################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccI AccIII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp718I AspI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BseRI BsiEI BsiWI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI DsaI EagI EclXI Eco47III Eco52I Eco81I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NciI NcoI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp45I Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 1058 CGCG CG^CG AclI 1462 AACGTT AA^CGTT AflII 49 CTTAAG C^TTAAG AflIII 1193 ACATGT A^C[GA][CT]GT AhdI 1150 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 793 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 272 GGGCCC GGGCC^C AspEI 1150 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 584 GTGCTC G[AT]GC[AT]^C AvaI 893 CTCGAG C^[CT]CG[GA]G BalI 1200 TGGCCA TGG^CCA BfaI 1147 CTAG C^TAG BfrI 49 CTTAAG C^TTAAG BglII 744 AGATCT A^GATCT BsaBI 1180 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1238 GGTCTC GGTCTC(1/5) BsgI 921 GTGCAG GTGCAG(16/14) BsiHKAI 584 GTGCTC G[AT]GC[AT]^C BsmBI 694 CGTCTC CGTCTC(1/5) BsmFI 805 GTCCC GGGAC(10/14) BsmI 539 GAATGC GAATGC(1/-1) BsoBI 893 CTCGAG C^[CT]CG[GA]G BspLU11I 1193 ACATGT A^CATGT BssSI 626 CTCGTG CACGAG(-5/-1) BstEII 333 GGTTACC G^GT[ACGT]ACC BstPI 333 GGTTACC G^GT[ACGT]ACC BstUI 1058 CGCG CG^CG BstXI 1334 CCAAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 271 GGGGCCC [GA]G^G[ACGT]CC[CT] Eam1105I 1150 GACGCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 1208 CCTCTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 271 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoO65I 333 GGTTACC G^GT[ACGT]ACC EcoRI 68 GAATTC G^AATTC EcoRV 862 GATATC GAT^ATC EcoT14I 267 CCAAGG C^C[AT][AT]GG EcoT22I 541 ATGCAT ATGCA^T MaeI 1147 CTAG C^TAG MaeII 1463 ACGT A^CGT MamI 1180 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1200 TGGCCA TGG^CCA MscI 1200 TGGCCA TGG^CCA MslI 867 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 1058 CGCG CG^CG NaeI 966 GCCGGC GCC^GGC NgoMIV 966 GCCGGC G^CCGGC NsiI 541 ATGCAT ATGCA^T NspI 1193 ACATGT [GA]CATG^[CT] PaeR7I 893 CTCGAG C^TCGAG Psp1406I 1462 AACGTT AA^CGTT StuI 242 AGGCCT AGG^CCT StyI 267 CCAAGG C^C[AT][AT]GG XcmI 521 CCAAGCTGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 893 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 168 GTAC GT^AC 681 GTAC Asp700I 345 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 348 GAAATTCTTC BanII 272 GGGCCC G[GA]GC[CT]^C 770 GAGCCC BspHI 686 TCATGA T^CATGA 1322 TCATGA BsrFI 425 ACCGGC [GA]^CCGG[CT] 966 GCCGGC CfoI 777 GCGC GCG^C 1059 GCGC Cfr10I 425 ACCGGC [GA]^CCGG[CT] 966 GCCGGC Cfr13I 272 GGGCC G^G[ACGT]CC 273 GGCCC DraIII 601 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG 915 CACCAAGTG EaeI 608 TGGCCG [CT]^GGCC[GA] 1200 TGGCCA EarI 163 GAAGAG CTCTTC(1/4) 238 GAAGAG FauI 326 CCCGC CCCGC(4/6) 228 GCGGG HapII 426 CCGG C^CGG 967 CCGG HhaI 777 GCGC GCG^C 1059 GCGC HinP1I 777 GCGC G^CGC 1059 GCGC HpaII 426 CCGG C^CGG 967 CCGG Ksp632I 163 GAAGAG CTCTTC(1/4) 238 GAAGAG MspA1I 226 CAGCGG C[AC]G^C[GT]G 974 CAGCGG MspI 426 CCGG C^CGG 967 CCGG NlaIV 271 GGGGCC GG[ACGT]^[ACGT]CC 272 GGGCCC RcaI 686 TCATGA T^CATGA 1322 TCATGA RsaI 168 GTAC GT^AC 681 GTAC Sau96I 272 GGGCC G^G[ACGT]CC 273 GGCCC TaqI 653 TCGA T^CGA 894 TCGA TthHB8I 653 TCGA T^CGA 894 TCGA XmnI 345 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 348 GAAATTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 57 enzymes) # ######################################### AciI AcsI AcyI AluI AlwI ApoI BbsI BbvI BmyI BpmI BpuAI BsaHI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HgaI Hin1I HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII PflMI PleI Sau3AI ScrFI SfaNI SmlI TfiI Tru9I TseI Tsp509I TspRI Van91I XhoII