Restriction map for
pFN21AE4254
(Circular) (Six-base) MAPSORT of: pFN21AE4254.seq Check: 947 from: 1 to: 7732
pFN21AE4254 7732 bp
With 182 enzymes: SgfI *
March 28, 2012 13:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3563 3726 4206 5542
Size: 53 83 186 936 2027 163 480 1336
Cuts at: 5542 278
Size: 2468
Fragments arranged by size:
2468 2027 1336 936 480 186 163 83
53
AccI GT'mk_AC
Cuts at: 1937 3294 5075 1937
Size: 1357 1781 4594
Fragments arranged by size:
4594 1781 1357
Acc65I G'GTAC_C
Cuts at: 5053 5053
Size: 7732
AclI AA'CG_TT
Cuts at: 1563 4772 5859 6232 1563
Size: 3209 1087 373 3063
Fragments arranged by size:
3209 3063 1087 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 2903 3712 5795 7179 7447 1632
Size: 627 644 809 2083 1384 268 1917
Fragments arranged by size:
2083 1917 1384 809 644 627 268
AflII C'TTAA_G
Cuts at: 820 1017 2302 820
Size: 197 1285 6250
Fragments arranged by size:
6250 1285 197
AflIII A'CryG_T
Cuts at: 1203 2553 2679 3511 6631 7608 1203
Size: 1350 126 832 3120 977 1327
Fragments arranged by size:
3120 1350 1327 977 832 126
AgeI A'CCGG_T
Cuts at: 6563 6563
Size: 7732
AhdI GACnn_n'nnGTC
Cuts at: 6461 6461
Size: 7732
AleI CACnn'nnGTG
Cuts at: 1166 2222 1166
Size: 1056 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4914 1184
Size: 3730 4002
AlwNI CAG_nnn'CTG
Cuts at: 2096 3067 3674 3763 3844 4735 4822 5475 7047
Size: 971 607 89 81 891 87 653 1572
Cuts at: 7047 2096
Size: 2781
Fragments arranged by size:
2781 1572 971 891 653 607 89 87
81
ApaI G_GGCC'C
Cuts at: 4576 4576
Size: 7732
ApaLI G'TGCA_C
Cuts at: 5789 6945 5789
Size: 1156 6576
ApoI r'AATT_y
Cuts at: 1493 5041 5267 5419 7528 1493
Size: 3548 226 152 2109 1697
Fragments arranged by size:
3548 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6286 160
Size: 872 5254 1606
Fragments arranged by size:
5254 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7732
AvaI C'yCGr_G
Cuts at: 1870 1943 3995 4319 4963 5057 1870
Size: 73 2052 324 644 94 4545
Fragments arranged by size:
4545 2052 644 324 94 73
BamHI G'GATC_C
Cuts at: 4764 5062 5429 4764
Size: 298 367 7067
Fragments arranged by size:
7067 367 298
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4069 4187 4709 5053 6508
Size: 325 217 627 2282 118 522 344 1455
Cuts at: 6508 618
Size: 1842
Fragments arranged by size:
2282 1842 1455 627 522 344 325 217
118
BanII G_rGCy'C
Cuts at: 1391 2012 3575 4260 4576 4724 4964 5051 1391
Size: 621 1563 685 316 148 240 87 4072
Fragments arranged by size:
4072 1563 685 621 316 240 148 87
BbeI G_GCGC'C
Cuts at: 4191 4191
Size: 7732
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3293 3465 3718 3726 4127 928
Size: 971 1394 172 253 8 401 4533
Fragments arranged by size:
4533 1394 971 401 253 172 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3575 3609 4208 4242 5923 5957 1903
Size: 34 1638 34 599 34 1681 34 3678
Fragments arranged by size:
3678 1681 1638 599 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2586 5624 6834 2586
Size: 3038 1210 3484
Fragments arranged by size:
3484 3038 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7732
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2205 4021 4861
Size: 107 122 71 631 605 533 1816 840
Cuts at: 4861 6343 136
Size: 1482 1525
Fragments arranged by size:
1816 1525 1482 840 631 605 533 122
107 71
BglII A'GATC_T
Cuts at: 3121 7727 3121
Size: 4606 3126
BlpI GC'TnA_GC
Cuts at: 4307 4370 5149 4307
Size: 63 779 6890
Fragments arranged by size:
