################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## Acc65I AcsI AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbvCI BclI BfaI BlnI BsiEI BsiWI BspDI BspHI BspMI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI KpnI MaeI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RcaI RsrII SacI SalI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 1288 GTCTAC GT^[AC][GT]AC AclI 2766 AACGTT AA^CGTT AflII 297 CTTAAG C^TTAAG ApaI 2567 GGGCCC GGGCC^C BamHI 2759 GGATCC G^GATCC BbeI 2182 GGCGCC GGCGC^C BbrPI 673 CACGTG CAC^GTG BciVI 587 GGATAC GTATCC(6/5) BfrI 297 CTTAAG C^TTAAG BglII 1116 AGATCT A^GATCT BsaAI 673 CACGTG [CT]AC^GT[GA] BsaBI 588 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 2199 CGTCTC CGTCTC(1/5) BsmI 453 GCATTC GAATGC(1/-1) Bsp1407I 677 TGTACA T^GTACA BsrDI 1359 CATTGC GCAATG(2/0) BsrGI 677 TGTACA T^GTACA DraIII 2189 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1407 GACTGCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HaeII 2182 GGCGCC [GA]GCGC^[CT] HincII 541 GTTAAC GT[CT]^[GA]AC HindII 541 GTTAAC GT[CT]^[GA]AC HindIII 1888 AAGCTT A^AGCTT HpaI 541 GTTAAC GTT^AAC KasI 2182 GGCGCC G^GCGCC KspI 1866 CCGCGG CCGC^GG MamI 588 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 2182 GGCGCC GG^CGCC NcoI 2699 CCATGG C^CATGG PflMI 659 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 673 CACGTG CAC^GTG PmlI 673 CACGTG CAC^GTG Psp1406I 2766 AACGTT AA^CGTT SacII 1866 CCGCGG CCGC^GG SbfI 2455 CCTGCAGG CCTGCA^GG SfoI 2182 GGCGCC GGC^GCC SmaI 2314 CCCGGG CCC^GGG Sse8387I 2455 CCTGCAGG CCTGCA^GG SspBI 677 TGTACA T^GTACA SspI 521 AATATT AAT^ATT Van91I 659 CCATGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2314 CCCGGG C^CCGGG XmaI 2314 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AccII 1614 CGCG CG^CG 1867 CGCG AccIII 1042 TCCGGA T^CCGGA 1223 TCCGGA AspHI 220 GTGCTC G[AT]GC[AT]^C 719 GTGCTC BcgI 1581 GCAGCCGTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2214 GCAGACGGATCG BlpI 2301 GCTCAGC GC^T[ACGT]AGC 2364 GCTCAGC Bpu1102I 2301 GCTCAGC GC^T[ACGT]AGC 2364 GCTCAGC BsaWI 1042 TCCGGA [AT]^CCGG[AT] 1223 TCCGGA BseAI 1042 TCCGGA T^CCGGA 1223 TCCGGA BsiHKAI 220 GTGCTC G[AT]GC[AT]^C 719 GTGCTC BspEI 1042 TCCGGA T^CCGGA 1223 TCCGGA BspLU11I 548 ACATGT A^CATGT 1506 ACATGT BssSI 2328 CACGAG CACGAG(-5/-1) 2712 CACGAG BstAPI 1056 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1644 GCAGCAAGTGC BstUI 1614 CGCG CG^CG 1867 CGCG CelII 2301 GCTCAGC GC^T[ACGT]AGC 2364 GCTCAGC HgaI 2338 GACGC GACGC(5/10) 2599 GCGTC MroI 1042 TCCGGA T^CCGGA 1223 TCCGGA MvnI 1614 CGCG CG^CG 1867 CGCG NaeI 2179 GCCGGC GCC^GGC 2589 GCCGGC NgoMIV 2179 GCCGGC G^CCGGC 2589 GCCGGC NspI 548 ACATGT [GA]CATG^[CT] 1506 ACATGT StuI 2827 AGGCCT AGG^CCT 2868 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AatII AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII