: 1513 26 361 164 Fragments arranged by size: 1513 361 164 26 EcoNI CCTnn'n_nnAGG Cuts at: 0 511 1186 1507 2064 Size: 511 675 321 557 Fragments arranged by size: 675 557 511 321 EcoO109I rG'GnC_Cy Cuts at: 0 17 312 837 910 1480 1563 2064 Size: 17 295 525 73 570 83 501 Fragments arranged by size: 570 525 501 295 83 73 17 EcoRI G'AATT_C Cuts at: 0 844 2064 Size: 844 1220 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 258 2064 Size: 258 1806 FspI TGC'GCA Cuts at: 0 880 1903 2064 Size: 880 1023 161 Fragments arranged by size: 1023 880 161 HaeII r_GCGC'y Cuts at: 0 46 940 1199 2064 Size: 46 894 259 865 Fragments arranged by size: 894 865 259 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 448 480 997 1029 1663 1695 2064 Size: 448 32 517 32 634 32 369 Fragments arranged by size: 634 517 448 369 32 32 32 HincII GTy'rAC Cuts at: 0 1455 2064 Size: 1455 609 KpnI G_GTAC'C Cuts at: 0 720 2064 Size: 720 1344 MfeI C'AATT_G Cuts at: 0 355 2064 Size: 355 1709 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 464 564 1317 2064 Size: 464 100 753 747 Fragments arranged by size: 753 747 464 100 MscI TGG'CCA Cuts at: 0 1120 1376 1465 1870 1930 2064 Size: 1120 256 89 405 60 134 Fragments arranged by size: 1120 405 256 134 89 60 MslI CAynn'nnrTG Cuts at: 0 514 680 2064 Size: 514 166 1384 Fragments arranged by size: 1384 514 166 MspA1I CmG'CkG Cuts at: 0 82 1223 1578 1591 2064 Size: 82 1141 355 13 473 Fragments arranged by size: 1141 473 355 82 13 NcoI C'CATG_G Cuts at: 0 1115 2064 Size: 1115 949 NotI GC'GGCC_GC Cuts at: 0 77 2064 Size: 77 1987 NspI r_CATG'y Cuts at: 0 1278 1410 2064 Size: 1278 132 654 Fragments arranged by size: 1278 654 132 PfoI T'CCnGG_A Cuts at: 0 1555 2064 Size: 1555 509 PpuMI rG'GwC_Cy Cuts at: 0 312 837 1563 2064 Size: 312 525 726 501 Fragments arranged by size: 726 525 501 312 PsiI TTA'TAA Cuts at: 0 1919 2064 Size: 1919 145 PspOMI G'GGCC_C Cuts at: 0 815 890 2064 Size: 815 75 1174 Fragments arranged by size: 1174 815 75 PstI C_TGCA'G Cuts at: 0 2015 2064 Size: 2015 49 PvuII CAG'CTG Cuts at: 0 1578 1591 2064 Size: 1578 13 473 Fragments arranged by size: 1578 473 13 SacI G_AGCT'C Cuts at: 0 66 2064 Size: 66 1998 SanDI GG'GwC_CC Cuts at: 0 1563 2064 Size: 1563 501 SfcI C'TryA_G Cuts at: 0 164 1733 2011 2064 Size: 164 1569 278 53 Fragments arranged by size: 1569 278 164 53 SmlI C'TyrA_G Cuts at: 0 802 865 1240 1279 2064 Size: 802 63 375 39 785 Fragments arranged by size: 802 785 375 63 39 SphI G_CATG'C Cuts at: 0 1278 2064 Size: 1278 786 StuI AGG'CCT Cuts at: 0 1416 1711 2064 Size: 1416 295 353 Fragments arranged by size: 1416 353 295 StyI C'CwwG_G Cuts at: 0 1115 2064 Size: 1115 949 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 989 1032 1601 1717 2064 Size: 989 43 569 116 347 Fragments arranged by size: 989 569 347 116 43 TatI w'GTAC_w Cuts at: 0 873 2064 Size: 873 1191 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 488 520 1657 1689 2064 Size: 488 32 1137 32 375 Fragments arranged by size: 1137 488 375 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1725 2064 Size: 1725 339 XmnI GAAnn'nnTTC Cuts at: 0 1846 2064 Size: 1846 218 Enzymes that do cut: Acc65I AflIII AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I NcoI NotI NspI PfoI PpuMI PsiI PspOMI PstI PvuII SacI SanDI SfcI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI BglII BmtI BsaI BsaBI BsiWI BsmI BsrGI BssHII BstBI BstEII BstZ17I ClaI DraI DraIII EciI EcoRV FseI FspAI HindIII HpaI KasI MluI NaeI NarI NdeI NgoMIV NheI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmaI ZraI