6890 779 63
Bme1580I G_kGCm'C
Cuts at: 1790 3799 4074 4576 4642 4712 5793 6949 1790
Size: 2009 275 502 66 70 1081 1156 2573
Fragments arranged by size:
2573 2009 1156 1081 502 275 70 66
BmgBI CAC'GTC
Cuts at: 1318 2869 4957 1318
Size: 1551 2088 4093
Fragments arranged by size:
4093 2088 1551
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2925 3773 4047
Size: 416 53 83 399 24 1508 848 274
Cuts at: 4047 5463 6421 442
Size: 1416 958 1753
Fragments arranged by size:
1753 1508 1416 958 848 416 399 274
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7732
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 4000 2957
Size: 1043 6689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1996 2834 3940 4160 4283 4563 4857 4898
Size: 757 838 1106 220 123 280 294 41
Cuts at: 4898 6392 1239
Size: 1494 2579
Fragments arranged by size:
2579 1494 1106 838 757 294 280 220
123 41
Bpu10I CC'TnA_GC
Cuts at: 3576 5006 3576
Size: 1430 6302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2927 4774 4854 5857 6722 7020 2927
Size: 1847 80 1003 865 298 3639
Fragments arranged by size:
3639 1847 1003 865 298 80
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2027 2908 3517 4552 4656 4802 4833
Size: 617 528 881 609 1035 104 146 31
Cuts at: 4833 4911 5015 6395 882
Size: 78 104 1380 2219
Fragments arranged by size:
2219 1380 1035 881 617 609 528 146
104 104 78 31
BsaAI yAC'GTr
Cuts at: 493 1204 2680 5523 493
Size: 711 1476 2843 2702
Fragments arranged by size:
2843 2702 1476 711
BsaBI GATnn'nnATC
Cuts at: 2597 7726 2597
Size: 5129 2603
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3560 3723 4188 4203
Size: 53 83 186 936 2027 163 465 15
Cuts at: 4203 4344 5539 5921 6556 275
Size: 141 1195 382 635 1451
Fragments arranged by size:
2027 1451 1195 936 635 465 382 186
163 141 83 53 15
BsaWI w'CCGG_w
Cuts at: 3047 3228 6165 6563 6837 6984 7395 3047
Size: 181 2937 398 274 147 411 3384
Fragments arranged by size:
3384 2937 411 398 274 181 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2427 2457 3029 3059 1178
Size: 30 165 30 1024 30 572 30 5851
Fragments arranged by size:
5851 1024 572 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3014 3880 4079 4893 1186
Size: 1828 866 199 814 4025
Fragments arranged by size:
4025 1828 866 814 199
BseYI C'CCAG_C
Cuts at: 2495 3679 3950 3961 4132 4735 4744 6935 7431
Size: 1184 271 11 171 603 9 2191 496
Cuts at: 7431 2495
Size: 2796
Fragments arranged by size:
2796 2191 1184 603 496 271 171 11
9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2123 2393 3399 1163
Size: 573 387 270 1006 5496
Fragments arranged by size:
5496 1006 573 387 270
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5182 5943 6971 1540
Size: 275 186 3181 761 1028 2301
Fragments arranged by size:
3181 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2229 2728 5793 5878 6949 2229
Size: 499 3065 85 1071 3012
Fragments arranged by size:
3065 3012 1071 499 85
BsmI GAATG_Cn'
Cuts at: 2458 5243 5336 2458
Size: 2785 93 4854
Fragments arranged by size:
4854 2785 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4210 4210
Size: 7732
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2229 2728 3575 3799 4074 4260
Size: 399 222 217 499 847 224 275 186
Cuts at: 4260 4576 4642 4712 4724 4964 5051 5793 5878
Size: 316 66 70 12 240 87 742 85
Cuts at: 5878 6949 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 847 742 499 399 316 275
240 224 222 217 186 87 85 70
66 12
BspEI T'CCGG_A
Cuts at: 3047 3228 7395 3047
Size: 181 4167 3384
Fragments arranged by size:
4167 3384 181
BspHI T'CATG_A
Cuts at: 5621 7351 5621
Size: 1730 6002
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5087 844
Size: 4243 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3045 4213 4306 4369 4788 5619 1394
Size: 429 1222 1168 93 63 419 831 3507
Fragments arranged by size:
3507 1222 1168 831 429 419 93 63
BsrDI GCAATG_nn'
Cuts at: 65 1248 3363 6227 6401 65
Size: 1183 2115 2864 174 1396
Fragments arranged by size:
2864 2115 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3462 4184 4594 6376 6563 1700
Size: 110 1652 722 410 1782 187 2869
Fragments arranged by size:
2869 1782 1652 722 410 187 110
BsrGI T'GTAC_A
Cuts at: 96 2682 96
Size: 2586 5146
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7732
BssSI C'ACGA_G
Cuts at: 4333 4717 5792 6804 4333
Size: 384 1075 1012 5261
Fragments arranged by size:
5261 1075 1012 384
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3067 3655 794
Size: 2273 588 4871
Fragments arranged by size:
4871 2273 588
BstBI TT'CG_AA
Cuts at: 6536 6536
Size: 7732
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7732
BstYI r'GATC_y
Cuts at: 1972 2092 3121 3847 4764 5062 5429 5814 5831
Size: 120 1029 726 917 298 367 385 17
Cuts at: 5831 7283 7369 7381 7727 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1029 917 726 385 367 346
298 120 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2097 3809 4829 2097
Size: 1712 1020 5000
Fragments arranged by size:
5000 1712 1020
BtgI C'CryG_G
Cuts at: 513 1065 2004 2638 3871 4704 513
Size: 552 939 634 1233 833 3541
Fragments arranged by size:
3541 1233 939 833 634 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2666 3523 487
Size: 683 56 1440 857 4696
Fragments arranged by size:
4696 1440 857 683 56
BtsI GCAGTG_nn'
Cuts at: 3199 3549 3752 4345 4886 5021 5248 6042 6062
Size: 350 203 593 541 135 227 794 20
Cuts at: 6062 3199
Size: 4869
Fragments arranged by size:
4869 794 593 541 350 227 203 135
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7732
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7697
DraI TTT'AAA
Cuts at: 5036 5036
Size: 7732
DraIII CAC_nnn'GTG
Cuts at: 4199 4199
Size: 7732
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3418 6739 809
Size: 507 2102 3321 1802
Fragments arranged by size:
3321 2102 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2206 2928 3279
Size: 54 1425 50 275 18 376 722 351
Cuts at: 3279 3467 3942 4167 4693 5179 6068 7697 8
Size: 188 475 225 526 486 889 1629 43
Fragments arranged by size:
1629 1425 889 722 526 486 475 376
351 275 225 188 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5179 1537
Size: 275 3367 4090
Fragments arranged by size:
4090 3367 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2279 2881 3098 3156 3390 5662 1353
Size: 926 602 217 58 234 2272 3423
Fragments arranged by size:
3423 2272 926 602 234 217 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4657 6319 6693 6839 387
Size: 1064 138 3068 1662 374 146 1280
Fragments arranged by size:
3068 1662 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1996 2259 2834 2903 3712 3940 4160
Size: 393 364 263 575 69 809 228 220
Cuts at: 4160 4283 4563 4857 4898 5795 6392 7179 7447
Size: 123 280 294 41 897 597 787 268
Cuts at: 7447 1239
Size: 1524
Fragments arranged by size:
1524 897 809 787 597 575 393 364
294 280 268 263 228 220 123 69
41
EcoNI CCTnn'n_nnAGG
Cuts at: 2068 2430 3929 2068
Size: 362 1499 5871
Fragments arranged by size:
5871 1499 362
EcoO109I rG'GnC_Cy
Cuts at: 3442 3671 3787 3825 4023 4283 4572 4573 4739
Size: 229 116 38 198 260 289 1 166
Cuts at: 4739 4863 3442
Size: 124 6311
Fragments arranged by size:
6311 289 260 229 198 166 124 116
38 1
EcoRI G'AATT_C
Cuts at: 5041 5419 5041
Size: 378 7354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 7732
FspI TGC'GCA
Cuts at: 6238 6238
Size: 7732
HaeII r_GCGC'y
Cuts at: 1678 4191 6879 1678
Size: 2513 2688 2531
Fragments arranged by size:
2688 2531 2513
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2427 2459 2858 2890 3832
Size: 32 135 32 855 32 399 32 942
Cuts at: 3832 3864 4951 4983 6373 6405 6447 6479 1373
Size: 32 1087 32 1390 32 42 32 2626
Fragments arranged by size:
2626 1390 1087 942 855 399 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2548 5076 5322 669
Size: 988 281 610 2528 246 3079
Fragments arranged by size:
3079 2528 988 610 281 246
HindIII A'AGCT_T
Cuts at: 748 3893 748
Size: 3145 4587
HpaI GTT'AAC
Cuts at: 2548 5322 2548
Size: 2774 4958
KasI G'GCGC_C
Cuts at: 4187 4187
Size: 7732
KpnI G_GTAC'C
Cuts at: 5057 5057
Size: 7732
MfeI C'AATT_G
Cuts at: 5331 5331
Size: 7732
MluI A'CGCG_T
Cuts at: 7608 7608
Size: 7732
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6846 7030 1305
Size: 5541 184 2007
Fragments arranged by size:
5541 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2208 2930 3944 4695 10
Size: 54 1425 343 376 722 1014 751 3047
Fragments arranged by size:
3047 1425 1014 751 722 376 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 2690 3542 5690 6049 6208 518
Size: 648 1056 468 852 2148 359 159 2042
Fragments arranged by size:
2148 2042 1056 852 648 468 359 159
MspA1I CmG'CkG
Cuts at: 1606 3085 3403 3873 3965 4735 5148 5825 6973
Size: 1479 318 470 92 770 413 677 1148
Cuts at: 6973 7218 1606
Size: 245 2120
Fragments arranged by size:
2120 1479 1148 770 677 470 413 318
245 92
NaeI GCC'GGC
Cuts at: 4186 4596 4186
Size: 410 7322
NarI GG'CG_CC
Cuts at: 4188 4188
Size: 7732
NcoI C'CATG_G
Cuts at: 513 1065 2004 4704 513
Size: 552 939 2700 3541
Fragments arranged by size:
3541 2700 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7732
NgoMIV G'CCGG_C
Cuts at: 4184 4594 4184
Size: 410 7322
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7732
NotI GC'GGCC_GC
Cuts at: 5179 5179
Size: 7732
NspI r_CATG'y
Cuts at: 1132 1229 2557 3515 5090 6635 1132
Size: 97 1328 958 1575 1545 2229
Fragments arranged by size:
2229 1575 1545 1328 958 97
PasI CC'CwG_GG
Cuts at: 1348 4588 1348
Size: 3240 4492
PciI A'CATG_T
Cuts at: 2553 3511 6631 2553
Size: 958 3120 3654
Fragments arranged by size:
3654 3120 958
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2670 1108
Size: 1562 6170
PfoI T'CCnGG_A
Cuts at: 2012 2115 4359 2012
Size: 103 2244 5385
Fragments arranged by size:
5385 2244 103
PmeI GTTT'AAAC
Cuts at: 5036 5036
Size: 7732
PmlI CAC'GTG
Cuts at: 2680 2680
Size: 7732
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5801 5833 7314 7346 5801
Size: 32 1481 32 6187
Fragments arranged by size:
6187 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3442 3787 3825 4739 3442
Size: 345 38 914 6435
Fragments arranged by size:
6435 914 345 38
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7732
PsiI TTA'TAA
Cuts at: 2334 5302 2334
Size: 2968 4764
PspOMI G'GGCC_C
Cuts at: 4572 4572
Size: 7732
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2577 2609 2577
Size: 32 7700
PstI C_TGCA'G
Cuts at: 830 3072 3090 3759 4221 4330 4465 4893 5084
Size: 2242 18 669 462 109 135 428 191
Cuts at: 5084 830
Size: 3478
Fragments arranged by size:
3478 2242 669 462 428 191 135 109
18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7732
PvuII CAG'CTG
Cuts at: 3085 3403 3965 3085
Size: 318 562 6852
Fragments arranged by size:
6852 562 318
SacII CC_GC'GG
Cuts at: 3874 3874
Size: 7732
SalI G'TCGA_C
Cuts at: 1936 5074 1936
Size: 3138 4594
SanDI GG'GwC_CC
Cuts at: 4739 4739
Size: 7732
SapI GCTCTTCn'nnn_
Cuts at: 2279 3098 3390 2279
Size: 819 292 6621
Fragments arranged by size:
6621 819 292
SbfI CC_TGCA'GG
Cuts at: 4465 5084 4465
Size: 619 7113
ScaI AGT'ACT
Cuts at: 5980 7692 5980
Size: 1712 6020
SfcI C'TryA_G
Cuts at: 826 1045 2125 2144 3068 3086 3755 4217 4326
Size: 219 1080 19 924 18 669 462 109
Cuts at: 4326 4461 4727 4889 5080 6215 6896 7087 826
Size: 135 266 162 191 1135 681 191 1471
Fragments arranged by size:
1471 1135 1080 924 681 669 462 266
219 191 191 162 135 109 19 18
SfoI GGC'GCC
Cuts at: 4189 4189
Size: 7732
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7732
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7732
SmaI CCC'GGG
Cuts at: 1872 4321 5059 1872
Size: 2449 738 4545
Fragments arranged by size:
4545 2449 738
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2302 2942 4789 4869 5836 6737
Size: 197 926 359 640 1847 80 967 901
Cuts at: 6737 6999 820
Size: 262 1553
Fragments arranged by size:
1847 1553 967 926 901 640 359 262
197 80
SnaBI TAC'GTA
Cuts at: 493 5523 493
Size: 5030 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7732
SphI G_CATG'C
Cuts at: 1132 5090 1132
Size: 3958 3774
SspI AAT'ATT
Cuts at: 5 52 2528 5656 5
Size: 47 2476 3128 2081
Fragments arranged by size:
3128 2476 2081 47
StuI AGG'CCT
Cuts at: 1517 4834 4875 1517
Size: 3317 41 4374
Fragments arranged by size:
4374 3317 41
StyI C'CwwG_G
Cuts at: 513 1065 2004 3438 4111 4279 4704 5171 513
Size: 552 939 1434 673 168 425 467 3074
Fragments arranged by size:
3074 1434 939 673 552 467 425 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4486 4546 4727 5759 5776 5929 6114
Size: 1255 2906 60 181 1032 17 153 185
Cuts at: 6114 6559 325
Size: 445 1498
Fragments arranged by size:
2906 1498 1255 1032 445 185 181 153
60 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2667 2682 5978
Size: 274 80 33 51 1443 690 15 3296
Cuts at: 5978 7690 96
Size: 1712 138
Fragments arranged by size:
3296 1712 1443 690 274 138 80 51
33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 3029 3061 3223 3255 7692 7724 2369
Size: 32 628 32 162 32 4437 32 2377
Fragments arranged by size:
4437 2377 628 162 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7732
XbaI T'CTAG_A
Cuts at: 5068 5068
Size: 7732
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4081 4540 4819 4081
Size: 459 279 6994
Fragments arranged by size:
6994 459 279
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7732
XmaI C'CCGG_G
Cuts at: 1870 4319 5057 1870
Size: 2449 738 4545
Fragments arranged by size:
4545 2449 738
XmnI GAAnn'nnTTC
Cuts at: 5861 5861
Size: 7732
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3561 3724 4204 5540
Size: 53 83 186 936 2027 163 480 1336
Cuts at: 5540 276
Size: 2468
Fragments arranged by size:
2468 2027 1336 936 480 186 163 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BsiWI BstXI BstZ17I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PspXI RsrII SacI SexAI SfiI SrfI
SwaI